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Hartalina Mufidah
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Journal of Medical Laboratory in Infectious and Degenerative Diseases
Published by Universitas dr. Soebandi
ISSN : -     EISSN : 30325447     DOI : https://doi.org/10.36858/jmid.v1i2
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Public Health
Journal of Medical Laboratory in Infectious and Degenerative Diseases is a scientific journal with the scope of research conducted by lecturers, practitioners, and students. The purpose of publishing this journal is to accommodate scientific writings that have the substance of research activities, which are the downstream results of research carried out based on an analysis of the needs of the community for scientific and technological contributions in the fields of Parasitology, Bacteriology, Virology, Hematology, Clinical Chemistry, Immunology, Molecular Biology, Cytohistotechnology, Micology, Clinical Toxicology, and Degenerative Diseases.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 5 Documents
 1 
Search results for , issue "Vol. 2 No. 1 (2024): June" : 5 Documents clear
Preferences for breeding places for Aedes spp. mosquitoes on materials and location of containers in the working area of Lateri Health Center, Ambon City Akollo, Robi
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.16

Abstract

Background: The mosquitoes Aedes aegypti and Ae. albopictus is a vector of dengue fever in Indonesia. Container characteristics such as material, color, location, shape, volume and water origin influence female Aedes spp mosquitoes in choosing a place to lay their eggs. Mosquito Ae. aegypti prefers artificial containers as a breeding place, while the Ae. albopictus prefers natural containers. Mosquito Ae. aegypti prefers to be indoors while the Ae. albopictus outdoors. Purpose: knowing the preferences for breeding places for Ae mosquitoes. aegypti and Ae. albopictus on materials and location of containers in the Lateri Health Center Working Area, Ambon City. Methods: This research is a descriptive and analytical study with a cross sectional research design. Larvae collection was carried out in Lateri and Lata villages, Lateri Health Center Working Area. Habitat characterization of Ae. aegypti and Ae. albopictus is carried out visually including the location and material of the container. Identification of larvae using the Aedes genus identification key with a microscope. Results: The container materials found at the research location were artificial containers. The basic materials for the containers made in this research are cement, ceramic, plastic, glass, rubber and aluminum. Mosquito larvae Ae. aegypti are more commonly found inside homes while the mosquito larvae of Ae. albopictus is more commonly found outside the home. Conclusions: Mosquito Ae. aegypti and Ae. albopictus uses artificial containers as a breeding place. Mosquito larvae Ae. aegypti are more commonly found inside homes, while Ae. albopictus outdoors
Optimization of the Formula of Hand Sanitizer Preparation of Teak Leaf Extract (Tectona grandis) as an Antibacterial Isnawati, Nafisah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.18

Abstract

Background: One of the major causes of diarrhea is the bacterium Escherichia coli. This bacterium is commonly found on the palms of hands. To address diarrhea, prevention is necessary, one of which is by using ethanol extract of teak leaves (Tectona grandis). Teak leaves (Tectona grandis) contain secondary metabolites such as flavonoids, saponins, tannins, steroids, and triterpenoids that act as antibacterial agents. Purpose: This study aims to determine that the ethanol extract of teak leaves (Tectona grandis) can be formulated into a hand sanitizer with antibacterial properties Methods: The design of this research study is laboratory experimental, with the process of making extracts, formulating them, evaluating the physical quality of preparations, and performing antibacterial tests. The extract was made using the maceration method with 70% ethanol solvent. Hand sanitizer preparations for teak leaf extract (Tectona grandis) are formulated with three concentrations, namely 10%, 12%, and 15%. Results: The results of the organoleptic evaluation showed a medium to dark brown color, with a semi-solid texture and a characteristic teak leaf aroma. The homogeneity test results showed that all formulations were homogeneous. The pH test results were 7.06 for F1, 7.11 for F2, and 7.12 for F3. The spreadability test results were 5.09 cm for F1, 5.42 cm for F2, and 5.14 cm for F3. The adhesion test results were 10.34 seconds for F1, 9.53 seconds for F2, and 12.71 seconds for F3. The viscosity test results were 2,867 cps for F1, 2,567 cps for F2, and 2,611 cps for F3. The antibacterial activity test showed that the hand sanitizer had antibacterial activity with extract concentrations of 10%, 12%, and 15%. The inhibition zone diameters were 12.67 mm for F1, 15 mm for F2, and 21.67 mm for F3. Conclusions: The ethanol extract of teak leaves (Tectona grandis) can be formulated into an emulgel hand sanitizer with antibacterial properties against Escherichia coli, and all three formulations meet the preparation evaluation requirements. The best formula was at a 15% concentration with an inhibition zone of 21.67 mm.
Profile Of SGPT (Serum Glutamic Pyruvic Transaminase) Levels In Active Smokers Aged 20-60 Years Sudarwo, Dimas Taufiqur Rohman; Sholihatil Hidayati; Ahdiah Imroatul M
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.20

Abstract

Background: Cigarettes are one of the main risk factors for several chronic diseases that can result in death. This shows that cigarettes are a big problem for public health. Purpose: to determine the SGPT (Serum Glutamic Pyruvic Transaminase) level profile in active smokers aged 20-60 years Methods: This research is quantitative. The population in this study is active smoking patients at the Grand Raya Husada Clinic, Pandanarum Village, Lumajang Regency, which totals 60 active smoking patients. The data analysis technique used in this study is descriptive. Results: The results of laboratory examinations showed that serum SGPT (Serum Glutamic Pyruvic Transaminase) levels in 60 respondents showed that 55 respondents (91.67%) had normal SGPT levels. As many as 5 respondents (8.33%) showed high or abnormal SGPT levels. Conclusions: Normal SGPT levels in smokers aged 20-60 years were 91.67%, and high or abnormal SGPT levels were 8.33%.
Analysis of the Number of Leukocytes in Dengue Hemorrhagic Fever Patients at Mitra Sehat Situbondo Hospital in 2023 Oktafiyan, Sheli Tiara
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.22

Abstract

Background: Dengue Hemorrhagic Fever is an infectious disease that still poses a significant public health challenge. Dengue fever cases in Situbondo Regency increased from 331 in 2020 to 475 in 2021 with a Case Fatality Rate (CFR) of 0.8%. Delays in diagnosing disease symptoms and lack of accurate clinical observation can be the cause of death in patients infected with dengue virus. Supporting examinations such as leukocyte count can be an accurate indicator of clinical observation. Leukocyte count in Dengue Hemorrhagic Fever patients tends to be abnormal or leukopenia. Purpose: to find out the number of leukocytes in Dengue Hemorrhagic Fever (DHF) patients at Mitra Sehat Situbondo Hospital in 2023. Methods: This study is a quantitative research using a cross-sectional study design with a retrospective approach. The research was carried out from January to April 2023. The samples used were secondary data in the form of leukocyte results taken from medical records of patients diagnosed with Dangue Hemorrhagic Fever (DHF) at Mitra Sehat Situbondo Hospital totaling 81 samples using the cohran formula technique. The data analysis technique in this study uses a descriptive approach Results: number of leukocytes <5000 was 59 respondents (72.7%), 5000-10000 was 22 respondents (27.3%), and no number of leukocytes >10000 was found Conclusions: most respondents had a leukocyte count of <5,000 cells/μL (72.75%).
Detection of Hepatitis C Infection in Voluntary Blood Donors at PMI Sleman Yogyakarta Using Molecular Methods Anggraeni, Dewi Nur
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 1 (2024): June
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i1.23

Abstract

Background: Hepatitis C remains one of the major health problems in the world. Nearly 500,000 deaths are reported each year as a consequence of HCV-related complications. One of the risks of transmission of the hepatitis C virus is through blood transfusions. Enzyme Chemiluminescence Immunoassay is one of the standard methods in blood services to detect HCV in donor blood. However, this method still has limitations in detecting HCV when it is in the window phase of the period. Therefore, molecular techniques such as PCR can be performed to detect HCV genetic material. Purpose: To detect Hepatitis C infection in voluntary blood donors at PMI Sleman Yogyakarta using the molecular method Methods: The design of this study is descriptive research with a cross-sectional study design. This research was conducted from September 2022 to February 2023. This research was carried out at PMI Sleman, Yogyakarta. The sample in this study is voluntary donor blood recorded at PMI Sleman in 2022. The sample has undergone the IMLTD test of the ECHLIA method for 45 people with non-reactive results. The sampling technique of this study uses purposive sampling. The data analysis technique in this study uses a descriptive approach. Blood samples were examined using the Real-Time PCR/qPCR method. Results: Sample No.3 showed the presence of an amplification curve and crossed the threshold line at the value of Ct/Cq = 24.29, but this result showed a negative result. It is because the amplification curve that shows positive HCV appears at Ct 29.46, 29.30, 28.48, 28.22, 31.09, 33.78, and 36.87. Conclusions: All blood samples of non-reactive IMLTD donors using the ECHLIA method showed negative results because the genetic material of HCV was not found using the qPCR method.

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