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<![CDATA[IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS ]]>
<![CDATA[Darwin, Bastian]]>;
<![CDATA[Kurniati, Nova]]>;
<![CDATA[Rahadiyanto, Kemas Ya'kub]]>;
<![CDATA[Saleh, Mgs Irsan]]>;
<![CDATA[Tanoehardjo, Francisca Srioetami]]>;
<![CDATA[Nugraha, Jusak]]>;
<![CDATA[Salim, Eddy Mart]]>
<![CDATA[ Biomedical Journal of Indonesia Vol 6, No 2 (2020) ]]>
Publisher : <![CDATA[Fakultas Kedokteran Universitas Sriwijaya ]]>
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DOI: 10.32539/bji.v6i2.10741
<![CDATA[The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.]]>
<![CDATA[IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS ]]>
<![CDATA[Darwin, Bastian]]>;
<![CDATA[Kurniati, Nova]]>;
<![CDATA[Rahadiyanto, Kemas Ya'kub]]>;
<![CDATA[Saleh, Mgs Irsan]]>;
<![CDATA[Tanoehardjo, Francisca Srioetami]]>;
<![CDATA[Nugraha, Jusak]]>;
<![CDATA[Salim, Eddy Mart]]>
<![CDATA[ Biomedical Journal of Indonesia Vol 6, No 2 (2020) ]]>
Publisher : <![CDATA[Fakultas Kedokteran Universitas Sriwijaya ]]>
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DOI: 10.32539/bji.v6i2.10741
<![CDATA[The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.]]>
<![CDATA[Correlation The Number Of Erythrocytes And Glucose Level From Serum Which 2 Hours Delayed From Delayed ]]>
<![CDATA[Bastian Darwin]]>;
<![CDATA[Eka Yuniar]]>;
<![CDATA[Endang Endang]]>;
<![CDATA[Ian Kurniawan]]>
<![CDATA[ Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 1 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS) ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Mataram ]]>
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DOI: 10.32807/jambs.v7i1.164
<![CDATA[In the processing of specimens, serum specifically should be separated from blood cell the process of glucose glycolysis which caused decreasing levels of glucose. The very high number of erythrocyte can cause a glycolysis, so the decreasing of the levels of glucose will be happen. This study was pre-experiment using One Group Pretest Posttest. The sample was taken using purposive sampling technique and there were 30 subjects. The sample was EDTA bloods and serum which had been treated such as Separation of Glucose Serum which was 2 hours delayed and Separation of Glucose Serum which was checked directly. From this study we can found that the average of the glucose levels on serum separation which is 2 hours delayed was 91 mg/dL and the Separation of Glucose Serum which is checked directly was 97mg/dL. Based on paired T test the result, the data showed that there were differences from average levels of glucose between serum which was checked directly and separation of Serum which was 2 hours delayed. The average from the number of erythrocyte was 5,0 million/µL. Based on pearson correlation, there was no correlation between the number of erythrocyte and glucose level on serum separation which is 2 hours delayed.]]>
<![CDATA[Efektivitas Madu Dalam Kemasan Terhadap Rasa Tanpa Testis Bakteri Salmonella typhi ]]>
<![CDATA[Bastian Darwin]]>;
<![CDATA[Melli Marlina]]>
<![CDATA[ Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS) ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Mataram ]]>
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DOI: 10.32807/jambs.v8i2.241
<![CDATA[Demam tifoid atau typhus abdominalis adalah suatu infeksi akut yang terjadi pada usus halus disebabkan oleh bakteri gram negatif Salmonella typhi yang ditransmisikan melalui makanan maupun minuman yang terkontaminasi oleh feses atau urin dari orang terinfeksi. Madu kemasan adalah madu yang dibuat dari madu murni pilihan yang sebelumnya telah diproses sedemikian rupa secara steril dan higienis kemudian dikemas dengan berbagai ukuran dan harga yang lebih terjangkau dari pada madu alami. Penelitian ini bertujuan untuk mengetahui efektivitas madu kemasan terhadap daya hambat bakteri Salmonella typhi. Jenis penelitian ini dilakukan dalam bentuk eksperimen murni yang dianalisa secara diskriptif kuantitatif dan disajikan dalam bentuk diagram sederhana. Penelitian dilakukan di Laboratorium Mikrobiologi Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang dengan menggunakan metode disk difusi cakram kertas. Hasil penelitian ini didapatkan rata – rata efektivitas madu kemasan terhadap daya hambat bakteri Salmonella typhi pada konsentrasi 25% tidak terbentuk, 50% sebesar 2,4 mm dan 75% sebesar 5,4 mm dengan uji wilcoxon didapatkan nilai p = 0,006. Kesimpulan dari penelitian ini yaitu madu kemasan dengan konsentrasi 25%, 50%, dan 75% tidak memiliki efektivitas terhadap daya hambat bakteri Salmonella typhi.]]>
<![CDATA[Pemanfaatan Serum Hemolisis Dengan Penambahan Reagen Anti-Rh Terhadap Kadar Enzim Serum Glutamic Pyruvic Transminase (SGPT) ]]>
<![CDATA[Bastian Bastian]]>;
<![CDATA[Indah Sari]]>;
<![CDATA[Helnia Sari]]>;
<![CDATA[Juwy Trianes]]>
<![CDATA[ THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surabaya ]]>
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DOI: 10.30651/jmlt.v4i2.9745
<![CDATA[Serum glutamate pyruvate transferase (SGPT) also known as alanine aminotransferase (ALT) is an enzyme found in liver cells and is effective in diagnosing hepatocellular destruction. Examination of SGPT activity using blood serum is often difficult due to insufficient blood volume or lysed serum conditions due to improper collection. This study aims to determine whether there is use of serum hemolysis using anti-rh reagents in the examination of SGPT enzyme levels. This type of research uses an experimental design - static group comparison. The sample will be taken by students of DIV Medical Laboratory Technology IKest Muhammadiyah Palembang. The research was carried out starting from patient preparation, taking examination materials, processing examination materials, analysis and examination results. The results of the ANOVA test showed that the significant value was p = 0.000. The p value obtained was p> 0.005. The results of the study found that there was a significant difference in the amount of SGPT in normal serum and serum with the addition of anti-Rh to hemolysis serum]]>
<![CDATA[Perbedaan Kadar Trigliserida Pada Sampel Darah Segera Disentrifugasi Dan Sampel Darah Dibekukan Selama 20 Menit Sebelum Disentrifugasi ]]>
<![CDATA[Reni Junika Familianti]]>;
<![CDATA[Indah Sari]]>;
<![CDATA[Bastian Bastian]]>
<![CDATA[ THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surabaya ]]>
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DOI: 10.30651/jmlt.v4i2.9580
<![CDATA[Trigliserida atau yang sering disebut triasilgliserol adalah salah satu jenis lemak yang terdapat dalam darah dan berbagai organ tubuh yang dibentuk dari gliserol dan lemak yang ada dalam makanan yang dikonsumsi secara berlebihan. Nilai trigliserida yang tinggi memiliki kaitan dengan timbulnya penyakit serangan jantung, stroke dan diabetes. Penelitian ini bertujuan untuk mengetahui perbedaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan selama 20 menit yang dilakukan di Balai Besar Laboratorium Kesehatan Palembang. Jenis penelitian yang digunakan adalah Cross sectional komparatif, dengan rancangan penelitian intact group comparison. Sampel berupa 30 serum segera disentrifugasi dan 30 serum dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Penelitian ini dilakukan mulai dari persiapan sampel, pengambilan bahan pemeriksaan, pengolahan bahan pemeriksaan, analisis dan hasil penelitian. Kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel serum segera disentrifugasi yaitu 0.826 mmol/L sedangkan kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel dibekukan 20 menit sebelum disentrifugasi yaitu 0.918 mmol/L. hasil uji dependent t test diketahui bahwa nilai signifikan adalah p = 0,342. Nilai p yang didapatkan adalah p > 0,05. Hasil penelitian dapat disimpulkan bahwa tidak ada perbedaan hasil pemeriksaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Kata kunci : Kadar Trigliserida, Segera Sentrifugasi, Dibekukan 20 MenitDaftar Pustaka: 31 (2013-2020)]]>
<![CDATA[Education on The Identification of Evidence of Blood Spots Against the Determination of Blood Type ]]>
<![CDATA[Indah Sari]]>;
<![CDATA[Bastian Bastian]]>;
<![CDATA[Veny Tresia Utari]]>
<![CDATA[ ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2022): ABDIMAS UMTAS: Jurnal Pengabdian Kepada Masyarakat ]]>
Publisher : <![CDATA[LPPM Universitas Muhammadiyah Tasikmalaya ]]>
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DOI: 10.35568/abdimas.v5i1.2078
<![CDATA[Knowing a person's blood type is very important to know for medical purposes. Blood type is important to know, for the sake of transfusion, the right donor and identification in forensic medical cases such as identification in some criminal cases. Blood stains have an important role in revealing various legal and criminal cases. In the process of identifying bloodstains as evidence, the first thing that must be done is to determine whether the bloodstains actually contain blood molecules so that the blood group can then be identified. Blood group identification is generally done based on the ABO blood grouping system. There have been many criminal cases that have been revealed through the ABO blood group identification system. Blood type examination is one of the methods used when a crime case occurs in the community. There are 4 human blood groups (A, B, AB, and O) which are grouped based on the presence or absence of antigens on the surface of erythrocytes. Identification of blood group ABO system on fresh blood samples is easier to do than dried blood samples. This is because the cells in the dried blood are damaged. However, it is still possible to identify the blood group of dry blood samples. This is because the antigens on the surface of red blood cells remain intact even though the cells have been destroyed. The method commonly used to identify the blood group of dry blood samples is the elution absorption method. This method is a very sensitive method and can detect the presence of antigens indirectly.]]>
<![CDATA[Analisis Aktivitas Fisik Ringan dan Berat Terhadap Kadar Hemoglobin ]]>
<![CDATA[Heriyanto,]]>;
<![CDATA[Indah Sari,]]>;
<![CDATA[Aristoteles]]>;
<![CDATA[Bastian]]>
<![CDATA[ Jurnal Kesehatan Saelmakers PERDANA (JKSP) Vol. 5 No. 1 (2022): Jurnal Kesehatan Saelmakers PERDANA ]]>
Publisher : <![CDATA[Fakultas Ilmu Kesehatan Universitas Katolik Musi Charitas ]]>
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DOI: 10.32524/jksp.v5i1.406
<![CDATA[Physical activity is all body movements caused by skeletal muscles with the need for energy expenditure. Physical activity is divided into 3 categories. Light, moderate and heavy physical activity. Heavy physical activity will increase metabolic activity, the acid produced in the form of hydrogen ions and lactic acid will increase a lot, this will cause a decrease in pH and intravascular hemolysis. The affinity between oxygen and hemoglobin will decrease when the blood pH is low. Hemoglobin is a tetrameric protein of erythrocytes that binds to non-protein molecules, namely iron porphyrin compounds called heme. This study aims to determine the effect of light and heavy physical activity on hemoglobin levels in students of the Faculty of Science and Technology IKest Muhammadiyah Palembang. This type of research is carried out in the form of experiments which are analyzed descriptively quantitatively and presented in the form of simple tables and diagrams. The sample was examined using a Hematology Analyzer with the Alfa Swelab Plus brand, the Pothomertic method. The results showed that the average hemoglobin level for light physical activity was 15.01g/dL and heavy physical activity was 15.2 g/dL with the One Way Anova test, the value = 0.689. The conclusion of the study showed that there was no effect between light and heavy physical activity on hemoglobin levels in students of the Faculty of Science and Technology IKest Muhammadiyah Palembang.]]>
<![CDATA[ANALISA METODE FIKSASI KERING MENGGUNAKAN GIEMSA DAN FIKSASI BASAH MENGGUNAKAN PAPANICOLAOU PADA PEMERIKSAAN PAP SMEAR ]]>
<![CDATA[Indah Sari]]>;
<![CDATA[Bastian Darwin]]>;
<![CDATA[Try Era Realita]]>
<![CDATA[ Masker Medika Vol 9 No 1 (2021): Masker Medika ]]>
Publisher : <![CDATA[IKesT Muhammadiyah Palembang ]]>
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DOI: 10.52523/maskermedika.v9i1.456
<![CDATA[Kanker serviks adalah penyakit yang ditandai dengan pertumbuhan sel yang tidak terkontrol dan penyebaran sel abnormal yang terjadi di serviks. World Health Organization (WHO) menjelaskan bahwa kanker serviks merupakan penyebab kematian nomor dua pada perempuan dari seluruh penyakit kanker yang ada. Pap smear merupakan pemeriksaan yang dilakukan untuk mendeteksi kanker serviks. Pemeriksaan pap smear menggunakan bahan pemeriksaan swab vagina yang harus dilakukan fiksasi basah sebelum pewarnaan papanicolaou dan fiksasi kering sebelum pewarnaan giemsa. Penelitian bertujuan untuk menganalisa metode fiksasi basah menggunakan pewarnaan papanicolaou dan fiksasi kering menggunakan pewarnaan giemsa pada pemeriksaan pap smear yang dilakukan di Laboratorium Patologi Anatomi Dyatnitalis Palembang. Jenis penelitian menggunakan cross sectional dengan rancangan penelitian menggunakan intact group comparison. Sampel terdiri atas 8 responden masing-masing dilakukan fiksasi basah diwarnai dengan papanicoloau dan fiksasi kering diwarnai dengan giemsa. Penelitian dilakukan mulai dari persiapan pasien, pengambilan bahan pemeriksaan, pengolahan bahan pemeriksaan, analisis dan hasil pemeriksaan. Hasil uji Chi-Square diketahui bahwa nilai signifikan adalah p = 0,064. Nilai p yang didapatkan adalah p˃0,05. Hasil penelitian dapat disimpulkan bahwa tidak terdapat perbedaan antara metode fiksasi kering menggunakan giemsa dan fiksasi basah menggunakan papanicolaou pada pemeriksaan pap smear.]]>
<![CDATA[ANALISA JUMLAH KOLONI BAKTERI Escherichia coli PADA MEDIA NUTRIENT AGAR DENGAN PELARUT AKUADES DAN AIR MINUM DALAM KEMASAN (AMDK) ]]>
<![CDATA[Bastian Darwin]]>;
<![CDATA[Indah Sari]]>;
<![CDATA[Annisa Fitri]]>
<![CDATA[ Masker Medika Vol 9 No 1 (2021): Masker Medika ]]>
Publisher : <![CDATA[IKesT Muhammadiyah Palembang ]]>
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DOI: 10.52523/maskermedika.v9i1.457
<![CDATA[Pendahuluan: Penyakit diaere yang di sebabkan Escherichia coli patogen bahwa ada sekitar dua miliar kasus diare diseluruh dunia setiap tahun, dan 1,9 juta anak balita meninggal akibat diare setiap tahunnya. Standar pelarut dalam pembuatan media nutrient agar adalah akuades, Tetapi di daerah-daerah tertentu masih belum terjangkau untuk mendapatkan akuades, selain harganya yang mahal jangkauan untuk mendapatkan akuades tidaklah mudah karena alat untuk pembuatan akuades masih cukup mahal. upaya yang dapat dilakukan adalah menggunakan pelarut alternatif, dimana air minum dalam kemasan adalah air baku yang telah diproses, dikemas, dan aman diminum. Tujuan untuk mengetahui perbedaan jumlah koloni bakteri E.coli pada media nutrien agar dengan pelarut akuades dan air minum dalam kemasan. Metode Penelitian: Eksperimen laboratorium, yang dilakukan di Laboratorium Mikrobiologi Fakultas Sains dan Teknologi Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang. Sampel berupa Strain Murni Bakteri Escherichia coli ATCC 25922. Hasil: penelitian didapatkan jumlah koloni pada media nutrient agar dengan pelarut akuades mendapatkan nilai rata-rata 54 koloni, pada pelarut air minum dalam kemasan mendapatkan rata-rata 59 koloni. Dan uji-T berpasangan (paired sampel T test) di dapatkan nilai p =>0.112. Kesimpulan: Penelitian ini bahwa tidak terdapat perbedaan signifikan jumlah koloni bakteri E.coli pada media nutrient agar dengan pelarut akuades dan air minum dalam kemasan]]>
