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Elvi Rusmiyanto PW
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Protobiont: Jurnal Elektronik Biologi
Published by Universitas Tanjungpura
ISSN : -     EISSN : 23387874     DOI : http://dx.doi.org/10.26418/protobiont.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Protobiont merupakan jurnal peer-reviewed dan open access journal yang mempublikasikan hasil-hasil penelitian biologi tropis yang berkaitan dengan bidang: 1. botani, 2. zoologi, 3. ekologi, 4. mikrobiologi, 5. genetika, 6. ilmu lingkungan, dan 7. biologi konservasi. Jurnal Protobiont terbit setahun 3 kali, yaotu bulan Maret, Juli, dan November. Jurnal Protobiont diterbitkan dan dikelola oleh Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 23 Documents
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Search results for , issue "Vol 6, No 2 (2017)" : 23 Documents clear
Pertumbuhan dan Karakter Anatomi Mimosa Air (Neptunia oleracea Lour.) pada Air yang Terpapar Logam Aluminium (Al) Elvi Rusmiyanto Pancaning Wardoyo, Mutiara Nugie Septiani, Mukarlina,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20816

Abstract

Water mimosa (Neptunia oleracea Lour.) is one kind of aquatic plant which is potential to have adaptive ability to heavy metal exposure in water. Among heavy metals that can contaminate water is aluminum originated from the use of aluminum sulfate [Al2(SO4)3], commonly known as alum for water clarifying. The research ran for 2 months, from January to March 2017. The research used Completely Randomized Design which consisted of six aluminium sulfate concentration treatments: control, 50, 75, 100, 125, and 150 ppm. The research findings showed that aluminum had influence on N. oleracea morphologically, which was marked by the leaves becoming yellowish and the roots becoming softer. Anatomically, seen from the slide of the root, the treatment using the highest concentration resulted in a shrinkage of stele area up to 69,43%. In the slide of the plant stem, the aerenchyma was more distantly spread, and from the slide of the leaf there was a color change of the tissue to be brown. Nevertheless, N. oleracea still could survive in water with aluminum concentration of 150 ppm.
Pertumbuhan Kalus Tanaman Markisa (Passiflora sp.) dengan Penambahan Naphtalene Acetic Acid (NAA) dan 6-Benzyl Amino Purine (BAP) Riza Linda, Mariamah, Mukarlina,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20801

Abstract

Passiflora sp. is one of the herbaceous plants whose leaves contain secondary metabolites. This plant is widely exploited by the community for use as pharmaceuticals. Accordingly another alternative is required to isolate its metabolites and one of the alternatives that can be applied is technique of tissue culture. The purpose of this study is to investigate the effect of NAA, BAP and combination of NAA and BAP on callus growth of Passiflora sp.. This study is conducted in Aloe Vera Center Laboratory of Pontianak. Completely Randomized Design with 4 concentration levels of NAA and BAP specifically 0 M, 10-7 M, 5x10-7 M and 10-6 M was employed 3 times and 48 units of experiments were obtained. The findings indicate that all treatments yield callus with dense texture and generate a variety of color specifically tawny, green and greenish white.NAA, BAP and combination of NAA and BAP has no significant effect on the average time of callus growth. The combination of NAA and BAP has substantial effect on the average of callus wet basis. Concentration of NAA 5x10-7+ BAP 10-6 M and NAA 5x10-7+ BAP 10-7 M produce the highest wet basis in particular 2,4380 g and 2,2420 g. the combination of NAA and BAP has remarkable effect on callus dry basis and concentration of NAA 5x10-7+ BAP 10-6 M produce the highest average of dry basis particularly 0,0160 g.
Aktivitas Antibakteri Ekstrak Metanol Daun Sembung Rambat (Mikania micrantha H.B.K) Terhadap Pertumbuhan Bakteri Bacillus cereus IHB B 379 dan Shigella flexneri Mukarlina, Rosalina Yuliana Ayen, Rahmawati,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20833

Abstract

Bacillus cereus and Shigella flexneri is a pathogenic bacterium that often contaminate food, can cause infection and digestive disorders. One of herbal plant which can be use as an antibacterial is sembung rambat (Mikania micrantha). This research aimed to determine the antibacterial activity of M. micrantha leaf methanol extract on the growth B. cereus IHB B 379 and S. flexneri. This research was conducted from Februari to April 2017. The testing of antibacterial activity has been performed using paper disk diffusion method with some extract concentrations of 0.45;0.50;0.55;0.60 and 0.65 g/ml and positive control of antibiotic chloramphenicol 0.003 μg. The lowest concentrations of the extracts that have been able to inhibit the growth of B. cereus IHB B 379 are 0.65 g/ml and are 0.50 g/ml of S. flexneri with a very strong resitance response. The test result show that M. micrantha leaf methanol extract is bacteriostatic.
Potensi Ekstrak Daun Paku Resam (Gleichenia linearis Burm.) Sebagai Bioherbisida Pengendali Gulma Rumput Grinting (Cynodon dactylon (L.) Pers) ., Enny Tri Septiani, Mukarlina,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20798

Abstract

Gleichenia linearis is a wide leaf weed which contains chemical compound such as flavonoid, allelochemical, triterpenoid, saponin, tannin, alkaloid and steroid that can inhibit the growth of other plants as a bioherbicide. This study aims to determine the concentration of G. linearis leaf extract on germination and growth of seedling weed Cynodon dactylon. This study was conducted in a laboratory and a screen house of Biology Department of Mathematics and Natural Science Faculty in Chemical and Soil Fertility Laboratory of Agriculture Faculty and in Wood Technology Laboratory of Forestry Faculty of University of Tanjungpura Pontianak from September to November 2016. The study employed completely randomized design with 5 replicates consisting control, 0,1; 0,2 and 0,3 gr/ml (germination) than control, 0,1 and 0,2 gr/ml (growt). The research findings showed that effective concentration that can inhibit germination of weed C. dactylon is 0,3 gr/ml concentration and inhibit the growth of other plants is 0,2 gr/ml concentration.
Struktur Anatomi Akar, Batang dan Daun Gaharu (Aquilaria malaccensis Lamk.) yang Mengalami Cekaman Kekeringan Dwi Gusmalawati, Suharti, Mukarlina,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.19712

Abstract

Agarwood (Aquilaria malaccensis) is a commodity of Non-Timber Forest Products (NTFPs), which has the result of the agar with the best quality and high value. Drought stress can impede the growth of the agarwood plant which can affect its morphology and anatomy. This research aims to determine the anatomical structure of the root, stem and leaf of the agarwood (A. malaccensis) that experiences drought stress. The research was conducted from July to October 2016. The drought stress in treatment (KL 25%) resulted in cross section of roots and stems of agarwood to have the length and thickness of cortical cells that becomes larger with a rectangular shape, in addition, the treatment (KL 25%) also resulted in the size of mesophyll tissue cells to become smaller.
Komposisi Mikroalga Epilitik di Sungai Mentuka Kabupaten Sekadau Ari Hepi Yanti, Diah Pratiwi, Tri Rima Setyawati,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20822

Abstract

Ephilitic microalgae is a microalgae that lives on a stone surface and it has an important role in aquatic ecosystem. The aim of this study are to know the composition of ephilitic microalgae and the condition of physical-chemical water in Mentuka River. This study was conducted 3 months from November 2016 to January 2017. This study used purposive random sampling method. The result shows that ephilitic microalgae which found in Mentuka River can be classify into two classes, namely Bacillariophyceae (15 genera) and Zygnematophyceae (4 genera). Synedra has the highest of average abundance in Mentuka River and the lowest is Fragilaria. Diversity and Evenness Index of ephilitic microalgae in every station categorized medium (H’= 1,53-1,65 dan E=0,52-0,56), meanwhile categorized of Dominance Index is low (C=0,29-0,34). The environmental condition in Mentuka River supports the life of ephilitic microalgae with temperature 26-28˚C, stream discharge is about 6,1-7 m/s, pH is about 6,8-7,3, dissolved oxygen is about 6,1-7 mg/L and CO2 is 3,9-5,0 mg/L.
Studi Analisis Perilaku Mencuci Tangan Terhadap Kepadatan Koloni Bakteri Sebelum dan Setelah Mencuci Tangan Pada Mahasiswa Diah Wulandari Rousdy, Dessy Kartika, Rahmawati,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.19494

Abstract

Handwashing with soap is a healthy behavior that has scientifically been proved to prevent the spread of infectious diseases. Students who are active on campus have risk factors of being contaminated by bacteria. This research aims to determine the correlation between the number of bacterial colonies on the palms and handwashing practices among students, and the difference between the number of bacterial colonies before and after washing hands with soap. This research used a pour plate method in accordance with the Standard Plate Count Method. The samples were 35 students. Based on the t-test, handwashing with soap showed a decrease in the number of bacterial colonies than before washing hands with soap, namely 1751.3 CFU / cm2 becoming 905.5 CFU / cm2 after washing hands. The logistic regression analysis and descriptive test showed that there is a correlation between the number of bacterial colonies on the palms and hand washing practices among students.
Ragam Jenis Semut (Hymenoptera: Formicidae) di Lahan Ga mbut Alami dan Perkebunan Sawit di Kecamatan Sungai Ambawang Kabupaten Kubu Raya Ari Hepi Yanti, Dita Meilina, Tri Rima Setyawati,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20814

Abstract

Ants are one of bioindicators in peatland ecosystems. Peatlands are changing from natural conditions due to the oil palm plantations. This study aims to determine the diversity of ants in natural peatland and oil palm plantation at Sungai Ambawang District Kubu Raya. The research was located in 3 different sites, such as natural peatland, cleared peatland and the peatland that had been converted to oil palm plantation. Ants were collected by pit-fall trap, soil and leaf litter sieving, and bait trap method. There were 7 genera from 3 subfamilies (Dolichoderinae, Formicinae, and Myrmicinae) of ants found in this research. Dolichoderinae consisted of the genera Loweriella and Tapinoma, Formicinae consisted of Camponotus and Echinopla, while Myrmicinae consisted of Acanthomyrmex, Crematogaster, and Rhoptromyrmex. The Shannon diversity index (H’) of natural peatland was higher (H’ 1.12) than cleared peatland (H’ 0.00) and converted peatland (H’ 0.69). This indicates that the conversion of peatlands decreased ant species diversity.
Uji Antagonis Isolat Jamur Rizosfer Lokal Terhadap Phytophthora sp. Im5 dari Pangkal Batang Tanaman Jeruk Siam (Citrus nobilis var. microcarpa) Mukarlina, Imu Rohayatun M, Rahmawati,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20834

Abstract

Fungi isolate from members of species of Phytophthora sp. Im5 is one of the pathogens that cause Brown Rot Gummosis on siam citrus plant (Citrus nobilis var. microcarpa). The control of plant pathogens is biologically done by utilizing biological agents in form of rhizosphere fungi that have antagonistic activity. This research aims to determine the antagonistic activity of rhizosphere fungi ( isolate : Mucor sp. Im1, Penicillium sp. Im2, Penicillium sp. Im3, and Trichoderma sp. Im4 ) in controlling fungi isolate of species of Phytophthora sp.Im5. Isolation, identification and antagonistic test were conducted at Microbiology Laboratory, Faculty of Mathematics and Science of Tanjungpura University Pontianak from February to June 2017. This research used Completely Randomized Design with five treatments and three replications which obtained a total of 15 experimental units. The antagonistic test used double test methods. The highest antagonistic activity in suppressing the growth of fungi isolate Phytophthora sp. Im5 is fungi isolate Mucor sp. Im1 with a percentage of 45.48%.
Struktur Komunitas Makrofita Akuatik di Sungai Embau Kecamatan Hulu Gurung Kabupaten Kapuas Hulu Tri Rima Setyawati, Irvan Fitra Jayadi, Riza Linda,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20803

Abstract

Aquatic macrophyte is one of macro plants that grows in or in near that water. The study aimed to know structure of aquatic macrophyte community plants in Embau river district of Hulu Gurung, Kapuas Hulu Regency. The research sample were taken in april 2016 using cover area transect. There are 15 species from 12 families of aquatic macrophytes found in the research site. Thirteen types of these plants are emergent such as Calla sp., Cyperus pilosus L., Chiloscypus polyanthos L., Dichodontium sp., Donax canniformes, Eleocharis pervula R.Br, Fissidens toxifolius L. Vhal, Fluminea festutacea, Eleusine indica (L) Gaertn, Homalomena sp., Monosolenium terenum, Polygonum sp. dan Rosmarinus sp. The two types of submerged plants found in the sampling site are Glossostigma sp., and Elodea sp. Species of F. toxifolius L. Vhal is one of aquatic macrophytes that had the highest cover area transect (CAT) with 18,1% while Eleusine indica (L) Gaertn had the lowest CAT with 1,39%. According diversity index aquatic macrophyte in Embau river was moderate (H’= 1,18-1,8), Index of dominace was low (C= 0,21-0,44) and high level of evenest index (E= 0,44-0,69). The Embau river is the highest polluted heavy metal of Plumbum (Pb2+) in sediment with 13,4 mg/l.

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