Garuda - Garba Rujukan Digital

DYNAMICS OF DENGUE HEMORRHAGIC FEVER INCIDENCE WITH SPATIO-TEMPORAL APPROACH IN MARITENGNGAE SUBDISTRICT, SIDENRENG RAPPANG DISTRICT, SULAWESI SELATAN PROVINCE, IN 2008-2009 I Made Swastika; Tri Baskoro Tunggul Satoto; Anis Fuad
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Dengue Hemorrhagic Fever (DHF) causes 40% of world population at risk for infection, and 50 million people get infection with as many as 24,000 die every year. Incidence rate in Indonesia, particularly in Maritengngae Subdistrict, Sidenreng Rappang District, is increasing and needed to be controlled. Disease distribution has not been documented regionally. The dynamics of the incidence,that comprises the characteristics of the patient, environmental conditions, susceptibility of mosquitoes resulted from insecticide use, transovarial infectivity, and the incidence pattern were unknown.Objectives: To identify the dynamics of DHF incidence with spatio-temporal approach, includes gender, age, the habit of cleaning the water containers, house density, the density of water containers within the radius of 100 meters from the house of the patient, resistance of the mosquito, and transovarial transmission of Dengue virus.Methods: Subjects were DHF patients in Maritengngae Subdistrict. It was an epidemiological observational study with cross-sectional design and spatio-temporal approach to variables of the study, which included the patient characteristics (age, gender, habit of cleaning the water containers), environmental conditions (house density, water container density within 100 meter radius around the patient’s house), and vector conditions (resistance status to organophosphate insecticide, and transovarial transmission of Dengue virus).Results: Cases of DHF in Maritenggae Sub district, Sidenreng Rappang District, South Sulawesi decreased from 95 cases in 2008 to 38 cases in 2009, except in Kelurahan Majeling. Incidence distribution of DHF in 2008-2009 based on gender were not different (p=1.000), patients with age <15 years old were different from age ≥15 years (P=0.016 and p = 0.013), house density and density of water containers around the patient’s house were not different (p=0.829 and p = 0.538). The habit of cleaning water containers at the house was 43.84%. There were 1.94% of Aedes aegypti samples showed medium resistance (tolerance) status against organophosphat insecticide and 33.33% of samples showed transovarial transmission of Dengue virus. The highest of transovarial transmission occured in Kelurahan Majeling with transovarial transmission index of 100%.Conclusion: The transmission dynamic of DHF in Maritengngae sub district is not influenced by gender, house densities, water container densities, and the frequency of source reduction, but influenced by ages. Most of Ae. aegypti mosquitoes are still susceptible against organophosphate insecticides. The highest of transovarial transmission of dengue virus in Ae. aegypti in Kelurahan Majeling with Transovarial transmission index of 100% may contribute in the increasing of DHF cases in the Kelurahan Majeling in 2009.Keywords: dynamics, dengue, spatio-temporal, Ae. aegypti, Maritengngae.

Transovarial Transmission Index of Dengue Virus on Aedes aegypti and Aedes albopictus Mosquitoes in Malalayang District in Manado, North Sulawesi, Indonesia Angle Maria Hesti Sorisi; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Dengue Hemorrhagic Fever (DHF) is an infectious vector-borne disease caused by Aedes sp mosquitoes still cause serious health problem in Indonesia. Based on Manado Health Office Report, Malalayang was identified as dengue-endemic areas. In 2010, number of DHF cases in Malalayang is 211 cases with Incidence Rate (IR) 328 per 100,000 populations. Dengue viruses (DENV) survive in nature by two mechanisms; by horizontal transmission through infected vertebrates and mosquitoes, and by vertical (transovarial) transmission in the mosquitoes. Transovarial transmission is assumed as an important aspect in the maintanance of DENV during inter epidemic, but this problem has not been studied in Malalayang District, Manado. An effort to prevent and control DHF requires knowledge of an Aedes sp Dengue virus transovarial infection.Objectives: To prove the existence of Dengue virus transmission in Ae. aegypti and Ae. albopictus mosquitoes and its relationship with the incidence of DHF in Malalayang District in Manado, North Sulawesi, Indonesia.Methods: The method of this research was an observational analytic study with cross-sectional design. Study samples were unbloodfed Aedes aegypti and Aedes albopictus mosquitoes on the F1 generation from ovitrap placed in five selected villages based on the number of cases in the District Malalayang. The secondary data of DHF patients from Malalayang district was obtained from Health Office Manado and the Community Health Center in 2010. The presence of dengue antigen in head squashes preparation were detected using monoclonal antibody against dengue (DSSE10) based on immunohistochemical streptavidin biotin peroxidase complex (ISBPC) technique to confirm the presence of transovarial transmission of dengue virus both in Ae. Aegypti and Ae. Albopictus, and to obtain the data of transovarial transmission index. Fisher’s Exact test and Pearson correlation are used to analyze those data.Results: Transovarial transmission of Dengue virus in Aedes sp was found from 5 villages in Malalayang district with Transovarial Transmission Index (TTI) ranges 6.1%-17.1%. Statistic test showed significant differences in positive rate (p-value=0.00<0.05) on Ae. aegypti higher than Ae. albopictus. It is also known that there is no statistically significant correlation (p-value=0.528>0.05) between the Aedes sp. Dengue virus TTI and DHF IR in Malalayang district.Conclusion: This study demonstrates the existence of Dengue virus transovarial transmission in Aedes sp in Malalayang district. Ae. aegypti’s TTI is higher than that of Ae. Albopictus, and no significant correlation between TTI and DHF IR in Malalayang district.Keywords: DHF, transovarial transmission, Ae. aegypti, Ae. albopictus

THE KINETIC OF ACTIVATED MONOCYTES IN ACUTE PHASE DENGUE INFECTION Umi Solekhah Intansari; Arif Bahiyuddin bin Badaruddin; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introductions: Nowadays dengue infection is at present one of the most common mosquito-borne viral diseases of humans worldwide. Initially, Dengue infections were primarily recorded when they occurred as epidemics in tropical and subtropical countries. Monocyte/macrophage infection is central to the pathogenesis of dengue fever and to the origin of dengue hemorrhagic fever. Increased activation of monocytes and greater numbers of DEN-infected cells were associated with more severe Dengue, implicating a role for monocyte activation in dengue immunopathogenesis. Thus, more knowledge about the relation between numbers of activated monocyte with dengue severities is essential for better understanding regarding this subject.Objectives: Studying the relation between numbers of activated monocyte, marked by HLA-DR marker intensity with Dengue Fever severity throughout the disease course, from day 2 until day 6 of acute dengue fever.Methods: This research is cross sectional observational study. The location in done at Dr. Sardjito Hospital in 6 months time.Results: There is a negative correlation between median number of activated monocyte with dengue severity, on day 3 of acute dengue fever.Conclusion: There was a negative correlation between numbers of activated monocyte marked by HLADR intensity with disease severity on day 3 of acute dengue infection.Keywords: Activated Monocyte; Human Leukocyte Antigen (HLA)-DR intensity; Percentage of Activated Monocyte; Dengue Fever Severity.

The Relationship Between The Prevalence of Head Lice Infestation with Hygiene and Knowledge Among The Rural School Children In Yogyakarta Hemelamariae Munusamy; Elisabeth Elsa Herdiana Murhandarwati; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Pediculosis capitis causes considerable social distress. Rural children are in great risk because they live in conditions where poor knowledge and hygiene are related to this matter. However, the prevalence of pediculosis capitis among school age children in rural area in Yogyakarta have not known yet.Objectives: The objective of this study is to find the prevalence of head lice infestation among the rural school children and associating it with hygiene and knowledge.Methods: This was a cross sectional study done in February 2011 in 2 primary public schools in the rural areas of Yogyakarta with 158 subjects age ranging from 8 to 16 years old. Questionnaires inclusive of hygiene and knowledge aspect were given prior to the gross visual head lice examination by trained personnel.Results: The prevalence of head lice infestation among the rural school children is 19.6%. There is no significant relationship between this with knowledge and hygiene.Conclusion: The prevalence of head lice infestation in rural school areas in Yogyakarta is 19.6%. There is no significant relationship between the prevalence of head lice infestation with hygiene and knowledge among the rural school children in Yogyakarta.Keywords: Pediculosis capitis, head lice infestation, hygiene, knowledge

DEVELOPMENT OF A PROTOTYPE OF MALARIA CLINICAL DIAGNOSTIC DECISION SUPPORT SYSTEM Sudarta Yabesman Harefa; Lutfan Lazuardi; Anis Fuad
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction : Malaria is a public health problem that still causes mortality, particularly in high risk population. Kabupaten Nias is one of the malaria endemic areas. Malaria diagnosis is mainly determined according to physical examination, despite the fact that laboratory examination is the gold standard of malaria diagnosis. To help health workers in diagnosing malaria accurately, it is necessary to develop a decision support system for malaria diagnosis.Objectives: To develop a prototype of malaria diagnostic decision support system.Methods: It was a descriptive study with action research design to explore each phase in the development of a prototype of malaria diagnostic decision support system. Participants of the study consisted of 5 general practitioners in RSU Gunungsitoli and 2 nurses in Puskesmas Gunungsitoli.Results: The study created an application model of computer-based malaria diagnostic decision support system designed using PHP programming language and MySQL database. This system worked by entering malaria clinical symptoms into the expert system, and data of symptoms were processed by the expert system to determine diagnosis and medical advice that was useful to assist health staff in making decision.Conclusion: Malaria diagnostic decision support system that had been developed attracted the interest of health workers and help them in diagnosing malaria clinically.Keywords: diagnostic decision support system, prototype, malaria, computer application, Gunungsitoli

In vivo Antiplasmodial of the Most Active Fraction and Its Compound of Kapur Leaves (Harmsiopanax aculeatus Harms) Extract Against Plasmodium berghei Rachel Turalely; Ratna Asmah Susidarti; Mahardika Agus Wijayanti
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction : The rising of Plasmodium resistance towards chloroquine and other antimalarial drugs have encouraged to discover and develop new drugs mainly derived from natural products. Harmsiopanax aculeatus (kapur plant) has traditionally used by people of in Maluku Province to treat malaria.Objectives: The aims of this study were to identify antiplasmodial activity and its chemical constituents of the most active fraction of kapur leaves.Methods: The dried powder of Kapur leaves (1.3 kg) were extracted successively by maceration with n-hexane, ethyl acetate and methanol. After removal the solvents the hexane 15.6 g (1.2%), ethyl acetate 53.3 g (4.1%) and methanol 61.1 g (4.7%) extracts were obtained. Those extracts were assayed for their in vivo antiplasmodial activities by using 4-days suppressive test in Swiss mice infected with Plasmodium berghei, HPIA and identified the compound by GC-MS.Results: The ED50 of hexane, ethyl acetate and methanol extracts were 467.58, 2074.02 and 16.16 mg/kgBW, respectively. Fractionation of the methanol extract gave 18 combined fractions (FG1 – FG18). FG8 was the most active fraction with the IC50 HPIA of 18.22 μg/ml. Phytochemical test of this fraction using spray reagent showed the existence of essential oils, triterpenoids, and phenolic compounds. Separation of FG8using pressed chromatography gave 19 combined fractions (FG8.1-FG8.19). The fraction containing intense blue fluorescent spot (FG8.5) was further separated by PLC fourthly eluted with chloroform. Seven major components with the percentage of compotition more than 3.11% were identified as eugenol (tr = 12.692; 18.22%), isoprophyl myristate (tr = 16.333; 3.99%); bis(2-methylpropyl) phtalat (tr = 16.939; 7.15%); methyl palmitic (tr = 17.442; 3.11%); palmitic acid (tr = 17.883; 25.72%); butyl 2-methylpropyl phtalat (tr = 17.957; 9.37%) and bis(2-ethylhexyl) phtalat (tr = 23.258; 23%).Conclusion: Methanol extract of H. aculeatus was the most potential in vivo antiplasmodial activity. Combined fraction 8 which contain 7 compounds was the most active fraction.Keywords: Harmsiopanax aculeatus Harms, in vivo antiplasmodial, HPIA, PLC, GC-MS

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA