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Articles 5 Documents
 1 
Search results for , issue "Vol. 12 No. 1 (2025)" : 5 Documents clear
The Antagonistic Potential of Kimchi-Associated LAB Against Histamine-Producing Pathogens in Seafood Putu Satrio Jhanardana Dewantara; Ni Putu Bintang Diah Lastini; Made Okta Hapsari Putri Diarsa; Pande Gede Putra Satwika; Dwi Ariani Yuliastuti; I Dewa Agung Panji Dwipayana; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i01.p3

Abstract

Lactic acid bacteria (LAB) play a crucial role in food fermentation, for example in kimchi, a traditional Korean dish. These bacteria don’t only function in food preservation and probiotics, but also exhibit antimicrobial activity through the production of compounds like bacteriocins. This study introduces a novel approach in using LAB from kimchi for inhibiting the growth of Morganella morganii, a major histamine-producing pathogen in fish products. Histamine accumulation poses severe food safety risks, necessitating an innovative natural solutions. LAB isolates from both solid and liquid fractions of kimchi were screened on selective media and characterized biochemically for traits such as fermentation type and acid production. Antimicrobial efficacy, assessed via the Kirby-Bauer disk diffusion method, revealed inhibition zones averaging 6.98 ± 0.04 mm for liquid and 6.40 ± 1.34 mm for solid fractions. Despite their lower efficacy compared to chloramphenicol (30.80 ± 1.92 mm), the eco-friendly nature of LAB highlights their unique potential as a sustainable alternative for histamine control. This study is among the first to explore kimchi-derived LAB as antagonists against M. morganii, providing a novel perspective for developing green technologies in food safety and quality management.
Formulasi Sediaan Lotion Antinyamuk Dari Ekstrak Daun Malapari (Pongamia Pinnata (L.) Pierre) Terhadap Nyamuk Aedes Aegypti (Linnaeus, 1762) I Gusti Ayu Suasthiti Asri; Ni Luh Arpiwi; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i01.p2

Abstract

Nyamuk merupakan vektor penyakit yang dapat menyebarkan beberapa jenis penyakit seperti Demam Berdarah Dengue (DBD), Malaria, Filariasis, Chikungunya dan Japanese encephalitis. Masyarakat diharapkan untuk selalu meningkatkan kewaspadaan terhadap keberadaan nyamuk khususnya pada nyamuk Aedes aegypti yang dapat membawa virus dengue penyebab DBD. Salah satu cara untuk memberikan perlindungan kulit dari gigitan nyamuk dan dapat diterapkan sehari-hari secara terus-menerus yaitu dengan mengaplikasikan bahan antinyamuk kedalam lotion yang terbuat dari ekstrak daun malapari yang memiliki beberapa senyawa kandungan tumbuhan obat yang berkhasiat sebagai antinyamuk diantaranya: flavonoid, steroid, alkaloid dan terpenoid. Pembuatan ekstrak daun malapari pada lotion ini menggunakan metode ekstraksi dengan cara maserasi. Adapun tujuan dari pembuatan lotion antinyamuk yaitu untuk mengetahui daya proteksi lotion antinyamuk dengan konsentrasi yang berbeda terhadap gigitan nyamuk Ae. aegypti dan mengetahui tingkat kesukaan probandus terhadap lotion antinyamuk ekstrak daun malapari. Hasil uji daya proteksi menunjukkan persentase daya proteksi berbeda nyata antar perlakuannya (P<0,05). Formulasi lotion F3 memiliki daya proteksi tertinggi hingga jam ke-5, kemudian lotion F2 dengan daya proteksi hingga jam ke-2, lalu lotion F1 dengan daya proteksi hanya di jam ke-1, dan lotion F0 yang sama sekali tidak memiliki daya proteksi dari jam ke-1 hingga jam ke-6. Tingkat kesukaan probandus terhadap formulasi lotion F1 mendapat penilaian sangat disukai, kemudian lotion F0 mendapat penilaian suka, lotion F2 dengan penilaian tidak suka dan lotion F3 mendapat penilaian sangat tidak disukai oleh probandus. Kata kunci: antinyamuk, Aedes aegypti, lotion, malapari
The Content of Lead Metals in Water and Green Shells (Perna viridis) in the Waters of Tanjung Emas Semarang Rizkita Dimar Agnevia; Ni Luh Watiniasih; Alfi Herawati Waskita Sari
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843./metamorfosa./2025.v12.i01.p5

Abstract

Tanjung Emas waters are a coastal area in Semarang City which is a productive area and has potential resources for the country's development in the economic field. The existence of industrial activities and ports in the waters of Tanjung Emas Semarang is suspected to be a contributor to the influx of heavy metals such as Lead (Pb) into the waters. Changes in the polluted water environment will affect the survival of conch animals because these animals have limited mobility and are easily affected by the presence of pollutants, both physical and organic pollutants. The waters of the Port of Tanjung Emas Semarang functioned as a place for fish farming and cultivation of clam. One of them is green shells (Perna viridis). Shellfish belong to the type of filter animal (filter feeder). Water samples and extracted green shells (Perna viridis) were measured for lead content (Pb) using the AAS (Atomic Absorption Spectrophotometer) tool which refers to SNI 6989.4.2009 for water and SNI 01.2896.1998 for green shells (Perna viridis). The results of this study found that the concentration of heavy metals in shells was higher than in water. The measurement results of heavy metals lead (Pb) in water ranged from 0.008 to 0.010 Mg / L while the values ​​in green shells (Perna viridis) ranged from 0.274 to 0.320 Mg / Kg Then for water quality parameters the pH value ranges from 7 to 7.7 while the temperature value is 28.6 - 29.8o C and the value of dissolved oxygen (DO) is 5 - 5.2 ppm. Keywords: Heavy Metal, Pb, Water, Green Shells
Profil Pencemaran Mikroplastik Pada Sedimen Ekosistem Mangrove di Pulau Serangan, Bali Made Bayu Khrisna Pramana; Nyoman Dati Pertami; Ni Luh Gede Rai Ayu Saraswati
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i01.p1

Abstract

Microplastics are defined as small particles plastic with less than 5 mm in size. These small plastic particles were polluted all environments compartments, including mangrove ecosystem. This study aims to investigate microplastics pollution in mangrove sediment in Serangan waters, Bali. Moreover, sediment sample were collected in different sediment depth (0-10 cm, 10-20 cm and 20-30 cm) to understand the vertical abundance and morphology of microplastics in mangrove sediment. Sampling points were also distributed across mangrove ecosystem of Serangan Island with different characteristics, including near road and landfill station (St 1), residential area and small bussineses (St 2 and 3), and near bay area with less anthropogenic influence (St 4). A total of 182 suspected microplastic particles were identified from all sediment sample. The average of microplastics abundance vertically was ranging from 0.025±0.015 to 0.026±0.014 particle/g, while the average abundance horizontally was from 0.021±0.010 to 0.036±0.015 particle/g. Fragment was the most common microplastics types identified (76.37%), followed by fiber (15.38%) and film (8.24%). Our result highlight that microplastics has polluted the sediment layers of mangrove ecosystem in Serangan oWaters. The source of suspected microplastics particle in the sampling area was suggested from both land (through river pathway) and ocean (due to oceanographic factors, such as tides, wind and current). A further study on determining the polymer of suspected microplastics particle is needed. This is important to confirm the validity of visual identification in this study. Keyword: Microplastics, Mangrove ecosystem, Mangrove sediment, Serangan Island
Etnofarmakologi Etnis Madura: Kajian Obat Herbal Tradisional untuk Pengobatan Gejala Tuberkulosis Sonny Kristianto; Rury Eryna Putri; Wimbuh Tri Widodo
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843./metamorfosa./2025.v12.i01.p4

Abstract

Tuberkulosis (TBC) merupakan salah satu penyakit menular yang disebabkan oleh infeksi bakteri Mycobacterium tuberculosis (MTB) dan menyebar melalui udara. Selain relatif mahal dan akses terbatas, fenomena resistensi TBC terhadap obat menjadi hal umum di negara berkembang termasuk Indonesia. Di sisi lain, obat herbal tradisional Indonesia yang dikenal sebagai jamu telah digunakan sejak lama untuk mengobati gejala penyakit TBC. Suku Madura merupakan salah satu suku di Indonesia yang masih menggunakan tanaman herbal sebagai jamu tradisional. Penelitian ini mengeksplor jenis tanaman obat yang digunakan oleh etnis Madura untuk meramu jamu dan menduga kandungan senyawa aktif dalam jamu yang terlibat dalam pengobatan TBC. Pendekatan deskriptif-kualitatif berupa wawancara mendalam pada masyarakat lokal dilakukan untuk menemukan potensi obat dari etnis Madura serta prediksi aktivitas senyawa aktif untuk penanganan gejala penyakit kronis populer berupa TBC. Hasil penelitian menunjukkan bahwa cara pemanfaatan tanaman obat sebagai jamu tradisional oleh etnis Madura masih mempertahankan budaya warisan leluhur dengan menggunakan peralatan tradisional yang terbuat dari tanah. Selain itu, sebagian besar tanaman obat yang dimanfaatkan oleh etnis Madura tergolong dalam famili Zingiberaceae yang memiliki beragam senyawa aktif dengan aktivitas antioksidan, antimikrob, antiinflamasi bahkan antitusif yang baik untuk mengurangi gejala TBC berupa demam dan batuk. Temuan etnofarmakologi ini menjembatani pengetahuan herbal lokal etnis Madura dengan kajian ilmiah modern, serta menunjukkan potensi jamu tradisional sebagai sumber kandidat terapi pendukung untuk penanganan gejala Tuberkulosis (TBC). Kata kunci: antimikroba, etnis Madura, jamu, senyawa aktif, TBC

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