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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Berkala Penelitian Hayati Jurnal Kedokteran Hewan Jurnal Farmasi Indonesia Current Biomedicine Policy Brief Pertanian, Kelautan, dan Biosains Tropika

Okti Nadia Poetri

IPB University

Author-ID : 2480503

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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PRODUKSI ANTIBODI KUNING TELUR (IgY) ANTI STREPTOCOCCUS MUTANS SEBAGAI ANTI KARIES GIGI Okti Nadia Poetri; Retno D Soejoedono
Jurnal Ilmu Pertanian Indonesia Vol. 11 No. 3 (2006): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.712 KB)

The aim of this study was to explore IgY anti Streptococcus mutan production and the ability of Igy Streptococcus mutans blocking adhesion process. The eggs was collected from Single Comb Brown Leghorn which have been immunized by S. mutan. Agar gel precipitation test was done to detect IgY anti S. mutans in serum and egg. Egg which Countain IgY anti S. mutans was collected. IgY anti S. mutans extracted from egg yolk by mean s PEG-Amonium sulfat and purified using fast protein liquid chromatography. The purity of Igy anti S. mutans was determined by UV spectropometer. Biological activities of Igy anti S. mutans to inhibit adhession process was learned by anti adhesion test. We use two dose of IgY, which is 100 ug and 500 ug. Igy anti S. mutans formen in serum five weeks after the first immunization while it formed in egg nine weeks after the first immunization. Igy anti S. mutanss still present in serum andegg until twelve weeks from the first immunization. Igy anti S. mutanss could decrease the amount of bacteria which attach the epithelial cell surface. The amount of sticky bacteria on epithelial cell (without IgY) are 40 cell bacteria/epithelial cell. After blocked by IgY anti S. mutanss the amount of bacteria turn into 30 cell bacteria/epithelial cell (for dose of 100 ug IgY) and 28 cell bacteria/epitheelial cell (for dose of 500 ug IgY). This research concluded that hens were capable producing IgY anti S. mutanss in egg yolk and it can be used to solve dental caries problem which caused by S. mutanss.

Preparasi Imunoglobulin Yolk (IgY) Spesifik Virus Rabies untuk Pengembangan Kit Diagnostik Suwarny Ruhi; Sri Murtini; Okti Nadia Poetri; Retno D Soejoedono
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Penelitian ini bertujuan untuk memproduksi dan mengkarakterisasi IgY anti rabies sebagai bahan diagnostik. Ayam petelur usia produktif divaksinasi dengan vaksin rabies inaktif secara parenteral melalui rute intramuskular dengan dosis 0,5 ml sebanyak 2 kali. Keberadaan IgY pada telur dievaluasi dengan metode ELISA. Konsentrasi total protein IgY di hitung dengan metode Bradford. IgY dipurifikasi menggunakan dua metode yaitu : 1) pengendapan dengan NaCl, PEG 6000-amonium sulfat; 2) teknik Water Soluble Fraction (WSF), dilanjutkan pengendapan dengan PEG 6000-amonium sulfat. Titer IgY spesifik di tentukan dengan uji ELISA dan karakterisasi protein dengan metode SDS-PAGE. Hasil pengujian menunjukkan, antibodi anti-rabies dapat dideteksi pada kuning telur di minggu kedua setelah vaksinasi pertama. Purifikasi IgY dengan NaCl menghasilkan konsentrasi 331 µg/ml dan teknik WSF 184 µg/ml. Karakterisasi protein pada teknik NaCl menghasilkan 6 pita protein dengan berat molekul 164,16 kDa, 126,43 kDa, 97,36 kDa, 68,73 kDa, 40,76 kDa, 28,77 kDa sedangkan teknik WSF hanya terdiri dari 3 pita dengan berat molekul 94,03 kDa, 65,61 kDa, dan 31,94 kDa. Titer antibodi spesifik menggunakan teknik NaCl lebih besar dari 0,5 IU/ml dan teknik WSF dengan titer antibodi di bawah 0,5 IU/ml . Berdasarkan hasil penelitian, dapat disimpulkan bahwa IgY spesifik rabies dapat diproduksi pada ayam petelur dan menghasilkan titer antibodi ≥ 0,5 IU/ml, dengan titer antibodi spesifik rabies sebesar ≥ 0,5 IU/ml.

MP-8 Lack of Antibody Formation Against Inactivated Avian Influenza Virus in Ducks and Chickens After Intranasally Immunization Okti Nadia Poetri; Retno Damayanti Soejoedodo; Ni Luh Putu Ika Mayasari; Ekowati Handharyani; Novera Nirmalasanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.484 KB)

Vaccination is one of control strategies implemented in endemic countries such as Egypt and Indonesia [1,2]. Most commercial AI vaccines available in Indonesia are adjuvanted inactivated AI vaccines applied through intramuscular routes. Vaccine application by subcutaneous or intramuscular injection can cause pain and stress in poultry, the route of vaccine through the nasal drip (intranasal) is a more convenient and painless. However, respiratory applied inactivated influenza is poorly immunogenic. Therefore prior to developing inactivated intranasal vaccine, it is necessary to study antibody response to inactivated AI virus which exposed through intranasal route. The aim of our research was to determined antibody response of ducks and chickens against avian influenza virus (AIV) subtype H5N1 after intranasally immunization.

AQ-9 Identification of Sumateran Wild Boar Meat (Sus scrofa vittatus) by Restriction Fragment Length Polymorphism (RFLP) Analysis of Cytochrome b Gene Melani Wahyu Adiningsih; Retno Damayanti Soejoedono; Trioso Purnawarman; Hadri Latif; Rahmat Setya Adji; Okti Nadia Poetri; Dwi Desmiyeni Putri
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.42 KB)

Sumateran wild boars have been super abundant in Sumateran forest. In Indonesia, this wildlife condition has led to the exploitation for commercial purpose. The high number of Sumateran wild boars population increases wild boar hunting resulting in an abundant availability of wild boar meat in the food market with extremely cheap price. The macroscopic similarity of wild boar meat and beef has prompted the local people to abuse this situation by selling wild boar meat in traditional market as beef. Based on annual record from Cilegon Class II Quarantine Office in 2014, there were nine smuggling cases or a total of 21.556 kg of wild boar meat smuggling effort that were prevented by Cilegon Quarantine officers. The number of food safety concerns related to smuggling of wild boar or counterfeiting beef with wild boar is a very detrimental condition for consumers, especially consumers in traditional markets.The checking of genuineness or validity of food products is an important effort to protect people from consuming unhealthy food and to indicate whether the food is halal or not. Studies of meat detection should be continuously developed as an effort to protect consumers. Genetic method is the most specific and sensitive method to check food ingredients authenticity by detecting the presence of genetic material or deoxyribonucleic acid (DNA). It results from the specific character of the structure of DNA particles and the possibility of using the information included in them. The most frequent loci used for species identificationin phylogenetics and biodiversity studies are mitochondrial cytochrome b (cyt b).Genetic method is the most specific and sensitive tool for analyzing the authenticity of food ingredients in a molecular level by means of detecting the presence of genetic material or deoxyribonucleic acid (DNA). One of the various methods could be used to detect genetic material is polymerase chain reaction (PCR). Specifically, one of such method frequently used in food industry to observe animal derived product fabrication is PCR restriction fragment length polymorphism (RFLP). PCR-RFLP is based on the comparison of the bands profile generated after certain enzymes digest the DNA target. PCR-RFLP is appropriate for meat testing due its ability in exploiting sequence variation in designated DNA region that allows species differentiation even from closely related species through DNA fragment restrictions selected by suitable restriction enzyme. PCR-RFLP is advantageous since it is simple, cheaper, and easier to be adjusted for routine big-scale studies such as surveillance program.

PERAN ANTIBODI KUNING TELUR (IgY) SEBAGAI OPSONIN UNTUK PENCEGAHAN SERANGAN MUTAN STREPTOCOCCUS SEROTIPE D (STREPTOCOCCUS SOBRINUS) Okti Nadia Poetri; Retno D. Soejoedono; Agustin Indrawati; I Wayan T. Wibawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/359

The aim of this study was to explore the role of serotype d Mutan Streptococcus (Streptococcus sobrinus) spesific immunoglobulin Y (IgY-Ss) as opsonin against the same strain. The eggs were collected from Single Comb Brown Leghorn which has been immunized with Streptococcus sobrinus. Agar gel precipitation test was applied to detect IgY-Ss in serum and egg. Egg containing IgY-Ss was collected and extracted by PEG-Amonium sulphate and purified using fast protein liquid chromatography. The purity of IgY-Ss was determined by UV spectrophotometer. Molecular weight was established by SDS-PAGE (sodium dedocyl sulphate-poly acrilamide gel electrophoresis). Biological activities of IgY-Ss as opsonin was determined by phagocytosis assay. Phagositic activity of macrophages was not increased by preincubation of both S. sobrinus (107 CFU/ml) and 100 μg of IgY-S, however the phagositic capacity was increased from 1.6 bacterial cell/ macrophag to 5.17 bacterial cell/ macrophag. These finding suggest that IgY-Ss obtained from hens immunized with S. sobrinus provide an alternative to prevent S. sobrinus infection.

THE POTENTIAL OF ADJUVANT AGAINST PRODUCTION OF ANTISTREPTOCOCCAL IMMUNOGLOBULIN Y (IGY) IN AQUACULTURE Rifky Rizkiantino; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Retno Damajanti Soejoedono; Okti Nadia Poetri; Wyanda Arnafia; Kris Damar Sasi; Dinda Reisinta
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

This study was conducted to explore the potential of adjuvant for the production of immunoglobulin Y (IgY) as antistreptococcosis in layer chicken with mass production orientation. Enterococcus faecalis which causes streptococcosis in the red tilapia was selected as a candidateantigen. The production of immunoglobulin Y (IgY) was carried out on Isa Brown layer chickens and aged around 20 weeks. Furthermore, thechickens were grouped into four groups (A, B, C, and D groups), each consisting of three chickens based on the type of adjuvant, while twochickens were used as a control group. Each group was treated by giving MONTANIDE™ ISA 71R VG adjuvant (A), Freund's adjuvant (B), aluminum potassium sulphate adjuvant (KAl(SO4)2∙12H2O) concentration of 50 ppm in pH 7 (C), and only antigens without adjuvant (D). Chickens were kept for 35 days and each week was checked for presence the IgY antigen in the serum and egg yolk. Booster was conducted on 14th and 28th days of maintenance. The results showed that IgY in treatment group A was detected on day 28 in the serum and day 35 in the yolk. Whereas the treatment group B could be detected on day 35 in the serum. However, the IgY was not detected in the serum and yolk in C, D, and control groups until the end of the maintenance. Based on the results, it can be concluded that the appearance of IgY in serum and yolk in a relatively fast time is obtained in the combination of Enterococcus faecalis antigen with the emulsion of water-in-oil adjuvant (SEPPICMONTANIDE™ ISA 71R VG) compared to the other types of adjuvant that use in this study.

Tanggap Antibodi terhadap Capsid Virus Penyakit Jembrana setelah Vaksinasi Lapang Sapi Bali di Kabupaten Sarolangun, Jambi Ferry Ardiawan; Okti Nadia Poetri; Nur Khusni Hidayanto; Ari Rumekso; Dilas Pradana; Surachmi Setiyaningsih
Acta VETERINARIA Indonesiana Vol. 11 No. 2 (2023): Juli 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.2.167-174

Penyakit Jembrana (JD) adalah penyakit prioritas nasional yang disebabkan oleh infeksi lentivirus pada sapi Bali. Pemerintah merekomendasikan vaksinasi sebagai langkah penting pengendalian di wilayah endemis. Penelitian ini bertujuan untuk mempelajari dinamika tanggap antibodi dengan melakukan kajian kohort sejalan dengan program vaksinasi JD di Desa Pematang Kabau oleh Dinas Peternakan dan Perikanan Kabupaten Sarolangun. Sera dari 36 sapi Bali pendatang, yang menerima dua dosis vaksin JD-VetÂ® dengan selang waktu 30 hari, dikoleksi pada hari 0, 44, dan 90. Antibodi spesifik kapsid virus JD (JDV) diukur menggunakan metode ELISA. Sebelum vaksinasi, 88,89% sapi menunjukkan hasil seronegatif, sedangkan 11,11% menunjukkan seropositif yang menandakan adanya paparan JDV terdahulu. Tidak ada reaksi membahayakan yang diamati pada sapi yang divaksinasi. Vaksinasi menurunkan titer antibodi secara signifikan pada sapi seropositif. Sebaliknya, 71,87% sapi seronegatif menunjukkan respons positif, meskipun hanya 40,63% yang mencapai tingkat seroprotektif pada hari ke-44. Persentase ini menurun secara signifikan menjadi 15,63% pada hari ke- 90, mengindikasikan durasi kekebalan yang relatif pendek. Temuan ini menggarisbawahi pentingnya mempertimbangkan status kekebalan pravaksinasi dan menegakkan pengendalian lalu-lintas sapi Bali. Meskipun vaksin JD-VetÂ® terbukti aman, namun mempertahankan kadar antibodi yang tinggi masih menjadi tantangan. Kajian lebih lanjut sangat diperlukan untuk mengoptimalkan strategi vaksinasi dan meningkatkan pengendalian JD pada sapi Bali.

COMPARATIVE ANALYSIS OF PROBING AND CONTRAST RADIOGRAPHY FOR GENDER DETERMINATION IN RETICULATED PYTHONS (Malayopython reticulatus) Tan, Zhi Yien; Poetri, Okti Nadia; Ulum, Mokhamad Fakhrul
BIOTROPIA Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.3.2278

Article Highlights- Accurate gender identification supports effective breeding programs and ensures snakes' welfare.- This study emphasizes that probing is more reliable and efficient than contrast radiography for gender determination in reticulated pythons.- The probing method proved to have 100% accuracy in determining the gender of reticulated pythons, outperforming contrast radiography, which only achieved 75% accuracy. AbstractReticulated pythons, like most snakes, do not exhibit noticeable sexual differences, which makes gender determination necessary. Probing and contrast radiography were chosen as methods to determine sex and to compare their accuracy. Six reticulated pythons with body lengths of 128-167 cm, tail lengths of 5.4-9.6 cm, and body diameters of 1.4-3.4 cm were examined twice by probing and contrast radiography. The results showed that probing had 100% accuracy, with probe lengths ranging from 1.37-3.60 cm for male snakes and 0.3-0.76 cm for female snakes. Contrast radiography had 75% accuracy, with the hemipenis appearing as a white silhouette and female snakes appearing colorless. The accuracy rates highlight the effectiveness of probing over contrast radiography for reliable gender determination in reticulated pythons

Formulasi Sabun Cair Perak Nanopartikel dengan Penstabil Polyvinil Alcohol : Sabun Cair Perak Nanopartikel Sutriyo; Hanandi, Sharon; Putri, Kurnia Sari Setio; Poetri, Okti Nadia; Annisa, Syifa; Rahmasari, Ratika
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 13 No 1 (2021): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

According to Global Burden of Disease reported on 2019, about 1.53 million deaths caused by infectious diseases such as pneumonia and diarrhea. Triclosan is one of the active ingredient commonly used in antibacterial soap as one way to prevent the spread of infectious disease.. However, bacteria resistance against triclosan has been reported. Silver nanoparticles (AgNP) is an alternative antibacterial that potential to be used in liquid hand wash. However AgNp tend to aggregate during storage, thus stabilizer is needed This study aims to synthesize AgNP, formulate the liquid hand wash contain AgNP with polyvinil alcohol as stabilizer, and evaluate its effectiveness against Escherichia coli, Staphylococcus aureus, and Salmonella thypi. AgNP was prepared using the chemical reduction method between silver nitrate and sodium borohydride, followed by its characterization using UV-Vis spectrophotometer, TEM, PSA, and AAS. The physical characteristic of AgNp-liquid hand wash were also evaluated. Further, the antibacterial activity of AgNP-handwash was evaluated by phenol coefficient method. The peak of UV absorption spectrum of colloidal was found at 404.2 nm indicated the presence of AgNP. Ag content in AgNP colloidal was 38.405 mg/Kg ± 0,008. The spherical shape of AgNP was observed. The AgNP size was 65.1 nm with polydispersity index value of 0.543, and zeta potential value was -22.25 mV. The obtained AgNP-hand wash met the Indonesian standard criteria and was stable for 28 days. The best phenol coefficient value was obtained at formulation with addition of 30% AgNP (0.1 for S. typhi, 0.4 for E. coli, and 0.01 for S. aureus).

Formulasi Nasal Spray Anti-Influenza yang Mengandung Nanopartikel Perak : Nasal Spray Anti-Influenza Nanopartikel Perak Sutriyo; Wilbert Wylie; Kurnia Sari Setio Putri; Okti Nadia Poetri; Rahmasari, Ratika
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 13 No 2 (2021): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Influenza A virus is one of the most common causes of respiratory disease in the world. Even though, vaccines and anti-influenza virus are become the first line for therapy, but the mutation ability of influenza virus is able to cause several outbreaks in the world. Silver nanoparticles (AgNP) have been proven to exhibit antiviral activity; however, the use of AgNP in pharmaceutical products is still limited. In this study, we aimed to formulate nasal spray containing AgNP, to evaluate its physicochemical properties, and its antiviral activity toward H5N1 influenza A virus. AgNP were synthesized using chemical reduction method with polyvinyl alcohol as stabilizer, and further prepared into nasal spray product. Physicochemical properties and anti-hemagglutination activity of nasal spray were further evaluated. The nasal spray contained different size of AgNP (less and more than 50 nm) showed physical stability after 28 days storage. However, Anti-influenza evaluation of nasal spray contained AgNP less than 50 nm exhibited better anti-hemagglutination activity against influenza A virus.

Co-Authors Agustin Indrawati Aldo Yanuar Wuriyantara Anindita Sista Widyadhari Anindita Sista Widyadhari Annisa, Syifa Apriana, Cut Desna Ari Rumekso Cece Sumantri Chaerul Basri Dilas Pradana Dinda Reisinta Dwi Desmiyeni Putri Dwi Utari Rahmiati Eko Sugeng Pribadi Ekowati Handharyani Etih Sudarnika Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fathurrahman Fathurrahman Ferry Ardiawan Hadri Latif Hanandi, Sharon I Wayan T. Wibawan I wayan Teguh Wibawan Kal Alfin, Zabrino Wahid Kalsum, Salsabila koekoeh santoso Kris Damar Sasi Kurnia Sari Setio Putri Kurnia Sari Setio Putri, Kurnia Sari Setio Kuswanti, Andrea Septiandra La Jumadin La Jumadin Melani Wahyu Adiningsih Mokhamad Fakhrul Ulum, Mokhamad Ni Luh Putu Ika Mayasari Novera Nirmalasanti Nugraheni, Indi Amalia Nur Khusni Hidayanto Nurjumaatun Nurjumaatun Pebriana, Nada Adinda Pramuwidyatama, M. Gumilang Putra, Raditya Pradana R. Dikky Indrawan Rahayu WoroWiranti Rahmasari, Ratika Rahmat Setya Adji Rakhmat, Asep Reisinta, Dinda Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Rifky Rizkiantino Rizkiantino, Rifky Rong, Chin Xiao Salsabila, Syaharani Sasi, Kris Damar Setyawati, Yusefin Marcelina sri murtini . Surachmi Setiyaningsih Sutriyo Suwarny Ruhi Tan, Zhi Yien Trioso Purnawarman Widyadhari, Atsmarina Wilbert Wylie Wyanda Arnafia Wyanda Arnafia, Wyanda

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