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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
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Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 11 Documents
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Search results for , issue "Vol. 8, No. 1" : 11 Documents clear
Construction and Characterization of Conductometric Biosensor for Determination of the Diazinon Concentration Prayoga, Indrajid; Mulyasuryani, Ani; Prasetyawan, Sasangka
Makara Journal of Science Vol. 8, No. 1
Publisher : UI Scholars Hub

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Abstract

Excessive diazinon residue in vegetables can endanger human health. Therefore, a simple, fast, and accurate method is needed to detect residue. A conductometric biosensor is a good choice because it also offers high selectivity and sensitivity. The principle of detection of the conductometric biosensor is based on enzymatic hydrolysis of diazinon into O,O diethyl phosphorothiate,2-isopropyl-6-methylpyrimidin-4-ol, and H+ catalyzed by organophosphate hydrolase (OPH). The optimum amount of organophosphate hydrolase added to the screen-printed carbon electrode (SPCE) modified with BSA-glutaraldehyde is 118.5 µg, while the optimum pH is 8.5. This biosensor has a response time of 30 sec, a linear dynamic range of 0 to 1 ppm, sensitivity of 42.21 µS/ppm, and limit of detection of 0.19 ppm.

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