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Ummy Mardiana Ramdan
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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 8 Documents
 1 
Search results for , issue "Vol 6 No 1: April 2025" : 8 Documents clear
Analisis Unit Cost Quality Control Kimia Klinik dengan Metode Activity Based Costing di Laboratorium Rumah Sakit Paru dr. H. A. Rotinsulu Suryadi, Arfiah Azzahra Nissa; Kurniawan, Entuy; Rahayu, Ira Gustira; Rinaldi, Sonny Feisal; Hidayatullah, Taufik
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.192

Abstract

Hospital laboratories must comply with quality and safety standards as they are responsible for patients' health test results. Laboratory test results must be reliable, so the quality of laboratory services needs to be improved, including implementing quality control measures. Conducting a unit cost analysis for quality control activities is necessary to determine the expenses incurred for each quality control activity. The objective of this writing is to determine the unit cost for Clinical Chemistry Quality Control in the laboratory using the Activity-Based Costing method. Data collection techniques include interviews, observations, and document studies. Various costs are involved in determining the ABC method, including labor costs, depreciation costs for equipment or buildings, and other costs. In addition to cost data, cost drivers are also important in determining them. The equipment used in this research is the Sysmex BX with a total of 14 examination parameters, including glucose, total bilirubin, direct bilirubin, SGOT / AST, SGPT / ALT, total protein, albumin, urea, creatinine, uric acid, cholesterol, triglycerides, HDL, LDL. Based on the research results, the cost of QC for clinical chemistry examinations is Rp. 102,477
Optimalization of NaOH concentration in alkaline lysis method on quality and quantity of Candida albicans DNA Putri, Nasya Desinta; Prayekti , Endah
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.223

Abstract

Background: Candida albicans was a major caused of invasive candidiasis whose discovery by culture took a longed timed. Polymerase Chain Reaction (PCR) was a deoxyribonucleicacid (DNA)-based rapid diagnostic method. The simple DNA isolation method of alkaline lysis needed to optimized for Candida albicans, which had a complex cell wall that is difficult to broke. The concentration of NaOH in the alkaline lysis method is one of the things that affects the results of DNA isolation. Objective: The purpose of this studied was to determine the effect of optimizing the concentration of NaOH in the alkaline lysis method on the quality and quantity of Candida albicans DNA. Materials and Methods: This researched is experimental by performed DNA isolation used alkaline lysis method used NaOH concentrations of 1.5 N, 1.75 N, and 2.0 N and controlled (NaOH 0.2 N). Calculation of DNA quality and quantity using a nanodrop spectrophotometer. Results: The results showed the ordered of obtained the high to low DNA quality (A260/280) was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (1.86±0.44-0.95±0.18) while for DNA quantity was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (187.7±58.3-9.6±3.5 ng/ml). Conclusion: There is an effect of optimizing NaOH concentration on the lysis of fungal cell walls in the alkaline lysis method, namely increased the purity valued and concentration of sample DNA isolation results gradually. The use of 2.0 N NaOH concentration produced the best quality and quantity of DNA, namely 1.86±0.44 for DNA quality and 187.7±58.3 for DNA quantity.
The Relationship between C-Reactive Protein (CRP) Levels and HbA1c Levels in Type II Diabetes Mellitus Patients Utami, Rinda; Susanto, Zaenal Adi; Marsudi, La Ode; Maulida , Shofia; Mustofa , Khalid
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.234

Abstract

Background: Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia due to abnormalities in insulin secretion, insulin action, or both. Type II diabetes mellitus is caused by the pancreas gland being unable to meet the body's insulin needs. The HbA1c test reflects the average blood glucose level for 2-3 months or about 120 days before the test. C-reactive protein (CRP) is an inflammatory biomarker that is often used to assess the level of systemic inflammation. Elevated CRP levels have been associated with insulin resistance, endothelial dysfunction, and the development of atherosclerosis, all of which contribute to complications in diabetic patients. Therefore, further research is needed to evaluate the correlation between HbA1c and CRP levels, to better understand the relationship between glycemic control and systemic inflammation. Objective: This study aims to determine the relationship between C-reactive protein (CRP) levels and HbA1c levels in patients with Type II Diabetes Mellitus. Materials and Methods: This study used purposive sampling technique and analytic survey with cross-sectional approach. Results:  Data analysis using the Chi-Square test obtained a p-value of 0.536, where if Sig> 0.05 then it shows there is no significant relationship. So, there is no relationship between C-reactive protein (CRP) levels and HbA1c levels in Type II Diabetes Mellitus patients.
Effectiveness of Miana Leaves (Plectranthus scutellaroides) as a Natural Dye in the Identification of Soil Transmitted Helminth (STH) Eggs Samosir, Erica; Rahmah, Lestari
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.236

Abstract

Background: The use of natural dyes in microscopic examinations, such as the identification of Soil-Transmitted Helminth (STH) eggs, provides a safer and more environmentally friendly alternative. Miana Leaves (Plectranthus scutellaroides), which contain high levels of anthocyanins, have the potential to serve as an effective natural dye for staining STH eggs in fecal samples. Objectives: This study aims to evaluate the staining efficacy of miana leaf extract (Plectranthus scutellaroides) in identifying STH eggs by comparing different extract concentrations. Materials and Method: An experimental approach with descriptive data analysis was employed. Various concentrations of miana leaf extract (1:1, 1:2, and 1:3 ratios with distilled water) were tested on fecal samples positive for STH eggs. Results: The 1:2 concentration of miana leaf extract yielded optimal contrast and clear visualization of STH egg morphology, while the 1:1 concentration resulted in excessive staining and the 1:3 concentration produced pale staining. Conclusions: Miana leaf extract, particularly at a 1:2 concentration, is effective as a natural stain for STH egg identification. Further research should explore its application in broader parasitological diagnostic contexts.
Identification of Salmonella Sp. in Duck Eggs Sold in Larangan Village Putri, Nadia Auditia; Khusna , Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.245

Abstract

Background: Duck eggs are one of the foodstuffs that are very susceptible to damage caused by microbes. The types of microbes that contaminate eggs are mainly from the Gram-negative bacteria type, including Salmonella. Objectives: The purpose of this study was to determine the salmonella sp bacteria in duck eggs sold in Larangan village Materials and Methods: The examination method used was observation of laboratory tests, isolation methods, and identification of bacteria using biochemical tests. The samples used were duck egg yolks, using Brain Heart Infusion Broth (BHIB) media, Salmonella Shigella Agar (SSA), gram staining, Triple Sugar Iron Agar (TSIA), Simon citrate, and Sulfide Indol Motility (SIM). Results: The results of the research that has been done on BHIB media obtained 6 samples showed bacterial growth, on SSA media 6 duck egg samples showed bacterial colony growth, on gram staining obtained gram-negative and rod-shaped bacteria in 6 samples, on TSIA media obtained positive results on 6 media, simon citrate test showed positive results on 6 media, SIM test on motile test 6 media obtained positive results, on sulfide test 5 media obtained positive results, while indole test 6 media obtained negative results. Conclusions: Based on the results of research on the identification of salmonella sp bacteria in duck eggs sold in Larangan village, Larangan sub-district, Brebes regency, Central Java, it was found that 6 duck egg samples contained salmonella sp bacteria.
Identifikasi bakteri Escherichia coli dan Salmonella sp. pada Spons Pencuci Piring Berdasarkan Lama Penggunaan Turrohmah, Alhidayah; Khusna, Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.246

Abstract

Background: Sponges are tools used to clean cutlery. Dishwashing sponges are the main source of bacterial cross-contamination because they can transmit foodborne pathogens. Sponges that are used for a long time will have more potential to grow various microorganisms, such as bacteria from the Enterobacteriaceae family, including Escherichia coli and Salmonella sp. Objectives: The purpose of this study was to identify Escherichia coli, Salmonella sp..and the proper duration of use on sponges to avoid bacterial contamination. Materials and Methods: The type of research is descriptive, with a Quota sampling technique with a sample size of 6 dishwashing sponges at 6 points in the house (2 samples at one time), which have been determined on three indicators, namely 3,5,7 weeks of use. Observation and testing data collection methods. Results: The results showed negative Esherichia coli bacteria in all sponge samples with a duration of use of 3, 5, and 7 weeks, while positive Salmonella sp. bacteria in 1 sponge sample with a duration of use of 7 weeks. Conclusions: There were Salmonella sp. bacteria in 1 sample, namely a sponge used for 7 weeks and no Escherichia coli bacteria were found in all samples, all samples of dishwashing sponges with a usage period of 3, 5, and 7 weeks tested positive for bacterial contamination, so the minimum usage period for the sponge is 2 weeks, to avoid bacterial contamination from the dishwashing sponge.
Hubungan Personal Hygiene Dengan Kejadian Kecacingan Pada Anak Usia 6-9 Tahun Di Desa Pesarean Pagerbarang Tegal Jawa Tengah Khasanah, Isma Laelatul; Khusna , Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.248

Abstract

Background: Ringworm disease is one of the diseases caused by infections with Soil Transmitted Helminths (STH) which usually attacks children because they are less able to maintain Personal Hygiene such as still liking to play in the soil,and not washing hands after playing.Objectives: : The aim of this research is to determine the relationship between personal hygiene and the incidence of worms in children aged 6-9 years in Pesarean Pagerbarang Village, Tegal, Central Java.Materials and Methods: This study uses an Observational Analytical research type using a cross-sectional approach , with a Incidental Sampling technique.Results: Based on the results of the questionnaire interview, it was found that 17 children (48.57%) had good personal hygiene, and 18 children (51.43%) had poor personal hygiene. Laboratory examination showed that 21 children (60.00%) were infected with STH, including Ascaris Lumbricoides, 18 children (85.71%). Tricuris Trichiura 1 child (4.76%), Necator Americaanus 2 children (9.52%). Conclusions: The results of analysis using chi-square obtained p-value = 0.000 for the relationship between personal hygiene and the incidence of worms and p-value = 0.013 for the relationship between nail cleanliness and the incidence of worms. This shows that there is a significant relationship between the two because ?<0.05. Keywords Incidence of worms; Personal hygiene..
MELANVEER : TEKNOLOGI PENDETEKSI PEMBULUH DARAH VENA UNTUK MENINGKATKAN PELAYANAN KESEHATAN) Adeline, Ivone; Maulana, Andri; Hartriningsih, Salzabila; Adi Prasetyanto, Wisnu
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.259

Abstract

Background: Intravenous (IV) procedures face challenges due to low nurse proficiency (50.8%) in Indonesian and difficulties in vein visualization, especially in patients with darker skin tones. Objectives: To develop an affordable, portable vein detection device, "Melanveer," capable of accurate visualization for all skin types. Materials and Methods: Melanveer uses near-infrared (NIR) light and Contrast Limited Adaptive Histogram Equalization (CLAHE) for real-time vein visualization. The prototype, made with 3D-printed components, was tested on 20 patients with varying skin tones (Very white to light white skin, Fair to light brown skin, and Dark to very dark brown skin). Results: Melanveer demonstrated 94% accuracy in vein detection, improved efficiency for medical staff, and enhanced patient comfort during IV procedures. Conclusions: Melanveer offers an innovative, cost-effective, and portable solution for accurate vein detection, addressing challenges in IV procedures and improving healthcare outcomes

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