cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Ivandini Tribidasari A.
Contact Email
ivandini.tri@sci.ui.ac.id
Phone
+622129120943
Journal Mail Official
editor_mss@ui.ac.id
Editorial Address
Directorate of Research and Community Engagement UNIVERSITAS INDONESIA UI Campus, Depok 16424 Indonesia
Location
Kota depok,
Jawa barat
INDONESIA
Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol. 26, No. 3" : 7 Documents clear
Development and Optimization of an Immunoassay for the Detection of Antibodies Against SARS-CoV-2 with In-house Recombinant RBD Protein Ratu, Safira Pinaka Pramestika; Mariya, Silmi; Noviana, Rachmitasari; Saepuloh, Uus; Darusman, Huda Salahudin
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

COVID-19 caused by SARS-CoV-2 poses a major threat to the global community, particularly in Indonesia. Countermeasures to prevent the spread of this disease have also been implemented, including the implementation of a vaccination program. An immunoassay technique that can be used to analyze antibodies that might develop following vaccination is the indirect enzyme-linked immunosorbent assay (ELISA). We produced the recombinant spike protein used in this study. The optimization comprised adjusted concentrations of spike recombinant protein (5 and 10 ng/mL), blocking agent (2.5% and 5%), and conjugate (1:1000 and 1:5000). The optimal conditions in this study included a spiked concentration of 10 ng/mL, a blocking agent concentration of 5%, sample dilution of 1:33, and a conjugate concentration of 1:1000. The intra-assay value of this optimized indirect ELISA was 7.3, and the inter-assay value was 5.3. The commercial MyBioSource kit and immunodiagnostic were utilized as a reference in the T-test, with P-values of 0 and 0.313, indicating that the recombinant protein in-house ELISA kit in this study demonstrated the same ability as the commercial immunodiagnostic kit in detecting SARS-CoV-2 antibodies, allowing it to be used for post-vaccination efficacy evaluation.
Estimation of the Bubble Point Pressure of Multicomponent Reservoir Hydrocarbon Fluids Usen, Benjamin Sunday; Obi, Chidi
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study developed a novel C-sharp (C#) programming language for the estimation of bubble point pressure (BPP) of various hydrocarbon mixtures at equilibrium state. The methodology was based on vapor–liquid equilibrium calculation using Peng Robinson equation of state implementation, thermodynamic equilibrium calculation and Newton-Raphson’s method for the successive substitution of the unknown variables. The equal fugacity constraint can be satisfied by obtaining the equilibrium which serves as a criterion for two or more phases to exist at equilibrium. The problem was resolved by searching for a pressure that will satisfy the two constraints. Complex calculation was performed by successively substituting the pressure value estimated by Newton–Raphson’s method at reservoir temperature until the two constraints were satisfied. The BPP values for the eight reservoir sample fluids were within the range of 29.32–308.00 atm with an absolute error deviation ranging from 0.00–4.27 and average percentage error of 0.54%. BPP values were obtained were within the reservoir temperature range of 328.15–398.71 K. This procedure is a potential approach for the estimation of BPP for hydrocarbon mixtures with defined fluid composition irrespective of their composition.
Synthesis of Alginate/Hydroxyapatite Beads for Acetaminophen Delivery Angan, Anesylvia; Sambudi, Nonni Soraya
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Conventional drug delivery systems usually involve the intake of multiple drugs, which could amplify the risk of overdosing. Acetaminophen is a widely used pain relief substance that is prone to accidental overdosing. Hence, a controlled drug delivery system is needed to ensure its efficacy. A nanocomposite hydroxyapatite/alginate system that was used as a drug carrier for acetaminophen was synthesized through the sol-gel method, and the mechanism underlying its controlled drug delivery was investigated. Spherical nanocomposite bead samples were synthesized by incorporating 5 wt%, 10 wt%, and 15 wt% hydroxyapatite into mixtures containing alginate. The mixtures were then dropped into calcium chloride solution. The phase purity of the hydroxyapatite sample was confirmed through X-ray diffraction, and the functional groups that confirmed the presence of alginate and hydroxyapatite in the nanocomposite samples were analyzed by using Fourier transform infrared spectroscopy. The release of acetaminophen can be controlled for 48 h, and the Korsmeyer–Peppas kinetics model showed the best correlation for all samples. The kinetics of Al/HAp5, Al/HAp10, and Al/HAp15 were governed by quasi-Fickian diffusion with the n values of 0.199, 0.238, and 0.225, respectively. The composite beads show potential for application as a controlled drug delivery vehicle.
Comparative Photovoltaics of P3HT:N2200 and P3HT: Small-Gap Fullerene Ethyl-Nipecotate Bulk Heterojunction Structures Uddin, Md. Nasir; Islam, Rafiqul; Rahman, Muhibur; Chawdhury, Nazia
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This work deals with the study on the optical absorption and photoluminescence spectra of a p-type organic semiconducting polymer, regioregular poly 3-hexylthiophene-2,5-diyl (P3HT) and the absorption spectra of an n-type fullerene-based small-gap fullerene-ethyl nipecotate (Fullerene-EN) and a non-fullerene-based polynaphthalene bithiophene (N2200) semiconducting materials. The band gap of P3HT, N2200, and small-gap fullerene-ethyl nipecotate are 2.42, 1.65, and 1.51 eV, respectively, calculated from experimental results through the Tauc equation. Active layers with blends of P3HT and small-gap fullerene-ethyl nipecotate and P3HT and N2200 have been used to fabricate bulk heterojunction (BHJ) structures. The P3HT:N2200 BHJ structure follows the current density versus voltage characteristics of a photovoltaic device with 0.28% power conversion efficiency, 1.58 mA/cm2 short-circuit current density, and 41% fill factor. However, the P3HT:small-gap fullerene-ethyl nipecotate structure does not show any photovoltaic J–V characteristics.
Modulation of the NF-κB Activation Pathway by Phycocyanobilin from Spirulina platensis: An in Silico Study Iswanti, Febriana Catur; Purba, Hastuti Handayani S; Prijanti, Ani Retno; Fadilah, Fadilah; Herlina, Linda; Paramita, Reni
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Several studies have predicted the molecular interactions of the active ingredient of Spirulina platensis as an anti-inflammatory compound. However, these interaction studies did not review the modulation of the NF-κB activation pathway, which involves various factors. This study demonstrated the potential of the bioactive compounds of S. platensis for modulating immune function by reducing inflammation through the inhibition of the NF-κB activation pathway. Phycocyanobilin was predicted to have good potential for molecular docking with multisubunit IκB kinase (IKK)1/IKKA, IKK2/IKKB, NF-κB-inducing kinase, and the IκBα/NF-κB complex. Furthermore, β-carotene exhibited good potential for interactions with NF-κB essential modulator/IKK and the NF-κB complex, and α-glucan had the potential for interactions with COX-2. Therefore, supplementation with S. platensisand its bioactive compounds is expected to provide optimal benefits. Several studies have predicted the molecular interactions of the active ingredient of Spirulina platensis as an anti-inflammatory compound. However, these interaction studies did not review the modulation of the NF-κB activation pathway, which involves various factors. This study demonstrated the potential of the bioactive compounds of S. platensis for modulating immune function by reducing inflammation through the inhibition of the NF-κB activation pathway. Phycocyanobilin was predicted to have good potential for molecular docking with multisubunit IκB kinase (IKK)1/IKKA, IKK2/IKKB, NF-κB-inducing kinase, and the IκBα/NF-κB complex. Furthermore, β-carotene exhibited good potential for interactions with NF-κB essential modulator/IKK and the NF-κB complex, and α-glucan had the potential for interactions with COX-2. Therefore, supplementation with S. platensis and its bioactive compounds is expected to provide optimal benefits.
Mutation of Aspergillus sp. using Ultraviolet Light and Nitrous Acid for Amylase Production from Banana Peels Oshoma, Cyprian Erumiseli; Akor, Johnson Oche; Ikhajiagbe, Beckley; Ikenebomeh, Marcel James
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Strain improvement through mutagenesis is important in the commercial development of microbial fermentation. This study aimed to produce amylase from banana (Musa sapientum) peels by using mutant Aspergillus sp. strains obtained via ultraviolet (UV) radiation and nitrous acid-based mutagenesis. Results revealed that banana peels pretreated with 0.8 N HCl had a biomass yield of 3.02 g/L and amylase activity of 2.81 U/L. Of the UV mutants, strain AUV1 had the highest biomass and amylase activity of 4.50 ± 0.21 g/L and 3.46 ± 0.14 U/L, respectively. Subsequently, HNO2 mutants showed that strain ANA6 had 30.64% and 60.85% higher amylase activity than AUV1 and the wild strain, respectively. This study showed that banana peels can be utilized for amylase production. In addition, UV and HNO2 are effective mutagens for Aspergillus sp. strain improvement to enhance amylase productivity.
Metabolite Signature of Fresh and Long-term Stored Coffee Pulp and Husk Lestari, Wahyu; Hasballah, Kartini; Listiawan, Muhammad Yulianto; Sofia, Sofia
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Every product from the food and agriculture industry produces waste that can cause environmental pollution when carelessly discarded. Coffee husk and pulp are the main wastes generated by coffee processing. The secondary metabolites in these wastes can still be utilized, but their level can be affected by long storage. This research aims to determine the differences in the compounds obtained from old and fresh Gayo Arabica coffee pulp and husk. Coffee husk and pulp samples from Gayo Arabica coffee are extracted for GC-MS analysis to determine similarities between fresh and long-term stored Gayo Arabica coffee husk and pulp. Results show differences in level and type among the secondary metabolites. Among various compounds, caffeine is found in all the samples. The compounds obtained from Gayo Arabica coffee husk and pulp, such as caffeine, (Z,Z)-9,12-octadecadienoic acid, and palmitic acid, can be used in health and agriculture.

Page 1 of 1 | Total Record : 7

 1 

Filter by Year

2022 2022


Reset
Filter By Issues
All Issue Vol. 29, No. 3 Vol. 29, No. 2 Vol. 29, No. 1 Vol. 28, No. 4 Vol. 28, No. 3 Vol. 28, No. 2 Vol. 28, No. 1 Vol. 27, No. 4 Vol. 27, No. 3 Vol. 27, No. 2 Vol. 27, No. 1 Vol. 9, No. 1 Vol. 26, No. 4 Vol. 26, No. 3 Vol. 26, No. 2 Vol. 26, No. 1 Vol. 25, No. 4 Vol. 25, No. 3 Vol. 25, No. 2 Vol. 25, No. 1 Vol. 24, No. 4 Vol. 24, No. 3 Vol. 24, No. 2 Vol. 24, No. 1 Vol. 23, No. 4 Vol. 23, No. 3 Vol. 23, No. 2 Vol. 23, No. 1 Vol. 22, No. 4 Vol. 22, No. 3 Vol. 22, No. 2 Vol. 22, No. 1 Vol. 12, No. 1 Vol. 21, No. 4 Vol. 21, No. 3 Vol. 21, No. 2 Vol. 21, No. 1 Vol. 20, No. 4 Vol. 20, No. 3 Vol. 20, No. 2 Vol. 20, No. 1 Vol. 19, No. 4 Vol. 19, No. 3 Vol. 19, No. 2 Vol. 19, No. 1 Vol. 18, No. 4 Vol. 18, No. 3 Vol. 18, No. 2 Vol. 18, No. 1 Vol. 17, No. 3 Vol. 17, No. 2 Vol. 17, No. 1 Vol. 16, No. 3 Vol. 16, No. 2 Vol. 16, No. 1 Vol. 15, No. 2 Vol. 15, No. 1 Vol. 14, No. 2 Vol. 14, No. 1 Vol. 13, No. 2 Vol. 13, No. 1 Vol. 12, No. 2 Vol. 11, No. 2 Vol. 11, No. 1 Vol. 10, No. 2 Vol. 10, No. 1 Vol. 9, No. 2 Vol. 8, No. 3 Vol. 8, No. 2 Vol. 8, No. 1 Vol. 7, No. 3 Vol. 7, No. 2 Vol. 7, No. 1 Vol. 6, No. 3 Vol. 6, No. 2 Vol. 6, No. 1 More Issue