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Contact Name
Hartalina Mufidah
Contact Email
hartalina@uds.ac.id
Phone
+6285728167096
Journal Mail Official
hartalina@uds.ac.id
Editorial Address
Jl. DR. Soebandi No.99, Cangkring, Patrang, Kec. Patrang, Kabupaten Jember, Jawa Timur 68111
Location
Kab. jember,
Jawa timur
INDONESIA
Journal of Medical Laboratory in Infectious and Degenerative Diseases
ISSN : -     EISSN : 30325447     DOI : https://doi.org/10.36858/jmid.v1i2
Journal of Medical Laboratory in Infectious and Degenerative Diseases is a scientific journal with the scope of research conducted by lecturers, practitioners, and students. The purpose of publishing this journal is to accommodate scientific writings that have the substance of research activities, which are the downstream results of research carried out based on an analysis of the needs of the community for scientific and technological contributions in the fields of Parasitology, Bacteriology, Virology, Hematology, Clinical Chemistry, Immunology, Molecular Biology, Cytohistotechnology, Micology, Clinical Toxicology, and Degenerative Diseases.
Articles 5 Documents
Search results for , issue "Vol. 1 No. 1 (2023): December" : 5 Documents clear
Test For Antibacterial Activity Of Green Bean Extract (Vigna Radiata L.) Against Bacteria Escherichia Coli Using Well Diffusion Method Agustin, Rizky Amalia Eka
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v1i1.4

Abstract

Background: Mung beans (Vigna radiata L.) bioactive components that known antibacterial but for their benefits not widely known. This plant includes VIMA 5 mung bean type which the most common variates. Bacteria digestive tract infections Escherichia coliPurpose: to determine antibacterial activity of mung bean extract against bacteria that commonly infect digestive tract,namely Escherichia coli.Methods: Design of this research experimental laboratory using sokhletasi method with ethanol solvent. Sample used ethanol extract mung bean (Vigna radiata L.) concentration dilutions of 10%, 20%, 30% 40%. The positive control used chloramphenicol and negative control DMSO. Inhibition test using the well diffusion method with a diameter of 6 mm. Mung bean compounds contain bioactive components such as flavonoids, tannins, saponins and triterpenoids.Results: The test results show that mung bean seeds have antibacterial activity against Escherichia coli bacteria characterized by the presence of inhibition zones obtained on average for 10% concentration (11.04 mm), 20% concentration (12.04 mm), 30% concentration (12.83 mm), and 40% concentration (13.88 mm) where the whole is classified in the strong category.Conclusions: It can be concluded that the ethanol extract of mung bean seeds (Vigna Radiata L) has antibacterial activity.
Antibacterial Activity Test Of Aloe Vera (Aloe Vera) On The Growth Of Bacteries Propionibacterium acnes Candra Ratna Kumala, Ravika
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: Acne is a complex disease with elements involving bacterial growth and immunity. One of the bacteria that can cause acne is Propionibacterium acnes bacteria. Aloe vera contains compounds that can inhibit bacterial growth such as saponins, flavonoids, alkaloids, and anthraquinones.Purpose: This study aims to determine the presence of antibacterial activity and antibacterial differences in 70% ethanol and ethyl acetate extracts of aloe vera meat against Propionibacterium acnes.Methods: This type of research is experimental using the maceration extraction method with 70% ethanol and ethyl acetate solvents. The sample of this research is aloe vera meat extract with a concentration of 75%, 50%, and 25%. The positive control used was tetracycline antibiotic and the negative control used distilled water. The antibacterial activity test used the pitting diffusion method with millimeter (mm) measurement.Results: The test results showed that there was antibacterial activity for ethanol extract and ethyl acetate extract. The ethanol extract formed the highest inhibition zone with a diameter of 6.77 mm at a concentration of 75%, for a concentration of 50% obtained 5.56 mm, and a concentration of 25% obtained 5.19 mm. While the ethyl acetate extract formed the highest inhibition zone with a diameter of 4.53 mm at a concentration of 75%, for a concentration of 50% obtained 3.45 mm, and a concentration of 25% obtained 2.47 mm. And there are differences in activity in the two extracts, namely 75% ethanol extract is different from 50% and 25% ethyl acetate extract, and vice versa.Conclusions: So it can be concluded that ethanol extract and ethyl acetate extract of aloe vera meat have antibacterial activity against Propionibacterium acnes. And there is a difference in antibacterial activity in 75% ethanol extract and 50% and 25% ethyl acetate extract. 
Detection of Escherichia coli Bacterial Contamination on Cutlery at Warung Lesehan Around Nologaten Yogyakarta Utami, Hanifah Viki Tri
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: Diarrhea is among the second-highest disease cases in the Special Region of Yogyakarta. Escherichia coli infection most often causes diarrhea. Escherichia coli infection can be through food and drinks with low hygiene and sanitation. Escherichia coli bacterial contamination indicates hygiene and sanitation in food preparation.Purpose: This study aims to detect Escherichia coli contamination on cutlery at Warung Lesehan around NologatenYogyakarta.Methods: This type of research is qualitative, with a cross-sectional study design conducted in March-May 2022 in Nologaten Village, Sleman, Yogyakarta. The samples in this study were cutlery in the form of plates, which were determined using an accidental sampling technique of 14 plate samples. Sampling for Escherichia coli examination was carried out using a plate swab technique. The Escherichia coli examination was conducted at the Yogyakarta Health and Calibration Laboratory Center. The results of the research were carried out by univariate data analysis.Results: The results of this study showed that there were 4 (28.6%) positive samples contaminated with Escherichia coli and 10 (71.4%) negative samples contaminated with Escherichia coli.Conclusions: Escherichia coli contamination exists on four cutlery at Warung Lesehan around Nologaten Yogyakarta.
Identification of Health Problems in Ngasem Village Selomartani Kalasan Sleman Yogyakarta 2019 Subagyono
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: Health is the right of every citizen. Problem identification is the first step in determining problem priorities. Kalasan District is one of the sub-districts in Sleman Regency, Yogyakarta which still has health problems, especially Noncommunicable diseases, Healthy Behavior, and environmental.Purpose: This study aims to analyze the factors that cause health problems, especially the problem of non-communicable diseases in the community, and determine the priority of existing problems in Kalasan District, Ngasem Hamlet, Neighbourhood 001, 002, 003, and 004.Methods: This research is an analytical observational study with Community Development Practice study design. The research sample was residents of Ngasem Hamlet RT 001, RT 002, RT 003, and RT 004 totaling 139 families who were determined using accidental sampling techniques. Data analysis was carried out using univariate analysis and priority analysis using the ultrasound method using scoring techniques (Urgency, Seriousness, and Growth).Results: The results of the analysis using the ultrasound method show that the priority problem (I) is hypertension with a total score of 14, the second priority problem (II) is the distance between the Waste Water Management System and the water source <10 m with a score of 10, and the third priority problem (III) is smoking behavior with score 9.Conclusions: It can be concluded that the priority health problems found are hypertension, smoking behavior and the distance of SPAL from water source <10 m.
Level of Knowledge and Attitude of Blue Lagoon Tourism Managers in The Implementation of The COVID-19 Health Protocol Subagyono
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: There were 2,955 confirmed positive cases of COVID-19 in Ngemplak District in 2021, with 98 deaths. Indonesia is in a transitional stage from the pandemic to the endemic. The policy of easing the use of masks causes a decrease in hand hygiene behaviour and a lack of sanitation facilities increasing the risk factors for transmission of COVID-19 in tourist attractions.Purpose: This study aims to determine the management of knowledge and attitudes towards the Blue Lagoon tourism health in implementing health protocols during the transition from the pandemic to the endemic of COVID-19.Methods: This type of research is analytical descriptive research with a cross-sectional approach. The research instrument used a questionnaire with an accidental sampling technique totalling 30 respondents. Data were analyzed by univariate and bivariate.Results: The results showed that 29 people (93.5%) had good knowledge of the respondents with the protocol implementation with a p-value of 0.853. Respondents' attitudes to the protocol implementation showed a positive category of 28 people (90.3%) with a p-value of 0.790.Conclusions: This study concludes that there is no significant relationship between knowledge and attitudes in implementing of the COVID-19 health protocols.

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