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Contact Name
Juaini Pratama
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INDONESIA
Insight of Biology
ISSN : -     EISSN : 31091164     DOI : https://doi.org/10.70716/inbio
nsight of Biology (INBIO) is a scientific journal in biology that contains research results and literature studies. It managed and published by Lembaga Publikasi Ilmiah Nusantara. INBIO journal provides a platform for researchers, academics, professionals, practitioners and students to embed and share knowledge in the form of empirical and theoretical research papers, case studies, literature reviews and book reviews related to biology science. We will process all receipts of the script in a double-anonymized review by Bestari partners.
Articles 15 Documents
Perubahan Sitokin Pro-inflamasi setelah Pemberian Jahe Merah pada Mencit dengan Paparan Asap Rokok: Studi In Vivo Putra, Aditya; Pratama, Farhan
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.342

Abstract

Exposure to cigarette smoke is known to trigger oxidative stress and inflammatory responses, characterized by increased levels of pro-inflammatory cytokines such as Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-6 (IL-6). Excessive inflammatory responses can disrupt cellular homeostasis and contribute to tissue damage. Red ginger contains bioactive compounds, including gingerols and shogaols, which exhibit strong antioxidant and anti-inflammatory activities. This study aims to analyze changes in TNF-α and IL-6 levels in mice following the administration of red ginger extract under cigarette smoke exposure. An in vivo design was used by dividing mice into several groups: a control group, a group exposed to cigarette smoke, and a group exposed to cigarette smoke with red ginger extract treatment. After the treatment period, TNF-α and IL-6 levels were measured through serum analysis using immunological methods. The results showed that mice receiving red ginger extract experienced a significant reduction in TNF-α and IL-6 levels compared to the smoke-exposed group without treatment. These findings indicate that red ginger has potential protective effects by modulating inflammatory responses induced by cigarette smoke exposure. Therefore, red ginger can be considered a natural anti-inflammatory agent for reducing the negative impact of oxidative stress from cigarette smoke.
Pemanfaatan fraksi aktif ekstrak kecambah Vigna radiata L. sebagai bioregulator pada kultur in vitro planlet Nepenthes sp. menggunakan medium Murashige & Skoog Puspita, Dewi; Santoso, Maya
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.343

Abstract

This study aimed to evaluate the potential of the active fraction from Vigna radiata L. sprout extract as a natural bioregulator in the in vitro culture of Nepenthes sp. plantlets using Murashige & Skoog (MS) medium. The use of natural plant growth regulators offers an alternative to reduce dependence on synthetic growth regulators, particularly for carnivorous plants that generally exhibit slow growth rates and sensitive responses in tissue culture. The active fraction was obtained through stepwise fractionation to isolate bioactive compounds that may stimulate plant growth. Plantlets were cultured on MS medium supplemented with various concentrations of the active fraction to observe growth responses, including shoot elongation, leaf number, root development, and overall biomass accumulation. The results are expected to identify the optimum concentration that provides the highest growth stimulation, demonstrating that Vigna radiata sprout extract can serve as an efficient, economical, and environmentally friendly natural bioregulator. These findings contribute to the development of improved propagation protocols for Nepenthes sp. and highlight the potential of sprout-derived natural compounds for broader applications in plant tissue culture.
Optimasi Rasio Ekstrak Bahan Organik Alami dalam Formulasi Media Kultur untuk Regenerasi In Vitro Aglonema Varietas Red Borju Permata, Citra; Kartika, Ikhsan
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.345

Abstract

This study aimed to optimize the ratio of natural organic extracts derived from shallot (Allium cepa) and mung bean sprouts (Phaseolus radiatus) in culture media formulations to enhance in vitro regeneration of Aglonema cv. Red Borju. These organic materials are known to contain natural phytohormones, such as auxins and cytokinins, as well as other bioactive compounds that potentially stimulate morphogenesis. The experiment was conducted using several extract ratio treatments incorporated into Murashige and Skoog (MS) basal medium. Growth responses were evaluated based on plantlet height, number of leaves, number of roots, fresh weight, and dry weight. The results demonstrated that variations in extract ratios significantly affected plantlet growth performance. The combination of shallot and mung bean sprout extracts at a ratio of 2:1 produced the most significant improvement in all observed growth parameters. The use of natural organic extracts proved to be an effective alternative to partially replace synthetic components in culture media without reducing growth efficiency. This study contributes to the development of environmentally friendly and cost-effective in vitro culture techniques for the mass propagation of Aglonema cv. Red Borju.
Model Literasi Ekologi Integratif Berbasis Komunitas untuk Mahasiswa Calon Guru Biologi: Desain, Implementasi, dan Evaluasi Sari, Rangga; Saputra, Andi
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.346

Abstract

Strengthening ecological literacy is an urgent necessity in pre-service biology teacher education, given the increasing complexity of environmental challenges and the demands of 21st-century learning. This study aims to develop and evaluate an Integrative Community-Based Ecological Literacy Model designed to enhance students’ conceptual understanding, critical thinking skills, and ecological awareness. The model integrates project-based learning, field experiences, and active collaboration with local communities as authentic learning resources. A mixed-methods research design was employed, consisting of needs analysis, model development, implementation, and evaluation stages. Data were collected through ecological literacy tests, observations, interviews, and analysis of students’ project outputs. The findings indicate that the model effectively improves students’ holistic understanding of ecological concepts, strengthens their analytical abilities in addressing environmental issues, and fosters active participation in community-based conservation activities. These results highlight that integrating community contexts into ecological learning enriches the learning process and optimizes the role of pre-service biology teachers as agents of environmental change. The developed model is recommended for adaptation in various educational contexts that emphasize environmental sustainability.
Peran Stimulus Lingkungan terhadap Mekanisme Biologi Pembelajaran : Kajian Neurofisiologis pada Peserta Didik Hidayat, Doni; Sartika, Fikri
Insight of Biology Vol. 1 No. 3 (2025): Insight of Biology, December 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/inbio.v1i3.347

Abstract

The validation results of the project-based learning model integrated with green economy concepts indicate that the developed model falls into the highly valid category based on expert evaluations. The validation process involved three experts from relevant fields who assessed five main aspects: model construction, clarity of learning syntax, content suitability, integration of green economy principles, and the model’s potential to enhance sustainability literacy. The overall mean score obtained was 3.62 out of a maximum score of 4.00, demonstrating a very high level of validity. Quantitative findings were reinforced by qualitative feedback from experts, who highlighted the systematic structure of the learning stages, particularly in the problem exploration and project design phases. The clarity of the project-based learning syntax was rated very highly, especially in the phase that encourages students to investigate and analyze real-world green economy issues. Although the integration of green economy concepts was considered appropriate, experts suggested strengthening the use of empirical data and balancing economic, social, and environmental sustainability indicators. Overall, the validation results suggest that the proposed learning model is theoretically sound and feasible for implementation in economics education. This model has strong potential to improve students’ sustainability literacy by fostering knowledge, skills, and attitudes aligned with sustainable development principles and can serve as a foundation for further studies on practicality and effectiveness.

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