cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Juaini Pratama
Contact Email
inbio.publine@gmail.com
Phone
+6281917138845
Journal Mail Official
inbio.publine@gmail.com
Editorial Address
Jl. Tawak-tawak No.5 Karang Sukun, Kel. Mataram Timur, Kec. Mataram, Kota Mataram - NTB, Indonesia 83121
Location
Kota mataram,
Nusa tenggara barat
INDONESIA
Insight of Biology
Published by Lembaga Publikasi Ilmiah Nusantara
ISSN : -     EISSN : 31091164     DOI : https://doi.org/10.70716/inbio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
nsight of Biology (INBIO) is a scientific journal in biology that contains research results and literature studies. It managed and published by Lembaga Publikasi Ilmiah Nusantara. INBIO journal provides a platform for researchers, academics, professionals, practitioners and students to embed and share knowledge in the form of empirical and theoretical research papers, case studies, literature reviews and book reviews related to biology science. We will process all receipts of the script in a double-anonymized review by Bestari partners.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 20 Documents
 1   2 
Perubahan Sitokin Pro-inflamasi setelah Pemberian Jahe Merah pada Mencit dengan Paparan Asap Rokok: Studi In Vivo Putra, Aditya; Pratama, Farhan
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.342

Abstract

Exposure to cigarette smoke is known to trigger oxidative stress and inflammatory responses, characterized by increased levels of pro-inflammatory cytokines such as Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-6 (IL-6). Excessive inflammatory responses can disrupt cellular homeostasis and contribute to tissue damage. Red ginger contains bioactive compounds, including gingerols and shogaols, which exhibit strong antioxidant and anti-inflammatory activities. This study aims to analyze changes in TNF-α and IL-6 levels in mice following the administration of red ginger extract under cigarette smoke exposure. An in vivo design was used by dividing mice into several groups: a control group, a group exposed to cigarette smoke, and a group exposed to cigarette smoke with red ginger extract treatment. After the treatment period, TNF-α and IL-6 levels were measured through serum analysis using immunological methods. The results showed that mice receiving red ginger extract experienced a significant reduction in TNF-α and IL-6 levels compared to the smoke-exposed group without treatment. These findings indicate that red ginger has potential protective effects by modulating inflammatory responses induced by cigarette smoke exposure. Therefore, red ginger can be considered a natural anti-inflammatory agent for reducing the negative impact of oxidative stress from cigarette smoke.
Pemanfaatan fraksi aktif ekstrak kecambah Vigna radiata L. sebagai bioregulator pada kultur in vitro planlet Nepenthes sp. menggunakan medium Murashige & Skoog Puspita, Dewi; Santoso, Maya
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.343

Abstract

This study aimed to evaluate the potential of the active fraction from Vigna radiata L. sprout extract as a natural bioregulator in the in vitro culture of Nepenthes sp. plantlets using Murashige & Skoog (MS) medium. The use of natural plant growth regulators offers an alternative to reduce dependence on synthetic growth regulators, particularly for carnivorous plants that generally exhibit slow growth rates and sensitive responses in tissue culture. The active fraction was obtained through stepwise fractionation to isolate bioactive compounds that may stimulate plant growth. Plantlets were cultured on MS medium supplemented with various concentrations of the active fraction to observe growth responses, including shoot elongation, leaf number, root development, and overall biomass accumulation. The results are expected to identify the optimum concentration that provides the highest growth stimulation, demonstrating that Vigna radiata sprout extract can serve as an efficient, economical, and environmentally friendly natural bioregulator. These findings contribute to the development of improved propagation protocols for Nepenthes sp. and highlight the potential of sprout-derived natural compounds for broader applications in plant tissue culture.
Optimasi Rasio Ekstrak Bahan Organik Alami dalam Formulasi Media Kultur untuk Regenerasi In Vitro Aglonema Varietas Red Borju Permata, Citra; Kartika, Ikhsan
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.345

Abstract

This study aimed to optimize the ratio of natural organic extracts derived from shallot (Allium cepa) and mung bean sprouts (Phaseolus radiatus) in culture media formulations to enhance in vitro regeneration of Aglonema cv. Red Borju. These organic materials are known to contain natural phytohormones, such as auxins and cytokinins, as well as other bioactive compounds that potentially stimulate morphogenesis. The experiment was conducted using several extract ratio treatments incorporated into Murashige and Skoog (MS) basal medium. Growth responses were evaluated based on plantlet height, number of leaves, number of roots, fresh weight, and dry weight. The results demonstrated that variations in extract ratios significantly affected plantlet growth performance. The combination of shallot and mung bean sprout extracts at a ratio of 2:1 produced the most significant improvement in all observed growth parameters. The use of natural organic extracts proved to be an effective alternative to partially replace synthetic components in culture media without reducing growth efficiency. This study contributes to the development of environmentally friendly and cost-effective in vitro culture techniques for the mass propagation of Aglonema cv. Red Borju.
Model Literasi Ekologi Integratif Berbasis Komunitas untuk Mahasiswa Calon Guru Biologi: Desain, Implementasi, dan Evaluasi Sari, Rangga; Saputra, Andi
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.346

Abstract

Strengthening ecological literacy is an urgent necessity in pre-service biology teacher education, given the increasing complexity of environmental challenges and the demands of 21st-century learning. This study aims to develop and evaluate an Integrative Community-Based Ecological Literacy Model designed to enhance students’ conceptual understanding, critical thinking skills, and ecological awareness. The model integrates project-based learning, field experiences, and active collaboration with local communities as authentic learning resources. A mixed-methods research design was employed, consisting of needs analysis, model development, implementation, and evaluation stages. Data were collected through ecological literacy tests, observations, interviews, and analysis of students’ project outputs. The findings indicate that the model effectively improves students’ holistic understanding of ecological concepts, strengthens their analytical abilities in addressing environmental issues, and fosters active participation in community-based conservation activities. These results highlight that integrating community contexts into ecological learning enriches the learning process and optimizes the role of pre-service biology teachers as agents of environmental change. The developed model is recommended for adaptation in various educational contexts that emphasize environmental sustainability.
Peran Stimulus Lingkungan terhadap Mekanisme Biologi Pembelajaran : Kajian Neurofisiologis pada Peserta Didik Hidayat, Doni; Sartika, Fikri
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.347

Abstract

The validation results of the project-based learning model integrated with green economy concepts indicate that the developed model falls into the highly valid category based on expert evaluations. The validation process involved three experts from relevant fields who assessed five main aspects: model construction, clarity of learning syntax, content suitability, integration of green economy principles, and the model’s potential to enhance sustainability literacy. The overall mean score obtained was 3.62 out of a maximum score of 4.00, demonstrating a very high level of validity. Quantitative findings were reinforced by qualitative feedback from experts, who highlighted the systematic structure of the learning stages, particularly in the problem exploration and project design phases. The clarity of the project-based learning syntax was rated very highly, especially in the phase that encourages students to investigate and analyze real-world green economy issues. Although the integration of green economy concepts was considered appropriate, experts suggested strengthening the use of empirical data and balancing economic, social, and environmental sustainability indicators. Overall, the validation results suggest that the proposed learning model is theoretically sound and feasible for implementation in economics education. This model has strong potential to improve students’ sustainability literacy by fostering knowledge, skills, and attitudes aligned with sustainable development principles and can serve as a foundation for further studies on practicality and effectiveness.
Analisis Biologi Terapan Berbasis Variabel Lingkungan dan Molekuler Melati Permata; Bayu Rahman
Insight of Biology Vol. 2 No. 1 (2026): Insight of Biology, March 2026
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v2i1.437

Abstract

The advancement of applied biology based on molecular approaches has significantly improved the accuracy of environmental analysis. This study aims to examine the relationship between environmental variables and molecular responses of organisms as indicators of ecosystem change. The research applies an integrative approach using environmental DNA (eDNA), environmental RNA (eRNA), molecular biomarkers, and mass spectrometry techniques for environmental monitoring. Data were compiled from recent empirical studies demonstrating that eDNA-based methods achieve species-level detection accuracy exceeding 90%. In addition, molecular biomarkers enable the identification of pollutant exposure at the sub-cellular level prior to observable ecological impacts. The results indicate that environmental variables such as temperature, pH, and pollutant concentration show strong correlations with gene expression and microbial community dynamics. The integration of molecular and environmental data produces more accurate predictive models for detecting ecosystem degradation. This study confirms that molecular biology approaches play a critical role in developing precise, rapid, and sustainable biomonitoring systems for environmental management.
Pengaruh Konsentrasi Mikroplastik terhadap Aktivitas Enzim Antioksidan dan Pertumbuhan Chlorella vulgaris dalam Sistem Kultur Terkontrol Dewi Kartika Sari; Ahmad Rahman
Insight of Biology Vol. 2 No. 1 (2026): Insight of Biology, March 2026
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v2i1.438

Abstract

The increasing accumulation of microplastics in aquatic environments poses a significant threat to photosynthetic organisms, including the microalga Chlorella vulgaris. This study aims to analyze the effects of varying microplastic concentrations on antioxidant enzyme activity and growth of C. vulgaris under controlled culture conditions. The experiment applied environmentally relevant and sub-lethal concentrations of microplastics. Observed parameters included growth rate, chlorophyll content, and antioxidant enzyme activities such as superoxide dismutase (SOD) and catalase (CAT). The results show that increasing microplastic concentrations reduced growth rate by more than 35% at higher exposure levels and significantly elevated antioxidant enzyme activity as a response to oxidative stress. SOD and CAT activities increased by approximately 1.8–2.3 times compared to the control, indicating the accumulation of reactive oxygen species. In addition, microplastics disrupted photosynthetic processes and cellular energy metabolism. These findings demonstrate that microplastics act as environmental stressors that trigger physiological and biochemical responses in C. vulgaris. This study highlights the importance of assessing microplastic risks to primary producers in aquatic ecosystems and their implications for food web stability.
Analisis Hubungan Keanekaragaman Mikroba Tanah dan Produktivitas Tanaman Padi pada Sistem Pertanian Organik dan Konvensional Budi Santoso; Siti Rahmawati
Insight of Biology Vol. 2 No. 1 (2026): Insight of Biology, March 2026
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v2i1.439

Abstract

Soil microbial diversity plays a critical role in determining rice productivity through nutrient cycling, decomposition processes, and rhizosphere interactions. This study aims to analyze the relationship between soil microbial diversity and rice productivity under organic and conventional farming systems. A comparative approach was applied using empirical data from long-term studies that evaluated microbial communities and crop yield performance. The results indicate that organic systems exhibit higher microbial diversity, with a 15–35% increase in the Shannon diversity index compared to conventional systems. Functional microbial activities, including phosphate-solubilizing and nitrogen-fixing bacteria, were also higher under organic management. Rice productivity in organic systems showed greater long-term stability, although initial yields tended to be lower than those in conventional systems. Correlation analysis revealed a significant positive relationship between microbial diversity and crop yield (r = 0.72; p < 0.01). These findings highlight that soil microbiome management is a key factor in enhancing productivity and sustainability in rice farming systems.
Peran Ekspresi Gen Heat Shock Protein (HSP70) terhadap Respon Fisiologis Ikan Nila (Oreochromis niloticus) pada Stres Suhu Tinggi Rina Wulandari; Andi Prakoso
Insight of Biology Vol. 2 No. 1 (2026): Insight of Biology, March 2026
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v2i1.441

Abstract

Elevated water temperature is a major stressor in aquaculture systems that disrupts the physiological stability of Nile tilapia (Oreochromis niloticus). This study aims to analyze the role of heat shock protein 70 (HSP70) gene expression in response to high temperature stress and its association with physiological changes in fish. The study applies a synthesis of empirical data from experimental research that measures molecular, biochemical, and physiological responses to elevated temperature exposure. The results show that increased temperature significantly induces HSP70 gene expression as a protective mechanism against protein denaturation and cellular damage. Elevated HSP70 expression correlates with stress indicators, including increased cortisol levels, enhanced antioxidant enzyme activity, and metabolic imbalance. Under higher stress intensity, this protective response fails to fully compensate for cellular damage, as indicated by reduced physiological performance and increased tissue damage. These findings confirm that HSP70 functions as a sensitive biomarker for detecting thermal stress and plays a critical role in the adaptive mechanisms of fish under environmental change.
Pengaruh Paparan Mikroplastik terhadap Stres Oksidatif dan Kinerja Pertumbuhan pada Ikan Nila (Oreochromis niloticus) Andi Sudradjat; Rizky Pramono
Insight of Biology Vol. 2 No. 1 (2026): Insight of Biology, March 2026
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v2i1.446

Abstract

Microplastic pollution in aquatic environments has increased significantly and poses a threat to the health of cultured fish, including Nile tilapia (Oreochromis niloticus). This study aims to analyze the effects of microplastic exposure on oxidative stress and growth performance in Nile tilapia. The study applies an experimental approach based on the synthesis of empirical data from recent studies that evaluate physiological, biochemical, and growth responses of fish to microplastic exposure. The results indicate that microplastics induce the production of reactive oxygen species, leading to the activation of antioxidant defense systems and an increase in oxidative stress indicators. This condition is followed by a significant decline in specific growth rate, feed utilization efficiency, and fish survival rate. Long-term exposure also contributes to tissue damage and impaired organ function. These findings confirm that microplastics act as an environmental stressor that directly disrupts physiological balance and production performance in Nile tilapia. This study provides a scientific basis for more adaptive aquaculture environmental management in response to microplastic contamination risks.

Page 2 of 2 | Total Record : 20

 1   2 

Filter by Year

2025 2026


Reset
Filter By Issues
All Issue Vol. 2 No. 1 (2026): Insight of Biology, March 2026 Vol. 1 No. 3 (2025): Insight of Biology, December 2025 Vol. 1 No. 2 (2025): Insight of Biology, September 2025 Vol. 1 No. 1 (2025): Insight of Biology, June 2025