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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 5 Documents

Search results for , issue "Vol 13 No 4 (2017)" : 5 Documents clear

Efektivitas Formula Bacillus subtilis TM4 untuk Pengendalian Penyakit pada Tanaman Jagung Nurasiah Djaenuddin; Nurnina Nonci; Amran Muis
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Banded leaf and sheath blight (BLSB) and maydis leaf blight (MLB) caused by Rhizoctonia solani and Bipolaris maydis, respectively are considered as important diseases in maize. The use of biopesticides is an alternative method to control the diseases. This study was conducted to determine the effectiveness of bacterial formula Bacillus subtilis to inhibit the development of BLSB and MLB on the plant. Testing of biopesticide formula was done in two different applications, i.e. seed treatment for BLSB control and leaf spraying in the field for MLB. The results showed that the B.subtilis formula effectively suppressed the development of BLSB but it was not effectively suppressed the development of MLB .Key words: Bacillus subtilis, biopesticide, Bipolaris maydis, leaf blight diseaseBanded leaf and sheath blight (BLSB) and maydis leaf blight (MLB) caused by Rhizoctonia solani and Bipolaris maydis, respectively are considered as important diseases in maize. The use of biopesticides is an alternative method to control the diseases. This study was conducted to determine the effectiveness of bacterial formula Bacillus subtilis to inhibit the development of BLSB and MLB on the plant. Testing of biopesticide formula was done in two different applications, i.e. seed treatment for BLSB control and leaf spraying in the field for MLB. The results showed that the B.subtilis formula effectively suppressed the development of BLSB but it was not effectively suppressed the development of MLB.

Sensitivitas Colletotrichum spp. pada Cabai terhadap Benomil, Klorotalonil, Mankozeb, dan Propineb Desta Andriani; Suryo Wiyono; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Anthracnose caused by several species of Colletotrichum is one of limiting factors for chilli production. Up to now the control of antrachnose disease still rely on regular and intensive application of synthetic fungicides. Application of similar active ingredients of synthetic fungicides may create resistance of the pathogens. This study was conducted to evaluate the sensitivity of three species of Colletotrichum against four commercial fungicides with different active ingredients, i.e. benomyl, chlorotalonil, mancozeb, and propineb. The sensitivity level and the potential occurrence of resistance of Colletotrichum were observed based on relative inhibition rate using poisoning food method. The results showed that all isolates were highly resistant to chlorotalonil, even up to ten times of the highest recommended concentrations. The tested fungal isolates were resistant or highly resistant to the recommended concentrations of mancozeb and propineb, but they were still sensitive when the concentrations were increased up to five times. Isolates of Colletotrichum spp. showed sensitive to highly sensitive response to benomyl.

Penanda Molekuler Inter Simple Sequence Repeat untuk Menentukan Ketahanan Tanaman Jagung terhadap Penyakit Bulai Polikarpia Wilhelmina Bani; Budi Setiadi Daryono; Purnomo Purnomo
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Downy mildew disease of maize, caused by the fungus Peronosclerospora maydis, can cause serious yield loss. Molecular markers-based method is usually performed to look for the variation of resistance in plants. In this study, 5 primers of inter simple sequence repeat (ISSR) was evaluated for molecular marker to determine maize resistance to downy mildew. The average polymorphic of 5 ISSR primers was 72.84%. Primer ISSR 808 (AG) 8C was able to differentiate resistant cultivar (cv Talenta) from susceptible cultivar (cv Pulut).

Keefektifan Perlakuan Fisik dan Minyak Atsiri untuk Mengeliminasi Bakteri Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Siti Tri Wahyuni; Ali Nurmansyah; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Clavibacter michiganensis subsp. michiganensis is the causal agent of bacterial canker disease of tomato. The bacteria is seed borne and may cause yield loss up to 70%. Seed treatment is an alternative method for controlling bacterial canker. The objective of the research was to study the effectiveness of physical and essential oil treatment for elimination of C. michiganensis subsp. michiganensis from tomato seed. Research was conducted in 4 separate experiments, i.e. (1) screening essential oils to control C. michiganensis subsp. Michiganensis; (2) to determine the treatment window of physical and essential oil treatment; (3) to determine the physical and essential oil treatment on tomato seed infested by C. michiganensis subsp. Michiganensis; and (4) to determine the effectiveness of treatment combination of physical and essential oil. The results showed that betel oil at concentration of 8% had the greatest inhibitory level (approximately 27.33 mm). Hot water treatment (53 °C) for 25 minutes reduced 90.94% of C. michiganensis subsp. michiganensis population; whereas dry heat treatment (60 °C, 24 hours) and betel oil treatment at 0.25% concentration reduced C. michiganensis subsp. michiganensis population (85.13 and 99.82% respectively). The combination of betel oil (0.5%), hot water (55 °C), and dry heat treatments (60 °C) was the most effective control method, because it reduced 99.99% of C. michiganensis subsp. michiganensis population and maintained the germination level of seed up to 100%. This combination might be recommended for seed treatment to eliminate C. michiganensis subsp. michiganensis.

Identifikasi Molekuler Fitoplasma yang Berasosiasi dengan Tanaman Kaktus Hias Opuntia sp. Ariny Prasetya; Kikin Hamzah Mutaqin; Meity Suradji Sinaga; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Cactus species (Opuntia sp.) is a popular ornamental succulent plant. Some ornamental cactus species in Indonesia showing proliferation and green mosaic pattern symptoms have been reported to be associated with phytoplasma infection. However, further molecular identification for accurate classification of the causal phytoplasma has not been done. This study aimed to diagnose phytoplasma associated with Opuntia sp. based on molecular methods involving PCR standard combined with nested-PCR, cloning and DNA sequencing. Standard PCR was carried out using P1/P7 primers followed by nested-PCR using R16F2n/R16R2 or fU5/rU3 primer pairs which amplify the 16S rRNA gene targets of 1.2 kb and 880 bp, respectively. Amplified fragment of nested-PCR using R16F2n/R16R2 primers was chosen to be cloned and sequenced for further identification and classification of phytoplasma. BLASTn analysis showed that the phytoplasma from Opuntia sp. was closely related to 16SrII group. Phylogenetic analysis and in silico RFLP indicated that phytoplasma strain infecting Opuntia sp. was a member of subgroup 16SrII-C (cactus witches’ broom phytoplasma). This is a newly report of cactus witches’ broom phytoplasma on Opuntia sp. in Indonesia.

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