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INDONESIA
Jurnal Sain Veteriner
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 824 Documents
THE CHANGES OF THE TESTOSTERONE HORMONE LEVEL DURING PREPUBERTAL PERIODE IN INDUCED HYPOTHYROIDISM RATS Teguh Budipitojo; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8619

Abstract

The one the influence factors to the normal functions and testes growth is thyroid gland status. The knowledge about the influences of euthyroid, and hypenhyroid to thetesies growth will help to give more understanding of the normal lestes growth. The new born male rats were used todetect the influence of hypothyroidism to the testosterone level during prepubertal periode. Twenty primigravida pregnant rats (Rattus norvegicus albinus) were used. After birth, the male pups were divided inio controls and treatments group. Mothers of the treatment group were given 0,1% 6-n-prapyl-2-thiauracil (PTU), via drinking water, ad libitum, after birth until on day 8. On dary 27, 30, 37, 44, and 51, five control pups and 5 treatment pups were sacrificed. The blood from each pup were collected to detec serum testosteron hormone level using immunoradLometric assay (IRMA) method. The research showed that serum testosteron level ofthe; treated group increased on day 30 (P<0,05) and day 37(P<0,05). Theresultof This research showed that PTU induced hypothyroidism produced increasing serum testosterone level.
HISTOPATHOLOGICAL CHANGES OF SCHISTOSOMIASIS FROM NAPU VALLEY, CENTRAL SULAWESI, 1NDONESIA Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8620

Abstract

Schistosomiasis is an endemic disease caused by the infection Schistosoma Japonicum (Trematoda), can attack human and animals surrounding Lindu lake and Napu valley. Central Sulawesi, Indonesia. Five mice were naturally infected through subcuian with 75 cercariae of Schistosoma japonicum emerged from snail, Oncomelanic hupensis lindoensis, which were found in the Napu valley, Central Sulawesi. Mice showed a weakness, anorrhexia, stomach ache,and died in the fifth, sixth and seventh week posiinfection. At autopsy, mice had ascites, yellowish nodules on the surface of lung, liver, serosa of the small intestine, and mesenterium. Histolagically, there were severe necrosis and the granolomatous inflammation of the liver, the spleen, the lung, the pancreas, and the small intestine.
THE EFFECT OF FEVER ON ACETYLATION RATIO AND SULFADIAZINE ELEMINATION KINETICS OF LOCAL SHEEP (Ovis aries) Ida Tjahajati; Subronto Prodjoharjono; Budiono Santoso
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8621

Abstract

A Study has been conducted to identify effect of fever towards acetylafion ratio and eleminatkm kinetics of sulfadiazine in sheep. The siudy consist 10 healthy sheep using cross­over design. Each animal was treated twice with 40 days interval. In the first treatment the sheep were given 2 gram of sulfadiazine and 0.4 gram pyrimethamine. In the second treatment the animals were treated fever induction prior to treatment using 2 gram of sulfadiazin and 0.4 gram pyrimethamine. Blood samples were taken From jugular vein in hour of 0, 0.08.0.25, 0.5, 1,2,4,6,9.12, 18, and 24. Plasma sulfadiazine levels were analyzed using Bratton-Marshall Methode. Sulfadiazin parameter was calculated using Stripe program. Theresults of the study revealed that fever did not significantly (P>0,05) increased the aceiylation ratio and inhibited elemination of sulfadiazine.
INTRA-UTERIN ADMINISTRATION OF PROSTAGLANDIN FOR INDUCTION IN CROSS BREEDING GOATS Sri Gustari; Asmarani Kusumawati; Slamet Subagyo; Prabowo P Putro
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8622

Abstract

Productivity and reproductivity of cross breeding goats is low. It is mainly caused by hormone deficiency and difficulties in estrus detection. The research studied the effect of intrauterine administration of prostagiandin on estrus induction and on number of pregnancies.Sixteen does were divided randomly into 2 groups. Group i were injected with prostagiandin intramuscularly. Group li were given prostagiandin by intrauterine administration. In both groups, prostagiandin were given twice with interval 11 days apart. The does which coming into estrus were mated naturally.There was a significantly different in the time of estrus between group I and group II (40.25 ± 4.94 and 80.8 ± 18.35 hours). On the other hand, there was no difference in estrus induction between those two groups (Group I : 87.5% and Group II : 62.5 %). All the does which were mated naturally were pregnant (100%). Although time to estrus after intrauterine administration of prostagfandin was longer than that of intramuscular ones, but it was a cheaper method.
A STUDY OF PREPATENT PERIODES, DEATH TIMES AND REDUCTION OF BODY WEIGHT IN EXPERIMENTAL RATS (RATTUS NORVEGICUS) INFECTED WITH TRYPANOSOMA EVANSI Sumartono Sumartono; Eryl Sri Rohayati
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8623

Abstract

The prepatent periods of T. evansi, death times and reduction of foody weights of experimental rats (Rattus norvegicus) infected with T. evansi were studied.Twelve rats were divided into four groups (group 1-!V), each group contains of three rats. The rate of the group II, group III and group IV, were infected intraperitonealy with 300, 600 and 2400 T. evansi per rat, respectively. The group I was used as a control group. After infection, their body weight were measured, followed by blood examination and detection of death time everyday.The data were collected then analysed descriptively using regression-correlation between total number of T. evansi wbich were infected, prepatent period, death time and body weight changes.The results of the experiment indicated that the higher dose of T. evansi infected, caused shorter prepatent period and death time, but there were a negative correlation between total number of T. evansi infected and percentage of body weight reduction. The evidence also showed even thought the prepatent period was shorter, all experimental rats died within 5-6 days after T. evansi detected in the peripheral blood.
COLIBACILLOSIS IN BURAS CHICKENS IN SOUTH KALIMANTAN (KOLIBASILOSIS PADA AYAM BURAS DI KALIMANTAN SELATAN) Bambang Nganji Utomo
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8624

Abstract

Thirty eight buras chickens of various ages with clinical signs diseases of illness were diagnosed for colibacillosis and other diseases, The diagnoses were carried out by isolation and identification of causative agents, pathological and histopathological examination. The isolated E. coli were tested serologically and their sensitivity towards antibiotics and sulfouamid.The results indicated that E. coli were isolated from 42.11 % chickens, either as single infection or in the connection with Newcastle Disease, salmonellosis, coccidiosis, aspergillosis, pasteurellosis aad mycoplasmosis. Serological test indicated that 25% of E. coli isolates belong to serotype 0,2 : KI and 18.75% serotype 078 : K80. The Sensitivity test showed that 73.33% of E. coli isolates were resistant to the antibiotics and sulfonamid, where as 26.67% were sensitive.
RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS R. Wasito; Hastari Wuryastuti
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8625

Abstract

Skunks experimentally infected with street virus rabies intramuscularly were used to demonstrate rabies viral antigen in paraffin-embedded tissue by immunopathology diagnosis approach, the avidin biotin complex (ABC) method. The use of ABC method provides a simple and sensitive method to localize rabies virus antigen in formalin- fixed tissues. The results of the present study also demonstrated that a procedures using formalin-fixed parafin-embeded tissue and stained immunopathologicalH by the ABC method was an excellent method for both preservation of morfological details of ceils/tissues and demonstration of rabies virus antigen.
RADIO RECEPTOR ASSAY FOR DIAGNOSTIC SERUM AND MILK 1,25-DIHYDROXYCHOLECALCIFEROL Hastari Wuryastuti
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8626

Abstract

The objectives of this study were to modify and develop technique for determination of 1,25-(OH)2D3 concentrations in sera and milk samples. The developed technique, used non-HPLC for extraction and purification of the samples and involved radio receptor assay which used calf thymus as 1,25-{OH)2Dj receptor for quantitation of 1,25-(OH)2D3 Results of this study were compare to that of the technique developed by Horst et at. (1981) which used HPLC for samples extraction and purification. The correlation between the two techniques was r=0,95. Using the modified technique the 1,25-(OH)2D3 concentrations in cow and sow sera were 57,42 ±5,3 pg/ml (n=20) and 75.22 ±6.05 pg/ml (n=20) respectively, whereas the laSHOH^D., concentrations in cow and sow milk were 16.62 ±2.2 pg/ml (n=20) and 21.52 + 3.125 pg/ml (n=20). Based on the results could be concluded that the technique that had been developed in this study was found satisfactory for determination the l,25-(OH)2Dj concentration either in serum or milk samples.
DYNAMICS OF NUMBER OF NEMATODE LARVAE IN GRASS IN THE PASTURE Joko Prastowo; Sumartono Sumartono
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8641

Abstract

The existence of nematode larvae in a pasture can be used as an indication of infection or reinfection in animal grazing on it. The objective of the current study was to reveal the dynamics of the number of nematode larvae found on the grass of a pasture.Three locations of the pasture of the teaching farm of Faculty of Veterinary Medicine GMU, were chosen as sampling areas. Grass of the areas was cut just on top of the soO five times a day for ten day namely at 5, 6-9 am - 1 pm, 2-5 pm and 6 pm. Following cutting, the grass was kef t m a tube and washed with detergent solution. The washing solution was examined under a stereomicroscope, and the larvae observed were counted. Differences in the number of larvae between locations and collection times were analysed statistically by names of factorial analysis of variance.The results show that the mean number of larvae varies between 03 ± 0.67 and 6.1 ± 2.95 per grass. The number of larvae is not significantly different (F > 0,05). Cutting at however, is influenced by collection time (P < 0,05). Cutting at 5-9 am gives the largest number of larvae, while 6 pm gives the lowest count.  '
THE EFFECT OF CADMIUM CHLORIDE TO THE STERILITY OF MALE RAT Syarifuddin Tato; Puspa Wikan Sari
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8642

Abstract

The effect of cadmium chloride (CdO2) to the sterility of male rat have been observed to the first generation (Fj).Sixty white rats consisted of 30 male rats at 3 weeks old divided Into 6 groups and were given CdCl2 orally ad libitum for 42 days, Tl»e concentration of CdCl2 for each group was 0 ppin (group I), 200 ppm (group H), 100 ppm (group ID), 50 ppm (group IV), 25 ppm (group V) and 12,5 ppm (group VI). After treatments, aii male rats were mated with female rats by mixing a couple of rats in a cage for 7 days. Then male rats were kiiied all to examine the histopathoiogy of their testis. The female rats were kept until 18 days of pregnancy, then all were killed. The percentage of pregnancy and the number of baby in each female rat were counted. The histopamoiogy of seminiferous tubules of testis were analysed qualitatively.The results showed that CdCl2 at 12,5 and 25 ppm caused 20% and 40% of infertility. 50 ppm of CdCfe caused 100% of infertility and necrosis of spermatocyte and spermaride celk; there no spematozoa in the seminiferous tubules.

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