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Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 824 Documents
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DETECTION PROTECTIVE ANTIGEN WITH MONOCLONAL ANTIBODY OF PASTEURELLA MULTOCIDA DOMPU ISOLATE Sri Murwani; Widya Asmara
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8599

Abstract

Monoclonal antibodies can be used as specific probes for molecular structure such as drugs, hormones, receptor for hormones, or any other biologycally derived or biology-cally active materials. The potential usefulness of mono­clonal antibodies is in almost every field of biology and medicine.The study was carried out to produced monoclonal anti­bodies against outer membrane protein (OMP) Pasteurella multocida isolat Dompu, which can be used to isolate imunogenic OMP fractions.Three monoclonal antibodies which specifically react with OMP fraction of Pasteurella multocida isolat Dompu were obtained previously. Those monoclonal antibodies were pro­duced in Balb/c mouse. The antibodies production were measured by ELISA and were purified by affinity chromatograPhy.The monoclonal antibody were injected intraperitoneally into group of five rats. Two additional groups of five rats were injected with immune mouse serum and normal mouse serum, which served as a positive and negative controls respectively. All rats were challenged with 1 ml lethal dose of Pasteurella multocida 24 hours after the antibody injec­tion.The outer membrane protein was separated 12,5% SDS-PAGE. The region of gel containing the appropiate antigens (PI, P2, P3) were excised. Protein antigen was mixed with incomplete Freund's adjuvant (IFA) and injected intramuscu-lary into group of five rats at dose 0,2 ml per mouse. Two additional groups were injected with gel in IFA and OMP and IFA as a positive and negative controls. Each treated mouse and control was immunized four times, at weekly interval. Two weeks after final immunization rat were bled and their antibodies productions were measured with ELISA. All mice were then challenged with lethal dose of Pasteurella multo­cida.The result indicated that eventhough immungenic the PI, P2, P3 OMP fraction individually were not able to induce protective antibody. 
IDENTIFICATION OF SERPTICAEMIA EPIZOOTICA (SE) ON SWAMP BUFFALOES (KALANG BUFALLOES) IN SOUTH KALIMANTAN Bambang Nganji Utomo; Tarmudji Tarmudji; A. Hamdan
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8601

Abstract

Identification of Septicaemia Epizootica (SE) was carried out on swamp buffaloes at the districts of Hulu Sungai Tengah (HST) and Hulu Sungai Utara (HSU) and those which were slaughtered in the abbatoars of Banjarmasin, Banjarbaru and Amuntai, South Kalimantan. This study was conducted to find out whether Septicaemia Epizootica was present among the swamp buffaloes population. Thirty two samples of swab nasopharynx from the field (HSU and HST) and 60 samples from the abbatoars were collected and checked toward Pasteurella multocida. And 45 samples of sera were examined for antibody with Elisa method. Based on bacterio­logical examination there was no Pasteurella multocida could be identified from the samples and from Elisa examination was also negative from Septicaemia Epizootica.
ISOLATION, IDENTIFICATION AND SENSITIVITY TEST OF BACTERIA FROM DIARRHEA CASES IN DOG AT THE VETERINARY CLINIC, FACULTY OF VETERINARY MEDICINE, GADJAH MADA UNIVERSITY Yuriadi Yuriadi; Ida Tjahajati
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8602

Abstract

A study was conducted to find out bacteria which probably play an important role in causing diarrhea in dog at the veterinary clinic Gadjah Mada University. Sample were collected from diarrhea cases of dog, during the periode of September to December 1997, by rectal swabbing. Samples were then sent to the laboratory for bacterial isolation and identification.The result indicated that E. coli was found from 50% of the sample, Streptococcus sp 41.9%, Staphylococcus sp 33.3% of sample, Enterobacter sp 16.7%, Bacillus sp 8.3%.Some of E. coli isolates were pathogenic to mice. The pathogenic E. coli were resistant to penicillin, ampicillin, streptomycin, erythromycin, doxycycline, besides sensitive to neomycin, gentamycin, kanamycin, polymyxin B, chloram-phenicol and sulfisoksale.
The Susceptibility Of Burkholderia Pseudomallei Strains To Various Chemotherapeutics : a review Jeanne Indriana
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8604

Abstract

...
EFFECTS OF ACTIVE GUMBORO VACCINE OF INTERMEDIATE STRAIN HIGH PATHOGENICITY ON THE MORPHOLOGY OF BURSA FABRICIUS AND THE IMMUNE RESPONSE OF BROILER TO VACCINATION AGAINST NEWCASTLE DISEASE Charles Rangga Tabbu
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8606

Abstract

This experiment was designed to study the effects of active Gumboro vaccine of high pathogenicity on the morphology of bursa Fabricius and the immune response of broiler to vaccination against Newcastle disease (ND). This experiment was conducted in 330 broilers, which were vaccinated with aviive Gumboro vaccine of high, intermediate and low pathogenicity (control) at the age of 12 days. Experimental chickens were also vaccinated against ND at the age of 4 and 18 days. Diameter of bursas were measured at day 1st through 7th, 14th, 21th, 28th, 35th post Gumboro vaccination. Evaluation of hemaggutination inhibition (HI) liters against ND virus (NDV) were done only at day 7th, 14th, 2lth, 28th and 35th. Samples of bursa were stained with the method of hematoxylin and eosin (H & E). Pathologic evaluation revealed lesions in the bursa Fabricius of chickens vaccinated with Gumboro vaccine of high pathogenicity as well as changes in the diameter of bursa of lesions in this organ. The HI titers against NDV were lower in the group of chickens vaccinated with Gumboro vaccine of high pathogenicity compared with them of other groups. Results indicated that active Gumboro vaccine of high pathogenicity caused lesions in the bursa,which were similar to lesions caused by exposure to field Gumboro virus.
EFFECTS OF SILVER INDUSTRY WASTE WATER IN KOTAGEDE, YOGYAKARTA ON HAEMTOLOGlCAL AND HISTOPATHOLOGICAL CHANGES IN EXPERIMENTAL RATS (RATTUS NORVEGICUS) Bambang Hariono
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8607

Abstract

Thirty male rats, 2,5 months old with average body weight of 92 grams were used as experimental animals.The experiment rats were adapted before for one week. The animal grouping was basedon uniformity of body weight gain, Thecontrol group consist of 15 animals and 15 animals for treated group. The treated group was given silver industry waste water from Kotagede, Yogyakana orally ad libitum the control group was supplied drinking tap water ad libitum. Blood samples via plexus retroorhitalis vein at medial cantus of the eye were taken for routine heamiolafiical examinations, clinical symptoms and body weight gain were also recorded. After treatment, for about 12 weeks, all experimental animals were sacrificed, necropsied, then the samples of brain, spinal cord, lung, tesiis. liver, and kidney were taken for histopathological preparations. The concentration of metals (especially Ag and Cu) in the liver, silver industry waste water, well water, Tap water, and soil samples (which [he silver industry waste water was discarded) were analyzed. The results of the experiment indicated thai the silver industry waste water administration in long period caused decreasing of the rat growth rate with clinical symptoms e.g. thinness,dullnessof the fur, anaemic, depression and nerve symptoms including foot paralysis. Statistical calculation showed there were not significantly changes on hematological profile between the control and treated groups, it could be due to severe dehydratation level occured, so there were reducing of blood volume. This finding was similar to leucocyte and differential counts, but there tended to decrease at week-12. There could be due 10 bone marrow depression which were caused by heavy metal intoxication. Microscopical examinaton showed there were thickness of alveoli septa, congestion, enlargement of sinusoids and infiltration of mononuclear cells around the liver centralvein, degeneration and necrosis of tubule cells, enlargement of tubule lumen and Bowman capsule space, infiltration of monoclear cells and haemorrhage performance. There were a few Purkinje cells showed more denses and the sTain more absorpted intensively (the same findings occuredin the nerve cells of spinal cord), it could be possible that there were cell structural changes, degeneration and necrosis of The nerve cells especially in the spinal cord tissues. The silver, copper and chrome concentrations in the silver industry waste water and soils samples were too high, it could be as potential sources for environmental pollution. The concentration of these metals in the tap water and well water were not detected yet.
IMMUNOGENICITY AND PROTECTIVITY OF THE OUTER MEMBRANE PROTEIN BANDS OF PASTEURELLA MULTOCIDA ISOLATE U-6 Ignatius Mulyadi; Widya Asmara; B. Sardjono
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8608

Abstract

The most immunogenic and protective fraction of outer membrane proteins of Pasieurella multacida has not well understood. This study was done to find our the immunogenicity and protectivity of outer membrane protein fractions of Pasteurella multicida isolat U-6 in mice. The organism was cultured in Luria Bertani broth. The outer membrane proteins were prepared according 10 Snipes et al., (1988), Electrophoresis of the protein was carried out in 10% of sodium dodecy 1-su I ph ale poly cry 1 amide gel electrophoresis, and stained with 0.3% Coomasie brilliant blue. The result indicated that there arc many protein bands with 5 major protein bands, those are having molecular weight of 20,1 kilo Dalton (kD), 27 kD, 36 kD, 52,7kD,and75 kD. Each protein bandof molecular weight of 27 kD, 36kD,and75kD was then cut, and homogenised, and was used as antigen to immunise the mice after added with adjuvant 1:1. Two weeks after immunisation all of the mice were bled and the sera were tested by ELISA. FourtyeigM hours from bleeding, ail of the immunised and control mice were challenged with 500 colony forming unit of virulent Pasteurefla mutticida isolate U-6, and observed their mortality. The result indicated thai the outer membrane protein bands of molecular weight of 36 kD and 75 kD were immunogenic (P>0,05), while the outer membrane protein band of 27 kD was less immunogenic (P>0.05), Band with moleculer weight of 27 kD produced 0% protection, bandof 36 kD produced 60% protection, and band of 75 kD produced 40% protection of mice challenged.
INTERPRETATION OF DIAGNOSTIC TESTS IN CLINICAL EPIDEMIOLOGY Bambang Sumiarto
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8609

Abstract

A test is process designed to detect a sign, substance tissue change, or response. The detection of signs or tissue abnormalities during [he diagnostic process, pregnancy diagnostic activities, and meat inspection findings, are also tests. Indeed, tests are used in virtually all problem solving activities and therefore the understanding of the principles of lest evaluation and interpretation are basic to many of practical veterinarian and researcher. Clinical epidemiology revolves around the use and interpretation of tests. 
ADHESION AND PHAGOCYTOSIS PROPERTIES OFSTREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS ISOLATES FROM INDONESIA Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8610

Abstract

An infectious diseases outbreak affecting pigs and monkeys in Bali in 1994, with case fatality rate (CFR) 75%. The disease widespread rapidly to the other district in Indonesia and developed serious problems. The causative agent involving the disease was Streptococcus equi subsp. zooepidemicus, a β-hemoIytic type of the streptococcus. The disease was consistently associated with meningitis, therefore the disease was named Streprococcal meningitis. The main aim of this study is todetect factors promoting the pathogenicity of the agent that could be showed by its characteristics and properties of the adhesions and phagocytosis. Eight and two isolates of Strepiacoccus equi subsp. zooepidemicus originated, from pigs and monkeys respectively were used in this study. The nature of bacterial surface proteins was characterized by hexadecane adherence test, hemaglutinating erythrocytes of various animals, adherence to epithelial cells and phagocytosis of the bacteria by polymorphonuclear leucocytes. The result of hexadecane adherence test showed that of the ten isolates oniy one culture of pigs isolates had a hydrophobic surface and the others were hedrophilic surface. The culture with hydrophobic surface agglutinated erythrocytes from chicken, rat and rabbit. This culture adhered in high numbers to epithelial cells and were more phagocytosed by polymorphonulear leucocytes. The cultures with hy drop hi lie surface showed no comparable reaction.
MONOCLONAL ANTIBODIES AGAINST OUTER MEMBRANE PROTEIN PASTEURELLA MULTOCIDA STRAIN DOMPU Sri Murwani; Widya Asmara
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8618

Abstract

Monoclonal antibodies were produced against outer membrane protein of Pasteurella multocida strain Dompu. The bacterial isolate was obtained from [he bacteria! collection held at Microbiology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Irs purity and patogenicily has beeen tested.Killed PatTeurella multocida were used ro immunized 7 female, 8-10 week old of Balb/ c mice. Cell fusion between limphocytes and myeloma cells (2:1) were carried out by PEG stimulation and bybrids were cultured in  HAT medium.The OMP were prepared from sonicated organism using centrifugallon in the presense of sarcosyL These antigens were than used to select antibody producing hvbridomas ELISA and cloning of hybridomas were done by serial dilution method.Four hybridomas clones were obtained, till producing monoclonal antibodies which reacted specifically against OMP Pasietirelta multacida stain Dompu.

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