cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Ummy Mardiana Ramdan
Contact Email
joimedlabs.aiptlmi@gmail.com
Phone
+6281223433385
Journal Mail Official
joimedlabs.aiptlmi@gmail.com
Editorial Address
Jl. Arteri JORR Jatiwarna Pondok Melati, Bekasi, Jawa Barat, Indonesia Kode Pos: 17415
Location
Unknown,
Unknown
INDONESIA
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 8 Documents
 1 
Search results for , issue "Vol 2 No 2: Oktober 2021" : 8 Documents clear
UJI DAYA HAMBAT EKSTRAK BAWANG DAYAK (Eleutherine palmifolia (L.) Merr.) TERHADAP BAKTERI Salmonella parayphi Nor Aini Aida; Febri Nur Ngazizah; Miftachul Sobirin; Riky Riky
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.48

Abstract

Dayak onions (Eleutherine palmifolia (L.) Merr.) Is one of the natural ingredients which is often used for medicine in Kalimantan. E.  palmifolia used for treatment because it contains secondary metabolite compounds that have potential as antibacterial. Such as phenolic compounds, flavonoids, alkaloids, saponins, tannins and glycosides which are included in polar compounds. This study aims to determine the effect of ethanol extract 70% E. palmifolia against bacteria Salmonella paratyphi with the extraction method maceration. S. paratyphi is a bacterial pathogen against humans whose habitat is in the digestive tract. This research was conducted to test the extract's antibacterial activity with gradient concentration of 20 mg / mL, 30 mg / mL, 40 mg / mL, 50 mg / mL, and 60 mg / mL and chloramphenicol as a positive control and sterile aquadest as a negative control.  The antibacterial test method used is diffusion method with 5 repetitions. Our results showed that E. palmifolia extract could inhibit S. paratyphi growth as evidenced by the presence of an inhibition zone that is formed around paper disk on media with concentration 20 mg / mL, 30 mg / mL, 40 mg / mL, 50 mg / mL, and 60 mg / mL. Where the average diameter of the inhibition zone was 6.6 mm, 7.4 mm, 8.8 mm, 9.9 mm, respectively fall into the category of ineffective inhibition and 11 mm is in the weak category. Based on the research that has been done, it was found that there was an effect of giving E. palmifolia extract on the growth of S. paratyphi bacteria but there was no significant difference based on the one way ANOVA test.
ANALISA EKSTRAK ETANOL DAUN BAJAKAH KAIT-KAIT (Uncaria acida (Hunt.) Roxb.) TERHADAP BAKTERI Escherichia coli MENGGUNAKAN METODE KIRBY BAUER Mia Irawan; Febri Nur Ngazizah; Riky Riky; Iqlila Romaidha
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.49

Abstract

Kalimantan forest is the largest tropical rain forest which is one of the places with biodiversity that has the potential for development in the field of health or medicine, one of the plants used for treatment is hook root (Uncaria acida (Hunt.) Roxb.). This plant has indole alkaloid compounds, triterpenes, flavonoids and phenylpropanoids that act as antibacterial. In this study, an antibacterial test was conducted using the Kirby Bauer method to determine the antibacterial activity which was characterized by the formation of an inhibition zone around the paper disc. The bacteria tested were E. coli. The extract tested in this study was the ethanolic extract of U. acida leaves with 5 concentrations of treatment (5 mg/mL, 15 mg/mL, 30 mg/mL, 40 mg/mL and 50 mg/mL). The average result of the inhibition zone of the ethanolic extract of U. acida leaves in a concentration of 5 mg/mL is 8.1 mm in the weak category, the concentration of 15 mg/mL is 8.3 mm in the weak category, the concentration of 30 mg/mL is 9.1 mm in the weak category, 40 mg/mL ie 9.2 mm in the weak category and 50 mg/mL in the moderate category. Using significance (? > 0.05) which means that the inhibition zone data on the growth test of E. coli bacteria at the 5 concentrations affected the inhibition zone of E. coli bacteria.
PERBANDINGAN EFEKTIVITAS EKSTRAK BAWANG PUTIH (Allium sativum L.) DENGAN DAUN BAJAKAH (Uncaria acida Roxb.) SEBAGAI LARVASIDA VEKTOR DENGUE Aedes aegypti Fitra Akbar Mulyono; Iqlila Romaidha; Nur Aini Hidayah Khasanah; Febri Nur Ngazizah
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.50

Abstract

Dengue fever is a disease caused by the dengue virus carried by the A. aegypti mosquito. Making natural larvicides from plants such as Alium sativum L. and Uncaria acida Roxb. are indispensable. This study aims to make natural larvicides from A. sativum and U. acida. This study is an experimental study using a Completely Randomized Design (CRD) to see the effectiveness of the extract A. sativum with U. acida as larvicide of the mosquito A. aegypti.This study used 20 mosquito larvae for each concentration and repeated 4 times. The results showed that the larvicide of A. sativum at the highest concentration reached 100% mortality, while the larvicide of U. acida at the highest concentration reaching 80% mosquito larvae death A. aegypti. Analysis of research data was processed using SPSS version 20. One Way Anova analysis of A. sativum extract was sig 0.049 and U. acida extract was sig 0.245. In the extract of A. sativum there was a significant difference in the mortality of mosquito larvae at each concentration, while in the extract of U. acida there was no significant difference in the mortality of mosquito larvae at each concentration. T-test analysis of the extracts of A. sativum and U. acida was sig 0.80, so there was no difference in effectiveness between the two larvicidal extracts. It can be concluded that the larvicidal research results of A. sativum extract and U. acida extract were equally effective on A. aegypti larvae.
PERBEDAAN DARAH SEGERA DIPERIKSA, DILAKUKAN PENYIMPANAN PADA SUHU 20ºC-25ºC DAN 4ºC-8ºC SELAMA 6 JAM TERHADAP JUMLAH ERITROSIT Ferdi Afriansyah; Bastian Bastian; Indah Sari; Denny Juraijin
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.51

Abstract

Errors that often occur in laboratory examinations are in the pre-analytic stage with an error percentage of 50%-75%. Examination of blood samples to maintain conditions so that they are not damaged, the samples are usually stored at a temperature 4-8oC for several hours to 4-5 days and blood specimens stored temperature of 20-24oC for a maximum examination 24 hours. However, the examination of the number of erythrocytes should be carried out immediately after collection without storage because the delay in examination time can affect the number of erythrocytes, the longer the delay, the number of erythrocytes decreases because the cells undergo hemolysis, biochemical, biomechanical and immunological reactions. This researcher aims to determine the difference in blood immediately examined, blood stored temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes. The research design used a true experiment with a pre-test design and a post-test control group design with a hypothesis test using the Two-way ANOVA test. This research was conducted 30 respondents of DIV Medical Laboratory Technology students. The results of the study the number of erythrocytes in the blood immediately with an average value of 4.57 million/µI, at a temperature of 20oC-25oC has an average value of 4.65 million/µL, temperature of 4oC-8oC has an average value of 4.50 million/µL and Friedman test obtained p value = 0.172. Conclusion of the study showed that there was no difference in blood immediately examined, the blood was stored at a temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes.
MODIFIKASI METODE ISOLASI DNA CETYL TRIMETHYLAMMONIUM BROMIDE (CTAB) UNTUK SAMPEL EPITEL PIPI MANUSIA Maharani Pertiwi Koentjoro; Hidayah Sri Wilujeng; Astrina Dilla; Endry Nugroho Prasetyo
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.54

Abstract

Isolation of deoxyribonucleotide (DNA) is an important step in molecular analysis. In this process, DNA must be obtained in sufficient quantities and in good quality for any further analysis. The Cetyl Trimethylammonium Bromide (CTAB) method is commonly used in DNA isolation of plant or fungal. This method is an alternative in DNA isolation since it is easy and inexpensive. This study aims to modify the CTAB method for DNA isolation from human cheek epithelium for any molecular analysis. Epithelial cells were taken from the oral cavity of the researcher. The isolation protocol included cell lysis step with CTAB buffer and proteinase-K, purification step with the addition of chloroform:isoamylalcohol (24:1), precipitation step with isopropanol. The results of the ratio analysis of DNA spectrophotometer at wavelengths of 260 and 280 nm in the range of 1.73-1.85. The quality of DNA isolation was observed by agarose gel electrophoresis and a firm band was obtained after Ethidium Bromide staining. The DNA concentration in both methods ranged from 400-480 mg/mL. The time required for both methods ranges from 2.5-3 hours. The modified CTAB method DNA isolation protocol produces DNA that has good quality and quantity for molecular analysis processes, such as Polymerase Chain Reaction (PCR).
EFEKTIVITAS VARIASI KONSENTRASI TEPUNG TALAS SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Candida albicans Marchamtia Sarah Nur Awalia Fajari; Muhammad Taufiq Qurrohman
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.56

Abstract

Taro flour (Colocasia esculenta (L.) Schott) has 77%% carbohydrate. A high carbohydrate source has the potential to be used as an alternative media in supporting the examination of Candida albicans fungal culture. Candida albicans is one of the fungi causing candidiasis. Therefore, this study aims to see the effectiveness of the variations of taro flour concentrations as an alternative media to the growth of fungus Candida albicans. The type of the study was experimental with a post test only control group design. The subjects of the study were taro flour media with concentrations of 0,12%, 0,24%, and 0,36% tested on fungus Candida albicans, which was inoculated by spread plate with PDA (Potato Dextrose Agar) media used as control. The data collection was performed by calculating the colony of Candida albicans, which grew in each media. The mean colony of each media on PDA, taro flour 0,12%, 0,24%, 0,36% were 15.5 colonies, 18.8 colonies, 19.5 colonies, and 20.8 colonies, respectively. The effectiveness test showed that all taro flour concentrations have a percentage more than 100%. The variation of taro flour has the growth effect of fungus Candida albicans. Thus, the higher the concentration of taro flour media, the more colony the number of Candida albicans grew. The effectiveness test showed that all taro flour concentrations was classified as very effective.
APLIKASI METRIK SIGMA DALAM PEMANTAPAN MUTU INTERNAL PADA PEMERIKSAAN UREUM DISALAH SATU LABORATORIUM RUMAH SAKIT KABUPATEN PANGANDARAN Rizal Apriansyah Pratama; Dewi Kania Yulianti; Doni Setiawan
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.64

Abstract

Intralaboratory Quality Control in clinical laboratories is significant and helps detect errors early to improve quality and guarantee examination results. The guaranteed method can be done by applying the sigma metric, which can detect 690,000 errors per million opportunities or 30.9 probability without defects with a minimum sigma value category of 3 and a maximum sigma value of 6. Urea is one of the clinical chemistry examination parameters that often has a sigma value <3. This study aims to determine the application of the sigma metric in internal quality assurance on urea examination in one of the Pangandaran district hospital laboratories. This research method is a descriptive-analytic based on the sigma value in internal quality assurance on urea examination in Pangandaran district hospital laboratories. Based on the study results, the value of sigma 3 - < 4 or marginal in March and May 2021 at the average control level while at the pathological control level increased from 2 sigma in March to 3 sigma in May 2021 said to be marginal.
PENGGUNAAN SIX SIGMA PADA PEMERIKSAAN JUMLAH LEUKOSIT DI RSUD PANEMBAHAN SENOPATI BANTUL Hieronymus Rayi Prasetya; Nurlaili Farida Muhajir; Magdalena Putri Iriyanti Dumatubun
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.72

Abstract

Internal quality assurance is a prevention and control activity that must be carried out by the laboratory continuously and covers all aspects of laboratory examination parameters. Hematology examination in the laboratory is carried out using a Hematology analyzer, but this tool has limitations, one of which is that it can make leukocyte count reading errors. In order for the results of the tool to be reliable, it is necessary to carry out quality control on the hematology analyzer. The use of Westgard multirule is commonly used in laboratories, but the application of six sigma is still very rarely used, especially in the field of hematology. This research aims to know the internal quality control of the analytical stage of the Hematology analyzer for the leukocyte count based on the analysis of Westgard and Six sigma. This type of research is descriptive research. The sample in this study is the control value data for the examination of the leukocyte count for 1 month at Panembahan Senopati Hospital. The data were analyzed using the Westgard rules and Six sigma. At low level, 13s deviation (random error) is obtained. At the normal level, there is a deviation of 12s (warning). At high level 12s deviation is obtained (warning). The sigma scale at all control levels shows a scale above 6. Analysis based on six sigma for leukocyte count showed an average of 7.16 sigma which indicates that leukocyte examination using a hematology analyzer has an accuracy of 99.9%.

Page 1 of 1 | Total Record : 8

 1 

Filter by Year

2021 2021


Reset
Filter By Issues
All Issue Vol 6 No 2: Oktober 2025 Vol 6 No 1: April 2025 Vol 5 No 2 (2024): In Progress Vol 5 No 1: April 2024 Vol 4 No 2: Oktober 2023 Vol 4 No 1: April 2023 Vol 3 No 2: Oktober 2022 Vol 3 No 1: April 2022 Vol 2 No 2: Oktober 2021 Vol 2 No 1: April 2021 Vol 1 No 1: Oktober 2020 More Issue