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Journal of Medical Laboratory in Infectious and Degenerative Diseases
Published by Universitas dr. Soebandi
ISSN : -     EISSN : 30325447     DOI : https://doi.org/10.36858/jmid.v1i2
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Public Health
Journal of Medical Laboratory in Infectious and Degenerative Diseases is a scientific journal with the scope of research conducted by lecturers, practitioners, and students. The purpose of publishing this journal is to accommodate scientific writings that have the substance of research activities, which are the downstream results of research carried out based on an analysis of the needs of the community for scientific and technological contributions in the fields of Parasitology, Bacteriology, Virology, Hematology, Clinical Chemistry, Immunology, Molecular Biology, Cytohistotechnology, Micology, Clinical Toxicology, and Degenerative Diseases.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 15 Documents
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Test For Antibacterial Activity Of Green Bean Extract (Vigna Radiata L.) Against Bacteria Escherichia Coli Using Well Diffusion Method Agustin, Rizky Amalia Eka
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v1i1.4

Abstract

Background: Mung beans (Vigna radiata L.) bioactive components that known antibacterial but for their benefits not widely known. This plant includes VIMA 5 mung bean type which the most common variates. Bacteria digestive tract infections Escherichia coliPurpose: to determine antibacterial activity of mung bean extract against bacteria that commonly infect digestive tract,namely Escherichia coli.Methods: Design of this research experimental laboratory using sokhletasi method with ethanol solvent. Sample used ethanol extract mung bean (Vigna radiata L.) concentration dilutions of 10%, 20%, 30% 40%. The positive control used chloramphenicol and negative control DMSO. Inhibition test using the well diffusion method with a diameter of 6 mm. Mung bean compounds contain bioactive components such as flavonoids, tannins, saponins and triterpenoids.Results: The test results show that mung bean seeds have antibacterial activity against Escherichia coli bacteria characterized by the presence of inhibition zones obtained on average for 10% concentration (11.04 mm), 20% concentration (12.04 mm), 30% concentration (12.83 mm), and 40% concentration (13.88 mm) where the whole is classified in the strong category.Conclusions: It can be concluded that the ethanol extract of mung bean seeds (Vigna Radiata L) has antibacterial activity.
Antibacterial Activity Test Of Aloe Vera (Aloe Vera) On The Growth Of Bacteries Propionibacterium acnes Candra Ratna Kumala, Ravika
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: Acne is a complex disease with elements involving bacterial growth and immunity. One of the bacteria that can cause acne is Propionibacterium acnes bacteria. Aloe vera contains compounds that can inhibit bacterial growth such as saponins, flavonoids, alkaloids, and anthraquinones.Purpose: This study aims to determine the presence of antibacterial activity and antibacterial differences in 70% ethanol and ethyl acetate extracts of aloe vera meat against Propionibacterium acnes.Methods: This type of research is experimental using the maceration extraction method with 70% ethanol and ethyl acetate solvents. The sample of this research is aloe vera meat extract with a concentration of 75%, 50%, and 25%. The positive control used was tetracycline antibiotic and the negative control used distilled water. The antibacterial activity test used the pitting diffusion method with millimeter (mm) measurement.Results: The test results showed that there was antibacterial activity for ethanol extract and ethyl acetate extract. The ethanol extract formed the highest inhibition zone with a diameter of 6.77 mm at a concentration of 75%, for a concentration of 50% obtained 5.56 mm, and a concentration of 25% obtained 5.19 mm. While the ethyl acetate extract formed the highest inhibition zone with a diameter of 4.53 mm at a concentration of 75%, for a concentration of 50% obtained 3.45 mm, and a concentration of 25% obtained 2.47 mm. And there are differences in activity in the two extracts, namely 75% ethanol extract is different from 50% and 25% ethyl acetate extract, and vice versa.Conclusions: So it can be concluded that ethanol extract and ethyl acetate extract of aloe vera meat have antibacterial activity against Propionibacterium acnes. And there is a difference in antibacterial activity in 75% ethanol extract and 50% and 25% ethyl acetate extract. 
Detection of Escherichia coli Bacterial Contamination on Cutlery at Warung Lesehan Around Nologaten Yogyakarta Utami, Hanifah Viki Tri
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: Diarrhea is among the second-highest disease cases in the Special Region of Yogyakarta. Escherichia coli infection most often causes diarrhea. Escherichia coli infection can be through food and drinks with low hygiene and sanitation. Escherichia coli bacterial contamination indicates hygiene and sanitation in food preparation.Purpose: This study aims to detect Escherichia coli contamination on cutlery at Warung Lesehan around NologatenYogyakarta.Methods: This type of research is qualitative, with a cross-sectional study design conducted in March-May 2022 in Nologaten Village, Sleman, Yogyakarta. The samples in this study were cutlery in the form of plates, which were determined using an accidental sampling technique of 14 plate samples. Sampling for Escherichia coli examination was carried out using a plate swab technique. The Escherichia coli examination was conducted at the Yogyakarta Health and Calibration Laboratory Center. The results of the research were carried out by univariate data analysis.Results: The results of this study showed that there were 4 (28.6%) positive samples contaminated with Escherichia coli and 10 (71.4%) negative samples contaminated with Escherichia coli.Conclusions: Escherichia coli contamination exists on four cutlery at Warung Lesehan around Nologaten Yogyakarta.
Identification of Health Problems in Ngasem Village Selomartani Kalasan Sleman Yogyakarta 2019 Subagyono
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: Health is the right of every citizen. Problem identification is the first step in determining problem priorities. Kalasan District is one of the sub-districts in Sleman Regency, Yogyakarta which still has health problems, especially Noncommunicable diseases, Healthy Behavior, and environmental.Purpose: This study aims to analyze the factors that cause health problems, especially the problem of non-communicable diseases in the community, and determine the priority of existing problems in Kalasan District, Ngasem Hamlet, Neighbourhood 001, 002, 003, and 004.Methods: This research is an analytical observational study with Community Development Practice study design. The research sample was residents of Ngasem Hamlet RT 001, RT 002, RT 003, and RT 004 totaling 139 families who were determined using accidental sampling techniques. Data analysis was carried out using univariate analysis and priority analysis using the ultrasound method using scoring techniques (Urgency, Seriousness, and Growth).Results: The results of the analysis using the ultrasound method show that the priority problem (I) is hypertension with a total score of 14, the second priority problem (II) is the distance between the Waste Water Management System and the water source <10 m with a score of 10, and the third priority problem (III) is smoking behavior with score 9.Conclusions: It can be concluded that the priority health problems found are hypertension, smoking behavior and the distance of SPAL from water source <10 m.
Level of Knowledge and Attitude of Blue Lagoon Tourism Managers in The Implementation of The COVID-19 Health Protocol Subagyono
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

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Abstract

Background: There were 2,955 confirmed positive cases of COVID-19 in Ngemplak District in 2021, with 98 deaths. Indonesia is in a transitional stage from the pandemic to the endemic. The policy of easing the use of masks causes a decrease in hand hygiene behaviour and a lack of sanitation facilities increasing the risk factors for transmission of COVID-19 in tourist attractions.Purpose: This study aims to determine the management of knowledge and attitudes towards the Blue Lagoon tourism health in implementing health protocols during the transition from the pandemic to the endemic of COVID-19.Methods: This type of research is analytical descriptive research with a cross-sectional approach. The research instrument used a questionnaire with an accidental sampling technique totalling 30 respondents. Data were analyzed by univariate and bivariate.Results: The results showed that 29 people (93.5%) had good knowledge of the respondents with the protocol implementation with a p-value of 0.853. Respondents' attitudes to the protocol implementation showed a positive category of 28 people (90.3%) with a p-value of 0.790.Conclusions: This study concludes that there is no significant relationship between knowledge and attitudes in implementing of the COVID-19 health protocols.
Preferences for breeding places for Aedes spp. mosquitoes on materials and location of containers in the working area of Lateri Health Center, Ambon City Akollo, Robi
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.16

Abstract

Background: The mosquitoes Aedes aegypti and Ae. albopictus is a vector of dengue fever in Indonesia. Container characteristics such as material, color, location, shape, volume and water origin influence female Aedes spp mosquitoes in choosing a place to lay their eggs. Mosquito Ae. aegypti prefers artificial containers as a breeding place, while the Ae. albopictus prefers natural containers. Mosquito Ae. aegypti prefers to be indoors while the Ae. albopictus outdoors. Purpose: knowing the preferences for breeding places for Ae mosquitoes. aegypti and Ae. albopictus on materials and location of containers in the Lateri Health Center Working Area, Ambon City. Methods: This research is a descriptive and analytical study with a cross sectional research design. Larvae collection was carried out in Lateri and Lata villages, Lateri Health Center Working Area. Habitat characterization of Ae. aegypti and Ae. albopictus is carried out visually including the location and material of the container. Identification of larvae using the Aedes genus identification key with a microscope. Results: The container materials found at the research location were artificial containers. The basic materials for the containers made in this research are cement, ceramic, plastic, glass, rubber and aluminum. Mosquito larvae Ae. aegypti are more commonly found inside homes while the mosquito larvae of Ae. albopictus is more commonly found outside the home. Conclusions: Mosquito Ae. aegypti and Ae. albopictus uses artificial containers as a breeding place. Mosquito larvae Ae. aegypti are more commonly found inside homes, while Ae. albopictus outdoors
Optimization of the Formula of Hand Sanitizer Preparation of Teak Leaf Extract (Tectona grandis) as an Antibacterial Isnawati, Nafisah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.18

Abstract

Background: One of the major causes of diarrhea is the bacterium Escherichia coli. This bacterium is commonly found on the palms of hands. To address diarrhea, prevention is necessary, one of which is by using ethanol extract of teak leaves (Tectona grandis). Teak leaves (Tectona grandis) contain secondary metabolites such as flavonoids, saponins, tannins, steroids, and triterpenoids that act as antibacterial agents. Purpose: This study aims to determine that the ethanol extract of teak leaves (Tectona grandis) can be formulated into a hand sanitizer with antibacterial properties Methods: The design of this research study is laboratory experimental, with the process of making extracts, formulating them, evaluating the physical quality of preparations, and performing antibacterial tests. The extract was made using the maceration method with 70% ethanol solvent. Hand sanitizer preparations for teak leaf extract (Tectona grandis) are formulated with three concentrations, namely 10%, 12%, and 15%. Results: The results of the organoleptic evaluation showed a medium to dark brown color, with a semi-solid texture and a characteristic teak leaf aroma. The homogeneity test results showed that all formulations were homogeneous. The pH test results were 7.06 for F1, 7.11 for F2, and 7.12 for F3. The spreadability test results were 5.09 cm for F1, 5.42 cm for F2, and 5.14 cm for F3. The adhesion test results were 10.34 seconds for F1, 9.53 seconds for F2, and 12.71 seconds for F3. The viscosity test results were 2,867 cps for F1, 2,567 cps for F2, and 2,611 cps for F3. The antibacterial activity test showed that the hand sanitizer had antibacterial activity with extract concentrations of 10%, 12%, and 15%. The inhibition zone diameters were 12.67 mm for F1, 15 mm for F2, and 21.67 mm for F3. Conclusions: The ethanol extract of teak leaves (Tectona grandis) can be formulated into an emulgel hand sanitizer with antibacterial properties against Escherichia coli, and all three formulations meet the preparation evaluation requirements. The best formula was at a 15% concentration with an inhibition zone of 21.67 mm.
Profile Of SGPT (Serum Glutamic Pyruvic Transaminase) Levels In Active Smokers Aged 20-60 Years Sudarwo, Dimas Taufiqur Rohman; Sholihatil Hidayati; Ahdiah Imroatul M
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.20

Abstract

Background: Cigarettes are one of the main risk factors for several chronic diseases that can result in death. This shows that cigarettes are a big problem for public health. Purpose: to determine the SGPT (Serum Glutamic Pyruvic Transaminase) level profile in active smokers aged 20-60 years Methods: This research is quantitative. The population in this study is active smoking patients at the Grand Raya Husada Clinic, Pandanarum Village, Lumajang Regency, which totals 60 active smoking patients. The data analysis technique used in this study is descriptive. Results: The results of laboratory examinations showed that serum SGPT (Serum Glutamic Pyruvic Transaminase) levels in 60 respondents showed that 55 respondents (91.67%) had normal SGPT levels. As many as 5 respondents (8.33%) showed high or abnormal SGPT levels. Conclusions: Normal SGPT levels in smokers aged 20-60 years were 91.67%, and high or abnormal SGPT levels were 8.33%.
Analysis of the Number of Leukocytes in Dengue Hemorrhagic Fever Patients at Mitra Sehat Situbondo Hospital in 2023 Oktafiyan, Sheli Tiara
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.22

Abstract

Background: Dengue Hemorrhagic Fever is an infectious disease that still poses a significant public health challenge. Dengue fever cases in Situbondo Regency increased from 331 in 2020 to 475 in 2021 with a Case Fatality Rate (CFR) of 0.8%. Delays in diagnosing disease symptoms and lack of accurate clinical observation can be the cause of death in patients infected with dengue virus. Supporting examinations such as leukocyte count can be an accurate indicator of clinical observation. Leukocyte count in Dengue Hemorrhagic Fever patients tends to be abnormal or leukopenia. Purpose: to find out the number of leukocytes in Dengue Hemorrhagic Fever (DHF) patients at Mitra Sehat Situbondo Hospital in 2023. Methods: This study is a quantitative research using a cross-sectional study design with a retrospective approach. The research was carried out from January to April 2023. The samples used were secondary data in the form of leukocyte results taken from medical records of patients diagnosed with Dangue Hemorrhagic Fever (DHF) at Mitra Sehat Situbondo Hospital totaling 81 samples using the cohran formula technique. The data analysis technique in this study uses a descriptive approach Results: number of leukocytes <5000 was 59 respondents (72.7%), 5000-10000 was 22 respondents (27.3%), and no number of leukocytes >10000 was found Conclusions: most respondents had a leukocyte count of <5,000 cells/μL (72.75%).
Detection of Hepatitis C Infection in Voluntary Blood Donors at PMI Sleman Yogyakarta Using Molecular Methods Anggraeni, Dewi Nur
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.23

Abstract

Background: Hepatitis C remains one of the major health problems in the world. Nearly 500,000 deaths are reported each year as a consequence of HCV-related complications. One of the risks of transmission of the hepatitis C virus is through blood transfusions. Enzyme Chemiluminescence Immunoassay is one of the standard methods in blood services to detect HCV in donor blood. However, this method still has limitations in detecting HCV when it is in the window phase of the period. Therefore, molecular techniques such as PCR can be performed to detect HCV genetic material. Purpose: To detect Hepatitis C infection in voluntary blood donors at PMI Sleman Yogyakarta using the molecular method Methods: The design of this study is descriptive research with a cross-sectional study design. This research was conducted from September 2022 to February 2023. This research was carried out at PMI Sleman, Yogyakarta. The sample in this study is voluntary donor blood recorded at PMI Sleman in 2022. The sample has undergone the IMLTD test of the ECHLIA method for 45 people with non-reactive results. The sampling technique of this study uses purposive sampling. The data analysis technique in this study uses a descriptive approach. Blood samples were examined using the Real-Time PCR/qPCR method. Results: Sample No.3 showed the presence of an amplification curve and crossed the threshold line at the value of Ct/Cq = 24.29, but this result showed a negative result. It is because the amplification curve that shows positive HCV appears at Ct 29.46, 29.30, 28.48, 28.22, 31.09, 33.78, and 36.87. Conclusions: All blood samples of non-reactive IMLTD donors using the ECHLIA method showed negative results because the genetic material of HCV was not found using the qPCR method.

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