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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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FIELD EVALUATION OF NEWCASTLE DISEASE VACCINE OF VG\GA IN STRAIN SEVERAL COMMERCIAL BROILER FARMS IN JAVA Charles Rangga Tabbu
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8643

This field evaluation was conducted to study the level of protection, post vaccinal reactions and zootechninal performance of groups of chickens which have been vaccinated with Newcastle disease (ND) vaccine of VG/GA strain.This evaluation was done in 26.000 broilers from 4 farms, which have different condition in management practices and were located in different areas of Java.Chickens were vaccinated with VGAGA vaccine or with B-l/La Sota vaccine with/without inactivated ND. Serological tests were done at the age of 28 and 35 days, whereas challenge test with local ND virus isolates was done at the age of 45 days. Results of field evaluation indicated that in broilers, the VG/GA vaccine induced a high level of protection agamst the challenge test with a local (Indonesia) ND virus isolate and against infection with field ND virus. Statistical analysis indicated that the hemagglutination inhibition (HI) titers against NDV were significantly higher (P < 0,05) in group of chickens vaccinated with VG/GA with/without inactivated ND compared to group of chickens vaccinated with B-l/La Sota with/without inactivated ND vaccine. In certain conditions, the post vaccinal reactions were milder and the zootechnkal performance was better in groups of chickens vaccinated with VG/GA strain compared to groups vaccinated with B-l/La Sota.

ARTIFICIAL INFECTION STUDY OF FASCIOLA GIGANTICA IN LOCAL SHEEP AND ITS IMPACT ON WEIGHT GAIN, HEMATOLOGY AND ENZYME GAMMA GLUTAMYL TRANSFERASE Khajadatun Khajadatun; Wardiarto DP; Setyawan Budiharta
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8644

This research was to investigate the effect of infection of Fasciola gigantica on weight gain, blood picture and the level of iiver damage of local sheep, and the correlation between the number of metacercariae infectH and the number of the fluke recovered. Twenty male local sheep, were allocated randomly into 4 different groups. One group used as uninfected control and a total of 200, 500 and 1.000 metacercariae was infected into animals of groups 2, 3 and 4, respectively. Variables evaluated include weight gain, blood picture, gamma glutamyl tons/erase (GGTase) activity, and the number of egg and the fluke recovered. All individual sheep were weighed every week, whole blood and sera were taken every 3 weeks, fecal samples were started to b« examined 8 weeks after infection and every week thereafter. Weight gain, hematology picture and enzyme GGTase activity were anafized by split-plot, the number of egg and fluke recovered were analized by correlation. All sheep were killed 18 weeks after infection. The results show that infection F. gigantica causes norntocytic normochromic anemia in local sheep. Increased GGTase acitivity is observed. The number of fluke recovered correlates with the number of metacercariae infected.

CROSS-SECTIONAL STUDY ON CAPRINE SARCOPTIC SCABIES IN THE DISTRIC OF Kl LONPROGO Samkhan Samkhan; Setyawan Budiharta; Wardiarto Darjoprawito; Bambang Sumiarto
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8645

A cross-sectional study of scabies was performed on 2615 goats from 492 small holder farmers, covered aC subdistricts and 40 out of 88 vfllages in Kulanprogo district, Yogyakarta Province. The Objectives of this study is to investigate (a) factors associated with the prevalence of the disease in goat, and (b) the prevalence uf thedisease among goat population in the district A combination of a three stage and duster sampling procedures was used to select the goat. An animal was considered as suffering from scabies if it showed cuaical sign of scabies and positive laboratory finding of Sarcoptes scabieLThe mportant findings of this study include significant association between sarcoptic scabies in the farm and cleanliness of the barn, animal density, new mcoming animal, new annual from outside of the village, intensive barn, separation of younger animal, between barn distance and closed barn. Other factos, Le. altitude, former's formal education and experience of goat raising are not significantly associated with the disease. In the animal level, new incoming animal, clinical signs, previous infestation and age are found to be associated with the occurrence of the disease. No significant association is found between the occurrence of sarcoptic scabies and sex and breed. The prevalence of the disease is found o be 93% (467492) and 73% (205/2615) for farm and animal level, i respectively.

CALCULATION OF % PROGESTERONE IN SKIM MILK FRACTION WHEN CENTRIFUGATION TEMPERATURE AND % BUTTERFAT OF WHOLE MILK ARE KNOWN Sri Hartantyo
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Air susu dikumpelkan dari 70 fkor sapi perah, Departemen Animal Science, Michigan State University, USA-, sampel-sampel tersebut diperlakukan terhadop berbagai temperatur perlakuan, yaitu : 0.10 C : I 0C : 30 C : 50 C : 100 C : 200 C : 300 C dan kontrol Periode inkubasi dilakukan 10 jam. Hasil dari studi ini menunjukkan bahwa dari grafik kalkulasi persentase progesteron pada skim milk apabila temperatur sentrifugasi dan kadar lemak susu diketahui maka diperoleh hasil bahwa semakin tinggi kadar lemak ternyata persentase progesteron dalam skim milk akan menurun. Dapat diketahui pula bahwa semakin tinggi temperatur, kadar progesteron dalam skim milk akain menurun sejajar dengan peningkatan kadar lemak.

EFFECTS OF RIBOFLAV1N SUPPLEMENTATION ON THE TERATOGENIC EFFECTS OF SODIUM BORATE IN WHITE RATS Tri Wahyu Pangestiningsih; Sumiyati Sunaryo
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Na-borate gave teratogenic effects both to rats and chickens. In chickens the fetal malformations caused by borate treatments were similar to those due to riboflavin deficiency. It was proposed that in embryonated eggs borate built a bond with riboflavin, so that riboflavin could not junction properly. This present investigation was conducted to assess the possibility of riboflavin in prevent the teratogenic effects of Na-borate in rats.Nineteen primigravida Wistar rats were allocated randomly to one of treatment groups, i. e, 9 rats were orally treated with 180 mg/200g BW/day, No-borate the other 10 rats beside treated with Na-borate, they were also treated orally with 2.5 mg/rat/day riboflavin. The treatments were done during the pregnancy on day 6 to 15. On day 20 the pregnant does were caesarean sectioned under ether anaesthesia. After all fetal malformations were noted, then all fetuses were placed in alcohol 70%, prepared for alizarin method for microscopic skeleton inspection. Chi square analysis of the gross inspection malformations, i e. the resorbed vs. the unresorbed, the alive vs. the dead, the haemorrhage vs. the non Hemorrhage fetuses of the two treatments showed unsignificant difference. Riboflavin supplementation increased singnificantly the number of fetuses with omphalocel. T-test analysis indicated that riboflavinsupplementation highly significantly caused the fore and hind paw, costal, sternal defects became more serious, if also significantly reduced the weight and the length of the fetuses. This investigation revealed that riboflavin supplementation failed to prevent, but even caused the teratogenic effects of the Na-borate to rats became more serious.

AN EFFORT IN CONTROLLING MYCOPLASMOSIS IN BROILER USING THE THIRD GENERATION OF QUINOLONE ANTIBIOTIC IN FIELD CONDITION Darjono Darjono
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

A total of 2251 broilers treated with 20 mg/kg body weight enrofloxacin and 1947 broilers treated with 20 mg/kg body weight ofloxacin plus 2260 broilers treated whith 125 mg/kg body weight chlortetracyclin were allocated in an experiment lo evaluate the effectiveness of the third generation quinolone in controlling mykoplasmosis in field.Result of this experiment demonstrated that These group nf antibiotics could produced lower mortality altogether with the culling rate and higher market weight compared to those treated with chlortetracyclin (P < 0.0 1). Although, treatment of mycoplasmosis in field using antibiotics in this group did not completely eliminate the clinical signs and patological changes as compared to the experiments done in the laboratory. Economical evaluation indicated that usage of These groups of antibiotics for broilers was more beneficial than chlortetracyclin.

RELATIONSHIP BETWEEN SURFACE HYDROPHOBICiTY, HAEMAGGLUTTNATION AND ADHERENCE MECHANISM OF STREPTOCOCCUS SUIS Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Streptococcus suis is well known as the causative agent of disease in various animal including pigs and human. The streptococcal cultures isolated from pigs and human have been characterized and identified biochemically and serologically as Streptococcus suis. The Cultures could be serotyped with 28 different monospecific antisera. Tne result of tins study indicated that the predominant antigen types of the S. suis cultures belonged to serotypes 2, 1/2, 1. The occurrence of the type specific microcapsule appeared to be realed with the surface hydrophobicity of the bacteria. Toe surface hydrophobicity could be demonstrated by hexadecan adherence tests. The S. suis cultures with hydrophobic surface agglutinated erythrocytes of humans, pigs and rabbits. The relationship between surface hydrophobicity, haemagglutinating and adherence properties of cultures could be demonstrated with 3 cell models. The haemagglutinating cultures adhered in high number to HeLa cells, ephitelial cells and to alveolar macrophages from rabbits. The non haemagglutinating cultures with hydrophobic surfaces adhered only to ephitelial cells and to alveolar macrophages S, suis cultures with hydrophilic surfaces showed no comparable reaction with 3 cell models.

THE USE OF DIFFERENT STAINING METHODS TO EVALUATE THE DEVELOPMENT AND VIABILITY OF BOVINE EMBRYOS OBTAINED IN VIVO AND IN VITRO Sri Gustari; Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In an attempt to find a good method to evaluate embryos we classified the in vitro embryos into freezable and non-freezable by subjective method. These embryos were compared with in vivo freezable embryos using Giemsa staining. The embryo development and viability was evaluated by fluorescent stain (PDA and Hoechst 33342) and compared with the result after Giemsa stain.That number of cells in freezable in vitro and in vivo embryos were not different (P > 0.1), while in vitro fresszable and non-freezable embryos showed significantly differences (P < 0,001) in number of cells.The agreement between Hoechst 33342 and Giemsa stain was quite high (63%) in embryos with number of cells less than 20, and low agreement (25 - 34 %) in embryios with number of cells more than 20. There was a significantly correlation (0.82) betwen the degree of fluorescent of FDA stain and the number of cells at Giemsa stain.The subjective classification of in vitro embryos can be used in the practical situation. PDA staining is a good tool to check this classification as well as to evaluate the development and viability of embroys in different step of experimental trials. Hoechst can be a valuable

Ancaman terhadap Masuknya Virus Penyakit Mulut dan Kuku melalui Daging Ilegal di Perbatasan Darat Indonesia-Malaysia Risma Juniarti Silitonga; Retno Damayanti Soejoedono; Hadri Latif; Etih Sudarnika
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan untuk menganalisis keberadaan daging ilegal di perbatasan darat Indonesia-Malaysia sebagai ancaman risiko masuknya virus PMK ke wilayah Indonesia. Data primer diambil menggunakan teknik pengumpulan pendapat pakar dengan kuisioner, wawancara mendalam (in-depth interview) dan pengamatan langsung di lapang. Data sekunder diperoleh dari publikasi ilmiah dan tulisan atau data yang tidak dipublikasi (statistik, dokumen dan laporan dari instansi berwenang). Penentuan responden secara purposive sampling. Hasil studi menunjukkan bahwa daging ilegal diperkirakan berasal dari berbagai negara termasuk dari negara/zona yang berstatus endemis PMK seperti Semenanjung Malaysia, Thailand, India dan negara/zona yang dinyatakan tidak diketahui oleh responden. Jenis daging ilegal yang masuk ke Entikong berisiko sebagai sumber infeksi PMK seperti daging beku bertulang tanpa limfoglandula dan jeroan beku tanpa limfoglandula. Berdasarkan jalur dan frekuensi pengangkutan, perkiraan volume pemasukan daging ilegal menunjukkan kemungkinan daging masih bisa lolos melalui jalur non-kendaraan. Kondisi-kondisi tersebut mengindikasikan bahwa pemasukan daging ilegal dapat sebagai ancaman risiko masuknya virus PMK ke Indonesia khususnya di perbatasan darat Indonesia-Malaysia, Entikong. Perlu dilakukan upaya pencegahan untuk mengurangi ancaman risiko yaitu dengan melakukan pengawasan yang lebih ketat di pintu-pintu pemasukan dengan koordinasi lintas instansi di perbatasan untuk bersama-sama mencegah pemasukan daging ilegal.

PENGARUH PEMBATASAN PAKAN DAN PEMENUHAN KEMBALI PAKAN TERHADAP KONSUMSI NUTRIEN KECERNAAN NUTRIEN, STATUS FISIOLOGI DAN PERUBAHAN BERAT BADAN KAMBING BLIGON Bambang Suwignyo; Ulil Amri Wijaya; Rieska Indriani; Asih Kurniawati; Irkham Widiyono; Sarmin Sarmin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.11603

Penelitian ini bertujuan untuk mengetahui kecernaan nutrien pakan dan status fisiologi pada kambing Bligon yang diberi perlakuan pembatasan pakan (feed restriction). Enam ekor kambing Bligon jantan umur rata-rata 12 bulan dengan bobot badan rata-rata 23,6 kg diberi pakan yang terdiri dari hijauanjerami kacang tanah (rendeng)dan konsentrat. Kambing dibagi menjadi 2 kelompok perlakuan. Tiga ekor kambing untukperlakuan kontrol diberi pakan berdasarkan kebutuhan bahan kering (BK) 3,5% dari bobot badan, dan tiga ekor kambing untuk perlakuan pembatasan pakandilakukan pembatasan pakan dengan pengurangan pakan 50% dari kebutuhan bedasarkan BK selama 35 hari. Variabel yang diamati adalah konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), perubahan berat badan, kecernaan bahan kering (BK), kecernaan bahan organik (BO), temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung. Data yang diperoleh dianalisis dengan independent samplesT-Test. Hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa pembatasan pakan (feed restriction) berpengaruh nyata terhadap penurunan konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), kecernaan bahan kering (BK) dan bahan organik (BO), tetapi tidak berpengaruh nyata pada temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung (pulsus) kambing Bligon. Pemenuhan kembali (refeeding) pakan berpengaruh nyata (P<0,05) menaikan kembali konsumsi BK dan konsumsi BO. Perubahan berat badan kambing mengikuti pola jumlah pemberian pakan. Saat pembatasan pakan, berat badan kambing perlakuan turun 55,24 g/hari dan ketika pakan dipenuhi kembali berat badannya meningkat 131,43 g/hari.

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