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Jurnal Natural
ISSN : 14118513     EISSN : 25414062     DOI : https://doi.org/10.24815/jn
Jurnal Natural (JN) aims to publish original research results and reviews on sciences and mathematics. Jurnal Natural (JN) encompasses a broad range of research topics in chemistry, pharmacy, biology, physics, mathematics, statistics, informatic and electronic.
Articles 7 Documents
Search results for , issue "Volume 25 Number 1, February 2025" : 7 Documents clear
The validation of urea determination in saliva using optical urea biosensor with p-dimethylaminobenzaldehyde (DMAB) reagent ALDIANSYAH, TEUKU; SAFITRI, EKA; GINTING, BINAWATI; NAZARUDDIN, NAZARUDDIN; SUHUD, KHAIRI; SALEHA, SITTI; NURHAYATI, NURHAYATI; NAZARUDDIN, SALSABILLA LATANSA; AFIFI, MUHAMMAD RIDHO; NURHAIDA, NURHAIDA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.41251

Abstract

The validation of urea determination in saliva using the biosensor method and the p-dimethylaminobenzaldehyde (DMAB) method has been successfully conducted. Anthocyanin compounds from Chatarantus roseus flowers were extracted using the maceration method with methanol as the solvent, yielding a total of 22.60% with a total concentration of 6.01 mg/L. The obtained extract was subjected to qualitative anthocyanin testing, showing a positive result was indicated by the formation of a reddish color and quantitative testing with the formation of a faded yellow color. The maximum wavelength ( max) of anthocyanin was 664 nm with an absorbance of 0.674. The saliva samples were obtained from three different ages i.e: children, teenagers, and adults. During the saliva collection procedure, participants were instructed to abstain from consuming any food or beverages, with the exception of water, for a minimum of 1 hour prior to sample collection. All samples were stored in a freezer at 4-8C until needed for analysis. Linearity test results were assessed using a calibration curve, yielding a coefficient of determination (R) of 0.978 with a sensitivity of 0.033 for the biosensor and an R of 0.975 with a sensitivity of 0.685 for DMAB. The limit of detection (LOD) values were determined as 7.203 10 M for the biosensor and 6.984 10 M for DMAB, while the limit of quantification (LOQ) values were 2.182 10 M and 2.116 10 M, respectively. Statistical analysis using a t-test showed tcalculated = 1.314, compared to ttable = 4.302, indicating that tcalculated ttable. This result suggests no statistically significant difference between the biosensor and DMAB methods, confirming that the biosensor method is comparable in performance to the DMAB method. Additionally, urea concentration measurements showed that adult samples exhibited the highest urea levels among the tested samples, which may indicate an association with potential health risks, including dental caries, kidney failure, and liver damage.
Assessing the water quality in the Timbalun river watershed, Padang City, West Sumatra, Indonesia HAR, RUSLI; TANJUNG, DENNY AKBAR; JAMARUN, NOVESAR
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.42009

Abstract

The purpose of this study is to assess water quality, specifically pH, conductivity, total dissolved solids (TDS), salinity, and turbidity. The research utilizes quantitative methods and covers 15 sampling points across the Timbalun River basin in Padang City, West Sumatra, Indonesia. Samples were measured based on several parameters, with three repetitions for accuracy. The testing took place from August to September 2024. The results showed that some water quality parameters, such as pH and total dissolved solids, met the standards outlined by Ministry of Health Regulation No. 2 of 2023. However, other parameters, including salinity, conductivity, and turbidity, did not meet the specified thresholds. It is essential to implement treatment measures to ensure the water meets the required standards for its intended use.
The role of Pediococcus pentosaceus IL13 in the fermentation of tuna waste, Euthynnus affinis RAMADHAN, ZULFA AULIA; SUNARTI, TITI CANDRA; MERYANDINI, ANJA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.43091

Abstract

Processing tuna fish (Euthynnus affinis) produces wastes like heads, tails, and viscera, which can pollute the environment. However, this waste is nutrient-rich and can serve as a source of peptones, antimicrobials, and antioxidants. Align with Sustainable Development Goals (SDGs) through the zero-waste concept, which emphasizes reducing waste production, this study aims to analyze the role of Pediococcus pentosaceusIL13 in the fermentation of tuna fish waste. The fermentation process was conducted over 30 days using two types of fermentation, spontaneously and with the addition of P. pentosaceus IL13 culture. Bacterial dynamics, pH, protein content, and amino acid concentration were monitored to assess fermentation performance. The study found a reduction in total bacteria and lactic acid bacteria (LAB) along with an increase in pH in both types of fermentation over time. Maximum protein and free amino acid levels were observed on day 10. In the spontaneous fermentation, protein concentrations were 5.590.15% in the liquid phase and 20.051.19% in the solid phase. In the fermentation with added culture, protein concentrations were 6.590.57% in the liquid phase and 19.560.00% in the solid phase. Amino acid concentrations were 4821.11149.3 ppm for spontaneous fermentation and 6271.11377.1 ppm for fermentation with culture. The protein hydrolysates in the liquid phase have antibacterial activity against S. typhi.
The measurement of microbial air contamination index in the pulmonary ward, intensive care unit and pediatric intensive care unit at dr. Zainoel Abidin hospital, Aceh, Indonesia RINANDA, TRISTIA; SAKDIAH, SAKDIAH; RISTIANTI, RIMA; YUNIRA, VONNA; VANIA, FEBY; BUANA, ANDIKA CITRA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.43797

Abstract

Nosocomial infections are one of the major problems in hospitals. Air is one source of nosocomial infections. Hospitals are filled with many patients, their families, and health workers daily. This large number of people allows the spread of various microorganisms through the air in droplets dispersed through breathing, talking, coughing or sneezing. This study used passive air sampling or settle plate method to measure the air quality in the pulmonary ward, Intensive Care Unit (ICU) and Pediatric Intensive Care Unit (PICU) at dr. Zainoel Abidin Hospital (RSUDZA). The air microbial contamination index was determined by counting the microbial colonies grown on Nutrient Agar and converted to CFU/m3 of air volume using the Polish Standard PN 89/Z-04008/08 Formula. The results showed that the air microbial contamination index in the pulmonary ward, ICU and PICU exceeded the maximum threshold mentioned in the decree of the Minister of Health of the Republic of Indonesia No.1204/SK/X/2004, which was 500 CFU/m3 for the pulmonary ward and 200 CFU/m3 for the Intensive Care Units. Although the findings of this study only represent the large microbial particles pulled by gravity, the high microbial contamination index of each unit trigger air quality alerts. Therefore, adequate air system management and periodic monitoring are needed to improve air quality in the wards and intensive care units at RSUDZA.
The effectiveness of naphthaleneacetic acid and kinetin on the development of plantlet book cuttings of Chrysanthemum ornamental plants BUDI, RAHMAD SETIA; PARAMUJI, MUJI; TANJUNG, DENNY AKBAR
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.42084

Abstract

The high demand for chrysanthemum flowers, coupled with the challenge of acquiring quality seeds in large quantities within a short timeframe, presents significant problems in this study. One potential solution is to propagate plants and utilize plant growth regulators to accelerate their development. Tissue culture techniques, which are essential in agriculture and biotechnology, are employed to produce new plants by isolating, developing, and duplicating specific plant cells or tissues in the laboratory. This study aims to assess the effects of two plant growth regulators Naphthalene Acetic Acid (NAA) and Kinetin on the growth and development of stem cuttings or nodes from ornamental chrysanthemum plants (chrysanthemum) in vitro using MS media. The research utilized a completely randomized design (CRD) factorial approach featuring two treatment factors: NAA and Kinetin. A total of nine treatment combinations were tested, with NAA concentrations of 0.5, 1.0, and 1.5 ml L-1, and Kinetin concentrations of 1, 2, and 3 ml L-1. Each treatment was repeated three times. Observations included measurements of plant height, leaf count, root count, and wet weight. The best results for each parameter were observed in treatment K3N2, which yielded a plant height of 30.73 mm and an average of 18.33 leaves. The optimal results for root count and wet weight were found in treatment K3N3, which produced an average of 13.83 roots and 3.57 grams, respectively. Overall, increasing the concentrations of NAA and Kinetin positively influenced all parameters evaluated.
Optimization of growth rate and feeding efficiency of four economically important in the bioflocs system HANAFIE, AGUSSYARIF; MURJANI, AKHMAD; REDHA, EL
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.41139

Abstract

Abstract. Research and development of biofloc aquaculture system until now only in the range of 14-15 species conducted. Even though this technology is able to increase production several times compared to conventional systems, so it is necessary to diversify species that allow development, especially economically important fish. There are 4 species of fish that are feasible to be developed, especially freshwater fish groups in wetlands, such as fish (climbing perch; Anabas testudineus Bloch, snakehead, Channa striata Bloch, giant gouramy , Opronemus gouramy and Pangas , Pangasius hypophthalmus. This research has been carried out in the range of June 2023- February 2024 using a complete randomised complete block design, 4 treatments climbing perch; snakehead, giant gouramy and pangas with 4 replicates. Stocking density of 1000 fish 2500 L-1 using 2 m diameter round pond with biofloc media; feeding twice a day and the amount of feed given was 2.5% of total weight, for 158 days. Research parameters included; daily growth rate (%/day), feed efficiency. FCR, weight gain (gr) and length gain (cm), water quality and biofloc content (ml/L). The results showed that the results of the anova test showed a significance value (Sig.) of 0.000 0.05, so there was a significant difference between treatments of the daily growth rate (SGR) parameter, the results of the area test also showed differences between treatments. The highest daily growth rate was obtained in climbing perch, followed by pangas, giant gouramy and Snakehead. The results of the anova test for weight gain showed a significance value (Sig.) of 0.000 0.05, so there was a significant difference between treatments. The anova test results showed a significance value (Sig.) of 0.052 0.005, so there was no significant difference between treatments; the growth of each treatment showed peculiarities between species depending on genes. Anova test results show that the feed conversion of fish research significance value 0.000 0.05 means that the four types of fish studied, significantly different from those reared in the biofloc system Survival rate of fish research significance value 0.000 0.05 means that the four types of fish studied, significantly different from those reared in the biofloc system. Water quality with 4 main parameters is able to support the growth and development of the 4 economically important fish species studied.
Bacterial patterns and antibiotic susceptibility among pus culture in dr. Zainoel Abidin General Hospital, Banda Aceh KEUMALAHAYATI, MEUTIA; HAYATI, ZINATUL; MAELANI, IMELDA; RIDWAN, AZWAR; RAHMAWATI, CUT VIKA; MULIANA, DESI; PUTRI, FARAH DHITA ANISSA; MAHDANI, WILDA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.44523

Abstract

The collection of pus samples is crucial for identifying serious bacterial infections. Accurate identification of the pathogen and selection of the appropriate antibiotics are essential for effective treatment. This study used a descriptive retrospective method to investigate pus specimens bacterial patterns and antibiotic susceptibility at dr. Zainoel Abidin General Hospital in Banda Aceh in 2023. Out of 1,274 examined pus specimens, 782 yielded positive cultures, revealing 61 bacterial species, predominantly Escherichia coli (22%), Staphylococcus aureus (16%), and Pseudomonas aeruginosa (9%). Gram-negative bacteria constituted 70.20% of the isolates. High antibiotic susceptibility rates were observed for Vancomycin (98%) and Linezolid (98%) in Gram-positive bacteria, while Amikacin (86%) and Meropenem (74.74%) were effective against Gram-negative bacteria. Notable resistance was found in Methicillin-resistant S. aureus (81%) and E. coli resistant to third-generation Cephalosporins (55%). These findings underscore the importance of monitoring bacterial patterns and antibiotic resistance for improved infection management at dr. Zainoel Abidin General Hospital, Banda Aceh.

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