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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 857 Documents
 82   83   84   85   86 
Uji Kuantitatif PCR Gen CYP11A1 pada Sapi yang Mengalami Corpus Luteum Persisten Sri Gustari; Asmarani Kusumawati; Yonathan Alvin Maruli Asi Sihotang; Ruri Fitriyani; Fadlilah Tri Gholin Qurroh; Erif Maha Nugraha Setyawan; Agung Budiyanto; Aris Junaidi; Yosua Kristian Adi; Ghoyati Ulya Diniya; Aqilah Makmum
Jurnal Sain Veteriner Vol 44, No 2 (2026): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.113278

Abstract

Corpus luteum adalah kelenjar endokrin sementara yang terbentuk dari pecahnya folikel setelah ovulasi yang sangat penting untuk memelihara kebuntingan pada mamalia Corpus luteum persistent (CLP) adalah keadaan dimana corpus luteum tidak mengalami regresi dan tetap tinggal pada ovarium dalam waktu yang lama sehingga menjadi salah satu penyebab penurunan tingkat produktivitas sapi betina karena terdapat gangguan reproduksinya. Gejala klinis dari CLP yang sering terlihat adalah sapi mengalami anestrus dalam waktu yang lama sehingga proses reproduksi menjadi terhenti. Gen CYP11A1 diketahui memiliki peran dalam regulasi hormon prostaglandin yang berperan penting untuk regresi corpus luteum (CL). Penelitian ini bertujuan untuk melihat secara kuantitatif gen CYP11A1 pada sapi yang mengalami CLP. Analisis pita DNA dilakukan untuk melihat keberadaan gen tersebut pada sapi CLP. Hasil penelitian menunjukkan terdapat peningkatan ekspresi gen CYP11A1 pada sapi yang mengalami CLP dibandingkan normal. Hal ini diharapkan dapat memberikan informasi tentang potensi CYP11A1 sebagai marker untuk diagnosa sapi yang mengalami CLP sehingga dapat digunakan sebagai marker seleksi sapi betina produktif atau unggul.The corpus luteum is a temporary endocrine gland formed from the ruptured follicle after ovulation, which plays an essential role in maintaining pregnancy in mammals. Persistent corpus luteum (PCL) is a condition in which the corpus luteum fails to regress and remains in the ovary for a prolonged period, thereby becoming one of the causes of reduced productivity in female cattle due to reproductive disorders. The clinical symptom commonly observed in PCL is prolonged anestrus, causing the reproductive process to cease. The CYP11A1 gene is known to play a role in the regulation of prostaglandin hormones, which are important for corpus luteum (CL) regression. This study aimed to quantitatively evaluate the CYP11A1 gene in cattle affected by PCL. DNA band analysis was performed to determine the presence of this gene in PCL cattle. The results showed an increased expression of the CYP11A1 gene in cattle with PCL compared to normal cattle. These findings are expected to provide information regarding the potential of CYP11A1 as a marker for the diagnosis of cattle with PCL, so that it may be used as a selection marker for productive or superior female cattle.
ANTIBIOTIC RESISTANCE PROFILE OF STAPHYLOCOCCUS AUREUS ISOLATED FROM CLOACAL SWABS OF LAYER CHICKENS IN BANGLI REGENCY, BALI Yeocelin Meida Utami; Ni Wayan Helpina Widyasanti; Putu Henrywaesa Sudipa; I Nengah Kerta Besung; Grace Febiola Sinambela; Brigitta Milia Rosaline; Sasha Syaloom Stephannie Senduk
Jurnal Sain Veteriner Vol 44, No 2 (2026): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.114036

Abstract

Antibiotic resistance in livestock is an increasingly significant issue that affects animal health, food safety, and public health. Staphylococcus aureus is an important pathogenic bacterium in layer chickens and has the potential to serve as a source of resistant bacterial spread. This study aimed to determine the antibiotic resistance profile of S. aureus isolated from cloacal swabs of layer chickens in Bangli Regency, Bali. A total of 20 cloacal swab samples from clinically healthy layer chickens were cultured on Mannitol Salt Agar and identified through Gram staining, the catalase test, and mannitol fermentation characteristics. Antibiotic susceptibility testing was performed using the Kirby–Bauer disk diffusion method and interpreted according to Clinical and Laboratory Standards Institute. The results showed that 12 isolates (60%) were identified as S. aureus, with a higher prevalence in layer chickens (80%) compared with pre-layer chickens (40%). The highest resistance was observed against erythromycin (91.67%), followed by ciprofloxacin and sulfamethoxazole–trimethoprim (each 41.67%), while all isolates were sensitive to chloramphenicol. These findings indicate that S. aureus from layer chickens in Bangli Regency exhibits a high level of resistance to erythromycin, whereas chloramphenicol remains effective. The results highlight the need for prudent antibiotic use, strengthened biosecurity, and routine resistance monitoring to prevent the spread of resistant bacteria in layer farms.
Corneal Inflammation Induced by Bacillus thuringiensis: Implications for Bioinsecticide Safety Cicih Komariah
Jurnal Sain Veteriner Vol 44, No 2 (2026): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.116672

Abstract

Bacillus thuringiensis (Bt) is widely used as a microbial agent in bioinsecticide formulations and is generally regarded as safe for non-target organisms. However, clinical reports of corneal ulceration following ocular exposure to Bt-based products have raised concerns regarding its potential toxicity to the eye. This study aimed to evaluate corneal epithelial integrity, histopathological changes, neutrophil infiltration, and the expression of inflammatory and oxidative stress markers, including tumor necrosis factor-alpha (TNF-α) and malondialdehyde (MDA), in Wistar rats following topical exposure to Bt bioinsecticide. A post-test-only control group design was applied using 24 male Wistar rats divided into one control and three treatment groups receiving Bt concentrations of 8 g/L, 10 g/L, and 12 g/L. Corneal epithelial defects were assessed using fluorescein staining, while histological examination and semi-quantitative measurements of neutrophils were performed on enucleated eyes. TNF-α levels were measured using ELISA, and MDA levels were determined via the thiobarbituric acid (TBA) assay. There was epithelial desquamation, increased epithelial thickness, and higher neutrophil infiltration in the treatment groups compared to controls, although these parameters did not reach statistical significance. Notably, TNF-α levels demonstrated a significant increase in the 10 g/L treatment group (p = 0.023), indicating activation of local inflammatory pathways. MDA levels showed a non-significant upward trend. Topical exposure to Bt can elicit corneal inflammatory responses even in the absence of clear structural damage, highlighting the need for caution regarding ocular contact with Bt-based bioinsecticides
ISOLASI DAN IDENTIFIKASI BAKTERI Escherichia coli PATOGEN PADA BURUNG KUNTUL BESAR (Egretta alba) DI KECAMATAN BAITUSSALAM Dilla Suhelmi Putri; Masda Admi Admi; M Daud AK; Erina Erina Erina; Riyan Ferdian Ferdian
Jurnal Sain Veteriner Vol 44, No 2 (2026): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.116783

Abstract

Burung Kuntul Besar (Egretta alba) adalah burung air yang umum ditemukan di daerah pesisir dan ekosistem hutan bakau serta memiliki interaksi yang erat dengan lingkungan perairan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi patogen Escherichia coli pada Burung Kuntul Besar (Egretta alba). Penelitian ini dilakukan dengan menggunakan desain observasi lapangan dengan pendekatan potong lintang, dan isolasi bakteri dilakukan berdasarkan metode Carter. Hasil penelitian menunjukkan pertumbuhan bakteri pada media Nutrient Broth (NB), yang ditunjukkan oleh perubahan media dari jernih menjadi keruh. Isolasi pada Eosin Methylene Blue Agar (EMBA) menunjukkan koloni berwarna hijau metalik, yang merupakan ciri khas E. coli. Pewarnaan Gram menunjukkan bahwa bakteri yang diisolasi bersifat Gram negatif. Identifikasi lebih lanjut menggunakan Sorbitol MacConkey Agar (SMAC) menunjukkan adanya koloni tak berwarna , yang mengindikasikan E. coli patogen. Dapat disimpulkan bahwa E. coli patogen, sebagaimana dikonfirmasi oleh uji SMAC, terdeteksi pada 10 Burung Kuntul Besar (Egretta alba) yang diami di kawasan hutan bakau Baitussalam, Aceh Besar. 
Prevalensi dan Karakterisasi Ektoparasit Mallophaga pada Burung Sriti (Collocalia linchi) di Kluet Tengah, Aceh Selatan M Hanafiah
Jurnal Sain Veteriner Vol 44, No 2 (2026): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.117717

Abstract

Burung sriti (Collocalia linchi) merupakan famili Apodidae dan tersebar luas di kawasan Asia Tenggara. Burung sriti juga berpotensi menjadi inang ektoparasit Mallophaga yang dapat memengaruhi kesehatannya. Penelitian ini bertujuan untuk mengetahui prevalensi dan karakterisasi ektoparasit Mallophaga pada burung sriti (Collocalia linchi) di Kecamatan Kluet Tengah, Kabupaten Aceh Selatan. Sebanyak 10 ekor burung sriti diperiksa menggunakan metode penyikatan tubuh untuk mengoleksi ektoparasit. Hasil penelitian menunjukkan bahwa 7 ekor burung sriti (70%) terinfeksi ektoparasit Mallophaga. Total kutu yang ditemukan sebanyak 15 ekor yang terdiri dari dua genus, yaitu Menacanthus sp. terdapat sembilan kutu dan Goniocotes sp. terdapat enam kutu. Hasil ini menunjukkan adanya infestasi ektoparasit Mallophaga pada burung sriti di lokasi penelitian.  
Nematode Infection Level in Capra hircus on Oesao Rice Fields Area, Kupang Regency, Indonesia I Gusti Komang Oka Wirawan; Andrijanto Hauferson Angi; Yanse Yane Rumlaklak Rumlaklak; Ni Wayan Apsari Shantika Pratistha; Suryawati Suryawati Suryawati; Stormy Vertygo
Jurnal Sain Veteriner Vol 44, No 2 (2026): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.118608

Abstract

Research objective: to determine the diversity and infection levels of gastrointestinal nematodes in Capra hircus based on age differences in Desa Oesao, Kabupaten Kupang. A total of 30 fecal samples were collected, consisting of 15 samples from young goats and 15 from adult goats, without sex differentiation. Grazing field temperature was also recorded. Samples were examined using sedimentation and flotation methods to identify helminth eggs, while infection intensity was determined using the McMaster method and expressed as eggs per gram (EPG) of feces. The results showed similar nematode diversity in both age groups, except for Chabertia ovina and Ostertagia sp., which were not detected in adult goats. T-test analysis indicated that infection levels in young goats were significantly higher (p < 0.05) than in adults. In young goats, infection levels were highest for Strongyloides papillosus (1,318 EPG) and Haemonchus contortus (700 EPG), followed by Chabertia ovina (67 EPG), Trichostrongylus sp. (63 EPG), Oesophagostomum sp. (43 EPG), Trichuris sp. (43 EPG), and Ostertagia sp. (20 EPG). In adult goats, lower EPG values were recorded for the same species except Chabertia ovina and Ostertagia sp. Infection diversity and intensity were influenced by immunity, age, feed sources, and grazing environment
Deteksi Bakteri Citrobacter freundii sebagai Indikator Higiene dan Sanitasi pada Proses Produksi Ikan Asap di Kota Kendari: Kajian Epidemiologi Pangan Lymbran Tina; Herwin Pisestyani; Sufiah Asri Mulyawati
Jurnal Sain Veteriner Vol 44, No 2 (2026): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.119109

Abstract

Keamanan pangan pada produk ikan asap tradisional masih menjadi perhatian dalam kesehatan masyarakat, terutama terkait dengan praktik higiene dan sanitasi yang belum optimal. Penelitian ini bertujuan untuk mendeteksi keberadaan Citrobacter freundii sebagai indikator higiene dan sanitasi, serta mengkaji implikasinya dalam perspektif epidemiologi pangan pada proses produksi ikan asap di Kota Kendari. Penelitian ini menggunakan desain observasional dengan pengambilan sampel pada 13 unit pengasapan. Sampel ikan diambil pada dua tahap, yaitu sebelum dan sesudah pengasapan, dengan dua kali pengulangan pada masing-masing tahap, sehingga diperoleh total 52 sampel. Selain itu, dilakukan observasi terhadap kondisi higiene dan sanitasi unit pengasapan. Hasil penelitian menunjukkan bahwa C. freundii terdeteksi pada 61,5% sampel sebelum pengasapan dan 50% sampel setelah pengasapan. Penurunan ini menunjukkan bahwa proses pengasapan memiliki efek reduksi, namun belum mampu mengeliminasi cemaran mikrobiologis secara optimal. Hasil observasi menunjukkan bahwa sebagian besar unit pengasapan belum menerapkan praktik higiene dan sanitasi yang memadai, terutama pada aspek kebersihan pekerja, peralatan, dan fasilitas sanitasi. Secara epidemiologis, keberadaan C. freundii pada produk akhir mengindikasikan adanya potensi kontaminasi silang dan risiko paparan mikroorganisme melalui konsumsi ikan asap. Oleh karena itu, peningkatan praktik higiene dan sanitasi pada seluruh tahapan produksi diperlukan, serta penggunaan C. freundii dapat dipertimbangkan sebagai indikator alternatif dalam evaluasi keamanan pangan pada pengolahan ikan asap tradisional.Kata kunci: Citrobacter freundii, higiene dan sanitasi, ikan asap, epidemiologi pangan

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