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Current Biomedicine
Published by Institut Pertanian Bogor
ISSN : 29628490     EISSN : 29854784     DOI : https://doi.org/10.29244/currbiomed
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
Aim. Current Biomedicine aims to publish scientific article in the biomedical fields. Scope. Current Biomedicine will publish widely relevant topic in the field of biology (life science), directly or indirectly, support the improvement of human health. These fields include, but are not limited to: anatomy, physiology, histology, embryology, genetics, pharmacology, toxicology, pharmacy, parasitology, pathology, microbiology, clinics, radiology and imaging, surgery, experimental surgery, reproduction, ethnomedicine, phytopharmaceuticals, biotechnology, biomedical engineering, bioinformatics, public health, epidemiology, legislation and bioethics, and one health.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2 Documents
 1 
Search results for , issue "Vol. 4 No. 1 (2026): January" : 2 Documents clear
Detection of quinolone antibiotic resistance genes in Escherichia coli isolated from dairy cattle feces Raihan, Muhammad Ammar; Elsharkawy, Sara; Indrawati, Agustin; Latif, Hadri
Current Biomedicine Vol. 4 No. 1 (2026): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.4.1.1

Abstract

Background Livestock raised in densely populated areas can serve as reservoirs for bacteria such as Escherichia coli, which may harbor antibiotic resistance genes that threaten both animal and human health. Objective This study aimed to identify and characterize quinolone resistance genes in E. coli isolated from dairy cattle feces. Methods Fifteen E. coli isolates were obtained from 15 dairy farms located in Kebon Pedes, Bogor, West Java. Genotypic detection of quinolone resistance genes was conducted using DNA sequencing on the MinION platform. Results All E. coli isolates (100%) carried at least one quinolone resistance gene. Of these, ten isolates (67%) contained a single resistance gene, while five isolates (33%) possessed two genes. The qnrS1_1 gene was identified in all isolates and represented the predominant genotype, whereas the qnrVC4_1 gene was found in five isolates (33%), mostly co-occurring with qnrS1_1. Both genes are plasmid-mediated and categorized as plasmid-mediated quinolone resistance (PMQR) genes. Conclusion The detection of qnrS1_1 and qnrVC4_1 genes in E. coli isolated from dairy cattle feces indicates that livestock manure may act as a reservoir for quinolone resistance genes, contributing to their persistence and potential spread within farm environments.
Preparation of avian influenza H5N1 and Newcastle disease antigens for hemagglutination inhibition assay applications Poetri, Okti Nadia; Salsabila, Syaharani; Pebriana, Nada Adinda
Current Biomedicine Vol. 4 No. 1 (2026): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.4.1.8

Abstract

Background Avian influenza (AI) and Newcastle disease (ND) are major poultry diseases in Indonesia, where monitoring of vaccination efficacy commonly relies on the hemagglutination inhibition (HI) assay. The HI assay requires viral antigens, which are generally obtained commercially from PUSVETMA. However, independent antigen preparation would be beneficial for private laboratories. Objective This study aimed to prepare and validate in-house AI and ND antigens as HI assay reagents. Methods Viruses were propagated in embryonated chicken eggs (ECE), inactivated using buffered neutral formalin (BNF), precipitated with polyethylene glycol (PEG-6000), and preserved with glycerol. Validation was conducted by parallel HI testing of 24 chicken sera for AI and 22 chicken sera for ND using both in-house and PUSVETMA’s antigens. Antibody titers were analyzed using analysis of variance (ANOVA), with sensitivity (Se), specificity (Sp), and kappa (κ) tests performed for agreement.   Results ANOVA revealed no significant differences in geometric mean titers between in-house and PUSVETMA’s antigens (P<0.05). Both the AI and ND in-house antigens demonstrated Se and Sp values of 100% and κ values of 1, indicating perfect agreement. Conclusion These findings confirm that in-house AI and ND antigens are comparable to their commercial counterparts and can serve as reliable and cost-effective reagents for HI testing in private laboratories.

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