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Elza Triani
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cic.jouabe@gmail.com
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Cahaya Ilmu Cendekia Publisher, Jl. Danau No 35 Rt 004 Rw 001. Kel. Dusun Besar, Kec. Singaran Pati, Kota Bengkulu, 38229, Indonesia
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Journal of Academic Biology and Biology Education
ISSN : 30631645     EISSN : 30629705     DOI : https://doi.org/10.37251/jouabe
Focus and scope: Journal of Academic Biology and Biology Education is a double-blind peer-reviewed journal published by Cahaya Ilmu Ilmu Scholar, dedicated to disseminating advances in science and research in the field of biology education both in Indonesia and in the global context in other countries. develop. Committed to excellence, the Journal of Academic Biology and Biology Education publishes comprehensive research articles and invites reviews from leading multidisciplinary educational experts to optimally contribute to policy and practice. The selection criteria prioritize papers that demonstrate high scientific value, convey new knowledge, and have a significant impact on biology education. The focus of this journal is evaluation, ethnobiology, biology teaching, PISA Tasks, and the learning of biology and related topics at the school and college level.
Arjuna Subject : Umum - Umum
Articles 101 Documents
The Activity of Papaya Seeds (Carica papaya L.) Varieties of “Bangkok” and “California” in Inhibiting the Growth of Pathogenic Bacteria Roszelina Abd Rahman; Hilda Awaliah; Nora Syazehan Jems
Journal of Academic Biology and Biology Education Vol. 2 No. 1 (2025): June
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/jouabe.v2i1.2190

Abstract

Purpose of the study: The purpose of this study is to evaluate the antibacterial activity of ethanol extracts from Bangkok and California papaya (Carica papaya) seeds against E. coli, Salmonella sp., and S. aureus, and to identify the chemical compounds responsible for their antibacterial properties using GC–MS analysis. Methodology: This study applied a Completely Randomized Design (CRD) using ethanol extracts of Carica papaya seeds. Equipment included GC–MS, rotary evaporator, autoclave, laminar flow, vortex mixer, hot plate, incubator, and micrometer. Media and reagents were NA, NB, MHA, ethanol, DMSO, and Amoxicillin. Tested bacteria were E. coli, Salmonella sp., and S. aureus. Data were analyzed with ANOVA and DMRT using SPSS. Main Findings: Ethanol extracts of Carica papaya seeds (‘Bangkok’ and ‘California’) significantly inhibited E. coli, Salmonella sp., and S. aureus growth. The highest inhibition zones occurred at 20% concentration, with ‘California’ showing stronger activity against Salmonella sp. and ‘Bangkok’ against S. aureus. MIC was 20% for Gram-negative bacteria. GC–MS identified 20 compounds in ‘Bangkok’ and 24 in ‘California’, dominated by hexadecanoic acid and various alkaloids, flavonoids, terpenoids, and fatty acids. Novelty/Originality of this study: This study provides new insights by comparing the antibacterial activity and chemical composition of ethanol extracts from Carica papaya seeds of ‘Bangkok’ and ‘California’ varieties. It identifies specific bioactive compounds through GC–MS and reveals variety-dependent antibacterial effects, advancing knowledge of papaya seeds as natural antibacterial agents and supporting their potential development as alternative antimicrobial resources.

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