cover
Contact Name
Elvi Rusmiyanto PW
Contact Email
elvi.rusmiyanto@fmipa.untan.ac.id
Phone
+6281522519199
Journal Mail Official
protobiont@untan.ac.id
Editorial Address
Jurusan Biologi, Fakultas MIPA Universitas Tanjungpura Jalan Prof. Hadari Nawawi Pontianak
Location
Kota pontianak,
Kalimantan barat
INDONESIA
Protobiont: Jurnal Elektronik Biologi
ISSN : -     EISSN : 23387874     DOI : http://dx.doi.org/10.26418/protobiont.
Jurnal Protobiont merupakan jurnal peer-reviewed dan open access journal yang mempublikasikan hasil-hasil penelitian biologi tropis yang berkaitan dengan bidang: 1. botani, 2. zoologi, 3. ekologi, 4. mikrobiologi, 5. genetika, 6. ilmu lingkungan, dan 7. biologi konservasi. Jurnal Protobiont terbit setahun 3 kali, yaotu bulan Maret, Juli, dan November. Jurnal Protobiont diterbitkan dan dikelola oleh Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura
Articles 358 Documents
Karakterisasi dan Kepadatan Bakteri Pendegradasi Selulosa pada Tanah Gambut di Desa Parit Banjar Kabupaten Pontianak Ahmad Mulyadi, Emma Khairiah, Siti Khotimah,
Jurnal Protobiont Vol 2, No 2 (2013)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v2i2.2748

Abstract

Selulosa merupakan salah satu bahan organik yang sukar untuk dirombak pada tanah gambut.Bakteri pendegradasi selulosa memiliki peranan dalam proses dekomposisi selulosa secara enzimatik dengan memutuskan ikatan rantai 1,4-?-glukosida. Penelitian ini bertujuan untuk mengetahui bakteri yang dapat mendegradasi selulosa dan mengetahui kepadatan bakteri pendegradasi selulosa pada tanah gambut. Sampel diambil di Desa Parit Banjar Kecamatan Mempawah Hilir Kabupaten Pontianak. Isolasi dilakukan dengan metode pengenceran cawan tuang dan cawan gores menggunakan media Carboxymethylcellulose (CMC) dan kepadatan bakteri dihitung dengan metode Total Plate Count. Pengamatan dan identifikasi dilakukan secara makroskopis, mikroskopis dan melalui rangkaian uji biokimia. Hasil isolasi diperoleh enam genera bakteri yaitu Acetobacter, Acinetobacter, Azotobacter, Acidomonas, Pseudomonas dan Cellvibrio. Kepadatan bakteri pendegradasi selulosa yang ada di lokasi penelitian berkisar antara 23,126x107 sampai 24,942x107 CFU/gr.
PENGARUH EKSTRAK BUNGA EKOR KUCING (Acalypha hispida Burm. F) TERHADAP PERTUMBUHAN Candida albicans (Y116) Hildayati, Umi; Wardoyo, Elvi Rusmiyanto Pancaning; Rahmawati, Rahmawati
Jurnal Protobiont Vol 9, No 2 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i2.45673

Abstract

Candida albicans is an opportunistic pathogenic yeast that causes the infection candidiasis. Ekor kucing flower (Acalypha hispida) is one of the plants that has a potential for antifungal activity. This research aims to determine the power of methanol extract of ekor kucing flower against the growth of C. albicans (Y116) and to discover the perfect concentration of methanol extract of ekor kucing flower to inhibit the growth of C. albicans. This study used the disk-diffusion agar method by utilizing the paper disc. This research is made up of 3 treatments  of methanol extract concentration of ekor kucing flower by 0 (DMSO 10%); 0.3; and 0.5 g/ml. The results of the study show that the concentration of methanol extract of ekor kucing flower offers an antifungal activity against the growth of C. albicans. Antifungal activity is represented with the result of a zone of inhibition. The ideal concentration inhibit the growth of C. albicans is 0.3 g/mL with inhibitory zone diameter 10.18 mm.
Pertumbuhan Anggrek Hitam (Coelogyne pandurata Lindl.) secara In Vitro Dengan Penambahan Ekstrak Tomat (Solanum lycopersicum L) Dan Benzyl Amino Purine (BAP) Riza Linda, Serliana, Mukarlina,
Jurnal Protobiont Vol 6, No 3 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i3.22500

Abstract

Black orchid (Coelogyne pandurata Lindl.) Is an endemic germplasm Kalimantan. This orchid is in great demand because of the potential to have high economic value. Consequently, it is often overexploited, yet it has not been widely cultivated. This research aims to find out the effect of augmenting tomato extract and benzyl amino purine (BAP) in the growing of black orchid. This research started from January to March 2017 in Tissue Culture Laboratory Aloe Vera Center (AVC) Pontianak. This research used Completely Randomized Design (CRD) with 2 treatment factors and 3 repetition. The first factor was tomato extract (0%; 7,5%; 10%; 12,5%) and second factor was BAP (0 M; 10-6 M; 10-7 M). The results show that the single factor of tomato extract and BAP and the combination factor also did not have a real effect on the time shoots appear and the number of leaves. The interaction factor of tomato extract and BAP did not have a real effect on the number of shoots, but the single factor of tomato extract and BAP had a real effect on the number of shoots and single factor of BAP had a real effect on the number of leaves. The highest number of shoots was 3,33 in the treatment of tomato extract 7.5% and 1.33 in the treatment of BAP of 10-6 M. The highest number of leaves was 11.33 sheets in the treatment of tomato extract of 12,5% and the lowest number of leaves was in the treatment of tomato extract of 10% and BAP of 10-6 M with an average of 3,00 sheets.
Inventarisasi Mikroalga dan Protozoa pada Instalansi Pengolahan Air Limbah Karet Sistem Biofilter Skala Laboratorium Ari Hepi Yanti, Marsi Orina Opat Tri Rima Setyawati
Jurnal Protobiont Vol 4, No 3 (2015)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v4i3.13241

Abstract

The treatment of liquid waste from rubber can be done with a biofilter method, which is by using micro-organisms attached to the filter to help the process of organic material degradation. The changes that occur to the organic substance in the waste will affect the composition of microalgae and protozoa during the treatment process. The purposes of this research is to find out types of microalgae and protozoa, their abundance in the rubber waste water treatment plant, and the quality of waste during the treatment. The research was conducted from September 2014 until January 2015. The research was conducted on the laboratory scale through observation of the microalgae and protozoa growth. The findings indicates that there are 36 genera of microalgae and 7 genera of protozoa. It shows that the highest abundance in the group of microalgae belong to Chlorophyceae class, whereas protozoa group is in the Sarcodina class. The genus which is able to adapt well would survive during the waste treatment processing. The genus which survived during waste  treatment process from the microalgae group was Cyanidium and from the protozoa group was Arcella.
Pertumbuhan Jagung (Zea mays L). dengan Pemberian Glomus aggregatum dan Biofertilizer pada Tanah Bekas Penambangan Emas Mukarlina, Desi Leskona, Riza Linda,
Jurnal Protobiont Vol 2, No 3 (2013)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v2i3.3890

Abstract

Gold mining activity caused damage to the soil as soil microbial populations, flora and fauna destruction, mercury content and tailling.using biofertilizer and mychorrizal are needed for corn growth ( Zea mays L). The research study was conducted from July 2012 to August 2012 used Complete Randomized Design (CRP) with two factor. The first factor was Glomus aggregatum with four level treatments; 0 g/polybag, 10 g/polybag, 20 g/polybag and 30 g/polybag. The second factor was biofertilizer with four level treatments that are; 0 ml/plant, 16 ml/plant, 32 ml/plant and 48 ml/plant. Each treatment was repeated three times with 48 unit experiments. The research finding showed that Glomus aggregatum 20 g/polybag gave the highest tall for plant height (69. 41 cm), fresh plant weight ( 51. 39 g), and dried plant weight (7.14 g). Giving biofertilizer 48 ml/plant produced the highest number of leaves (11.00 leaves). The interaction between biofertilizer 48 ml/plant and Glomus aggregatum 30 g/polybag showed the highest infection (7.14 %).
Variasi Morfometrik dan Pengelompokan Spesies Kantong Semar (Nepenthes Spp.) di Desa Simpang Kasturi Kecamatan Mandor Riza Linda, Anastasia Selviana, Masnur Turnip,
Jurnal Protobiont Vol 7, No 2 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i2.25295

Abstract

Nepenthes spp. is one of the ornamental plants in Indonesia that has much uniquenesses on its pitcher cup in shape and colour. The purpose of this research is to recognize the variety of morphometric characters and to know the grouping of Kantong Semar species (Nepenthes spp.) in Simpang Kasturi Village, Mandor District. The study was conducted for 3 months from October to December 2017 at Simpang Kasturi Village Forest, Mandor District. Identification, data processing, and the making of herbarium were done in Biology Laboratory of Biology Department, Mathematics and Natural Science Faculty of Tanjungpura University, Pontianak. The research method was done using the survey method following by sampling processes performed by taking samples of leaves, stems, and pitcher cup. Samples then were observed based on morphological characteristics of leaves, tendrils, stems and pitcher cup. Furthermore, collected data were analyzed using cluster analysis (grouping analysis) with SPSS 21 program. The results of this study found 5 individuals from 4 species of Nepenthes which have different morphometric characters among themselves. The most various morphological character among the species is the morphological character of the pitcher cup. Nepenthes rafflesiana has the largest pitcher cup size while Nepenthes gracilis has the smallest one size. Based on the coefficient value, the most significant grouping is Nepenthes rafflesiana with Nepenthes mirabilis, while the closest one is Nepenthes ampullaria 1 with Nepenthes ampullaria 2.
PERTUMBUHAN STEK PUCUK TANAMAN JABON PUTIH (Anthocephalus cadamba (Roxb.) Miq.) SETELAH DIRENDAM DALAM URIN KAMBING (Capra aegagrus) Mukarlina, Eka Widiana, Riza Linda,
Jurnal Protobiont Vol 5, No 1 (2016)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v5i1.14788

Abstract

Jabon (Anthocephalus sp.) is native species of Indonesia and the cultivation of white jabon in west Kalimantan is still rare. One of the efforts to increase the production of jabon is through vegetative propagation shoot cutting. Propagation through shoot cutting often fails, in which the roots cannot grow. One of the ways of overcoming such failure is by providing exogenous growth regulator, namely, goat urine. This research aimed to find out the effect of the concentration of goat urine (Capra aegagrus) and soaking time on the growth of the shoot cuttings of white jabon. This research used a completely randomized factorial design (CRD) i.e. the factor of concentration (A) consisting of 4 levels of 0%, 6%, 8%, 10%, 12% and the factor of soaking time (B) consisting of four levels, i.e. 0 min, 5 minutes, 10 minutes and 15 minutes. The treatment was repeated three times. The research findings indicated that adding goat urine concentration of 12% and 15 minutes soaking time generated the average root number of 29 pieces, the plant height of 20.33 cm, thewet weight of 16.33 grams and the dry weight of 3.46 grams, and the average number of leaves of 9 pieces, which were obtained from the treatment of a concentration of 10% and a soaking time of 15 minutes.
Kualitas Susu Cair Pasca Pasteurisasi Setelah Penambahan Sirup Oligofruktosa Umbi Talas Kimpul (Xanthosoma sagittifolium Schott.) Mukarlina, Mistia Ningsih, Tri Rima Setyawati,
Jurnal Protobiont Vol 3, No 2 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i2.5515

Abstract

One alternative of fresh milk preservation is by adding sugar that derived from the hydrolysis process of blue taro tuber. The aim of this study was to examine the quality of fresh milk after the addition of oligofructose syrup. The study was carried out from April to June in 2013. Based on the ANOVA test, it was known that the concentration of oligofructose syrup influenced the increase of proximately test, total number of bacteria, and reduction time of milk. The protein content of milk increased to 0.25% after being added by oligofructose syrup with 20% concentration, and then it became 3.95 %. The lactose content increased to 2.05% after being added by oligofructose syrup with 40% concentration, and then it become 6%. The highest increment of milk acidity occurred over milk by adding 40% of oligofructose syrup. Furthermore, 40% milk fat and oligofructose syrup decreased to 2.40%, which it became 2%. Total number of milk bacteria decreased to 23x103 CFU/ml after being added by 40% oligofructose syrup, but then it become 26x103 CFU/ml. In this study, no indication of coliform was found in all milk samples. Best time of reducing bacteria occured over milk added 40% syrup is 634 minutes.
Patogenitas Isolat Jamur Entomopatogenik Metarhizium anisopliae terhadap Lalat Rumah Musca domestica L. (Diptera: Muscidae) Kustiati, Noni Yunizar Rahmawati
Jurnal Protobiont Vol 7, No 3 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i3.29090

Abstract

Metarhizium anisopliae is one of the entomopathogenic fungi that can be used to control house fly, Musca domestica. The aim of this study is to determine the effectiveness of fungus Metarhizium anisopliae in killing house flies. This study used a Completely Randomized Design (CRD) with 5 treatment concentration of Metarhizium anisopliae fungal suspension concentration of 1x106 conidia/mL, 3x106 conidia/mL, 5x106 conidia/mL, 7x106 conidia/mL, 9x106 conidia/mL with each repetition three times. The fungal pathogenicity was determined by the lethal time of 50% flies (LT50) for each concentration using Probit analysis. The result of this study showed that the time required to killing 50% of flies in consecutive consentrations was 7 days with concentrations ranging from 3x106 conidia/mL until 5x106 conidia/mL. In conclusion, the fungus Metarhizium anisopliae is effectively deadly pest house flies Musca domestica.
Keanekaragaman Genus Dan Pola Distribusi Semut (Formicidae) pada Areal Perkebunan Jabon Putih (Antocephaluscadamba (Roxb.) Miq.) di Desa Durian Kabupaten Kubu Raya Linda, Adonovan Wulandari
Jurnal Protobiont Vol 5, No 2 (2016)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v5i2.15938

Abstract

White jabon (Anthochepaluscadamba) has high prospects for plantation forest and reforestation plants. Woody plants like white jabon (A. cadamba) are commonly used by ants for nesting. This research aims to find out the diversity of genera and distribution patterns of ants in the plantation area of white jabon (A. cadamba) in Sungai Durian Village, Sungai AmbawangSubdistrict. Data were collected through combined methods of transect and plot line in two sampling locations in the plantation area of white jabon (A. cadamba). The first location is a newly cleared land with jabon plants aged more or less 12 months. The second location is a previously horticultural farmland that was converted into agricultural farmland of jabon aged more or less 8 months. Samples of ants were collected with pitfall traps, bait traps, soil core sample and hand collecting. The results of sampling found 4 genera of ants i.e. Iridomyrmex, Solenopsis, DiacammaandMonomorium. Genus Iridomyrmex was a genus with most individuals (983 individuals) andMonomoriumsp was a genus with the fewest individuals (296 individuals). The index value of species diversity at both locations was low (Location 1 H '= 1.376 and Location 2 H' = 0.6843). The genus which dominates was not found and the condition of both locations was stable. Sorensen similarity index showed that both locations had a high similar genus (IS = 66.67%). All the ants had a clustered distribution patterns.