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TOLERANSI TEMBAKAU TRANSGENIK YANG MENGEKSPRESIKAN GEN P5CS TERHADAP STRES KEKERINGAN Riduan, Ahmad; Aswidinnoor, Hajrial; , Sudarsono; Santoso, Djoko; , Endrizal
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 13, No 2 (2010): Juli 2010
Publisher : Jurnal Pengkajian dan Pengembangan Teknologi Pertanian

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Abstract

Tolerance of Transgenic Tobacco Expressing P5CS Gene Against Drought Stress. Drought is majorosmotic stress that dramatically limit plant growth and productivity. Proline accumulation has been correlatedwith tolerance to drought stress in plants. Therefore, overproduction of proline in plants may lead to increasedtolerance against these abiotic stresses. The objectives of this experiment were to determine the effects of droughtstress at the period of 15 – 90 days after planting (DAP) on growth of T1 plants derived from transgenic GStobacco, to evaluate their tolerance against drought stress, and to determine their leaf proline content. One groupof the tobacco plants were grown in plastic pots and subjected to stress condition during the period of 15 – 90DAP. The other group was grown optimally in plastic pot up to harvest period. All tobacco plants were harvestedat 91 DAP. Leaf proline content was determined at 63 DAP (after six periods of stress). The results indicatedreduced plant height, shoot diameter, leaf number, leaf dry weight and leaf area of all tobacco plants. Stresssensitivity index calculated using leaf dry weight character grouped T1 plants derived from P5CS transgenicGS tobacco into tolerance, medium tolerance and sensitive against drought stress while that of non-transgenicGS tobacco were only medium tolerance and sensitive against drought stress. Higher leaf proline content underdrought stress was observed in all T1 plants derived from P5CS transgenic tobacco than that of non-transgenicGS tobacco. These data demonstrated that proline accumulation as an osmoprotectant and that over-expressionP5CS gene results in the increased tolerance to osmotic stress in T1 plants derived from P5CS transgenic tobacco.Key words: Proline biosynthesis, proline accumulation, sensitivity index, biomass yield Stres kekeringan merupakan masalah utama stres osmotik yang dapat menjadi faktor pembataspertumbuhan dan produktivitas tanaman. Akumulasi prolina berkorelasi dengan tingkat toleransi tanamanterhadap stres kekeringan. Oleh karena itu over-produksi prolina diduga dapat meningkatkan toleransi tanamanterhadap stres kekeringan. Tujuan penelitian ini adalah (1) untuk mengevaluasi pengaruh stres kekeringanmelalui pengurangan air terhadap pertumbuhan dan hasil tanaman TI zuriat dari tembakau GS transgenik P5CSgenerasi TO, (2) menganalisis akumulasi prolina daun tanaman TI zurlat dari tembakau GS transgenik P5CSgenerasi TO kondisi stres dan non-stres, serta (3) menganalisis hubungan antara akumulasi prolina daun padakondisi stres kekeringan dengan pertumbuhan dan hasil tanaman. Percobaan dua faktor (tembakau transgenikstres kekeringan) disusun dengan rancangan acak kelompok. Sebagian tanaman yang diuji disiram setiap hari hingga mencapai kondisi kapasitas lapang dari awal tanam sampai dengan 90 HST dan digunakan sebagaiperlakuan non-stres. Sedangkan kelompok tanaman yang lain dipelihara dalam kondisi kapasitas lapanghingga 14 HST dan diberi perlakuan stres kekeringan dari umur 15 HST hingga panen (90 HST). Kandunganprolina diukur pada umur 63 HST (setelah 6x periode stres). Hasil penelitian menunjukkan semua tanamantembakau yang diuji mempunyai tinggi tanaman, diameter batang, jumlah, berat kering dan luas daun yanglebih rendah akibat perlakuan stres kekeringan yang diberikan dibandingkan dengan kondisi non-stres.Berdasarkan hasil perhitungan indeks sensitivitas terhadap stres kekeringan menggunakan peubah bobotdaun kering per tanaman maka tanaman TI zurlat dari tembakau GS transgenik P5CS generasi TO yang diujibersegregasi untuk kategori toleran, medium toleran dan peka, sedangkan tembakau GS non-transgenikdikategorikan sebagai medium toleran dan peka terhadap stres kekeringan. Tanaman TI zurlat dari tembakau GStransgenik P5CS generasi TO menunjukkan kandungan prolina yang lebih tinggi dalam kondisi stres kekeringandibandingkan dengan tembakau GS non-transgenik. Peningkatan akumulasi prolina yang cukup tinggi akibatover-ekspresi dari gen P5CS diduga berkorelasi dengan peningkatan toleransi tanaman terhadap stres kekeringan.Kata kunci : Biosintesis prolina, akumulasi prolina, indeks sensitivitas terhadap stres, produksi biomasa
Tolerance of Several Peanut Cultivars against Drought Stress AHMAD RIDUAN; HAJRIAL ASWIDINNOOR; JAJAH KOSWARA; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 12 No. 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.019 KB) | DOI: 10.4308/hjb.12.1.28

Abstract

The objectives of this experiments were to determine the effects of drought stress initiated at vegetative growth stage on yield of peanut, to evaluate the tolerance of six peanut cultivars against drought stress, and to determine their leaf prolin and total sugar content. One group of peanut plants were grown in plastic pots and subjected to stress condition during the period of 15-45 days (Experiment I) or 15-80 days (Experiment II) by watering them only in every 4-7 days and were harvested at days 75 (Experiment I) or at maturity (Experiment II). The other group was grown optimally in plastic pot up to harvest period. Leaf prolin content was determined at 12 (before stress), 30 (after three periods of stress), 47 (after six periods of stress), and 64 days in Experiment I and only after six periods of drought stress in Experiment II. Results indicated that drought stress that was initiated at vegetative growth stage reduced plant height, branches number, shoot and root dry weight, and peanut yield. Sensitivity index calculated based on peanut yield showed that peanut cv. Jerapah and Singa were a medium tolerance while Gajah, Kelinci, Macan, and Simpai were sensitive against drought stress. Higher increased in leaf proline content after drought stress was observed in peanut cv. Jerapah and Singa, while less increased was observed in peanut cv. Gajah, Kelinci, Macan and Simpai. Total leaf sugar content of peanut cv. Jerapah, Gajah, Macan and Simpai decreased under drought stress while that of Singa and Kelinci were the same as that under optimal condition. The ability to increase leaf proline content and maintain total leaf sugar content under drought stress could be used as indicators of drought tolerance in peanut cultivars.
Yield Potential of Ten Peanut Introgression Lines derived from Crosses between Arachis cardenassii and A. hypogaea AHMAD RIDUAN; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.526 KB) | DOI: 10.4308/hjb.12.3.116

Abstract

Diploid species of peanut (Arachis cardenasii) showed no symptoms of PStV infection when mechanically inoculated with PStV. Some introgression lines derived from A. cardenasii and A. hypogaea hybridization have been introduced to Indonesia. Evaluation of their adaptability and yield potential were necessary before pursuing further utilization of these introgression lines. The objectives of this research were to determine yield potential of the introgression lines of peanut in green house and field conditions and to evaluate incidence of PStV infection in the field. Peanut plants were grown in the green house and in the field according to standard procedures for raising peanut. Results of the experiments showed that growth and developmental characters of the tested lines were similar between field and green house grown plants. The introgression lines generally exhibited higher secondary branches and longer to flower and harvest as compared to peanut cv. Gajah and Kelinci. The NC-CS30 line was identfied as having higher yield and bigger seed size as compared to standard peanut cultivars (Gajah and Kelinci). Therefore, NC-CS30 germplasm may be further developed as commercial peanut cultivar or be used as donor for peanut breeding in Indonesia.
TOLERANSI TEMBAKAU TRANSGENIK YANG MENGEKSPRESIKAN GEN P5CS TERHADAP STRES KEKERINGAN Ahmad Riduan; Hajrial Aswidinnoor; Sudarsono ;; Djoko Santoso; Endrizal ;
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 13, No 2 (2010): Juli 2010
Publisher : Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpptp.v13n2.2010.p%p

Abstract

Tolerance of Transgenic Tobacco Expressing P5CS Gene Against Drought Stress. Drought is majorosmotic stress that dramatically limit plant growth and productivity. Proline accumulation has been correlatedwith tolerance to drought stress in plants. Therefore, overproduction of proline in plants may lead to increasedtolerance against these abiotic stresses. The objectives of this experiment were to determine the effects of droughtstress at the period of 15 – 90 days after planting (DAP) on growth of T1 plants derived from transgenic GStobacco, to evaluate their tolerance against drought stress, and to determine their leaf proline content. One groupof the tobacco plants were grown in plastic pots and subjected to stress condition during the period of 15 – 90DAP. The other group was grown optimally in plastic pot up to harvest period. All tobacco plants were harvestedat 91 DAP. Leaf proline content was determined at 63 DAP (after six periods of stress). The results indicatedreduced plant height, shoot diameter, leaf number, leaf dry weight and leaf area of all tobacco plants. Stresssensitivity index calculated using leaf dry weight character grouped T1 plants derived from P5CS transgenicGS tobacco into tolerance, medium tolerance and sensitive against drought stress while that of non-transgenicGS tobacco were only medium tolerance and sensitive against drought stress. Higher leaf proline content underdrought stress was observed in all T1 plants derived from P5CS transgenic tobacco than that of non-transgenicGS tobacco. These data demonstrated that proline accumulation as an osmoprotectant and that over-expressionP5CS gene results in the increased tolerance to osmotic stress in T1 plants derived from P5CS transgenic tobacco.Key words: Proline biosynthesis, proline accumulation, sensitivity index, biomass yield Stres kekeringan merupakan masalah utama stres osmotik yang dapat menjadi faktor pembataspertumbuhan dan produktivitas tanaman. Akumulasi prolina berkorelasi dengan tingkat toleransi tanamanterhadap stres kekeringan. Oleh karena itu over-produksi prolina diduga dapat meningkatkan toleransi tanamanterhadap stres kekeringan. Tujuan penelitian ini adalah (1) untuk mengevaluasi pengaruh stres kekeringanmelalui pengurangan air terhadap pertumbuhan dan hasil tanaman TI zuriat dari tembakau GS transgenik P5CSgenerasi TO, (2) menganalisis akumulasi prolina daun tanaman TI zurlat dari tembakau GS transgenik P5CSgenerasi TO kondisi stres dan non-stres, serta (3) menganalisis hubungan antara akumulasi prolina daun padakondisi stres kekeringan dengan pertumbuhan dan hasil tanaman. Percobaan dua faktor (tembakau transgenikstres kekeringan) disusun dengan rancangan acak kelompok. Sebagian tanaman yang diuji disiram setiap hari hingga mencapai kondisi kapasitas lapang dari awal tanam sampai dengan 90 HST dan digunakan sebagaiperlakuan non-stres. Sedangkan kelompok tanaman yang lain dipelihara dalam kondisi kapasitas lapanghingga 14 HST dan diberi perlakuan stres kekeringan dari umur 15 HST hingga panen (90 HST). Kandunganprolina diukur pada umur 63 HST (setelah 6x periode stres). Hasil penelitian menunjukkan semua tanamantembakau yang diuji mempunyai tinggi tanaman, diameter batang, jumlah, berat kering dan luas daun yanglebih rendah akibat perlakuan stres kekeringan yang diberikan dibandingkan dengan kondisi non-stres.Berdasarkan hasil perhitungan indeks sensitivitas terhadap stres kekeringan menggunakan peubah bobotdaun kering per tanaman maka tanaman TI zurlat dari tembakau GS transgenik P5CS generasi TO yang diujibersegregasi untuk kategori toleran, medium toleran dan peka, sedangkan tembakau GS non-transgenikdikategorikan sebagai medium toleran dan peka terhadap stres kekeringan. Tanaman TI zurlat dari tembakau GStransgenik P5CS generasi TO menunjukkan kandungan prolina yang lebih tinggi dalam kondisi stres kekeringandibandingkan dengan tembakau GS non-transgenik. Peningkatan akumulasi prolina yang cukup tinggi akibatover-ekspresi dari gen P5CS diduga berkorelasi dengan peningkatan toleransi tanaman terhadap stres kekeringan.Kata kunci : Biosintesis prolina, akumulasi prolina, indeks sensitivitas terhadap stres, produksi biomasa
Potensi Penggunaan Abu Terbang Cangkang Sawit sebagai Adsorben Logam Arsen dari Air Limbah Tambang Rainiyati Rainiyati; Ahmad Riduan; Sarah Fiebrina Heraningsih
Jurnal Civronlit Unbari Vol 5, No 2 (2020): Oktober
Publisher : Universitas Batanghari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/civronlit.v5i2.66

Abstract

Arsenic is a dangerous compound that accumulates in nature as a result of anthropogenic activities, one of which is gold mining. Effluent originating from the remaining mining is simply abandoned even though it contains Arsenic which is above the threshold set by the Government of the Republic of Indonesia. The purpose of this study was to analyze the ability of palm shell fly ash to remove arsenic in the residual gold mining effluent. The technology applied in this study was absorption by direct mixing followed by settling and filtering. The maximum arsenic removal efficiency achieved in this study was valued at 81.98%. The longer the contact time between the palm shell fly ash and the waste sample, the less the amount of Arsenic in the waste water will be. The results of this study indicate that the method of removing arsenic from wastewater using palm shell fly ash is very effective, easy to apply and cheap in terms of the cost required.
Mercury removal from gold mining wastewater using palm oil fuel ash (POFA) Rainiyati Rainiyati; Ahmad Riduan; Sarah Fiebrina Heraningsih; Bunga Andari Mukhtasida
Journal of Degraded and Mining Lands Management Vol 9, No 3 (2022)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2022.093.3525

Abstract

Mercury is a harmful element that commonly accumulates in the environment through anthropogenic activities such as gold mining. This study aimed to examine the use of palm oil fuel ash (POFA) as an agricultural waste to remove mercury in gold mining wastewater. The technology applied in this study was adsorption and precipitation. POFA was first washed until neutral then mixed with gold mining effluent with time variation of 30, 60, 90 minutes. Next, precipitate it for 30 minutes. The adsorbent dose used in this study was 250 mg with 1 litre effluent. The maximum adsorption efficiency observed in this study was 96.77%. The utilization of POFA as an adsorbent reduced mercury levels in wastewater below the allowed released mercury level to the environment (0.0025 mg Hg/L). Results indicate that reducing mercury from gold mining wastewater by utilizing POFA was simple, effective, and low-cost to be implemented.
PENGARUH PEMBERIAN MULSA PLASTIK HITAM PERAK (MPHP) TERHADAP PERTUMBUHAN DAN HASIL DUA VARIETAS TANAMAN CABAI (Capsicum annuum L.) Erni Yulia; Ahmad Riduan; Tiur Hermawati
Bioplantae Vol. 1 No. 01 (2015): Bioplantae
Publisher : Bioplantae

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.861 KB)

Abstract

Chili is a vegetable that has high economic value, but to increase production is hampered by high attacks of pests and diseases. One effort to improve it is by using mulch and selecting superior varieties of chili plants. The study aimed to examine the effect of Black Silver Plastic Mulch (MPHP) on the growth and yield of two chili varieties (Capsicum annuum L). The study used a Randomized Block Design (RBD) with one factor of combination of variety and type of mulch.. The combination of two varieties and mulching is as follows: P1 = (Mario variety + without MPHP), P2 = (Jatilaba variety + without MPHP), P3 = (Mario + MPHP variety), P4 = (Jatilaba + MPHP variety). To see the effect of treatment, the data were processed statistically by analysis of variance, and for further tests using the Smallest Significant Difference Test (LSD) at the level of α = 5%. The combination of the use of MPHP and two chili varieties affected plant height, number of fruit crops, yield of 2X yields, and weight vanished. The use of Mario varieties given MPHP can increase yields on variable height of chili plants, number of fruit crops, yields and weight loss. Chili Mario varieties more resistant than Jatilaba using MPHP .
Resistensi 10 Galur Kacang Tanah Hasil Silangan antara Arachis cardenassii dan A. hypogaea terhadap Infeksi Peanut stripe virus (PStV) Ahmad Riduan; Sudarsono Sudarsono
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12117

Abstract

One of the pathogen infecting peanut in Indonesia is Peanut stripe virus (PStV,), causing stripe and blotch symptoms on infected peanut leaves. The objectives of this research were to evaluate effects of PStV infection on yield of 10 introgression lines of peanut derived from crossing of Arachis cardenasii and A. hypogaea, and to determine the tolerance of these lines against PStV infection. Peanut plants were grown in polybag containing 10 kg of potting soils and were grown under glasshouse conditions. The experimental unit consisted of four plants grown separately in four containers and for each treatment was replicated four times. Peanut plants were inoculated mechanically with Bogor isolate of PStV at 15 days after planting (dap) and harvested at 95-100 dap. Results of the experiment indicated peanut cv. Gajah belonged to moderate tolerance while Kelinci was sensitive against PStV infection. Introgression line NC-CS11, CS30 and WS4 were grouped as tolerance while NC-CS51, WSl, and WS3 were moderate tolerance. The tolerance lines showed mild mosaic symptoms, did not show reduction of plant height and peanut yield upon inoculation with PStV. Introgresion line NC-CS15, CS20,CS22, and CS50 were sensitive against PStV infection, showed moderate to severe mosaic/blotch symptoms, reduction of plant height and peanut yield due to PStV infection. Among the tolerance and moderate tolerance lines, only NC-CS30 showed higher yield as compared to peanut cv. Gajah or Kelinci. Therefore, this line may be developed further as commercial peanut cultivar or be use as donor germplasm for PStV tolerance mechanisms in peanut breeding.
Produksi Air Bersih di Ma’had Rizqullah Desa Simpang Sungai Duren Kecamatan Jambi Luar Kota Muara Jambi Ahmad Riduan; Rainiyati Rainiyati; Sarah Fiebrina Heraningsih; Dian Ariansyah
Jurnal Pengabdian Pada Masyarakat Vol 4 No 4 (2019)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1082.245 KB) | DOI: 10.30653/002.201944.225

Abstract

CLEAN WATER PRODUCTION IN RIZQULLAH ISLAMIC BOARDING SCHOOL JAMBI. Pondok Pesantren is an Islamic boarding school where students are learning about Islam as well as science and technologies. Adequate and good quality of facilities and infrastructures are needed to support good teaching and learning process. One of such facilities is clean and healthy water which is important to students and teachers in the Pondok Pesantren. Bad quality of water, either for washing or drinking, will affect students’ concentration in studying. Therefore, our community service project was aimed at improving the quality of water at Ma’had Rizqullah by installing a clean water system using multilevel filtration method. The water to be processed was ground water collected from bore wells in Pondok area. By this technology, the requirement of clean water in Ma’had Rizqullah can be met, al can also supply water needs for community around the Pondok. The product of this technology was clean water that can be used for daily activities by students and teachers living in Pondok and surrounding people.
Pemanfaatan Sampah Rumah Tangga Menjadi Beberapa Jenis Pupuk Cair MOL (Mikro Organisme Lokal) di Desa Pudak Kecamatan Kumpeh Ulu Kabupaten Muara Jambi Rainiyati Rainiyati; Ahmad Riduan; Zulkarnain Zulkarnain; Eliyanti Eliyanti; Sarah Fiebrina Heraningsih
Jurnal Pengabdian Pada Masyarakat Vol 4 No 4 (2019)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.324 KB) | DOI: 10.30653/002.201944.227

Abstract

THE PROCESSING OF HOUSEHOLD WASTE INTO VARIOUS TYPES OF LOCAL MICRO ORGANISMS (LMO) LIQUID FERTILIZERS IN PUDAK VILLAGE, KUMPEH ULU DISTRICT, MUARO JAMBI REGENCY. Household waste such as leftover rice and vegetables can be used as a major component in producing Local Micro Organism (LMO). Local Micro Organism has many advantages, including being used as liquid organic fertilizer, as a decomposer in compost processing, as well as being used as bio-pesticides. The purpose of this community service project was to help the people, particularly farmers, in Pudak Village in producing LMO liquid fertilizer by providing them with relevant knowledge and technology. The technology was developed by utilizing of leftover rice and vegetables which are normally treated as unwanted household wastes. This LMO liquid fertilizer is very much needed by farmers to increase their farm output since it was proven to be able to improve plant growth and development, which in turn increase farm production eventually. In addition, this liquid fertilizer can also be sold and resulting in additional income for farmer household, and village community in general. Finally, as is stated in the objective, this community service program has produced output in the form of LMO liquid fertilizer, which then can be applied on farmers’ field.
Co-Authors Adriani , Adriani Anggraini , Puspita Ais Anis Tatik Mariyani Antony Djong, Vanness Jan Ho Ardiyaningsih Puji Lestari Arifin Aryunis Aryunis Azhary, Rizky Badariah Badariah Badariah Badariah Baehaqi , Luqman Bimaruci Hazrati Havidz, Hazimi Bukri, Bukri Bunga Andari Mukhtasida Chandra , Muhammad Chandra, Muhammad DENI SETIAWAN Dian Ariansyah Djoko Santoso Djoko Santoso Djong, Vanness Jan Ho Antony Dody Perwira E. Lisangan, Christ Elis Kartika Elis Pebtianti Eliyanti Eliyanti Eliyanti Eliyanti Elly Indra Swari Endrizal Endrizal ; Erni Yulia Fajar, Hafiz Abdullah Nor Fibriyono, M. Wisnu Fiebrina Heraningsih, Sarah Fitria Fitria Fitriansyah HAJRIAL ASWIDINNOOR Hamdi, H Hamida Syaputri, Kharin Hariestya Viareco Huda , Ali Anhar Syi’bul Hutagaol, Samsul Bahri Hutapea, Hildasari Ichwan, Budiyanti Ida Suryani Irianto Irianto JAJAH KOSWARA Jasminarni Juhji, Juhji Junaedi Junaedi Khoirunnisa Muslimawati Liani, Dira Afrina M. Rizhan Ridha Mapegau Mapegau, Mapegau MARDIANA Marhaeni Fajar Kurniawati Mariana Mariana Mariana Marpaung, Al- Amin Hidayat Misnawati Mualimin , M Muhammad Fuad Sya’ban Muhammad Nasir Muhammad Rizhan Ridha Muthia, Okta Muzahid Akbar Hayat Nissa Sukmawati Novianti, Trie Rezky Nurhidayah, Selvi Odi Andanu Okta Muthia Sari Patricia Lubis, Patricia Puji Setyowati, Erni Dwi Rainiyati Rainiyati Rainiyati, Rainiyati Rakha Satya Idsan Ratna Yulinda Riski Dwimalida Putri Sarah Fiebrina Heraningsih Setiady, Irsyandi Syifa Sosiawan Nusifera Sudarsono Sudarsono Sudarsono Supriadi Syaepudin, Syaepudin Syahid , Abdul Syaputri, Kharin Hamida Tiur Hermawati Trias Novita Widuri, Elva Shanty Yesi Oktavia Yuia Alia Yulia Alia Yulistian, Saldi Zainap Hartati, Zainap Zakiah, Raudatul Zulkarnain Zulkarnain Zulkarnain, Zukkarnain