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PROFIL HEMATOLOGI DAN PERTUMBUHAN IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN Nastiti Wijayanti, Diah Wulandari Rousdy,
SEMIRATA 2015 Prosiding Bidang Biologi
Publisher : SEMIRATA 2015

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Abstract

Gambut merupakan jenis tanah organik yang memiliki banyak potensi kandungan senyawa humat untuk dikembangkan.Asam humat yang terkandung dalam gambut diketahui mempunyai berbagai potensi biologis salah satunya sebagai stimulasi pertumbuhan hewan.Penelitian ini dirancang untuk mengetahui pengaruh pemberian asam humat dari tanah gambut Kalimantan terhadap respon pertumbuhan ikan mas (Cyprinus carpio Linn.) ditinjau dari parameter hematologi.Penelitian menggunakan Rancangan Acak Lengkap dengan empat perlakuan dan enam ulangan.Perlakuan terdiri dari kontrol normal dan perlakuan asam humat (1%, 3% dan 5% dari berat pakan).Pemberian asam humat dilakukan secara oral melalui pakan selama 21 hari. Hasil penelitian menunjukkan pemberian asam humat memberikan kenaikan berat badan ikan mas terbaik pada perlakuan asam humat 1% yakni sebesar 9,01%. Hasil uji hematologi menunjukkan jumlah eritrosit tertinggi diberikan oleh perlakuan asam humat 1% yakni 1,37x106 sel/mm3 begitupula nilai hematokrit tertinggi diberikan pada perlakuan asam humat 1% yakni sebesar 44,55%. Jumlah leukosit ikan mas menunjukkan nilai tertinggi pada perlakuan asam humat 3% yakni sebesar 60,83x103 sel/mm3. Namun perlakuan asam humat 3% menyebabkan penurunan kadar hemoglobin. Hasil penelitian ini mengindikasikan potensi penggunaan asam humat dalam menstimulus pertumbuhan dan status kesehatan ikan mas sehingga dapat digunakan dalam pengembangan formulasi pakan ikan. Kata kunci: gambut, asam humat, hematologi, pertumbuhan, ikan mas
Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil Rousdy, Diah Wulandari; Rahmawati, Rahmawati; Kurniatuhadi, Rikhsan
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7499

Abstract

Peat soil is a type of soil that dominates the island of Borneo. Typical compounds in peat soil is humic acid. Various in vitro studies performed have shown peat subtropical humic compounds can stimulate the immune system. However, in vivo study on animal has not been done. This study aimed to determine the effect of humic acid extracted from peat soil of Borneo against the immune system, both of non-specific and specific immunity Wistar rats (Rattus novergicus). Research using a completely randomized design with five treatments and five replicates, the normal controls, a positive control (isoprinosine), humic acid 125; 250; 500 mg/kg. Humic acid was administered orally for 10 days. The results showed humic acid adduction did not significantly affect levels of hemoglobin, erythrocytes and hematocrit. Humic acid adduction of 125 mg/kg significantly affects the total leukocyte count and differential leukocyte. Humic acid 125 mg/kg also showed increased phagocytic index better than normal controls. All humic acid treatments do not provide a significant effect on the total amount of antibody. The results of this study can be used for the development of Borneo tropical peat resources as natural imunostimulant.How to CiteRousdy, D. W., Rahmawati, R. & Kurniatuhadi, R. (2016). Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil. Biosaintifika: Journal of Biology & Biology Education, 8(3), 401-406.
AKTIVITAS NEFROPROTEKTIF EKSTRAK METANOL BUAH LAKUM (Cayratia trifolia (L.) Domin) TERHADAP INDUKSI PARASETAMOL Kurniadi, Edi; Rousdy, Diah Wulandari; Yanti, Ari Hepi
Jurnal Labora Medika Vol 2, No 1 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

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Abstract

Parasetamol merupakan obat yang umum digunakan sebagai antipiretik dan analgesik tetapi dapat bersifat nefrotoksik apabila digunakan secara berlebih (overdosis). Buah lakum (Cayratia trifolia [L.] Domin) diketahui dapat digunakan sebagai nefroprotektif akibat parasetamol karena mengandung senyawa metabolit sekunder yang bersifat antioksidan. Tujuan dari penelitian ini adalah untuk mengetahui dosis optimal ekstrak metanol buah lakum (C. trifolia [L.] Domin) yang mampu meregenerasi nefron pada mikroanatomi ginjal tikus putih (Rattus norvegicus) yang diinduksi parasetamol. Rancangan penelitian pada penelitian ini adalah Rancangan Acak Kelompok (RAK) yang terbagi menjadi tujuh kelompok perlakuan, yaitu kontrol normal (akuabides), kontrol negatif (parasetamol), kontrol positif (obat HEPA-Q), kontrol pelarut (CMC 0,5%) dan kelompok ekstrak (115 mg/200 g BB tikus, 230 mg/200 g BB tikus dan 345 mg/200 g BB tikus). Hasil penelitian ini adalah ekstrak buah lakum dosis 230 mg/200 g BB tikus memiliki kemampuan regenerasi nefron yang optimal dengan rerata kerusakan glomerulus dan tubulus masing-masing 1,6 ± 0,54 dan 1,4 ± 0,44. Struktur mikroanatomi ginjal tikus pada kelompok ekstrak buah lakum dosis 230 mg/200 g BB tikus memiliki struktur yang tidak berbeda dengan kelompok positif.
Angka Lempeng Total (ALT) Bakteri pada Daging Ayam Broiler yang Dijual di Kota Pontianak Prianti, Prianti; Rahmawati, Rahmawai; Rousdy, Diah Wulandari
Jurnal Labora Medika Vol 2, No 2 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

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Abstract

Ayam broiler merupakan komoditas pangan yang memiliki nilai gizi yang tinggi dan mudah didapatkan, baik itu di pasar swalayan atau pasar tradisional. Penelitian ini bertujuan untuk mengetahui jumlah koloni bakteri pada daging ayam broiler yang dijual di pasar swalayan Kota Pontianak berdasarkan perhitungan Angka Lempeng Total (ALT). Sampel penelitian diambil dari tiga pasar swalayan di Kota Pontianak. Hasil pengamatan angka lempeng total bakteri dari 3 pasar swalayan yang terdapat di Pontianak melebihi ambang batas cemaran mikroba menurut SNI Tahun 2009 yaitu 1 x 106 cfu/ml, dengan hasil pengamatan sebagai berikut swalayan A berjumlah 3,2x106 cfu/ml, swalayan B berjumlah B 6,7 x 106 cfu/ml dan swalayan C berjumlah 3,8 x 106 cfu/ml.
HISTOLOGI LIMPA DAN HEMATOLOGI MENCIT YANG DIINFEKSI Escherichia coli SETELAH PEMBERIAN ASAM HUMAT GAMBUT KALIMANTAN Rousdy, Diah Wulandari; Wardoyo, Elvi Rusmiyanto Pancaning
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1465.233 KB) | DOI: 10.29122/jbbi.v5i2.2900

Abstract

Spleen Histology and Hematology of Mice Infected by Escherichia coli after Oral Administration of Humic Acid from Borneo PeatABSTRACTHumic acid compounds have an immunostimulatory effect. The aim of this research was to determine the effect of humic acid on the spleen of mice infected with Escherichia coli. The study used a completely randomized design with six treatments and five replicates. The treatments were normal control, negative control, positive control of isoprinosine, humic acid dose of 62.5; 125; and 250 mg/kg body weight (BW). The results showed that E. coli infection caused diarrhea symptoms and significant weight loss. There were significant differences (P<0.05) on hematocrit value and a total leukocyte count of humic acid, in which isoprinosine treatment was higher than those of negative control and normal control. There was no significant difference in the spleen weight of the mice subjected to the different treatments, but through histologic observations a significant difference (P<0.05) was found in the histologic size of the spleen. Humic acid treatment of 250 and 125 mg/kg BW resulted in the widest white pulp (495.8 ± 58.2 µm) and the highest leukocytes count (6725 ± 1018 cell/mL), respectively. On the red pulp serving as negative control numerous clusters of lymphocyte cells were found.Keywords: Escherichia coli, humic acid, peat soil, spleen, white pulp ABSTRAKSenyawa asam humat mempunyai potensi imunostimulan. Penelitian ini bertujuan mengetahui pengaruh pemberian asam humat terhadap organ limpa mencit yang diinfeksi bakteri Escherichia coli. Penelitian ini menggunakan rancangan acak lengkap dengan enam perlakuan dan lima ulangan. Perlakuan tersebut yakni kontrol normal, kontrol negatif, kontrol positif isoprinosin, asam humat dosis 62,5; 125; dan 250 mg/kg berat badan (BB). Hasil penelitian menunjukkan bahwa infeksi bakteri E. coli pada mencit menyebabkan mencit mengalami gejala diare dan penurunan berat badan yang signifikan. Perbedaan signifikan (P<0,05) pada nilai hematokrit dan jumlah leukosit total perlakuan asam humat dan isoprinosin lebih tinggi dibandingkan kontrol negatif dan kontrol normal. Tidak terdapat perbedaan nyata (P>0,05) pada berat limpa mencit antar perlakuan, melalui pengamatan histologi ditemukan perbedaan ukuran histologi limpa mencit. Perlakuan asam humat 250 mg/kg BB mempunyai ukuran pulpa putih (495,8 ± 58,2µm) dan perlakuan asam humat 125 mg/kg BB mempunyai nilai leukosit tertinggi (6725 ± 1018 sel/mL). Pada pulpa merah perlakuan kontrol negatif ditemukan banyak sel limfosit yang menggerombol.Kata Kunci: asam humat, Escherichia coli, limpa, pulpa putih, tanah gambut
HISTOLOGI LIMPA DAN HEMATOLOGI MENCIT YANG DIINFEKSI Escherichia coli SETELAH PEMBERIAN ASAM HUMAT GAMBUT KALIMANTAN Rousdy, Diah Wulandari; Wardoyo, Elvi Rusmiyanto Pancaning
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v5i2.2900

Abstract

Spleen Histology and Hematology of Mice Infected by Escherichia coli after Oral Administration of Humic Acid from Borneo PeatABSTRACTHumic acid compounds have an immunostimulatory effect. The aim of this research was to determine the effect of humic acid on the spleen of mice infected with Escherichia coli. The study used a completely randomized design with six treatments and five replicates. The treatments were normal control, negative control, positive control of isoprinosine, humic acid dose of 62.5; 125; and 250 mg/kg body weight (BW). The results showed that E. coli infection caused diarrhea symptoms and significant weight loss. There were significant differences (P&lt;0.05) on hematocrit value and a total leukocyte count of humic acid, in which isoprinosine treatment was higher than those of negative control and normal control. There was no significant difference in the spleen weight of the mice subjected to the different treatments, but through histologic observations a significant difference (P&lt;0.05) was found in the histologic size of the spleen. Humic acid treatment of 250 and 125 mg/kg BW resulted in the widest white pulp (495.8 ± 58.2 µm) and the highest leukocytes count (6725 ± 1018 cell/mL), respectively. On the red pulp serving as negative control numerous clusters of lymphocyte cells were found.Keywords: Escherichia coli, humic acid, peat soil, spleen, white pulp ABSTRAKSenyawa asam humat mempunyai potensi imunostimulan. Penelitian ini bertujuan mengetahui pengaruh pemberian asam humat terhadap organ limpa mencit yang diinfeksi bakteri Escherichia coli. Penelitian ini menggunakan rancangan acak lengkap dengan enam perlakuan dan lima ulangan. Perlakuan tersebut yakni kontrol normal, kontrol negatif, kontrol positif isoprinosin, asam humat dosis 62,5; 125; dan 250 mg/kg berat badan (BB). Hasil penelitian menunjukkan bahwa infeksi bakteri E. coli pada mencit menyebabkan mencit mengalami gejala diare dan penurunan berat badan yang signifikan. Perbedaan signifikan (P&lt;0,05) pada nilai hematokrit dan jumlah leukosit total perlakuan asam humat dan isoprinosin lebih tinggi dibandingkan kontrol negatif dan kontrol normal. Tidak terdapat perbedaan nyata (P&gt;0,05) pada berat limpa mencit antar perlakuan, melalui pengamatan histologi ditemukan perbedaan ukuran histologi limpa mencit. Perlakuan asam humat 250 mg/kg BB mempunyai ukuran pulpa putih (495,8 ± 58,2µm) dan perlakuan asam humat 125 mg/kg BB mempunyai nilai leukosit tertinggi (6725 ± 1018 sel/mL). Pada pulpa merah perlakuan kontrol negatif ditemukan banyak sel limfosit yang menggerombol.Kata Kunci: asam humat, Escherichia coli, limpa, pulpa putih, tanah gambut
MIKROANATOMI LIMPA TIKUS PUTIH (Rattus novergicus L.)SETELAH PEMBERIAN ASAM HUMAT DARI TANAH GAMBUT KALIMANTAN Wulandari Rousdy, Diah; Rahmawati; Kurniatuhadi, Rikhsan; Kurniadi, Edi
JURNAL BORNEO AKCAYA Vol 4 No 1 (2017): Jurnal Penelitian dan Pengembangan Pelayanan Publik
Publisher : Badan Penelitian dan Pengembangan Provinsi Kalimantan Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51266/borneoakcaya.v4i1.80

Abstract

Peat soil is organic soil types that has various biological potential to be developed. Humic acid compounds in the peat soil have a variety of potential biological one as immunostimulant. The aimed of this study to determine the effect of humic acid extracted from peat soil spleen histology Kalimantan against white male rats (Rattus novergicus L.) strain Wistar. Peat soil samples were taken in Pontianak on sapris maturity level. Humic acid is separated from other humic substances using IHSS methode. This research was done by completely randomized design with 4 treatments ie positive control (isoprinosin 380,3 mg/kg), humic acid 125; 250; 500 mg / kg body weight. Humic acid was administrated by oral gavage for 10 days. After 10 days, spleen organ was taken to preparat by paraffin methode and hematoxilin-eosin staining. Humic acid administration of 125 mg/kg showed a larger diameter than the white pulp isoprinosin control. However, humic acid administration of 500 mg/kg caused the excess stimulation of white pulp of the spleen congestion symptoms characterized by disarrangenment of lymphocytes cluster. There are no significant different of spleen weight between all treatments
Efek Paparan Musik Klasik, Hard Rock dan Murottal Terhadap Pertumbuhan Vegetatif Tanaman Bayam Merah (Alternanthera amoena Voss) Diah Wulandari Rousdy, Resti Elvi Rusmiyanto PW
Jurnal Protobiont Vol 7, No 3 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i3.29063

Abstract

This research was conducted to determine the effect of classical music, hard rock and murottal against to vegetative growing of red spinach plants. The research used a completely randomizes design with 4 treatments and 5 replicates. ANOVA result showed that music exposure had significant effect on plant growth. Murottal exposure gave optimal result on plant height 35,70 cm, leaf area 43,40 cm2, root length  9,40 cm, stomata porous length  23,00 µm, wet weigh 15,59 g, and dry weight of the plant 11,25 g. Exposure to hard rock music gives optimal results on the amount of leaf chlorophyll is worth 34,52 spad unit.
EFEKTIVITAS Pseudomonas sp. BOT4 DALAM MENDEGRADASI MINYAK JELANTAH MENGGUNAKAN SUMBER NITROGEN NATRIUM NITRAT DAN YEAST EXTRACT Harfan, Donatus Tia; Rousdy, Diah Wulandari; Kurniatuhadi, Rikhsan
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36872

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Jelantah is the residual waste of cooking oil. The disposal of untreated waste directly into the environment has the potential to promote damage such as water channels clogging and water body pollution. Form of waste treatment such as biodegradation can be done using potential bacteria such as Pseudomonas spp. which has been known for being effectively in decomposing organic waste. This study aimed to observe the ability of Pseudomonas sp. BOT4 in degrading jelantah with different nitrogen sources i.e. NaNO3 and yeast extract. This study was carried out from August to October 2018. The used cooking oil samples were homemade with deep frying method and the isolate samples were collected from used cooking oil-contaminated sewer water. Split plot design was used with time of incubation as main plots and nitrogen sources as subplots. The parameters observed were cell density and degraded oil weight. The results obtained stated that nitrogen sources of NaNO3 and yeast extract given optimum effect on cell density of Pseudomonas sp. BOT4 on day three each with OD600 value of 1,361 and 2,300. Nevertheless both nitrogen sources did not really give real effect on final weight of degraded oil, each with weight of 1,28 g dan 1,09 g.
Aktivitas Ekstrak Metanol Daun Akasia (Acacia mangium Wild) Terhadap Perkecambahan Dan Pertumbuhan Maman Ungu (Cleome rutidospermaeD.C) Dan Rumput Grinting (Cynodon dactylon L. Pers) Diah Wulandari Rousdy, Sumi Riza linda
Jurnal Protobiont Vol 7, No 3 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i3.29850

Abstract

Acacia (Acacia mangium Wild.) have alelokimia compound that can suppress the growth of the plants that it can be used as bioherbicide. This aimto study determined the concentration of the methanol extract of leaf of acacia capable of inhibit the germination and growth of weed, purple maman (Cleome rutidospermae DC.) and grass grinting(Cynodon dactylon L.). This study use a completely randomized design (CRD) with five replication and five treatment consist of control, concentrationmethanolextract0.2; 0.4; 0.6 and 0.8 g mlˉ1.Resultshowed that the methanol extract of leafacacia with a concentration of 0.4 g mlˉ1able to inhibit germinationpurple maman and grass grinting. Concentration of 0.2 g mlˉ1extract acacia can inhibit the growth of plant height, root length, and a decrease in wet weight and dry weight in weed purple maman andgrass grinting