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Esti Tyastirin
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Department of Biology, Faculty of Science and Technology UIN Sunan Ampel Surabaya Jend. A. Yani No. 117 street, Surabaya, Jawa Timur, 60237
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INDONESIA
BIOTROPIC
Published by Universitas Islam Negeri Sunan Ampel
ISSN : -     EISSN : 25805029     DOI : https://doi.org/10.29080/biotropic.v8i2
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropic : The Journal of Tropical Biology publish articles on South East Asia Tropical Biology from various perspectives, covering both literary and fieldwork studies. The Journal put empahis on aspects related to: Ecology Zoology Botany Microbiology Biology molecular Biospeleology Conservation and other tropical biology discourses Journals are published biannually in February and August. Editor invite all relevant parties to contribute in publishing sciencetific articles which have never been published
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 126 Documents
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The Detection of the Escherichia coli Gene in Well Water in Bojonegoro Using Polymerase Chain Reaction (PCR) Prayekti, Endah; Rosiana Dewi Regita; Khiliah Navis
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2449

Abstract

ABSTRACT Escherichia coli (E. coli) is an indicator of water contamination that can be pathogenic, especially the Enterotoxigenic E. coli (ETEC) pathotype, which carries the heat-resistant enterotoxin (est) gene. Well water that does not meet clean water quality standards can become a medium for Escherichia coli contamination, so it is important to perform molecular genetic detection. The study aimed to determine the presence of Escherichia coli bacteria and detect the est gene from well water isolates using the Polymerase Chain Reaction (PCR) method. Samples were collected from 19 wells using purposive sampling techniques. Escherichia coli identification was performed through conventional isolation, Gram staining, biochemical tests, and culture on selective media (EMB and MCA). The results of this study showed that four samples tested positive for Escherichia coli. Molecular detection using PCR for the est gene in Escherichia coli was not detected in any of the four Escherichia coli isolates. Based on these results, it was concluded that Escherichia coli was present in the samples, but the est gene was not detected. Keywords: Escherichia coli, est gene, well water, PCR ABSTRAK Escherichia coli (E. coli) merupakan indikator pencemaran air yang dapat bersifat patogen, terutama patotipe Enterotoxigenic E. coli (ETEC) yang membawa gen enterotoksin tahan panas (est). Air sumur yang tidak memenuhi standar kualitas air bersih dapat menjadi media kontaminasi Escherichia coli, sehingga penting dilakukan deteksi genetik secara molekuler. Penelitian bertujuan untuk mengetahui keberadaan bakteri Escherichia coli dan mendeteksi gen est dari isolat air sumur menggunakan metode Polymerase Chain Reaction (PCR). Sampel diambil dari 19 sumur menggunakan teknik purposive sampling. Identifikasi Escherichia coli dilakukan melalui isolasi konvensional, pewarnaan Gram, uji biokimia, dan kultur pada media selektif (EMB dan MCA). Hasil penelitian ini menunjukkan adanaya 4 sampel yang memiliki positif Escherichia coli. Deteksi molekuler dengan PCR terhadap gen est pada Escherichia coli tidak ditemukan pada keempat isolat Escherichia coli. Berdasarkan hasil penelitian ini disimpulkan bahwa pada sampel ditemukan Escherichia coli tetapi tidak ditemukan gen est. Kata Kunci: Escherichia coli, gen est, air sumur, PCR
Hubungan Kekerabatan Fenetik Melon Cantaloupe, Honey Globe, Devina, Luna, Sweetnett dan Hamigua Berdasarkan Karakter Kualitatif Ibnu Fajar, S.Pd., M.Sc, Muhammad; Wiwik Sri Untari; Shinta Nuriya Prayudi
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2452

Abstract

This study aims to determine the phenetic relationship of the six melons Cantaloupe, Honey Globe, Devina, Luna, Sweetnett and Hamigua based on quantitative phenotypic characters of melons. Research and research samples were taken and conducted at the Situbondo nugrafarm. The qualitative phenotypic characters observed consisted of 88 characters including root branching, epicotyl color, hypocotyl color, stem color, leaf shape, leaf color, leaf stalk color, flower color, ovary shape, fruit shape, fruit skin color, fruit stalk color, fruit flesh color, fruit flesh texture, fruit flesh taste, seed shape and seed coat color. Qualitative characters were processed into scoring data that were analyzed using MVSP software using the Jaccard Coefficient correlation coefficient. The dendogram was divided into 4 groups with a similarity index of 62%. The results of the study showed that group I, Devina melon is closely related to Luna melon, group III Cantaloupe melon is distantly related to group I, Devina melon and Luna melon, group II, Hamigua melon is closely related to Sweetneet melon, and group IV Honey Globe melon is distantly related to the five melon varieties. The qualitative phenotypic similarity of the six melons will be information for plant breeding. Keywords:  Melon, Qualitative Character, Kinship Relationship
Aktivitas Antibakteri Ekstrak Padina australis terhadap Isolat Bakteri Pembusuk Ikan Tongkol (Euthynnus affinis) Tamaya, Reni; Sriwulan
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2480

Abstract

Padina australis is a brown algae that has secondary metabolites such as phenols, tannins, flavonoids, saponins, and alkaloids that have antibacterial potential. This study aims to determine the antibacterial activity of Padina australis extract against spoilage bacteria of tuna (Euthynnus affinis) from genus Staphylococcus sp. and Bacillus sp. through disc diffusion method. Data on the diameter of the inhibition zone were analyzed statistically. The results showed that Padina australis extract was able to produce an inhibition zone in both isolates, but the antibacterial activity was relatively weak (≤ 5mm). The 60% extract concentration produced the highest inhibition zone of 2.07 mm in Staphylococcus sp. and 2.23 mm Bacillus sp. isolates, while the positive control produced strong to very strong antibacterial activity, with diameters of 19.28 mm and 33.76 mm. The results of statistical analysis showed that variations in extract concentration did not significantly affect the inhibition zone in both isolates (p = 0.119 and p = 0.160). The low antibacterial activity is likely due to limited content of bioactive compounds, the extraction method, and suboptimal diffusion of the extract on the agar medium. Padina australis extract has the potential as a natural antibacterial, but its effectiveness still needs to be improved with further optimization.
Studi Komposisi Bakteri dan Mikroalga pada Sistem Akuakultur Air Tawar Meyta Pratiwi; Atang; Aulidya Nurul Habibah; Rizal Khoirun Alfisah
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2527

Abstract

Freshwater aquaculture is still in demand by fish farmers to meet consumer demand for animal protein needs. Fish farming uses an intensive system to produce adequate amounts of fish, but it is followed by metabolite waste problems that can affect the quality of life of fish. Handling fish metabolite waste can be started by conducting a study of microbes that play a role in the decomposition of fish metabolite waste. The method used to determine the microbes is the survey method, by isolating culturable bacteria using nutrient agar (NA) and Winogradsky media, identifying microalga found in fish farming ponds, measuring the physico-chemical parameters of aquaculture pond water. The results obtained were five heterotrophic bacteria (106 cfu/mL), five nitrifying bacteria (105 cfu/mL), four phylum microalgae, and physico-chemical conditions of water (temperature 27 °C, TDS 76-78 mg/L, nitrates 2-2.7 mg/L, and pH 6.5-6.7). The microbes obtained are widely known to help the process of decomposing metabolite waste in fish farming ponds, so that they can be used as promised probiotic agents for aquaculture organisms in the future.
Analisis Reduksi Polutan Kromium (Cr) Menggunakan Agen Fitoremediasi Mangrove di Ekosistem Pesisir Tulehu Ambon Provinsi Maluku Asyik, Asyik Nur Allifah AF
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2535

Abstract

Mangroves are aquatic vegetation that act as phytoremediation agents due to their ability as biofilters, adsorbing, and accumulating pollutants in the environment, thus contributing to improving water quality. The detoxification mechanism of chromium pollutants carried out by mangroves is a cost-effective solution in addressing coastal environmental pollution. The research target focused on assessing the reduction capacity of mangroves against chromium pollutants in the coastal ecosystem of Tulehu Ambon, Maluku Province. This study was conducted for two months, from mid-Juny to mid-July 2025. Sampling of chromium pollutants found in mangrove roots, leaves, and sediments was carried out at three observation stations based on location characteristics. Chromium analysis was determined from the results of the Atomic Absorption Spectrophotometer (AAS) display. This study revealed that mangroves have the potential to reduce pollutants through deposition and stabilization processes, as indicated by FTD values ​​> 1, which indicate their role in phytostabilization mechanisms. The preservation and protection of mangrove forests must be a serious concern for the sustainability of coastal ecosystems in this area.
Identifikasi Bakteri Bioaerosol di Laboratorium Mikrobiologi UIN Sunan Ampel Surabaya Menggunakan Analisis Gen 16S rRNA Fadhillah, Ninik; Wifaqwudd Az-Zahra Rifti; Yuanita Rachmawati; Inggrit Tyautari; Ade Syafira Fatau; Muh. Ma’arif
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2540

Abstract

Bioaerosols are airborne particles containing living microorganisms or microbial fragments that can disperse in indoor environments. This study aimed to identify the morphological characteristics and community composition of airborne bacteria in three laboratories at UIN Sunan Ampel Surabaya using a combination of macroscopic and microscopic observations and 16S rRNA gene-based molecular analysis. Air samples were collected using the settle plate method and analyzed for spatial distribution, colony morphology, and taxonomic identification. Results showed variation in colony density among the laboratories, with Gram-positive bacteria (Staphylococcus and Bacillus) dominating at 71.4%, while Gram-negative bacteria (Pseudomonas and Moraxella) accounted for 28.6%. Molecular analysis confirmed species identification with sequence similarity ranging from 97.63% to 99.31%. These findings indicate that indoor bacterial communities are influenced by interactions between environmental sources and human activity. Proper ventilation management, surface hygiene, and biosafety procedures are essential to maintain air quality and minimize occupational health risks in laboratory settings.

Page 13 of 13 | Total Record : 126

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