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Esti Tyastirin
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INDONESIA
BIOTROPIC
Published by Universitas Islam Negeri Sunan Ampel
ISSN : -     EISSN : 25805029     DOI : https://doi.org/10.29080/biotropic.v8i2
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropic : The Journal of Tropical Biology publish articles on South East Asia Tropical Biology from various perspectives, covering both literary and fieldwork studies. The Journal put empahis on aspects related to: Ecology Zoology Botany Microbiology Biology molecular Biospeleology Conservation and other tropical biology discourses Journals are published biannually in February and August. Editor invite all relevant parties to contribute in publishing sciencetific articles which have never been published
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 120 Documents
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Molecular Docking Senyawa Heparan Sulfate dan Hyaluronic Acid Lendir Bekicot (Lissachatina fulica) terhadap Reseptor TGF-β pada Proses Penyembuhan Luka Kuni Afifah; Latifah Nurul Aulia; Rochmatul Fitri; Erlix R Purnama
Biotropic : The Journal of Tropical Biology Vol. 8 No. 2 (2024): Biotropic, Volume 8 Nomor 2, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i2.2077

Abstract

Land snails, commonly known as snails (Lissachatina fulica), produce slime or mucus containing Heparan sulfate and Hyaluronic acid compounds which can speed up the wound healing process by helping the blood clotting process and fibroblast proliferation. This study aims to determine the effectiveness of Heparan sulfate and Hyaluronic acid compounds in snail (Lissachatina fulica) mucus which have the potential to help the wound healing process using the TGF-β receptor and the comparison drug Bioplacenton. This research is an observational study using a docking method between the target protein, namely the TGF-β receptor, and the compounds Heparan sulfate, Hyaluronic acid and Neomycin sulfate. The research stages carried out included target prediction, preparation and 3-dimensional structure of the TGF-β protein, preparation and minimization of compound ligands, docking of test ligands against the TGF-β receptor, visualization and analysis of in silico test data in a qualitative descriptive manner. The results of this research show that the Heparan sulfate compound in Snail Mucus (Lissachatina fulica) is more effective as a wound healing medicine compared to hyaluronic acid and can be used as an alternative wound medicine to replace Neomycin sulfate (Bioplacenton medicine).
Kajian Farmakoinformatika Senyawa Caulerchlorin dan Racemosin sebagai kandidat Antimalaria Dewi Ratih Tirto Sari; Nadia Humairoh; Intani Quarta Lailaty; Gabriella Chandrakirana Krisnamurti; Siti Zamilatul Azkiyah
Biotropic : The Journal of Tropical Biology Vol. 8 No. 2 (2024): Biotropic, Volume 8 Nomor 2, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i2.2080

Abstract

Caulerchlorin and racemosin were bioactive compounds containing in Caulerpa racemosa macroalgae. Previous study, those compounds promoted therapeutical target on metabolic syndrome. However, the therapeutical activity on Malaria not yet defined. Therefore, this study investigated potential activity of caulerchlorin and racemosin from Caulerpa racemosa as antimalarial activity through pharmacoinformatic study. Caulerchlorin and racemosin structures were retrieved from PubChem NCBI and the Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH) structure was taken out from Protein database with ID 5GJG. Orotic acid was used as native ligand or control. Ligands were predicted their antiplasmodial activity and toxicity. Ligands and protein were interacted by redocking using Molegro Virtual Docker version 6.0 and analyzed by using PyMol 2.3 and Discovery Studio version 21.1.1. Two bioactive compounds also showed antiplasmodial activity and were not mutagenicity and hepatotoxicity affections. Molecular docking performed that caulerchlorin and racemosin exhibited same active residues as well as orotic acid as native substrate. Binding energy revealed two compounds, caulerchlorin and racemosin showed lower binding energy than orotic acid. In summary caulerchlorin and racemosin were potentially inhibited PfDHODH activity by binding substrate sites of PfDHODH protein.
Pemanfaatan Limbah Pucuk Tebu sebagai Sumber Selulosa Bahan Baku Plastik Biodegradable Eva Agustina; Risa Purnamasari; Nasrul Fuad Erfansyah; Funsu Andiarna; Nova Lusiana; Irul Hidayati
Biotropic : The Journal of Tropical Biology Vol. 8 No. 1 (2024): Biotropic, Volume 8 Nomor 1, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i1.2082

Abstract

Plastic waste continues to be a significant environmental issue due to the extended degradation period it requires. Bioplastics represent an innovation in the conventional plastic cycle, being made from natural materials such as agricultural waste containing cellulose. The objective of this research is to determine the influence of adding cellulose from sugarcane tops in the production of biodegradable plastic. The research method begins with the extraction of cellulose from sugarcane tops, including preparation stages, wax removal, bleaching, hemicellulose removal, and delignification. Sugarcane top cellulose is utilized as a raw material for bioplastic. Cellulose with concentrations of 0%, 10%, 30%, and 50% by weight is processed together with a mixture of water and tapioca, to which chitosan and glycerol are subsequently added. Observation parameters for the bioplastic include functional group characterization and mechanical tests, encompassing transparency, tensile strength, elongation, density, thickness, water absorption, and biodegradability. The research results indicate that the characterization of functional groups using FTIR produces spectra of O-H, C-H, C=C, C-O, indicating the constituent components of cellulose, glycerol, and chitosan. Meanwhile, based on the mechanical tests, the 50% sugarcane top bioplastic is the most favorable, with transparency value of 89% transmittance, tensile strength of 39.2 MPa, elongation percentage of 17.32%, density of 0.8444 g/cm3, thickness of 0.276 mm, water absorption of 98.36%, and biodegradability over 4 days. Based on the mechanical tests, the produced bioplastic holds potential for food packaging material.
Pemanfaatan Pati Sagu (Metroxylon sago) dan Limbah Kulit Jeruk Sebagai Bioplastik Pembungkus Makanan (Edible Film) Ramah Lingkungan Nirmala Fitria Firdhausi; Ajeng Ayu Ramadhani; Hawwa’ Cahya Maulida
Biotropic : The Journal of Tropical Biology Vol. 8 No. 1 (2024): Biotropic, Volume 8 Nomor 1, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i1.2086

Abstract

The increasing use of plastic harms the environment because plastic has properties that are difficult to degrade, resulting in environmental pollution, while the demand for plastic is increasing day by day. Bioplastics are a form of innovation from technological developments in modern times. One example of bioplastic is edible film which can function as food packaging that can be consumed. Edible film made from sago starch and orange pectin is believed to be able to produce edible films that have good characteristics. The results of this study, all nine treatment variations did not meet the standards for bioplastics that had been determined by SNI and JIS. The highest tensile strength values were obtained from testing variations in the concentration of 6% sago starch and 2% orange peel pectin (8,643 Mpa), while the highest elongation values were obtained from 4% sago starch and 2% orange peel pectin (5,33%.). Based on the results of the edible film using sago starch and pectin, it showed that more pectin content decrease the biodegradation rate.
Pengaruh Aplikasi Ekstrak Limbah Puntung Rokok Terhadap Serangan Hama Pada Pertanaman Edamame di Gunung Kupang, Banjarbaru Rika Norhasiaty Dewi; Noorkomala Sari; Untung Santoso
Biotropic : The Journal of Tropical Biology Vol. 8 No. 2 (2024): Biotropic, Volume 8 Nomor 2, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i2.2114

Abstract

Edamame cultivation in lndonesia has experienced various obstacles in it’s development, the main problem that edamame farmers often face is pest attacks. To overcome this problem, it is necessary to provide vegetable pesticides that do not leave negative residues for the environment or living creatures. One of materials that can be used is cigarette waste which there is tobacco that contains nicotine, phenol and eugenol. This compound is believed to control pests. The aim of this research is to determine the effect of application of cigarette waste extract on the intensity pest attacks and determine the best concentration for controlling edamame plant pests. This research used a Randomized Block Design (RAK) with 5 treatments, each treatment repeated 4 times so that a total of 20 experimental units were obtained. P0 = control, without cigarette waste extract treatment, P1 = 5% concentration, P2 = 10% concentration, P3 = 15% concentration, P4 = 20% concentration. The results of this study show that plants where cigarette waste extract was applied had a very significant effect on the intensity value of edamame pest attacks, while the best concentration for controlling edamame plant pests is cigarette waste extract with a concentration of 20% (P4).
Detection of Protein Cd2ap in Mice (Mus Musculus) Kidney Damage Induced by 4% NaCl Lia Septya; Ahmad Ridwan
Biotropic : The Journal of Tropical Biology Vol. 8 No. 2 (2024): Biotropic, Volume 8 Nomor 2, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i2.2135

Abstract

CD2-associated protein (CD2AP) is a podocyte protein and its presence in urine is consistent with changes in renal function index. Indications of proteinuria often characterized by Focal Segmental Glomerulosclerosis (FSGS). Purpose: To detect CD2AP as an early indicator and determine CD2AP concentration against the stages of kidney damage through three main parameters, quantification of urine CD2AP and serum creatinine confirmed by histopathology. The study involved 24 male mice divided into three Control and three Treatment Groups (4% NaCl induction) for two, four and six weeks. Quantitative analysis was carried out to determine the concentration of CD2AP using ELISA in urine and creatinine levels in serum using the Jaffe method followed by FSGS as qualitative analysis. Results: There were significant differences of CD2AP concentration between each treatment and control group (P≤0,05), whereas each treatment group showed differences but not significant (P≥0,05). The control and treatment group serum creatinine did not show a significant difference (P≥0,05). Kidney histopathology displays FSGS to indications of global sclerosis at the 4th-week and 6th-week treatment group. Conclusions: The presence of CD2AP in urine can be used as an early indicator of kidney damage but can not show a significant difference at each stage of kidney damage.
Pengaruh Sanitasi Air Bersih yang Ditinjau dari Keberadaan Fecal Coliform terhadap Kejadian Stunting Feliyanti; RA. Hoetary Tirta Amallia; RR. Rina Antasari; Delia Yusfarani
Biotropic : The Journal of Tropical Biology Vol. 8 No. 2 (2024): Biotropic, Volume 8 Nomor 2, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i2.2138

Abstract

Stunting is a global problem that is currently attracting world attention. The future of a nation lies in the quality of the nation's children today and a high incidence of stunting in a country can influence the progress of a nation in the future. Currently, an in-depth study is being carried out on the relationship between environmental sanitation and stunting, apart from lack of food. Therefore, this research aims to correlate the incidence of stunting with the quality of the water used. This research is a cross-sectional study using the quota sampling method. The research results show that there is a significant relationship between coliform numbers in drinking water and the incidence of stunting. Furthermore, a significant relationship was also found between the incidence of diarrhea and the presence of coliforms in drinking water. The results showed that of the 60 samples examined, consisting of 30 samples of water used by the stunted children group and 30 control samples, the coliform number in the WASH for the stunted children group was more than 1,100 CFU/100 mL, while in the non-stunted children group it ranged from 75-120 CFU/100 mL. Similarly, the average coliform count in drinking water consumed by the stunted children group was 4-75 CFU/100 mL, while for children who were not stunted it was 3-4 CFU/100 mL. The results showed a significant association between coliform counts in drinking water and the incidence of stunting, with a p-value of 0.000 which is smaller than alpha 0.05. Furthermore, there was also a significant association between the incidence of diarrhoea and the presence of coliform in drinking water, with a p-value of 0.000 which is smaller than alpha 0.05.
Potensi Ekstrak Kecambah Kacang Hijau (Phaseolus aureus) untuk Meningkatkan Viabilitas Spermatozoa akibat Paparan Monosodium Glutamat (MSG) Endah Mulyani; Artika Uthary; Rusdiyana Ekawati; Farida Nur’aini; Sulasthia; Dyah Eka Kurniawati; Nastiti Trikurniadewi
Biotropic : The Journal of Tropical Biology Vol. 8 No. 2 (2024): Biotropic, Volume 8 Nomor 2, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i2.2142

Abstract

Monosodium Glutamate (MSG) as an additional ingredient to enhance food taste has a negative impact on mice spermatozoa. This research aims to explore the potential of mung bean sprout extract as a therapeutic ingredient to increase the viability of mice spermatozoa exposed to a toxic dose of MSG (4 mg/g BW) for 35 days. This research was an experimental study with 5 experimental groups, namely a positive control group and a negative control group as well as 3 therapy groups with doses of mung bean sprout extract of 0,1 g, 0,2 g, dan 0,4 g peroral for 15 days.. The results of the ANOVA test showed that there were significant differences between treatment groups. As the dose of mung bean sprout extract increased, the percentage of spermatozoa viability also increased. This increase proved that the antioxidant content in mung bean sprout extract can inhibit oxidative stress triggered by MSG. Mung bean sprouts have the potential as a therapeutic ingredient to increase spermatozoa viability.            
Deteksi Gen Staphylococcal Enterotoxin Type A (SEA) dan Koagulase (coa) Staphylococcus aureus Pada Susu Sapi Mentah dari Peternakan Sapi Perah Wilayah Wonocolo Surabaya Endah Prayekti; Afrida Cahya Maharani; Ika Nur Aini
Biotropic : The Journal of Tropical Biology Vol. 9 No. 1 (2025): Biotropic, Volume 9 Nomor 1, 2025
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v9i1.2163

Abstract

Staphylococcus aureus is a pathogen that often contaminates raw cow's milk and involves various virulence factors during the tissue infection process such as enterotoxins and coagulase enzymes. Staphylococcal Enterotoxin Type A (SEA) and coagulase enzymes (coa) are important virulence factors of Staphylococcus aureus. SEA is a very potent gastrointestinal superantigen toxin and the presence of the SEA gene of Staphylococcus aureus in milk indicates a potential risk of food poisoning. Staphylococcus aureus is the most invasive species because it has a coagulase enzyme encoded by the coa gene as a defense against neutrophil white blood cell phagocytosis and contributes to the infection process. The purpose of this study was to detect the virulence factors of the SEA gene and coa gene of Staphylococcus aureus bacteria in raw cow's milk from farms in Wonocolo Surabaya to assess the safety of milked milk. The research method used was qualitative with a descriptive observational research type. Conventional microbiological testing of a total of 24 bacterial isolates obtained 5 isolates identified as Staphylococcus aureus. Staphylococcus aureus bacterial DNA isolates were analyzed molecularly using the Polymerase Chain Reaction (PCR) method and obtained 40% of positive samples for the SEA gene and 100% of negative samples for the coa gene. The results of coa gene detection showed negative even though the conventional test tube results obtained positive coagulation results. The failure of coa gene amplification in isolates was likely because this study used primers based on literature without designing its own primers and did not optimize PCR before analyzing samples.
Perbandingan Penggunaan Buffer CTAB dan Surfaktan ABS Untuk Isolasi DNA Candida albicans Althofur Hilman Rifki; Endah Prayekti
Biotropic : The Journal of Tropical Biology Vol. 9 No. 1 (2025): Biotropic, Volume 9 Nomor 1, 2025
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v9i1.2179

Abstract

This study aimed to compare the effectiveness of CTAB buffer and Alkyl Benzene Sulfonate (ABS) surfactant in isolating DNA from Candida albicans. The fungi were cultured in Sabouraud Dextrose Broth (SDB) for 3 days at 37°C, and the DNA isolation process was carried out through three stages: cell lysis, precipitation, and purification. The results of DNA isolation were evaluated qualitatively using electrophoresis and quantitatively using a Nanodrop Spectrophotometer. The results showed that DNA isolation was successful using Candida albicans CTAB buffer and Alkyl Benzene Sulfonate (ABS) surfactant. This was proven by visualization of genomic DNA bands from electrophoresis results and nanodrop spectrophotometer counts. The highest DNA concentration was found when using CTAB buffer with an average concentration of 65.5 ng/µl. Meanwhile, in ABS surfactant, the highest DNA concentration was found at a concentration of 12%, amounting to 31.3 ng/µl. DNA purity when using CTAB buffer and Alkyl Benzene Sulfonate (ABS) surfactant with a concentration of 12% resulted in pure results. in CTAB buffer the average DNA purity was 1.88. At a concentration of 12% Alkyl Benzene Sulfonate (ABS) surfactant, the average DNA purity was 1.81. From the results obtained, the Alkyl Benzene Sulfonate (ABS) surfactant cannot completely replace the CTAB buffer for Candida albicans DNA isolation. Alkyl Benzene Sulfonate (ABS) surfactant can be used as an alternative to CTAB buffer to obtain good DNA purity, but the concentration cannot match that of CTAB buffer.

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