cover
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
hayati_j_biosci@cbn.net.id
Editorial Address
-
Location
Kota bogor,
Jawa barat
INDONESIA
HAYATI Journal of Biosciences
ISSN : 19783019     EISSN : 20864094     DOI : -
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 8 Documents
Search results for , issue "Vol. 13 No. 4 (2006): December 2006" : 8 Documents clear
Maturation Rate of Ovine Oocytes from Different Reproductive Status and Maturation Medium ARIEF BOEDIONO; YULNAWATI YULNAWATI; MOHAMAD AGUS SETIADI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.228 KB) | DOI: 10.4308/hjb.13.4.131

Abstract

The aim of the present study was to determine the number of follicles, oocyte quality and maturation rate of oocytes from pairs of ovary with different reproductive status in two maturation medium, TCM-199 as control and CR1aa as treatment. The pairs of ovary were classified into four groups: (i) ovaries with corpus luteum (CL) and dominant follicle (DF), (ii) ovaries with CL, without DF, (iii) ovaries with DF, without CL, (iv) ovaries without both CL and DF. Results of the experiment revealed that the greatest number of follicles was observed from ovary with CL without DF (15.88 + 10.68), although not significantly different (P > 0.05) with other status of ovaries. The lowest number (P < 0.05) of A grade oocytes was found from ovary with DF without CL (1.20 + 1.10). The percentage of Metaphase II was highest in TCM-199 (75.51%) with oocytes from ovaries with CL and DF, and the lowest with oocytes from ovaries with DF without CL in TCM-199 and CR1aa (42.86 and 30.95%). The study suggested that the number of oocytes with A grade were influenced by the reproductive status of ovaries. The maturation rate of A grade oocytes was influenced by the quality of oocytes and the composition of maturation medium. Key words: reproductive status, corpus luteum, dominant follicles, TCM-199, CR1aa
Spatial Analysis of Agricultural Landscape and Hymenoptera Biodiversity at Cianjur Watershed YAHER WANDI; SYAFRIDA MANUWOTO; DAMAYANTI BUCHORI; PURNAMA HIDAYAT; LILIK BUDIPRASETYO
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.536 KB) | DOI: 10.4308/hjb.13.4.137

Abstract

Hymenoptera is one of the four largest insect order (the other three are Coleoptera, Diptera, and Lepidoptera). There are curerently over 115 000 described Hymenoptera species. It is clear that Hymenoptera is one of the major components of insect biodiversity. However, Hymenoptera biodiversity is affected by ecology, environment, and ecosystem management. In an agricultural areas, the spatial structure, habitat diversity, and habitat composition may vary from cleared landscapes to structurally rich landscape. Thus, it is very likely that such large-scale spatial patterns (landscape effects) may influence local biodiversity and ecological functions. Therefore, the objective of this research were to study diversity and configuration elements of agricultural landscapes at Cianjur Watershed with geographical information sytems (GIS) and its influence on Hymenoptera biodiversity. The structural differences between agricultural landscapes of Nyalindung, Gasol, and Selajambe were characterized by patch analyst with ArcView 3.2 of digital land use data. Results indicated that class of land uses of Cianjur Watershed landscape were housing, mixed gardens, talun and rice, vegetable, and corn fields. Landscape structure influenced the biodiversity of Hymenoptera. Species richness and the species diversity were higher in Nyalindung landscape compare to Gasol and Selajambe landscape. Key words: diversity, Hymenoptera, landscape, watershed, GIS
Evolution of a Wingless Gene and its Utility for Inferring the Relationships within Glyphodes Moths HARI SUTRISNO
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.411 KB) | DOI: 10.4308/hjb.13.4.145

Abstract

The evolution of a nuclear Wingless gene was investigated and its utility for inferring the phylogenetic relationship within Glyphodes moths was assessed by comparing with other three genes namely, COI, COII, and EF-1?. The results show that the bias of base compositions in Wingless (C: 0.19) is the lowest among those in COI, COII, and EF-1?. The averages of nucleotide sequence divergences for comparison between groups based on the Wingless were the highest. While substitutions in Wingless and EF-1? genes were not saturated at the divergence of the species groups, COI and COII genes attained saturation at those levels. The phylogenetic analysis based on Wingless solely show that this gene was very useful to resolve the relationships between groups but gave a poor resolution at the specific level, i.e. the relationships within group 1 was not resolved. Combination of all data supports the phylogenetic hypothesis based on morphological data. Glyphodes falls into three species groups: group 2 branched off first then followed by groups 1 and 3. Key words: COI, COII, EF-1?, genes, Glyphodes, mitochondria, moth, phylogeny, relationships, Wingless
Characterization of Xylanase Streptomyces spp. SKK1-8 ANJA MERYANDINI; TRIO HENDARWIN; DEDEN SAPRUDIN; YULIN LESTARI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.007 KB) | DOI: 10.4308/hjb.13.4.151

Abstract

Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 0C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 0C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 0C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan. Key words: xilanase, Streptomyces spp., characterization, zymogram and SDS-PAGE, stability
Fecundity, Longevity, and Host Finding of Three Parasitoid Species of Liriomyza sativae SITI HERLINDA; AGUSMAN JAYA; YULIA PUJIASTUTI; AUNU RAUF
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.692 KB) | DOI: 10.4308/hjb.13.4.156

Abstract

Liriomyza sativae is a polyphagous agromyzid leafminer and it has invaded large part of world, i.e Hemiptarsenus varicornis, Gronotoma micromorpha, and Opius dissitus. This research were conducted to investigate progeny, immature development period, longevity of female parasitoids of L. sativae, and to study female parasitoid behaviour in host finding. Host finding behaviour was observed by examining their visit frequency to the leaves that mined by leafminer larvae and healthy leaves. Results showed that H. varicornis produced more progenies (10.70 + 2.58 progenies/female), but not significantly different (P < 0.05) from progenies produced by G. micromorpha (9.90 + 3.81 progenies/female) and O. dissitus (9.60 + 3.31 progenies/female). The immature development period of G. micromorpha (25.65 + 0.38 days) was found to be longer than H. varicornis (16.14 ± 1.20 days) and O. dissitus (14.03 + 0.22 days). Significant different (P = 0.1014) of adult longevity was not found among H. varicornis (9.22 + 2.48 days), G. micromorpha (7.25 + 1.34 days), and O. dissitus (8.74 + 2.18 days). Our analyses also indicated that G. micromorpha and O. dissitus found their hosts based on the larvae mining, however, H. varicornis performed it randomly. Based on the number of progeny and longevity of adult female, all parasitoids tested may have a potential as biological control agents of leafminer, L. sativae. Key words: fecundity, longevity, behavior, Hemiptarsenus, Gronotoma, Opius, Liriomyza sativae
Production Pattern of Ajmalicine in Catharanthus roseus (L.) G. Don. Cell Aggregates Culture in the Airlift Bioreactor RIZKITA RACHMI ESYANTI; AIDA MUSPIAH
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.025 KB) | DOI: 10.4308/hjb.13.4.161

Abstract

A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 x 10-6 M naphthalene acetic acid (NAA) and 10-5 M benzyl amino purine (BAP). Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.25 l min-1 and 0.34 l min-1, respectively. The pattern of ajmalicine production in bioreactor were observed in every three days within 24 days. Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7A Plus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.34 l min-1 aeration compare to 20 g/fw - 0.25 l min-1, 20 g/fw - 0.34 l min-1, as well as 30 g/fw – 0.25 l min-1/sup>. The highest ajmalicine content in cell aggregates was obtained on the 12 days (79.23 µg g-) whilst in medium was obtained in the 18thsup> days (981.15 µg l-). Key words: ajmalicine, cell aggregates culture, C. roseus, airlift bioreactor
Oxidative Burst, Peroxidase Activity, and Lignin Content of Sclerotium rolfsii Infected Peanut Tissue ENDANG PUDJIHARTATI; SATRIYAS ILYAS; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.142 KB) | DOI: 10.4308/hjb.13.4.166

Abstract

The objectives of this experiment were to analyse physiological responses, such as oxidative burst reaction, peroxidase activity, and lignin content of healthy and S. rolfsii-infected peanut tissues. Differences in physiological responses among 24 peanut genotypes were determined, the disease severity was calculated and used to group resistance of tested genotypes. The regressions among observed peroxidase activity, lignin content and disease severity were used to determine the possible mechanisms of S. rolfsii resistance in peanut. Peanut seeds were grown in polybag and the growing plants were inoculated at the crown, stem, and leaf tissues. Results of the experiment indicated that infection of S. rolfsii in peanut did not induce oxidative burst. However, infection of the pathogen resulted in increased peroxidase activity and lignin content in the infected tissues. Regression analysis between peroxidase activity and disease severity showed negative slopes, indicating the more resistance the genotype, the more peroxidase activity in the tissue. Regression analysis between lignin content and disease severity was not significant. Key words: hypersensitive response (HR), resistance mechanisms, Sclerotium stem rot, disease response, Arachis hypogaea
Immuncontraception Reversibility Test of Goat Zona Pellucida-3 (gZP3) on Mice (Mus musculus) IMAM MUSTOFA
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.118 KB) | DOI: 10.4308/hjb.13.4.173

Abstract

The aim of this study was to prove the reversibility of gZP3 protein on mice as an animal model. Treatment group of mice (Mus musculus) were immunized with 40 ug goat zona pellucida-3 (gZP3) as an anti fertility agent. Control group of mice were injected only with physiologic solution. The mice were injected three times, the first injection as an immunization. The second and third injected as a booster with 14 days interval. The first injection, gZP3 suspension was diluted in complete Freund’s adjuvant (CFA). The boosters, gZP3 suspension was diluted in incomplete Freund’s adjuvant (IFA) 1:1 (v/v). Blood samples were collected before immunization, seven days after the second booster and at the time of parturition. Seven days after the last injection, the mice were mated, and the parturition was observed after that. The result showed that antibody titer of immunized mice serum was increasing (P < 0.05) seven days after immunization, and decreasing (P > 0.05) after parturition compared to antibody titer of serum before immunization. Dot blotting analysis showed that gZP3 protein could recognize gZP3 antibody of the immunized mice. Protein of gZP3 as an immunocontraceptive substance was reversible in 91.60 + 4.90 days. This reversibility was longer (P < 0.05) compared to control mice i.e. 26.50 + 4.30 days. Key words: immunocontraception, goat zona pellucida-3, reversibility, mice, animal model, gZP3 antibody

Page 1 of 1 | Total Record : 8


Filter by Year

2006 2006


Filter By Issues
All Issue Vol. 33 No. 3 (2026): May 2026 Vol. 33 No. 2 (2026): March 2026 Vol. 33 No. 1 (2026): January 2026 Vol. 32 No. 6 (2025): November 2025 Vol. 32 No. 5 (2025): September 2025 Vol. 32 No. 4 (2025): July 2025 Vol. 32 No. 3 (2025): May 2025 Vol. 32 No. 2 (2025): March 2025 Vol. 32 No. 1 (2025): January 2025 Vol. 31 No. 6 (2024): November 2024 Vol. 31 No. 5 (2024): September 2024 Vol. 31 No. 4 (2024): July 2024 Vol. 31 No. 3 (2024): May 2024 Vol. 31 No. 2 (2024): March 2024 Vol. 31 No. 1 (2024): January 2024 Vol. 30 No. 6 (2023): November 2023 Vol. 30 No. 5 (2023): September 2023 Vol. 30 No. 4 (2023): July 2023 Vol. 30 No. 3 (2023): May 2023 Vol. 30 No. 2 (2023): March 2023 Vol. 30 No. 1 (2023): January 2023 Vol. 29 No. 6 (2022): November 2022 Vol. 29 No. 5 (2022): September 2022 Vol. 29 No. 4 (2022): July 2022 Vol. 29 No. 3 (2022): May 2022 Vol. 29 No. 2 (2022): March 2022 Vol. 29 No. 1 (2022): January 2022 Vol. 28 No. 4 (2021): October 2021 Vol. 28 No. 3 (2021): July 2021 Vol. 28 No. 2 (2021): April 2021 Vol. 28 No. 1 (2021): January 2021 Vol. 27 No. 4 (2020): October 2020 Vol. 27 No. 3 (2020): July 2020 Vol. 27 No. 2 (2020): April 2020 Vol. 27 No. 1 (2020): January 2020 Vol. 26 No. 4 (2019): October 2019 Vol. 26 No. 3 (2019): July 2019 Vol. 26 No. 2 (2019): April 2019 Vol. 26 No. 1 (2019): January 2019 Vol. 25 No. 4 (2018): October 2018 Vol. 25 No. 3 (2018): July 2018 Vol. 25 No. 2 (2018): April 2018 Vol. 25 No. 1 (2018): January 2018 Vol. 24 No. 4 (2017): October 2017 Vol. 24 No. 3 (2017): July 2017 Vol. 24 No. 2 (2017): April 2017 Vol. 24 No. 1 (2017): January 2017 Vol. 23 No. 4 (2016): October 2016 Vol. 23 No. 3 (2016): July 2016 Vol. 23 No. 2 (2016): April 2016 Vol. 23 No. 1 (2016): January 2016 Vol. 22 No. 4 (2015): October 2015 Vol. 22 No. 3 (2015): July 2015 Vol. 22 No. 2 (2015): April 2015 Vol. 22 No. 1 (2015): January 2015 Vol. 21 No. 4 (2014): December 2014 Vol. 21 No. 3 (2014): September 2014 Vol. 21 No. 2 (2014): June 2014 Vol. 21 No. 1 (2014): March 2014 Vol. 20 No. 4 (2013): December 2013 Vol. 20 No. 3 (2013): September 2013 Vol. 20 No. 2 (2013): June 2013 Vol. 20 No. 1 (2013): March 2013 Vol. 19 No. 4 (2012): December 2012 Vol. 19 No. 3 (2012): September 2012 Vol. 19 No. 2 (2012): June 2012 Vol. 19 No. 1 (2012): March 2012 Vol. 18 No. 4 (2011): December 2011 Vol. 18 No. 3 (2011): September 2011 Vol. 18 No. 2 (2011): June 2011 Vol. 18 No. 1 (2011): March 2011 Vol. 17 No. 4 (2010): December 2010 Vol. 17 No. 3 (2010): September 2010 Vol. 17 No. 2 (2010): June 2010 Vol. 17 No. 1 (2010): March 2010 Vol. 16 No. 4 (2009): December 2009 Vol. 16 No. 3 (2009): September 2009 Vol. 16 No. 2 (2009): June 2009 Vol. 16 No. 1 (2009): March 2009 Vol. 15 No. 4 (2008): December 2008 Vol. 15 No. 3 (2008): September 2008 Vol. 15 No. 2 (2008): June 2008 Vol. 15 No. 1 (2008): March 2008 Vol. 14 No. 4 (2007): December 2007 Vol. 14 No. 3 (2007): September 2007 Vol. 14 No. 2 (2007): June 2007 Vol. 14 No. 1 (2007): March 2007 Vol. 13 No. 4 (2006): December 2006 Vol. 13 No. 3 (2006): September 2006 Vol. 13 No. 2 (2006): June 2006 Vol. 13 No. 1 (2006): March 2006 Vol. 12 No. 4 (2005): December 2005 Vol. 12 No. 3 (2005): September 2005 Vol. 12 No. 2 (2005): June 2005 Vol. 12 No. 1 (2005): March 2005 More Issue