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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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Identifikasi Leptin setelah Luka Irisan padaTikus yang Diberi Pakan Lemak Tinggi Devita Anggraeni; Dhirgo Adji; Sitarina Widyarini
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Leptin is a hormone produced by adipocytes and plays an important role in wound healing. The objectives of this research were to identify leptin and total leukocyte after incision on rat fed high fat diet and zinc topicalapplication. Sixteen male Sprague Dawley rats at 3 months of age were used in this study. Rats were randomly allotted into 4 groups (A,B,C and D) of 4. Rats in group A and B were fed normal diet, while rats in group C and D were fed high fat diet. After 2 months of treatment, skin incision was done at the back side of the rat. Incision wound was then closed with single interrupted suture. The wound of the rats in group A and C was treated with vaseline, while in group B and D was treated with zinc 10%. Three days after surgery, blood was collected from each rat for leptin analysis and total leukocyte. Leptin level and total leukocyte were analyzed statistically using ANOVA for factorial experiment (2x2). The results showed that leptin level was significantly higher in rats fedhigh fat diet compared to that of the rats fed normal diet (P<0.05). There was no significant different on total leukocyte. Therefore, it was concluded that leptin level was affected by diet, while total leukocyte was notaffected neither by diet nor topical application. However, total leukocyte tends to be lowered in the rat with zinc topical application.Keywords: high fat diet, leptin, zinc, total leucocyte, Sprague Dawley rats

Pengaruh Penangkaran terhadap Profil Eritrosit Lumba-lumba Hidung Botol dari Perairan Laut Jawa Guntari Titik Mulyani; Yuda Heru Fibrianto; Teguh Budipitojo
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In the Java Sea, there are a lot of dolphins, that often snagged fishing nets. While In Indonesia, bottlenosedolphins are mammals protected water. The aims of this research was to study the erythrocytes profile inbottlenose dolphins before and after the captivity. Seven dolphins were used in this research. Blood samples weretaken out from the superficial vein of tail for examination of the number of erythrocytes, hemoglobin levels andvalue of hematokrit. Sampling was done when the dolphins appointed from the waters of the Java Sea and afterexperiencing dolphin captivity in PT. WSI, Kendal, Central Java. The data before and after captivity were compared and tested with the statistical analysis on the level of significance of the paired t-test 95%. The results showed the significant increase in the number of erythrocytes, hemoglobin levels and the values of hematokrit significantly. It was concluded that captivity done by WSI Corporation, Kendal, Central Java did increase the profile of erythrocytes in bottlenose dolphins.Keyword : Bottlenose dolphins, captivity, profile erythrocytes, superficial vein of tail, paired t-test

Studi Lesi Makroskopis dan Mikroskopis Embrio Ayam yang Diinfeksi Virus Newcastle Disease Isolat Lapang yang Virulen Hamdu Hamjaya Putra; Haryadi Wibowo; Tri Untari; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Newcastle disease (ND) is caused by Avian paramyxovirus, family Paramyxoviridae, one of the major diseases in chickens. This research was aimed to find lesions in chicken's embryo organs macroscopically andmicroscopically, infected by pathogenic ND virus. Embryonic chicken eggs (ECE) were inoculated by the ND Salatiga virus and ND La Sota virus as a control avirulent virus. Aquabidestilata used as a negative control. ECEwich showed the death of the embryos, stored in the refrigerator. The allantois fluid was collected, for further examination of viral growth. Chicken embryos that died then observed macroscopically. The organs of chicken embryos were made into histopathologic preparations stained with Hematoxylin and Eosin (H&E) for microscopic analysis. The identification of ND virus growth on isolates was done by haemagglutination andhaemagglutination inhibition test using an anti-ND serum. The chicken embryos that were infected by the ND Salatiga virus died approximately 26 hours post-inoculation. Macroscopic lesions were visible as haemorrhagein the skin. Microscopic lesions indicated the congestion and haemorrhage in lungs, inflammation and congestion in the skin, congestion in intestines, liver, kidneys and heart. There was also mild congestion on theskin in chicken embryos infected by ND La Sota virus. The microscopic lesions showed congestion in lungs, liver, kidneys and heart, also the inflammation and congestion on the skin. The macroscopic and microscopic lesions of chicken embryos infected by the ND Salatiga virus were more severe than lesions caused by ND La Sota virus.Key words: Newcastle disease, chicken embryos, macroscopic lesions, microscopic lesions, La Sota

Histopathologic Feature(s) of The Dactylogyrus sp-Infected Gills in The Ornamental Marine Fish Putu Eka Sudaryatma; Ni Nyoman Eriawati
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In the present study, Dactylogyrus sp. was examined in the gill(s) of the ornamental marine fish due to the fact that this kind of parasite is very often found in those fishes without any pathognomonic clinical signs orpathologic anantomic lesions. The gill(s) samples collection and examination were performed in the Laboratory of Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan. during Janury-July 2011. At first, the gills were examined natively and were subsequently processed for histopathologic examination. In the native examination, it was found the Dactylogyrus sp. with their size(s) ranging from 200-500 um. At necropsy, the gills were swollen and looked darker than normal. Based on the histopathologic lesions, the epithelial cells of the gills lamellas either primary or secondary became hyperplasia and fusion. The fusion of the gills is primarily thought to cause fatal to the fish due to significant oxygen depletion.Keywords: ornamental marine fish, gill, Dactylogyrus sp., native examination, hiperplasia and fusion.

Factors Associated with Occurrence of Campylobacteriosis on Village Chicken in Mataram Anwar Rosyidi; Setyawan Budiharta; Doddi Yudhabuntara
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Campylobacter jejuni is responsible for about 90% of cases of campylobacteriosis in human with gastroenteritis. Efforts to prevent the incidence of campylobacteriosis in human should be started with anassessment on its level in poultry. This study aims to determine the factors associated with the presence of C. jejuni on village chicken in Mataram, Nusa Tenggara barat. Association between C. jejuni as the dependent variable with independent variables were analyzed using logistic regression. Prevalence of C. jejuni from a total of 216 samples in Mataram at chicken level was 35.6% and while at the farm level was 70.5% (31/44). Variables showed a positive association of C. jejuni occurrence in chicken consist of age of chicken more than 3 months, the number of chicken ownership, and cage with soil floor. While the variable showed a negative association to the incidence of C. jejuni was drinking water of chicken originated from wells. Model obtained using logistic regression in association with occurence of C. jejuni on village chicken in Mataram was Y = -2.033 + 0.271 (age of chicken more than 3 month) + 0.103 (number of chicken ownership) + 0.741 (cage with soil floor) - 0.890 (drinking water of chicken originated from wells)Keywords: Campylobacter jejuni, logistic regression, village chicken, drinking water, wells

Expression of Bone Morphogenetic Protein-2 forMeasuring Effectiveness of Freeze Dried Bovine Bone Xenografts (FDBBX) Biomaterials as Bone ConnectorMaterial Agus Purnomo; Dhirgo Adji
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Osteogenic activity in normal fracture healing will start early at around 1 week after the repair , but not necessarily occur in fracture healing with the aid of grafts. Demineralized freeze dried bovine bone xenograft (DFDBBX) is a bone graft material made from cows that are widely produced recently to assist the process of bone grafting in humans, but has not been used on animals. It does not know how it works in helping to repair the bone. T wenty male SD rats weighing 150 g, were divided into 2 groups of 10 rats each. Group I was the control group, the rats were fractured and reconnected immediately using intramedullar pin fixation, Group II was a group of rats that were fractured and reconnected immediately using pin fixation intramedular plus graft material of freeze dried bovine bone xenograft. Fracture of the left femur was made in diaphysis part of bone. Evaluation of joints performed regularly at 1 week , 2 weeks, 4 weeks, 6 weeks and 8 weeks post surgery respectively by selecting at random, each group of 2 rats. Rats at each observation were collected two head / group, rats were then killed, the bone parts to be joined was taken and stored in 10% of formalin for histopathologic examination. Some pieces of the bones were used for the analysis of bone morphogenetic protein 2 (BMP-2 ) by immunohistochemical method. The results were then analyzed descriptively to see the changes directly on the X-rays and tissue. Overall results of this study showed that the graft material of DFDBBX were osteocondusive rather than osteoinductive properties. Nevertheless, the biomaterial is useful for orthopedic purposes primarily of handling cases with partial fracture of bone tissue which is lost. Due to the nature osteocondusive of DFDBBX will make cells to grow and to unify the separate bone fragments.Keywords : fracture, osteogenic activity , demineralized freeze dried bovine bone xenograft, bone morphogenetic protein-2, immunohistochemical analysis

Immunocytochemistry Streptavidin Biotin: Early Detection of Viral Nervous Necrosis Virus in the mucous of the Ikan Kerapu Macan(Epinephelus fuscoguttatus) Putu Eka Sudaryatma; Artanti Tri Lestari; Ni Luh Sunarsih; Ketut Sri Widiarti; Sulis NurHidayah; Didik Srinoto Srinoto
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Kerapu macan (Epinephelus fuscoguttatus) is one of the kerapu fishes that has been successfully bred by the farmers. The survival rate of the fish reaches up to the 40% at the fish hatchery of the home industry of in Bali. The disease in which may cause significant mortality in kerapu fish, especially for the larvae and juveniles is viral nervous necrosis (VNN). We therefore developed and applied the immunological diagnostic approach of immunocytochemistry technique of streptavidin biotin (SB) for early detection of VNN. Mucous samples of VNN infected- kerapu fish in vivo were firstly detected by reverse transcriptase-polymerase chain reaction (R T -PCR) for the presence of DNA VNN. If it is RT -PCR-positive VNN, the mucous samples were then tested by SB. Results of the present study indicated that the VNN virus could be detected with SB technique within only 24 hours post infection. It was concluded that rapid and accurate SB technique is suitable and appropriate to be applied for routine control and prevention national program in the Fish Quarantine for Indonesia because no need of fish sacrification, and scientific, law and international accepted, and even no hazardous environmental contamination.Keywords: kerapu macan (Epinephelus fuscoguttatus),VNN, mucous, SB, early detection

Herd Immunity Against Rabies Among Dogs in Ambon Astri D T agueha D Tagueha; Setyawan Budiharta; Heru Susetyo
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The objectives of this study were to reveals factors associated with level of herd immunity against rabies. Four hundred and eighteen blood samples were collected from five subdistricts and 14 villages using stratified and household cluster methods. The result in ELISA test is ≥ 0,5 IU/ml, dog titers sample was stated as protective to rabies. The result showed that the prevalence of protective antibody titers was very low i.e. 3.35%. Linear regression analysis reveals subdistrict of Nusaniwe, subdistrict of Baguala, dog owner education, post-vaccination period of 0 - 6 months, sex, and dogs kept as a guard house contribute to protective level of antibody titers. Unweighted logistic regression revealed that subdistrict of Baguala (OR = 0.05), subdistrict of Sirimau (OR = 0.09), dog kept as a guard house (OR = 3.96), dog owner education (OR = 12.29), and post-vaccination period of 0 - 6 months (OR = 27.08) are significantly associated with dog's immunity . V accination programs need to be improved by considering the four factors that emerged in these two regression results.Keywords : rabies, level of dog's immunity , ELISA, Ambon, post-vaccination

Examination of Viral Nervous Necrosis Virus in The Water Sample of The Tiger Grouper (Epinephelus fuscoguttatus) with Immunocytochemistry of Streptavidin Biotin Putu Eka Sudaryatma; Artanti Tri Lestari; Yenny Trisnasari; D Lia Lidayana; Wahyu Nurlita
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract One of potencies of marine waters that is developed, and starts to have the international market is grouper fish. Tiger grouper culture can not be separated from disease factors that may attack and destroy the cultivation. One of the important diseases that can cause mass mortality in fish, especially in grouper larvae and juveniles stadium is viral nervous necrosis (VNN). Examination by using immunocytochemical method of streptavidin biotin (SB) undertaken to develop early detection of VNN in order to be applied in preventing spread of the VNN disease in seeds of tiger groupers that are already distributed worldwide in Indonesia. And also, this SB methodology can also be applied to the tiger groupers without any injuring or even killing of the fish for the purpose of sampling. In the present study, the preliminary test was done to determine the infectivity level of the inoculum (VNN virus) owned by the Office of Fish Quarantine, Quality Control, and Fishery Safety Class I Denpasar, Bali. Whereas, the main test was done by collecting water sample from tiger grouper fish farming that was infected with VNN. The water sample was examined by using immunocytochemical method of SB after being confirmed by RT-PCR-positive test that was previously purified. Based on both SB and PCR tests having VNN positive results, it is concluded that the immunocytochemical method of SB could be applied as early, rapid and accurate detection of VNN in only a 24 hours post infection by using water as the sample. Keywords: VNN, water sample, streptavidin biotin, RT-PCR, tiger grouper

The Development and Application of Reverse Transcription Loop-Mediated Isothermal Amplification for The Diagnosis of Infectious Myonecrosis Virus in the White Shrimp (Litopenaeus vannamei) Zakiah Widowati; Firma Firma; Dita Rustian; M. Tony Wartono Silaban; Freddy Riatmo; Sulistia Trikora Astuti; Nurlaila Nurlaila; Asep Dadang Koswara; Hastari Wuryastuti
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Infectious myonecrosis virus (IMNV) is one of the shrimp viral diseases caused by a double-stranded RNA virus, non-enveloped, and belongs to the family group Totiviridae. Weakness diagnosis of RNA viral diseases due to the RNA viruses are unstable and highly reactive. So far the diagnostic methods for detecting IMNV with high accuracy is based on biomolecular test, such as reverse transcription polymerase chain reaction (RT-PCR). The RT PCR generally requires 4-8 hours of testing time, and also requires thermal cycler with a commercial kit materials which is relatively expensive. The purpose of the present study is to find and determine an alternative isolation of RNA from the samples and to determine whether the reverse transcription loop-mediated isothermal amplification (RT-LAMP) method based on biomolecular approach can be an alternative testing method for diagnosis of IMNV in the white shrimps(L.vannamei).The RT-LAMP is more simple, straightforward, but it has a high sensitivity. Direct and indirect RNA extraction methods from samples and FTA cards give good results as a template in RT-PCR and RT-LAMP IMNV. The FTA card as one of the RNA samples of storage media provides an alternative storage method that is easy, simple and has a shelf life of RNA in the long term (up to 6 months). Incubation time of RT-LAMP IMNV at 400C is between 15-30 minutes. Amplification time of RT-LAMP IMNV at 56o C is between 70-110 minutes. In the present study, the white shrimps (L.vannamei) that are positive IMNV-RT-LAMP provides 100% compliance results when tested by the RT-PCR method. The time of RT-LAMP amplification IMNV ranged from 70-110 minutes shorter than that of conventional PCR (180-240 minutes). Overall the RT-LAMP method for IMNV can be applied as a method of testing for confirmative IMNV diagnosis in the white shrimps (L.vannamei). Keywords: IMNV, RT-LAMP, RNA, FTA cards, white shrimp (L. Vannamei)

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