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MEDIA PETERNAKAN - Journal of Animal Science and Technology

mediapeternakan
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Published by Institut Pertanian Bogor

ISSN : - EISSN : - DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Arjuna Subject : -

Articles 12 Documents

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Search results for , issue "Vol. 36 No. 3 (2013): Media Peternakan" : 12 Documents clear

Intensification of Wild Silkworm Attacus atlas Rearing (Lepidoptera: Saturniidae) B K Desmawita; A M Fuah; D R Ekastuti
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Study on intensification program through reproduction and feeding management was conducted for six months to evaluate the growth and reproductive performance of wild silkworm, Attacus atlas. The experiment was divided into two phases: 1) the first phase was conducted on mating management, by applying different times of mating duration of 3, 6, 12, and 24 h as treatments to assess eggs production. In this phase, a completely randomized design was used, with 4 treatments and 5 replications; 2) the second phase was on feeding management, a completely randomized design method (2 x 2) was used, including feed (guava and walnut leaves) as first factor, and the second factor was feeding times (3 and 4 times/day) with 5 replications. The results obtained from the first experiment showed that different duration of mating significantly (P<0.05) affected the number of eggs produced. Based on time efficiency 6 hours mating duration produced higher egg production (226 egg/cycle), as well as hatchability (95%) than those of other mating duration. In the second experiment, it was found that there was interaction between feed types and feeding frequencies which significantly (P<0.05) affected feed consumption, feed digestibility, body weight gain, diameter of larvae and mortality rate. In conclusion, the optimum and efficient duration sugested for mating is six hours resulting high egg production. The walnut leaves feed has better effect on the first and second instar of larvae, while the guava leaves affect the following instars. In terms of production, four times of feeding frequency per day has higher productivity and is recommended in the cultivation management of A. atlas.

Genetic Variation of mtDNA Cytochrome Oxidase Subunit I (COI) in Local Swamp Buffaloes in Indonesia F Saputra; . Jakaria; C Sumantri
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this research was to identify genetic variation of mitochondria DNA especially in cytochrome oxidase subunit I (COI) among population of Indonesian buffaloes. Samples of swamp buffaloes were collected from Aceh (n= 3), North Sumatra (n= 3), Riau (n= 3), Banten (n= 3), Central Java (n= 3), West Nusa Tenggara (n= 3) and South Sulawesi (n= 3), and riverine buffalo from North Sumatra (n= 1) out of group for comparison. Sequence of COI was analyzed using MEGA 5.10 software with neighbor-joining method kimura 2-parameter model to reconstruct phylogeny tree. The result showed that three haplotypes for swamp buffalo and one haplotype for riverine buffalo in Indonesia resulted from 41 polymorphic sites. This finding showed that the COI gene could be considered as a marker to distinguish among swamp buffaloes in Indonesia.

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene . Irine; H Nuraini; C Sumantri
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Adulteration of animal food products for economic reason has happened during the last decades. Species identification method development was needed to prevent falsification information. The objective of this research was to study species authentication (dog, cat, and tiger) to ensure animal origin in products using cyt β gene specific marker. DNA extraction and fragment amplification were conducted using phenol-chloroform and multiplex PCR (Polymerase Chain Reaction) method, respectively. This research showed that fragment length of amplification for species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively. Species specificity was also indicated by high reverse primers homology percentage. Multiplex PCR technique succeed to amplify DNA fragment from species tested, but has a limitation to amplify total DNA composite of mix DNA.

The Relationship between Sperm Morphometry and Sperm Competition in Local Goats of Central Java, Indonesia S B Wibowo; E T Setiatin; E Kurnianto
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this study was to examine the relationship between sperm morphometry and sperm competition in local goats. Forty bucks were used in this study, consisted of 4 breeds namely Ettawa Grade Goat (EGG), Kejobong Goat (KJG), Kacang Goat (KCG), and Jawarandu Goat (JWG), each breeds consisted of 10 bucks. GLM, REG and DISRCRIM procedures of SAS were used to compute all data observed. UPGMA of MEGA 5 was used to illustrate the genetic distance among breeds. The result showed that there were differences in the sperm morphometry among 4 breeds of goats (P<0.05). Sperm length positively related to the level of sperm competition in all local goats (P<0.05) although the value of coefficient of determinant was low (r2= 0.0043). Within breed analysis showed that there was no positive relationship between sperm length and level of sperm competition in EGG and JWG goats. Midpiece area positively related to the principal piece (P<0.05). Sperm morphometry had a significant positive relation to sperm competition in local goats of Central Java.

GHRH|HaeIII Gene Polymorphism in Dairy and Beef Cattle at National Livestock Breeding Centers A O Rini; C Sumantri; A Anggraini
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was aimed to identify polymorphism of growth hormone releasing hormone (GHRH) gene in 89 heads of Holstein-Friesian (HF) dairy cattle from Lembang Artificial Insemination Center/LAIC (17 bulls), Singosari Artificial Insemination Center/SAIC (32 bulls), and Cipelang Livestock Embryo Center/CLEC (40 cows); as well as in 4 breeds of female beef cattle from CLEC for comparison, providing Simmental (13 cows), Limousin (14 cows), Brahman (5 cows), and Angus (5 cows). This study used PCR-RFLP method by using HaeIII restriction enzyme. The enzyme cut the GHRH gene at nucleotides of GG|CC at the base positions of 118, 312, and 406 and produced 4 fragments of 118, 194, 94, and 45 bp respectively. Genotyping the GHRH gene produced two types of allele, namely A (312, 94, and 45 bp) and B (194, 118, 94, and 45 bp). These two alleles resulted in three types of genotype, namely AA (312, 94, and 45 bp), AB (312, 194, 118, 94, and 45 bp), and BB (194, 118, 94, and 45 bp). Frequency of the B allele was dominant to the A allele. Chi-Square analysis showed that all of HF dairy and beef cattle observed were in Hardy-Weinberg equilibrium (X2 < X2(0.05)). The highest heterozygosity value was 0.471 for HF bulls in LAIC, while the lowest one was for HF bulls in SAIC. Heterozygosity values in Simmental and Limousin cattles were higher than that of in HF cattle. The GHRH gene in HF and beef cattle was polymorphic, the exception was for Brahman with the only B allele. This result will improve the understanding of the polymorphism of GHRH gene in dairy and beef cattle.

Characterization of Bacterial Mannanase for Hydrolyzing Palm Kernel Cake to Produce Manno-oligosaccharides Prebiotics W Utami; A Meryandini; K G Wiryawan
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Palm kernel cake (PKC) is a promising source of prebiotics, since it contains high amount of β-mannan which can be further hydrolyzed to manno-oligasaccharides (MOS), a prebiotic. Therefore, this research was carried out to analyze the capability of a bacterial isolate (A2 isolates) previously isolated from soils sample from around IPB campus to hydrolyze PKC. Based on 16S-DNA analysis, isolate A2 was identified as Brevibacillus borstelensis. Mannanase of A2 isolate had an optimum condition at 90 oC and pH 7. Mannanase activity of crude extracts using Locust Bean Gum (LBG) and PKC as substrates were 0.37U/mL and 0.032U/mL, respectively. However, the most favorable production of oligosaccharides based on the degree of polymerization was obtained after 72-h of incubation with the ratio of substrate:enzyme, 1.2:1, on 1.5% PKC as substrate. The manno-oligosaccharides prebio-tic obtained was found to interfere the growth of both lactic acid bacteria (Lactobacillus casei) and pathogenic microflora (Escherichia coli). E. coli apparently could not use this prebiotic as the carbon sources, in contrast to L. casei. Substitution of carbon source in medium with prebiotics reduced the capability of L. casei to produce organic acids. It is concluded that local A2 isolate (B. borstelensis) produces mannanase which can be used to produce prebiotics from PKC.

Egg Production and Quality of Magelang Duck Fed Diets Containing Different Ratio of Omega 3 : Omega 6 and Organic Zn A Darmawan; K G Wiryawan; . Sumiati
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aims of this study were to determine the feeding effect of diets containing different ratio of ω-3 and ω-6 fatty acids and organic Zn on egg production and quality of Magelang duck. A total of 90 ducks of 20 weeks old were randomly divided into 18 experimental units by assigning a completely randomized design with 6 treatments and 3 replications. There were 6 dietary treatments, namely 1) ration without palm oil, fish oil and organic Zn with the ratio of ω-3 : ω-6 was 1: 5 (R0), 2) ration with the ratio of ω-3 : ω-6 was 1: 1.5 + 200 ppm organic Zn (R1), 3) ration with the ratio of ω-3 : ω-6 was 1: 3 + 200 ppm organic Zn (R2), 4) ration with the ratio of ω-3 : ω-6 was 1: 4.5 + 200 ppm organic Zn (R3), 5) ration with the ratio of ω-3 : ω-6 was 1: 6 + 200 ppm organic Zn (R4), 6) ration with the ratio of ω-3 : ω-6 was 1 : 7.5 + 200 ppm organic Zn (R5). Observation was carried out for 9 wk. The results showed that the ration containing the ratio of ω-3 : ω-6 = 1 : 3 and organic Zn (R2) produced significantly (P<0.05) the highest egg production and the lowest egg production was resulted by the dietary treatment without oil and organic Zn. Ration containing the ratio of ω-3 : ω-6 = 1: 3 and organic Zn (R2) significantly (P<0.05) decreased feed conversion ratio to the lowest value and it was significantly different as compared to R0 and R5. It can be concluded that feeding diet containing ω-3 and ω-6 with the ratio of 1:3 + 200 ppm organic Zn improves egg production without decreasing egg quality.

Performance and Meat Quality of Local Sheep Administered with Feed Additive Containing Probiotic and Organic Mineral Complex H Herdian; A Sofyan; A A Sakti; H Juendra; M F Karimy; A E Suryani; E Damayanti; L Istiqomah
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The experiment was conducted to evaluate the effect of probiotic and organic mineral complex (POMC) administration on performance and meat quality of local sheep. In this study, 6 sheep with an average initial body weight of 12.67±0.81 kg were arranged in a completely randomized design with 2 treatments and 3 replications. The treatments were T0: basal diet (control) and T1: basal diet + POMC. The basal diet consisted of forage and concentrate (60:40) with dry matter of feed intake as much as 5% of body weight. Dose of POMC used in this study was 15 g/d/head. The POMC contained lactic acid bacteria, i.e. Lactobacillus sp. (1x108 cfu/g) and S. cereviseae incorporated with micromineral Co (2 ppm), Cu (100 ppm), Fe (2.5 ppm), I (110 ppm) and Mn (100 ppm). The experimental period lasted for 11 wk (1 wk for adaptation and 10 wk for data collection). Data were analyzed using t-Student statistical test to compare the treatments. The results showed that POMC administration did not affect the body weight gain (BWG) (7.46 kg) compared to control (7.13 kg) while concentrate consumption (26.9 kg) of POMC was lower than the control (28.6 kg). Meanwhile POMC administration did not affect the meat quality (pH, moisture, cooking loss and tenderness), whereas the meat cholesterol was lower (34.25 mg/100g) than the control (38.87 mg/100g). It is concluded that administration of probiotic lactic acid bacteria combined with organic minerals decreases concentrate consumption and thereby potentially increases the animals energy utilization efficiency. In addition, the treatment also decreases the meat cholesterol content of local sheep.

Production, Competition Indices, and Nutritive Values of Setaria splendida, Centrosema pubescens, and Clitoria ternatea in Mixed Cropping Systems in Peatland A Ali; L Abdullah; P D.M.H Karti; M A Chozin; D A Astuti
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This research was conducted to evaluate production, different competition indices and nutritive value of Setaria splendida, Centrosema pubescens, and Clitoria ternatea in monoculture and mix cropping system on peat soil land. The experiment was set up in a randomized complete block design with five treatments and three replications. The five treatments were: S. splendida sole cropping (SS), C. pubescens sole cropping (CP), C. ternatea sole cropping (CT), S. splendida and C. pubescens mix cropping (SS/CP) and S. splendida/C. ternatea mix cropping (SS/CT). The DM yield of S. splendida in mixed cropping with C. pubescens increased 43.4% and in mix cropping with C. ternatea increased 15.7% compared to sole S. splendida. The value of land equivalent ratio of SS/CP (LERSS/CP) was >1. The LERSS/CT value was <1. The crowding coefficient value of S. splendida (KSS) was higher than KCP and KCT. The total value of KSS/CP and KSS/CT were >1. The competition ratio (CR) values of S. splendida in both mix cropping were >1. The agressivity (A) values of S. splendida in both mix cropping were positive. The crude protein, NDF and ADF content of forage were not affected by mix cropping system. In conclusion, mix cropping in peatland do not affect productivity and nutritive value of S. splendida, C. pubescens, and C. ternatea. S. splendida is more effective in exploiting environmental resources when intercropped with C. pubescens compared to C. ternatea on peatland.

Screening of Lactic Acid Bacteria from Rumen Liquor and King Grass Silage as well as Their Antibacterial Activities A Sofyan; A N Aswari; T Purwoko; E Damayanti
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Probiotic is a live microbial culture which has positive effect on animal by improving the natural balance of microflora in the digestive tract. This experiment aimed to screen and identify indigenous lactic acid bacteria (LAB) from rumen liquor and king grass (Pennisetum hybrid) silage as a probiotic candidate and to evaluate their resistance in low pH, and inhibitory activities against pathogenic bacteria. The LAB isolate was characterized by a clear zone formed on MRSA medium + CaCO3 0.2% (w/v) and further identified by morphological and biochemical assays. The selected isolates were evaluated for their viability in low pH, pathogenic bacterial inhibition, and lactic acid production. The experimental arrangement was a factorial block design (4 x 2) consisted of four isolates and two levels of pH value (pH 2 and 3), each treatment in 3 equal replicates. The result showed that four isolates (two isolates from the rumen liquor of fistulated cattle and two isolates from silage) were identified as lactic acid bacteria. The four isolates showed inhibition activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus and performed viability at low pH during 2 h treatment. The highest lactic acid production was obtained from isolates Sil.3 (21.42%) and followed by CR2 (19.88%), CR1 (15.40%) and Sil.9 (15.08%). Biochemical identification by standard of analytical profile index (API) 50 CHL kit showed that the selected isolates CR1 was Lactobacillus paracasei ssp. paracasei 3 (91.5%), L. paracasei ssp. paracasei 3 (76.5%), Sil.3 was Lactobacillus brevis (95.1%), and Sil.9 was Lactobacillus collinoides (92.5%). In conclusion, probiotic candidates isolated from rumen liquor are confirmed as L. paracasei ssp. paracasei (CR1 and CR2), while two other isolates from king grass silage are identified as L. brevis (Sil.3) and L. collinoides (Sil.9). L. brevis (Sil.3) and L. paracasei ssp. paracasei (CR1) has higher inhibition against pathogenic bacteria (E. coli, S. aureus, and B. subtilis) than L. paracasei ssp. paracasei (CR2) and L. collinoides (Sil.9).

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