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International Journal of Biomedical Science and Travel Medicine
Published by Universitas Warmadewa
ISSN : 30477441     EISSN : 30477433     DOI : https://doi.org/10.22225/ijbstm
Core Subject : Health,
Medicine & Pharmacology Public Health
International Journal of Biomedical Science and Travel Medicine is published twice (March and September) a year. The objective is to promote articles on biology and medicine. International Journal of Biomedical Science and Travel Medicine publishes original research work related to biological and medicine. The journal publishes Original articles, Short Reports, Case Reports, and Review articles. All articles published in International Journal of Biomedical Science and Travel Medicine (IJBSTM) are peer-reviewed and published online for immediate access and citation. International Journal of Biomedical Science and Travel Medicine (IJBSTM) publishes the Original clinical and experimental research studies, Review articles, Case reports on topics but not limited to: Biocemistry, Genetics and molecular biology, Medicine, Environmental health, Tropical diseases, Diseases in tropics, Global health, Pharmacological sciences, Biomedical sciences, Public health, Infection, Epidemiology and clinical epidemiology, Molecular biology, Microbiology, Travel Medicine
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
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Search results for , issue "52-59" : 1 Documents clear
Prevalence of qacE AND qacE[delta]1 resistance genes in pseudomonas species isolates from hospitals in Benin City, Nigeria Felix. A. Okunrobo; Helen. O Ogefere; Richard Omoregie
International Journal of Biomedical Science and Travel Medicine 52-59
Publisher : Publication Department, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/ijbstm.2.2.2025.52-59

Abstract

Background: The multi-drug efflux pump systems play a significant role in the mechanism of resistance to biocides in Pseudomonas species. This bacterium has been shown to harbor multi-drug transporter efflux systems genes involving QacE and QacE[delta]l, as do other gram negative bacteria. In Nigeria, the use of antimicrobial agents is unregulated, particularly the use of biocidal formulations both in health care facilities and for domestic purposes. Due to paucity of data on the distribution of qac genes in the health-care environment in Nigeria, this study aimed to determine the distribution of qacE and qacE[delta]1 genes among Pseudomonads–both aeruginosa and non-aeruginosa isolates, from clinical and environmental sources within hospitals in Benin City. Method: A total of 1200 specimens consisting of 500 clinical (wound, urine, ear swabs, high vaginal and endo-cervical swabs, eye swabs, aspirates, catheter tips, sputum and throat swabs) and 700 environmental (sinks, floors and bench tops) were used for this study. All specimens were processed to recover Pseudomonads using cetrimide agar. The isolates were identified with biochemical tests and 16S rRNA. The presence of qacE and qacE[delta]1 genes was detected by PCR (polymerase chain reaction). Result: Pseudomonas aeruginosa and non-aeruginosa isolates were recovered mostly from sinks and wounds. The prevalence of qacE and qacE[delta]1 genes did not differ significantly (p>0.05) between P. aeruginosa and their non-aeruginosa counterparts in both clinical and environmental isolates. In conclusion, we highlight the prevalence of qacE and qacE[delta]1 resistance genes in varying proportions among clinical and environmental pseudomonas species isolates in hospitals in Benin city, Nigeria  

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