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Farhan Baehaki
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Asian Journal of Health and Applied Sciences
Published by Lighthouse Publishing Company
ISSN : -     EISSN : 28298985     DOI : https://doi.org/10.53402/ajhas
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health
Asian Journal of Health and Applied Sciences (AJHAS) is an open access journal publishing high quality peer-reviewed articles across the breadth of health and applied sciences research. The journal takes an objective and constructive approach to peer-review, ensuring each manuscript is well evaluated on its own scholarly merits and research integrity. The journal considers research articles and literature reviews in the areas of medicine, nursing, midwifery, pharmacy, medical laboratory technology (hematology, clinical chemistry, immunoserology, bacteriology, clinical toxicology, immunohematology, cytotechnology, histotechnology), medical records, nutrition science, chemistry (biochemistry, health instrumentation, analytical chemistry, environmental chemistry, organic chemistry), biology (microbiology, molecular biology), occupational health and safety in the health sector, and public health.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)" : 5 Documents clear
Phylogenetic analysis of the SARS-CoV-2 virus gene-based on the chain A glycoprotein spike in Wuhan Pakpahan, Suyarta Efrida; Sari, Ira Prima; Koesmawati, Dea
Asian Journal of Health and Applied Sciences Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.266 KB) | DOI: 10.53402/ajhas.v1i2.6

Abstract

The spread of COVID-19 in various countries has increased the death toll due to COVID-19. Spike glycoproteins of the SARS-CoV-2 virus have an important role in binding to host cell receptors. This spike can identify the SARS-CoV-2 kinship in Wuhan and other countries by phylogenetic analysis. This study aims to determine the phylogenetic relationship of COVID-19 from Wuhan with the other countries obtained from the NCBI Gene bank based on spike chain A glycoproteins. The method uses NCBI's BLAST program to search for similar sequences, ClustalW's program to perform multiple alignment sequencing, and MegaX to create a phylogenetic tree. The research results Spike glycoprotein chain A in Wuhan has the closest kinship with the United States. This is indicated by the formation of tree branches close to each other but still in the same group as spike glycoproteins in other countries. Phylogenetic tree validated by the bootstrap test that value of 100%, which means it shows the sturdiness of the tree can be trusted. The conclusion is there is no significant difference in the characteristics of spike glycoprotein chain A, as for some countries that have spike amino acid differences in glycoprotein chain A, such as Pakistan, Poland, and Wuhan. The amino acid difference is considered normal because the virus will continue to evolve in order to adapt to the environment
Effect of lutein extract from spinach (Amaranthus sp) on n-hexane fraction on bulk cooking oil peroxide numbers Aryanie, Novia; Kurniasih, Nunung; Rosahdi, Tina Dewi; Junitasari, Assyifa
Asian Journal of Health and Applied Sciences Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.473 KB) | DOI: 10.53402/ajhas.v1i2.11

Abstract

The use of cooking oil for too long can cause rancidity in cooking oil which produces cancer-triggering free radicals. This rancidity can be overcome by adding antioxidants, namely compounds that can ward off free radicals. Lutein is an antioxidant compound that contains a hydroxyl group. In this study, green spinach leaves were used as a source of lutein. Green spinach leaves have a high lutein content that is not inferior to Kenikir and Broccoli flowers. The purpose of this study was to analyze the effect of adding lutein as an antioxidant compound in bulk cooking oil. The test begins with the maceration extraction stage using n-hexane solvent, determining the Rf value of lutein with TLC using an eluent mixture of n-hexane: acetone: chloroform (6: 2: 2) and analyzed by iodometric titration method. The results show that lutein has an Rf value of 0.58, which is almost the same as the standard Rf of lutein, which is 0.59, while the lutein content in green spinach leaves is quite high at 7.86 ppm. Lutein can inhibit the rancidity process caused by an increase in the number of peroxides. At a concentration of 3% lutein has a peroxide value smaller than 1% tocopherol which in this study was used as a comparison, namely synthetic antioxidant compounds.
Validity test of POCT (Point of Care Testing) method on blood glucose examination using whole blood samples, serum, and EDTA plasma Kafesa, Ally; Noviyanti, Santi; Nurdin, Nurdin; Sutomi, Muhammad Alwi
Asian Journal of Health and Applied Sciences Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (809.085 KB) | DOI: 10.53402/ajhas.v1i2.15

Abstract

Medical laboratory services can support disease diagnosis or monitor patient recovery. The reality of the POCT method has been widely used by clinical laboratories. The tool is not only used for screening but is also used to check the diagnosis of diabetes mellitus. This research aims to determine the validity of the POCT method on whole blood, serum, and plasma EDTA samples on blood glucose tests. The research method of this study used the descriptive-analytical method using the POCT method on blood glucose tests using three different types of samples, whole blood, serum, and plasma EDTA. Precision tests were accepted on normal and pathological serum samples with CV% of 2.02% and 2.27%. The accuracy test was accepted on a normal and pathological serum with TE% values ​​of 8.54% and 6.03%. The linearity test is accepted on serum–plasma EDTA samples with an r2 value of 0.998. The sigma values ​​are in the unacceptable area. The use of the POCT tool for blood glucose examination has a valid performance value. The deviation of the examination results is influenced by pre-analytical errors such as sampling and processing samples so that the total error obtained is higher than the total allowed error. The POCT tool can be used for all types of samples.
Peel-off mask formulation from bintangur (Calophyllum inophyllum L.) leaf ethanol extract and its antibacterial activity Liska, Liska; Amanah, Hairil; Novianti, Shindi; Mahardika, Robby Gus
Asian Journal of Health and Applied Sciences Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i2.29

Abstract

One of the most widely distributed plant species in Bangka Belitung is Bintangur (Calophyllum inophyllum L.) which is a genus of the Clusiaceae family. Bintagur leaf has the potential as a mask against acne-causing bacteria because it has high bioactivity such as antioxidants and antibacterials. Bitangur leaf extract masks were made by mixing the raw materials in the form of PVA 10 g; HPMC 1 g; glycerin 6 g; TEA 0.5 g; methylparaben 0.1 g; propylparaben 0.025 g; 30 ml distilled water and ethanol extract of Bintangur leaf formulation 0% 0 g; formulation 1% 0.5 g; 2% formulation 1 g and 3% formulation 1.5 g. Bintangur leaf ethanol extract mask in formulations F3 and F2 was the highest concentration producing an inhibition zone to inhibit the growth of Staphylococcus aureus and Propionibacterium acnes bacteria which were in the medium category and for formulations F1 and F3 the highest concentrations were to inhibit the growth of epidermal Staphylococcus bacteria which were categorized as The ethanol extract of Bintangur leaf whose antioxidant activity was measured obtained an IC50 value of 9.87 g/mL which was included in the very strong category. The mask of Bintangur leaf extract in the form of the peel-off gel has the potential to be used and marketed.
Mung beans (Phaseolus radiates L.): Utilization as components of the growth medium Bacillus subtilis Fadhilah, Wasiyah Khusna; Saputra, Heru Agung; Baby, Nimisha
Asian Journal of Health and Applied Sciences Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i2.35

Abstract

Bacillus subtilis is a bacterial contaminant widely discovered in laboratories and used in educational needs. Synthetic media are relatively expensive, which makes it difficult for microbiology laboratories to meet large-scale bacterial growth media demands. This has encouraged researchers to find alternative media with cheaper and easily available prices, namely Mung Beans, which are known to contain protein nutrients that are good for bacterial growth, which is as much as 24%. The purpose of this study was to determine the potential of mung beans’ alternative media as a medium for bacterial growth. This research is a descriptive study and the sample used is 1 tube of a pure isolate of Bacillus subtilis. The variable of this research is mung beans as an alternative medium for the growth of Bacillus subtilis. The results were obtained by observed macroscopically and microscopically, followed by a carbohydrate fermentation test and an IMViC test. Then showed that the colonies growing on the alternative medium of mung beans were in accordance with the characteristics of the colonies growing on Nutrient Agar medium (control), which indicated that these characteristics were Bacillus subtilis. Based on the research that has been done, it can be concluded that there is a qualitative growth of Bacillus subtilis in mung beans alternative media, which means that mung beans alternative media has the potential as a growth medium for Bacillus subtilis.

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