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Jurnal Ilmu dan Teknologi Kelautan Tropis

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Published by Institut Pertanian Bogor

ISSN : 20879423 EISSN : 2620309X DOI : https://doi.org/10.29244/jitkt

Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

Aims and Scope Journal of Tropical Marine Science and Technology (Jurnal Ilmu dan Teknologi Kelautan Tropis) is a scientific journal in the field of tropical marine science and technology. We have a aims and scope to focus in publishing a good quality scientific articles for dissemination of research results in the field of marine science and technology. Aims As an media of information and dissemination of research results in the field of marine science and technology, especially in the waters of Indonesia and Southeast Asia region, Could actively and continuously disseminate the best research results to various stakeholders, and; This Journal of Tropical Marine Science and Technology can improve the quality of research results and benefit stakeholders. Scope The article, published in this Journal of Tropical Marine Science and Technology covers a wide range of research topics in the field of: marine biology, marine ecology, biological oceanography, chemical oceanography, physical oceanography, dynamical oceanography, coral reef ecology, marine acoustic, marine remote sensing, marine geographical information system, marine microbiology, marine polution, marine aquaculture, post-harvest fisheries technology, integrated coastal management (ICM) marine biotechnology, air-sea interaction, ocean engineering,

Arjuna Subject : Umum - Umum

Articles 656 Documents

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IMPROVEMENT METHOD OF GENE TRANSFER IN Kappaphycus alvarezii St. Hidayah Triana; . Alimuddin; Utut Widyastuti; . Suharsono; Emma Suryati; Andi Parenrengi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Method of foreign gene transfer in red seaweed Kappaphycus alvarezii has been reported, however, li-mited number of transgenic F0 (broodstock) was obtained. This study was conducted to improve the method of gene transfer mediated by Agrobacterium tumefaciens in order to obtain high percentage of K. alvarezii transgenic. Superoxide dismutase gene from Melastoma malabatrichum (MmCu/Zn-SOD) was used as model towards increasing adaptability of K. alvarezii to environmental stress. The treat-ments were the culture media and recovery duration, and each treatment consisted of three replica-tions. The best method was co-cultivation using liquid media, then recovery was conducted in liquid media for 10 days. That treatment allowed higher transformation percentage (90%), regeneration effi-ciency (90%), putative bud efficiency (100%), number of buds and explants sprouted (100%) and transgenic explants (100%). The transgenic explants showed an amplification PCR product of Mm-Cu/Zn-SOD gene fragment, whereas the non-transgenic explants showed no amplification product. All results revealed that suitable method of transgenesis for K. alvarezii has been developed. Keywords: Agrobacterium tumefaciens, culture media, Kappaphycus alvarezii, recovery duration, transformation

POTENTIAL USE OF Ulva lactuca AS FEED INGREDIENT FOR TILAPIA Nur Hikma Mahasu; Dedi Jusadi; Mia Setiawati; I Nyoman Adi Asmara Giri
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Due to the mainly imported for fish feed ingredients in Indonesia, many attemps have been made to discover local potential ingredients to reduce the utilization of imported ingredients. In this experiment, the utilization of Ulva lactuca as feed ingredient for tilapia was evaluated. Experiment 1 was conducted to determine the digestibility of Ulva for tilapia. In experiment 2 and 3, Ulva was used to substitute wheat pollard of either 0, 3, 6, 9, or 12 % in the feed formulation. In experiment 2, fish with an average body weight of 4.1±0.15 g were fed on those diets, and were cultured for 55 days. To calculate the digestibity in experiment 3, Cr2O3 was added into the diet of exeriment 2; then fed on the fish with an average body weight of 19.0±0.67 g. Results showed that apparent digestibility of dry matter and crude protein of Ulva were 66.3 and 83 %, respectively. Ash content in the diet increased of 13.5% with Ulva inclusion of 12%. On the other hand, using Ulva as a substitute for pollard up to 12% was not affected the growth performance of fish. Regardless of the Ulva level in the diet, the digestibility of the diet was the same. Therefore, Ulva is a potential source of local feed ingredient for tilapia. Keywords: Ulva lactuca, tilapia, digestibility, growth

SPATIAL AND TEMPORAL VARIATION OF INDONESIAN THROUGHFLOW IN THE MAKASSAR STRAIT Agus S. Atmadipoera; Selfrida M. Horhoruw; Mulia Purba; Dwi Y. Nugroho
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Using outputs of INDESO model, this study investigated vertical structure, spatial and temporal variation of the Indonesian Throughflow in Makassar Strait (M-ITF). It was shown that the main axis of persistent southward jet of M-ITF formed a unique path following the western shelf slope along the strait, which was associated with a high kinetic energy (KE) region from near-surface down to the thermocline layer. Furthermore, a drastic jump of KE appeared in the narrow and deep Libani Chan-nel (near 3°S) where the strait's width shrinks significantly, thus an elevated flow velocity was needed to maintain transport volume balance. Here, maximum southward velocity at thermocline exceeded 1.2 m/s. Spatial pattern of M-ITF can be described by the first EOF mode which accounts for 79 % of the total variances. It exhibited that contours of the flow amplitudes were similar to M-ITF path, and the largest amplitude was located near the Libani Channel. Out-of-phase relationship of the flow was found between M-ITF and eddies circulation that developed in the edges of the strait. Corresponding temporal fluctuation of the first EOF mode indicated that M-ITF variabilities varied from intra-seasonal to inter-annual scales. Annual fluctuation of M-ITF was seen from EOF mode-2 (at thermocline layer) and mode-3 at lower-thermocline. Cross-spectra analysis revealed that variability of M- ITF (e.g. on annual scale) at northern entrance was highly coherent to the fluctuations of North Equatorial Current (NEC) and Mindanao Current (MC), suggesting that variability of M-ITF was remotely influenced by the Pacific low-latitude western boundary currents. Keywords: INDESO model, Indonesian Throughflow, Makassar Strait, EOF, Cross-Spectra Analysis

PLANKTON COMMUNITY STRUCTURE OF TRADITIONAL AND INTENSIVE BRACKISHWATER PONDS IN PROBOLINGGO REGENCY, EAST JAVA PROVINCE . Utojo; Akhmad Mustafa
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

This experiment aimed to determine the plankton community structure in intensive and traditional ponds of Probolinggo Regency, East Java Province. Plankton collected with plankton net size of 60 μm and preserved with lugol 1%. Water qualities such as temperature, transparancy, salinity, dis-solved oxygen, pH, total organic matter, NO2-N, NO3-N, NH3-N, PO4-P, and total suspended solids were measured and analysed in this study. Planktons were identified using microscopy. Cells were counted using cell counting method. The results showed that in intensive pond we found 16 species of phytoplankton and 7 species of zooplankton with abundance 570-1.808 ind./L, while in traditional ponds, we found 10 species of phytoplankton and 3 species of zooplankton with abundance 134-776 ind./L. The dominant species of phytoplankton in the intensive and traditional ponds were Navicula sp andNitzschia sp, each of Bacillariophyceae class with abundance of 423 ind./L and 198 ind./L, res-pectively. Zooplankton species in intensive pond was copepod sp, while in traditional one was Oitho-na sp, each of Crustaceae class with abundance of 66 ind./L and 37 ind./L, respectively. Diversity and abundance of plankton in intensive pond was higher than in the traditional ponds. The water quality in intensive pond was optimal so that the response to the absorption of N and P inorganic by phyto-plankton was quicker and more effective than in traditional pond producing an increase in plankton communities. The increase value of NO3-N and total suspended solids in intensive pond caused the in-crease abundance of Navicula sp. The high value of NH3-N caused the abundance of Navicula sp to decline. The high values of dissolved oxygen, pH, total suspended solid, and NH3-N in traditional pond can decrease the Nitzschia sp abundance. Keywords: plankton communities, intensive and traditional ponds, Probolinggo, East Java

THE EFFECT OF STOCK DENSITY OF GOLDEN-MOUTH TURBAN (Turbo chysostomus, L.) ON THEIR SURVIVAL AND GROWTH UNDER AIR WATER LIFT SYSTEM M.S. Hamzah
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Golden-mouth turban (Turbo chrysostumus, L.) belongs to the phylum of molluscs that inhabits coral reef ecosystem in group. Golden-mouth turban normally uses algae for their food. Study on survival and growth of the juvenile golden-mouth turban focussing on different density under air water lift sys-tem is still limited. This study was conducted on 7 January – 6 May, 2015, in a laboratory to observe the effect of different density on the survival and growth of golden-mouth turban under air water lift system. Based on the analyses of variance (ANOVA), there was no significant different (P>0.05) on mortality rate for different stock density treatment (5 ind., 10 ind., 15 ind., and 20 ind. each in 10 liter water volume). However, based on growth rate, the density of 5 individuals produced the highest monthly growth rate of 1.88 mm/month of shell height, 1.18 mm/month of mouth opening, and 1.1 gr/month of wet weight with the highest daily mean food absorption of 91.55 %. For all treatment, the correlation of shell height and wet body weight exhibited a similar growth pattrern i.e., allometry minor (b<3). Ketwords: Survival, growth, golden-mouth turban (Turbo chrysostumus), stocking density, air water lift system.

BINARY VECTOR CONSTRUCTION OF KAPPA(κ)-CARRAGEENASE GENE AND TRANSFORMATION TO Agrobacterium tumefaciens AS MEDIATOR FOR SEAWEED TRANSGENIC GENERATION Muh Alias L. Rajamuddin; . Alimuddin; Utut Widyastuti; Enang Harris; Emma Suryati
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Increasing of kappa (κ)-carrageenan content in Kappaphycus alvarezii seaweed is potentially be achieved by applying transgenesis technology. This study was performed to obtain a construction of κ-Carrageenase gene and Agrobacterium tumefaciens to carry those construction genes. The κ-Carrageenase (κ-Car) gene was involved in κ-carrageenan biosynthesis. The κ-Car gene sequence was ligated between the 35S CaMV promoter and tNos terminator sequences to generate pMSH/κ-Car expression vector. Transformation of pMSH/κ-Car plasmid to Escherichia coli was performed by heat-shock method, and to Agrobacterium tumefaciens by tri-parental mating method. The results showed that several colonies of E. coli and A. tumefaciens grew in the selective culture mediums containing antibiotic. PCR analysis using primers 35S-Forward and tNos-Reverse with DNA template from those bacterial colonies resulted DNA fragment of about 2,000 bp, the same as the total length of 35S CaMV promoter, κ-Car gene and tNos terminator sequences. Therefore, the construction of pMSH/κ-Car gene was succeeded and a colony of A. tumefaciens transformant carrying pMSH/κ-Car plasmid was successfully produced. Keywords: Agrobacterium tumefaciens, kappa(κ)-Carrageenase gene, transgenesis, vector

TOTAL HEMOCYTES, GLUCOSE HEMOLYMPH, AND PRODUCTION PERFORMANCE OF SPINY LOBSTER Panulirus homarus CULTURED IN THE INDIVIDUAL COMPARTMENTS SYSTEM Rifqah Pratiwi; Eddy Supriyono; . Widanarni
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Cannibalism can cause high mortality in a lobster culture. To prevent cannibalism, an artificial shel-ter is needed like the individual compartments system (ICS) to ensure that there is no contact bet-ween lobsters. This study aimed to evaluate the application of ICS on total hemocytes glucose hemo-lymph, production performance, and determine the shape of more effective ICS to minimize stress level of lobster. The treatments used for various application shape of ICS was tubular ICS, triangle ICS, square ICS, and shelter PVC pipes as control. Lobster maintenance was conducted in outdoor for 60 days. The results indicated that physiological responses with square ICS treatment were more effective to reduce stress level compared with other treatments (p<0.05). Application of ICS was more optimal to improved SR (p<0.05) and growth of lobster (p>0.05). Overall, treatment of square ICS was opti-mal related to production performance i.e. SR 88.89±5.24%, SGR 0.61±0.49%/day, with harvest size of total length 137.31±8.11 mm/ind, weight 58.83±4.78 g/ind, and FCR 22.71±1.72. Application of ICS in lobster culture was effective to reduce stress level as indicated from total hemocytes and gluco-se hemolymph, and support optimal production performance. Lobster culture using a square ICS sha-pe was effective to minimize stress level, compared with tubular ICS and triangle ICS.Keywords: cannibalism, glucose, hemocytes, individual compartments system, lobster, production

TURBULENT MIXING PROCESSES IN LABANI CHANNEL, THE MAKASSAR STRAIT Yuli Naulita
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Study on turbulent mixing processes in Labani Channel, the Makassar Strait, was conducted by using the INSTANT (International Nusantara Stratification And Transport) program dataset, in Juli 2005. The turbulent mixing process was evaluated using Thorpe method, where the overturning eddies were revealed by density inversions in CTD (Conductivity Temperature Depth) profiles. All individual identified-overturn regions was validated by the GK’s test (Galbraith and Kelly test) where at first noise on CTD signals had been removed by applying wavelet denoising. A large number of overturn regions with Thorpe scale (LT) less than 0.5 m were detected in the thermocline layer of Makassar Strait. Based on linear relationship between Thorpe and Ozmidov scale, order of magnitude of the turbulent energy kinetic dissipation rate in Labani Channel was estimated about 10-11- 10-5Wkg-1 and density eddy diffusivity Kρ(10-6 – 10-2) m2/s . The strong of turbulen mixing was found at the layer of NPSW at 150 m depth and NPIW at 300 m depth, indicated by high values of Kρ (O = 10-3 – 10-2 m2s-1). It reveals that turbulent mixing has an important role on determining ITF water mass character. Keywoods: turbulent mixing, wavelet denoising, overturn region, Thorpe method, Labani Channel, Makassar Srait.

MARINE TOURISM SUSTAINABILITY DEVELOPMENT IN MARINE RECREATIONAL PARK ANAMBAS ISLAND Rika Kurniawan; Fredinan Yulianda; Handoko Adi Susanto
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Mantang Besar and Mantang Kecil islands contain marine resources that can be developed for ma-rine tourism activities. For the above purpose, a study is needed to assess the potential of the islands. The objectives of this study were: (1) to analyze the sustainability potential of marine tourism on Man-tang Besar and Mantang Kecil islands and (2) to determine the policy and strategy to develop marine tourism in Mantang Besar and Mantang Kecil islands. Multi-Criteria Analysis (MCA) with Rapid Appraisal Index Sustainability of Ecotourism approach was used for the sustainability analysis of the marine tourism potential in both islands. The results showed that the sustainability status of marine tourism potential on Mantang Besar and Mantang Kecil islands for A dimension (ecology) was in ave-rage of 50.4592 within the category of sustainable, for B dimension (socio-economic) with average of 31.6113 within the category of less sustainable, and C dimension (institutional-technology) with ave-rage of 32.5581 wihtin the category of less sustainable. For the succes of the strategy development, B dimension should be prioritized and followed by B and C dimensions. Keywords: MCA, marine tourism development strategy, Anambas islands

COMMUNITY STRUCTURE ECHINODERMS (ASTEROIDEA, OPHIUROIDEA, ECHINOIDEA AND HOLOTHUROIDEA) IN THE MARINE NATIONAL PARK EAST SULAWESI WAKATOBI Eddy Yusron
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

A study on Echinoderms community structure in marine national parks of Wakatobi, Southeast Sulawesi was conducted in six locations, i.e., Waha Beach, Coastal Sombo, Beach Houses, Beach Kapota, Banakawa beach, and Umala beach in October 2013. All of the six parks were located the Wakatobi territorial waters with coordinates of 5°06'25" S and 123°124'10 E. The results showed of 18 species of echinoderms representing six different types of Asteroidea, two types of Ophiuroidea, six types of Echinoidea, and four types of Holothuroidea. Group of starfish or Asteroidea was the most prominent on seagrass area. Based on the six transects sites, it turned out that the group of starfish (Asteridea) occupied a relatively high level of species richness. From the quantitative analysis values, we obtained diversity index (H) of 1.105 in Sombu, the highest evenness index (J) of 0.989 was found in Umala, and the highest species richness index values (D) of 0.132 was obtained in kapota. It seemed that all echinoderm groups were generally like seagrass microhabitat (12 types). While, sand and dead coral habitats were only occupied by 8 (eight) echinoderm groups. Keywords: echinoderms, diversity, Wakatobi, Southeast Sulawesi

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Contact Name Dietriech G. Bengen

Contact Email dieter@indo.net.id

Phone +62251-8627323

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Editorial Address Departement of Marine Science and Technology Faculty of fisheries and marine science, IPB University Jln. Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680, Indonesia

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Contact Name
Dietriech G. Bengen

Contact Email
dieter@indo.net.id

Phone
+62251-8627323

Journal Mail Official
dieter@indo.net.id

Editorial Address
Departement of Marine Science and Technology Faculty of fisheries and marine science, IPB University Jln. Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680, Indonesia

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Jawa barat

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