Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

HAYATI Journal of Biosciences

hayati
Website

Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

Arjuna Subject : -

Articles 8 Documents

Search results for , issue "Vol. 12 No. 3 (2005): September 2005" : 8 Documents clear

Solubilization, Activation and Partial Purification of a Sialidase from Horse Liver KRISHNA PURNAWAN CANDRA; PETER ROGGENTIN; ROLAND SCHAUER
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

Using sialyl-methylumbelliferyl -glycoside as substrate, sialidase in horse liver was detected as a membrane-bound enzyme. A yield of about 50% of sialidase activity was found in supernatant when solubilized in 0.1 M sodium-phosphate buffer pH 5.5, containing 0.15 M NaCl, 0.25 M sucrose, and 0.5% Triton X-100. Sialidase in the solubilisate could be activated by incubating in acidic pH at 37 oC. Incubation of this solubilized enzyme at 37 oC for 1.5 h at pH 5.0 led to 10% increase of activity and to the precipitation of about 50% of contaminating protein. Using cation-exchange chromatography on S-Sepharose FF and affinity chromatography on p-aminophenyl oxamic acid-agarose following solubilization and activation, about 6% of total sialidase activity was recovered with the purification factor of about 500. The pH and temperature optimum were measured at pH 4.3 and between 37-45 oC, respectively. Neu5Ac2en was a strong inhibitor, while p-aminophenyl oxamic acid had only a weak inhibitory effect.

Horizontal Gene Transfer and Population of Phyllosphere Bacteria on Transgenic and Nontransgenic Cotton ROHANI CINTA BADIA GINTING; ANTONIUS SUWANTO; ARIS TJAHJOLEKSONO
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

The possibility of horizontal gene transfer of plant genomic DNA and bacteria in the soil, particularly as this relates to the possible transfer of genes encoding antibiotic resistance, has been seen as hazard associated with genetically engineered plants. It is hypothesized that introduction of bacterial genes into the plant genome leads to a higher probability of gene transfer from plants to bacteria due to the presence of homologous sequences. Bollgard (BG) cotton was constructed through the introduction of cry1A(c) gene, encodes for insecticidal activity againts Lepidopteran pests, together with genes for spectinomycin/streptomycin resistant (aad) and kanamycin resistant (nptII), into the genome of a conventional cotton variety, Delta Pine (DP). The aim of this study were to evaluate the ability of naturally competent Acinetobacter calcoaceticus strain ADP1 to take up and integrate transgenic plant DNA based on homologous recombination under optimized laboratory condition, and to compare phyllosphere microbial population resistant to antibiotic on leaves of transgenic and nontransgenic plant. The results showed that transformation of ADP1 cells with Bollgard DNA was not detected on nitrocellulose membrane nor in sterile soil. Total phyllosphere bacterial population on leaves collected from one month after planting were 1.3 x 108 and 1.6 x 108 cfu/g leave fresh weight for BG and DP, respectively. Samples collected after three month contained 5.9 x 107 and 7.1 x 107 cfu/g leave fresh weight for BG and DP, respectively. This study also showed that there was no significant difference of phyllosphere bacterial population resistant to streptomycin and kanamycin on leaves of BG or DP samples collected from one or three month after planting.

Genetic Parameter Estimation on Upland Rice Agronomic Characters in Ultisols through Diallel Analysis MARIA AGUSTINA; SURJONO HADI SUTJAHJO; TRI KOESOEMANINGTYAS; YUSURUM JAGAU
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

An estimate of genetic parameters was made to study the types of gene action in rice. A half diallel analysis was performed among seven rice genotypes with different genetical backgrounds, i.e. Situgintung, Jatiluhur, Gajah Mungkur, B8503E-TB-9-0-3, Krowal, CT6510-24-1-3, IR 64, and its 21 progenies. This experimental material was arranged in a complete block design with four replications. All observed characters had highly significant genotypic differences. The genetic parameters were estimated following Hayman method. The simple model of additive-dominance was sufficiently detected through the absence of nonalellic interaction. Productive tillers exhibited partial dominance. Heritability in a narrow sense was high. There was a positive correlation between grain yield and the number of productive tillers.

Taurocholate Deconjugation and Cholesterol Binding by Indigenous Dadih Lactic Acid Bacteria USMAN PATO; MUHAMMAD ALI; ABDUL K. PARLINDUNGAN
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

High serum cholesterol levels have been associated with an increased risk for human coronary heart disease. Lowering of serum cholesterol has been suggested to prevent the heart disease. To reduce serum cholesterol levels one may consumed diet supplementat of fermented dairy product such as dadih. Lactic acid bacteria present in dadih may alter serum cholesterol by directly bind to dietary cholesterol and/or deconjugation of bile salts. Acid and bile tolerance, deconjugation of sodium taurocholate, and the cholesterol-binding ability of lactic acid bacteria from dadih were examined. Among ten dadih lactic acid bacteria tested, six strains namely I-11, I-2775, K-5, I-6257, IS-7257, and B-4 could bind cholesterol and deconjugate sodium taurocholate. However, the last four strains were very sensitive to bile. Therefore, Lactobacillus fermentum I-11 and Leuconostoc lactis subsp. lactis I-2775 those were tolerant to acid and oxgall (bile) and deconjugated sodium taurocholate and bound cholesterol could be recommended as probiotic to prevent coronary heart disease.

Heavy Metals Bioaccumulation of Several Bradyrhizobium japonicum Strains ADE NOOR SYAMSUDIN; TEDJA -IMAS; SUMINAR SETIATI ACHMADI
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

Heavy metal utilization in industry and agriculture have caused an environmental problem to existing life. Bioaccumulation is made up by a concentration of certain chemical compounds in living tissues. The objective of this research was to reveal the ability of lipopolysaccharides (LPSs) of heavy metal Bradyrhizobium japonicum tolerant strains in accumulating heavy metals. The strains used were BDG 10, KDR 10, and KDR 15. The ability of each strains on heavy metal accumulation of Cu, Pb, Zn, Ni, and Cd were quantitatively determined using atomic absorption spectrophotometry. The result showed that each strains has its own capacity to accumulate heavy metals. Accumulation of Cu (0.100), Pb (0.320), and Cd (0.048) ppm/mg dry weight by KDR 10 seem higher than BDG 10 and KDR 15. The highest accumulation of Zn and Ni was shown by KDR 15 in which the value were 0.360 and 0.165 ppm/mg dry weight, respectively and the least accumulation of all heavy metal studied was shown by BDG 10.

In Vitro Development of Ovine Embryos Following Maturation Under Limited CO2 ENDANG TRI MARGAWATI
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

An experiment was conducted to examine the influence of CO2 during in vitro oocyte maturation on the in vitro ovine embryo development. Three treatments of CO2 were subjected to the oocyte development. Those were 2h gasses prior to maturation in incubator (T1); without CO2 either prior to or over maturation (T2) and CO2 exposure both prior to and over 22h maturation (T3). A total of 324 oocytes were used. Putative zygotes were cultured for seven days and evaluated for their developmental stage. Presence of CO2 (T3) increased the proportion of oocytes reaching Metaphase II ( 66.50 + 3.5%; p

Yield Potential of Ten Peanut Introgression Lines derived from Crosses between Arachis cardenassii and A. hypogaea AHMAD RIDUAN; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

Diploid species of peanut (Arachis cardenasii) showed no symptoms of PStV infection when mechanically inoculated with PStV. Some introgression lines derived from A. cardenasii and A. hypogaea hybridization have been introduced to Indonesia. Evaluation of their adaptability and yield potential were necessary before pursuing further utilization of these introgression lines. The objectives of this research were to determine yield potential of the introgression lines of peanut in green house and field conditions and to evaluate incidence of PStV infection in the field. Peanut plants were grown in the green house and in the field according to standard procedures for raising peanut. Results of the experiments showed that growth and developmental characters of the tested lines were similar between field and green house grown plants. The introgression lines generally exhibited higher secondary branches and longer to flower and harvest as compared to peanut cv. Gajah and Kelinci. The NC-CS30 line was identfied as having higher yield and bigger seed size as compared to standard peanut cultivars (Gajah and Kelinci). Therefore, NC-CS30 germplasm may be further developed as commercial peanut cultivar or be used as donor for peanut breeding in Indonesia.

Moth Diversity at Sebangau Peat Swamp and Busang River Secondary Rain Forest, Central Kalimantan HARI SUTRISNO
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

A study on the diversity of moths was conducted from July to Augustus 2004 at the peat swamp forest Setya Alam research station, Sebangau, Central Kalimantan. The result showed that diversity of moths at this area was lower (100 species of 12 families; H = 6.643, E = 0.794) than that in secondary rain forest Busang River (278 species of 19 families; H = 8.139, E = 0.831). The result also showed that the similarity index (Cj) of the two areas was very low (0.05). Geometridae, Noctuidae, and Pyralidae were dominant in both areas. There might be more species that have not been found during eight night sampling as indicated by the species numbers in both areas has not reach a plateau.

Page 1 of 1 | Total Record : 8

Filter by Year

2005 2005

Filter By Issues

All Issue Vol. 33 No. 2 (2026): March 2026 Vol. 33 No. 1 (2026): January 2026 Vol. 32 No. 6 (2025): November 2025 Vol. 32 No. 5 (2025): September 2025 Vol. 32 No. 4 (2025): July 2025 Vol. 32 No. 3 (2025): May 2025 Vol. 32 No. 2 (2025): March 2025 Vol. 32 No. 1 (2025): January 2025 Vol. 31 No. 6 (2024): November 2024 Vol. 31 No. 5 (2024): September 2024 Vol. 31 No. 4 (2024): July 2024 Vol. 31 No. 3 (2024): May 2024 Vol. 31 No. 2 (2024): March 2024 Vol. 31 No. 1 (2024): January 2024 Vol. 30 No. 6 (2023): November 2023 Vol. 30 No. 5 (2023): September 2023 Vol. 30 No. 4 (2023): July 2023 Vol. 30 No. 3 (2023): May 2023 Vol. 30 No. 2 (2023): March 2023 Vol. 30 No. 1 (2023): January 2023 Vol. 29 No. 6 (2022): November 2022 Vol. 29 No. 5 (2022): September 2022 Vol. 29 No. 4 (2022): July 2022 Vol. 29 No. 3 (2022): May 2022 Vol. 29 No. 2 (2022): March 2022 Vol. 29 No. 1 (2022): January 2022 Vol. 28 No. 4 (2021): October 2021 Vol. 28 No. 3 (2021): July 2021 Vol. 28 No. 2 (2021): April 2021 Vol. 28 No. 1 (2021): January 2021 Vol. 27 No. 4 (2020): October 2020 Vol. 27 No. 3 (2020): July 2020 Vol. 27 No. 2 (2020): April 2020 Vol. 27 No. 1 (2020): January 2020 Vol. 26 No. 4 (2019): October 2019 Vol. 26 No. 3 (2019): July 2019 Vol. 26 No. 2 (2019): April 2019 Vol. 26 No. 1 (2019): January 2019 Vol. 25 No. 4 (2018): October 2018 Vol. 25 No. 3 (2018): July 2018 Vol. 25 No. 2 (2018): April 2018 Vol. 25 No. 1 (2018): January 2018 Vol. 24 No. 4 (2017): October 2017 Vol. 24 No. 3 (2017): July 2017 Vol. 24 No. 2 (2017): April 2017 Vol. 24 No. 1 (2017): January 2017 Vol. 23 No. 4 (2016): October 2016 Vol. 23 No. 3 (2016): July 2016 Vol. 23 No. 2 (2016): April 2016 Vol. 23 No. 1 (2016): January 2016 Vol. 22 No. 4 (2015): October 2015 Vol. 22 No. 3 (2015): July 2015 Vol. 22 No. 2 (2015): April 2015 Vol. 22 No. 1 (2015): January 2015 Vol. 21 No. 4 (2014): December 2014 Vol. 21 No. 3 (2014): September 2014 Vol. 21 No. 2 (2014): June 2014 Vol. 21 No. 1 (2014): March 2014 Vol. 20 No. 4 (2013): December 2013 Vol. 20 No. 3 (2013): September 2013 Vol. 20 No. 2 (2013): June 2013 Vol. 20 No. 1 (2013): March 2013 Vol. 19 No. 4 (2012): December 2012 Vol. 19 No. 3 (2012): September 2012 Vol. 19 No. 2 (2012): June 2012 Vol. 19 No. 1 (2012): March 2012 Vol. 18 No. 4 (2011): December 2011 Vol. 18 No. 3 (2011): September 2011 Vol. 18 No. 2 (2011): June 2011 Vol. 18 No. 1 (2011): March 2011 Vol. 17 No. 4 (2010): December 2010 Vol. 17 No. 3 (2010): September 2010 Vol. 17 No. 2 (2010): June 2010 Vol. 17 No. 1 (2010): March 2010 Vol. 16 No. 4 (2009): December 2009 Vol. 16 No. 3 (2009): September 2009 Vol. 16 No. 2 (2009): June 2009 Vol. 16 No. 1 (2009): March 2009 Vol. 15 No. 4 (2008): December 2008 Vol. 15 No. 3 (2008): September 2008 Vol. 15 No. 2 (2008): June 2008 Vol. 15 No. 1 (2008): March 2008 Vol. 14 No. 4 (2007): December 2007 Vol. 14 No. 3 (2007): September 2007 Vol. 14 No. 2 (2007): June 2007 Vol. 14 No. 1 (2007): March 2007 Vol. 13 No. 4 (2006): December 2006 Vol. 13 No. 3 (2006): September 2006 Vol. 13 No. 2 (2006): June 2006 Vol. 13 No. 1 (2006): March 2006 Vol. 12 No. 4 (2005): December 2005 Vol. 12 No. 3 (2005): September 2005 Vol. 12 No. 2 (2005): June 2005 Vol. 12 No. 1 (2005): March 2005 More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official hayati_j_biosci@cbn.net.id

Editorial Address -

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA