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JURNAL BIOLOGI PAPUA

JBP
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Published by Universitas Cenderawasih

ISSN : 25030450 EISSN : 20863314 DOI : 10.31957/jbp

Core Subject : Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology

JURNAL BIOLOGI PAPUA dengan nomor ISSN: 2503-0450 dan EISSN: 2086-3314 diterbitkan oleh Jurusan Biologi FMIPA Universitas Cenderawasih. Jurnal ini mempublikasikan tulisan ilmiah hasil penelitian asli maupun telaah pustaka (review) yang berhubungan dengan biologi secara umum. Penulis dianjurkan menuliskan karyanya dalam Bahasa Inggris, walaupun dalam Bahasa Indonesia tetap kami hargai. Jurnal ini terbit dua kali setahun setiap Bulan April dan Oktober.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 242 Documents

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Identifikasi Molekular Kekerabatan Genetik Kopi Wamena Berbasis Marka Random Amplified of Polymorphic DNA (RAPD) I Made Budi; Arsyam Mawardi
Jurnal Biologi Papua Vol 13, No 1 (2021)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Baliem Coffee or Wamena Arabica Coffee is classified as a specialty coffee that has a distinctive aroma and taste as one of the world's best coffees. Wamena coffee has a high commercial value, there are many advantages of Wamena coffee. Growing on a variety of soil types, elevation, coffee variety, and durable. The quality of Wamena coffee must be maintained by the local government. However, this elite coffee germplasm is threatened with extinction and is in a dangerous situation due to habitat destruction due to forest clearing, land use change and the potential for competition with various disease-resistant varieties. This study aims to identify molecularly the genetic relationship of Wamena Arabica coffee in Jayawijaya district with the Random Amplified Polymorphic DNA (RAPD) markers as molecular markers. The stages in this study were sample collection, morphological observations, genomic DNA extracted using the CTAB method, gene amplification process using PCR-RAPD molecular markers with ten primers, measurement of DNA concentration using a spectrophotometer, and data analysis using UPGMA NTSYS version 2.1. The electroferogram results showed that pRAPD1, pRAPD2 and pRAPD5 primers produced a band pattern with high polymorphism as well as indicating the genetic diversity of the Coffea arabica L. arabica coffee samples tested. Based on the dendogram construction analysis, the arabica coffee sample Coffea arabica L. which was tested on 5 coffee genotypes, obtained 2 coffee groups, namely Group 1 consisting of one variety, namely sample (V) Arabica Typica from the Assolokobal area. Group 2 with four varieties, namely samples (I) Arabica Catimor, (II) USDA, (III) Arabica PM 88, and (IV) Arabica Linies 795, from Wollo. Phylogenetic tree construction resulted in a similarity coefficient of 83%, indicating the high relationship between Arabica coffee populations. The results of this study have obtained a genetic fingerprint profile of wamena specialty arabica coffee, namely its genetic relationship. The superiority of the genetic composition of Wamena coffee in Jayawijaya district in the future has the potential to be derived through much more advanced molecular technology.

Studi Ekologi Moluska Bernilai Ekonomi Pada Hutan Mangrove di Teluk Youtefa Kota Jayapura Tenny Wijayanti; Suriani Br. Surbakti; Samuel J. Renyaan
Jurnal Biologi Papua Vol 5, No 2 (2013)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Study of ecology of mangrove organisms at Yotefa Bay Jayapura has been done from February to December 2012. The main purpose of this study is to collect the ecological data mangrove organism focus on diversity, distribution, richness, and similarity. The study was on organisms which have economical values for people living at the Papua Trade Centre area (PTC), Skyland, and Nafri. The data were collected with the line transects and quadrate methods, and data was examined with description on quantitative and qualitative methods. The result showed that there were 7 species of mangrove organism which have economical values; which were Anadara granosa, Anadara pilula, Isognomon ephippium, Gelonia erosa, Gelonia expansa, Pinna nobilis, and Faunus ater. The diversity index revealed that the highest number of species diversity was in PTC and Nafri area which were 1,70 and the lowest species diversity was in Skyland area which was 1, 55. The highest species evenness (E) was occurred in skyland and Nafri, which was 0,80 and the lowest was in PTC which was about 0,32. The highest abundance of species occurred in Skyland area was G. expansa scoring 4,9 ind/m2 and the lowest abundance in Nafri was P. nobilis scoring 1 ind/m2. The similarity index for the PTC Area, Skyland, and Nafri was on spesies of G. erosa, G. expansa, A. granosa, I. ephipium, and F. ater. Key words: Mollusca, mangrove, Youtefa bay, and Jayapura.

Biologi dan Ekologi Thiaridae (Moluska: Gastropoda) di Danau Sentani Papua Suriani Br. Surbakti
Jurnal Biologi Papua Vol 3, No 2 (2011)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Lake Sentani has been subjected to multi-pronged pressures, involving both internal and external factor. Yet, ecologists researching the lake generally, conduct studies on water quality, but less attention was paid towards faunal diversity and biological resources potentials of the lake. This research is focused on the diversity of Thiaridae species (Gastropoda). The research was conducted for two months from October to November 2009 using line transect methods in settlement and no settlement areas, inlet and outlet areas. The transects was installed in the lake from the shoreline at 0 to 0.5m depth and 0.5 to 1.0m depth and 1.0 to 1.5 m depth. At each depth sample, 4 points were taken. Transect at each location, repetition was repeated 4 times. The results brought in the diversity of Thiaridae as 7 species, namely Tiara scabra and Melanoides granifera, Melanoides tuberculata, Melanoides canalis, Melanoides copali, Melanoides sentaniensi, Stelomelania sp. The highest diversity were found in no settlement region with a value of H was 0.65 and the lowest were found in residential area with a H value was 0.57, inlet area was 0.59 and outlet area was 0.34. Density in no settlement areas was 0.36, inlet area was 1.84 and outlet area was 0.64. Environmental parameters was also assessed in this study, such as temperature, pH, DO, phosphorus, nitrite and nitrate. Key words: threat, Lake Sentani, diversity, density, Mollusc.

Karakterisasi Bakteri Endofit Tanaman Sirih Timor (Piper betle L.) Penghasil Antibakteri Gergonius Fallo; Lukas Pardosi; Anjelina M. da Cruz
Jurnal Biologi Papua Vol 14, No 2 (2022)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Indonesia has a wealth of various types of plants that are useful as traditional medicinal plants. One of the medicinal plants that are often used by Indonesian people, especially on the island of Timor, East Nusa Tenggara (NTT) Province, is betel plant (Piper betle L.). Isolation of endophytic bacteria using the scratch method and the scatter method. The results of the isolation obtained 9 isolates. The results of the characterization of 9 isolates had round colonies, small, medium, large, flat elevation, and clear white color. The results of the bacterial test showed that 3 isolates had the potential as antibacterial, namely: SDE01, SAE03 and SBE04 isolates. Of the three isolates, SBE04 isolates had inhibition zone diameters of 1.7 and 1.35 mm against Escherichia coli and Staphylococcus aureus.Key words: antibacterial; endophytic bacteria; characterization; P. betle.

Kemampuan Prospektif Fusan FP4 dalam Memproduksi Inulinase dan Profil Pertumbuhannya Wijanarka Wijanarka; Ika Anggraini
Jurnal Biologi Papua Vol 9, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Fusan FP4 is one type fused materials derived from intraspecific fusion of Pichia manshurca. The fused materials have superior capabilities compared with other materials, especially in producing inulinase enzyme (E.C. 3.2.1.7). The aims of the study are to determine the production of the enzyme inulinase of fusan FP4 and the growth profile formed. Growth can be detected by the addition of size and cell division. Growth measurements of fusan FP4 was carried out for 24 hours and observation and sampling was done every 6 hours for 24 hours. Observations of growth are conducted qualitatively by using a spectrophotometer, while the production of inulinase done using DNS method. The results of this study showed that the highest inulinase production was 1.948 U/ml and occurred at log phase with 6 hours of incubation time (t6) to 12 hours (t12). Key words: inulinase, Fusan FP4, log phase, P. manshurca.

Skrining Golongan Senyawa Kimia dan Pengujian Aktivitas Antioksidan Ekstrak Ubijalar (Ipomoea batatas (L.)Lam.) Varietas Lokal di Distrik Skanto Kabupaten Keerom Provinsi Papua Yuliana H. Rumsarwir; Linus Y. Chrystomo; Maklon Warpur
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Sweet potato (Ipomoea batatas) is native plant comes from the American continent. It began to spread throughout the world, especially in the tropical countries in the 16th century. Papua Province is one of the regions with the largest area of harvested sweetpotato in Indonesia, therefore there is a high chance to develop food independence in Papua. Because of the Papuan people are accustomed consuming non-rice food. The study was conducted in the Biology Laboratory and the Pharmacy laboratory of MIPA Faculty Cenderawasih University and also the supporting Laboratory of The Papua Agricultural Research and Development Center (Balitbangtan). The purpose of this research is to test the quality of flour of Keerom sweet potato varieties. Method for flour of sweet potato quality testing were using phytochemical screening to determine the chemical compound group and using antioxidant test with the DPPH (1-diphenyl-2-picrylhydrazyl) method. The results of the description of the Skanto District Keerom sweet potatoes in the field were three (3) local varieties including Weayuken (purple sweet potato), Musanaken (yellow sweet potato) and Hiho (white sweet potato). The result of phytochemical screening show that the purple sweet potato variety has more chemical compounds consist of tannins, quinones, flavonoids, terpenoids and alkaloids compared to white sweet potato consist of alkaloids only and yellow sweet potato consist of tannins, flavonoids, terpenoids and alkaloids. The result of antioxidant activity tests to flour extract ethanol has IC50values of 299.82 ppm (purple sweet potato), 301.18 (yellow sweet potato), 1027.98 ppm (white sweet potato) respectively. So can be conclude that the purple sweet potato variety is the best. It was suggested for further research to isolate bioactive compounds and test antioxidants to other varieties in other centers areas of sweetpotato culture which have potential to develop for food, supplement and tradisional drugs.Key words: Sweet potato; local varieties; flour quality; phytochemical screening; antioxidant test

Aktivitas Enzim Amilase Isolat Bakteri Amilolitik dari Tepung Sagu Basah dan Lingkungan Tempat Penyediaannya Secara Tradisional di Jayapura Suprapto Surapto; Tri Gunaedi; Basa T. Rumahorbo
Jurnal Biologi Papua Vol 6, No 2 (2014)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

The study about the activity of the enzyme amylase from amylolytic bacterial isolates from wet sagoo starch and its traditional provision environment had been done in Jayapura. The purposes of this study were to determine the activity of amylase enzyme and to identify the bacteria isolated from wet sagoo starch and its processing environment in Jayapura district. The method used was an experimental laboratorium in which isolation of amylolytic bacteria was performed by using nutrient agar medium with 1% soluble starch on spreed pour plate method. The enzyme activity was detected with 0.2% iodine in 2% potassium iodide which were able to form a clear zone. The protein content of the crude enzyme extract was determined by the Bradford method using bovine serum albumin (BSA). Amylase enzyme activity was determined by the formula: DUN/ml = [(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial isolates have amylolytic power of more than 30 mm. The amilase activity of amylolytic bacterial of all isolates were quite high: which were 35 577, 18 903, 32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The identification of isolates indicated that the three isolates are members of the Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%, and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value of 94.30%. Keywords: Amylolytic bacteria, amylase activity, characterization, sago flour.

Skrining Senyawa Kimia dan Uji Aktivitas Sitotoksik Ekstrak Etanol Teripang Kridou Bintik (Bohadschia argus Jeager) Asal Pantai Harlem Kabupaten Jayapura, Papua Apianus Kilungga; Linus Y. Chrystomo; Puguh Sujarta
Jurnal Biologi Papua Vol 11, No 1 (2019)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

The Bohadschia argus Jeager contains bioactive compounds that have the potential as antibiotics, antibacterial, antitumor, anticoagulants and anesthetics and alleviate cancer. This study aims to determine the content of chemical compounds group and to test the cytotoxic activity of ethanol extract of Bohadschia argus Jeager. The method for determining the group of chemical compounds was used the color reaction and precipitation using chemical reagents. The method for testing cytotoxic activity using the Brine Shimp Lethality Test (BSLT), this method commonly was used to test the safety of the use of natural ingredients as traditional medicine. The results of screening of the chemical compound group of Bohadschia argus Jeager extract showed that the extract contained a lot of alkaloids secondary metabolites and few saponins. The results of testing of cytotoxic activity showed LC50 value of 878.22 ppm. Base on the results of this study it can be concluded that the ethanol extract of Bohadschia argus Jeager has significant cytotoxic activity against Artemia salina Leach, so it can be considered as a chemotherapeutic agent. Key words: Screening, chemical compounds, ethanol extract, B. argus, BSLT.

Pemanfaatan Tumbuhan Obat Masyarakat Marind yang Bermukim di Taman Nasional Wasur, Merauke Dwi Haryanto; Rosye H.R. Tanjung; Konstantina M.B. Kameubun
Jurnal Biologi Papua Vol 1, No 2 (2009)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Study on the used of medicinal plants by Marind people who lived at Wasur National Park, Merauke was conducted by using descriptive methods which include observation, interview, documentation, literature review techniques, as well as emic and ethic approaches. During the study there were 46 species which belong to 26 families plants found as medicinal plants used by Marind people to cure about 30 diseases. Among 46 species, there were 7 potential medicinal plant species which known and used by most Marind people. The seven potential medicinal plant species were Ipomoea triloba L., Bauhinia sp., Pittosporum sp., Kingiodendron platycarpum Bent., Sophora tool mentosa L.Cyrtandra sp., dan Tinopspora disstiflora L. Part of plant used vary from leaf, root, bark, fruit and other part of plant. Compare to other part of plant, leaf was the most common used as traditional medicinal plant. Key words: traditional medicinal plant, Marind people, Wasur National Park

Senyawa Bioaktif Herpetofauna pada Penderita Diabetes Mellitus dan Hipertensi: Tinjauan Secara Patofisiologi Aditya K. Karim; Rury Eprilurahman; Laksmindra Fitria; Paul J. Kawatu
Jurnal Biologi Papua Vol 5, No 1 (2013)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Amphibians and reptiles (herpetofauna groups) produced of many compounds. The herpetofauna and their chemical compounds have been the subject of intense research interest for many years due to their potential in medical application and in lead compound development for new drugs. A number of different types of compounds have been identified from the glandular skin secretions of amphibians and venom or saliva of reptiles. These compounds have been shown to be active against gram-negative and gram-positive bacteria, fungi, enveloped viruses, diabetes mellitus, hypertension and cardiovascular disorder.Key words: Herpetofauna, diabetes mellitus, hypertension, drugs, pathophysiology

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Home Page

OAI Link

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Editorial Address Laboratory of Biology, 1nd Floor, Faculty of Mathemathic and Natural Sciences, Cenderawasih University. Jl. Kamp. Wolker UNCEN–WAENA, Jayapura–Papua. 99385

Location Kota jayapura, P a p u a INDONESIA

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Contact Name
Suharno

Contact Email
harn774@yahoo.com

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+6281344615035

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jbiolpapua@yahoo.com

Editorial Address
Laboratory of Biology, 1nd Floor, Faculty of Mathemathic and Natural Sciences, Cenderawasih University. Jl. Kamp. Wolker UNCEN–WAENA, Jayapura–Papua. 99385

Location
Kota jayapura,

P a p u a

INDONESIA