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Jurnal Penelitian Saintek
ISSN : 14123991     EISSN : 25287036     DOI : 10.21831
Core Subject : Science,
Arjuna Subject : -
Articles 8 Documents
Search results for , issue "Vol 26, No 1 (2021)" : 8 Documents clear
VARIASI DAN HUBUNGAN FENETIK AKSESI KUNYIT DI YOGYAKARTA DAN SEKITARNYA Aditiana Vimala Guna; Purnomo Purnomo
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.36727

Abstract

Tujuan penelitian ini adalah menentukan variasi morfologis dan anatomis tiap aksesi Curcuma longa, serta hubungan fenetik pada spesies antar aksesi Curcuma longa. Sebanyak 13 sampel diambil di area Yogyakarta dan sekitarnya, karakter morfologis yang dibandingkan bersumber dari akar, rimpang, daun meliputi bentuk dan ukuran. Karakter anatomis diamati pada daun dan rimpang. Penentuan karakter morfologis berdasarkan Protection of Plant Varieties dan Farmers’ Rights Authority. Hubungan fenetik menggunakan perbandingan karakter morfologis dan anatomis spesies Curcuma longa yang diteliti. Hasil penelitian menunjukkan variasi morfologis Curcuma longa di Yogyakarta dan sekitarnya memiliki perbedaan tinggi tanaman, morfologis daun, serta morfologis akar dan rimpang. Variasi karakter anatomis Curcuma longa memiliki perbedaan ketebalan epidermis daun, ukuran dan densitas stomata, ketebalan berkas pengangkut, ketebalan epidermis dan hipodermis rimpang. Berdasarkan analisis fenetik Curcuma longa di Yogyakarta dan sekitarnya dapat diketahui bahwa hubungan kekerabatan fenetik berdasarkan karakter morfologis terbagi menjadi dua klaster besar dan empat subklaster, sedangkan berdasarkan karakter anatomis terbagi menjadi dua klaster dan enam subklaster.VARIATION AND PHENETIC RELATIONSHIP OF TUMERIC ACCESSIONS IN YOGYAKARTA AND SURROUNDING AREASThe purpose of this study was to determine the morphological and anatomical variations of each accession of Curcuma longa, as well as the phenetic relationship among accessions of Curcuma longa. A total of 13 samples were taken in the Yogyakarta and surrounding areas, the morphological characters compared from roots, rhizomes, leaves include shape and size. The anatomical characters were observed in the leaves and rhizomes. The determination of morphological characters based on Protection of Plant Varieties and Farmers’ Rights Authority. The phenetic relationship used a comparison of the morphological and anatomical characters of the Curcuma longa species studied. The results showed that the morphological variations of Curcuma longa in Yogyakarta and its surroundings had differences in plant height, leaf morphology, and root and rhizome morphology. Variations in anatomical characters of Curcuma longa have differences in leaf epidermis thickness, stomata size and density, carrier bundle thickness, epidermis and rhizome hypodermis thickness. Based on the phenetic analysis of Curcuma longa  in Yogyakarta and its surroundings, it shows phenetic relationship based on morphological characters is divided into two large clusters and four sub-clusters, while based on anatomical characters it is divided into two clusters and six sub-clusters. 
KEANEKARAGAMAN ORGANISME PADA RHIZOSFER GULMA SIAM DI LAHAN VULKANIK, PESISIR, DAN KARST Tien Aminatun; Siti Umniyatie; Anna Rakhmawati; Aji Suhandy; Nrangwesthi Widyaningrum; Kurnia Cahyani
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.38754

Abstract

Penelitian ini bertujuan untuk membandingkan kondisi edafik, hubungan antara kondisi edafik dan keanekaragaman organisme tanah, serta perbedaan keanekaragaman organisme tanah pada berbagai ekosistem rhizosfer gulma Siam (Chromolaena odorata) yang tumbuh di lahan vulkanik, pesisir, dan karst. Penelitian dilakukan dengan mengambil sampel tanah dari ekosistem rhizosfer gulma Siam di lahan vulkanik, karst, dan pesisir Daerah Istimewa Yogyakarta; mengamati komposisi organisme tanah di laboratorium, yaitu collembola, nematoda, dan mikoriza. Hasil penelitian menunjukkan bahwa tanah pada rhizosfer gulma Siam yang tumbuh di lahan karst menunjukkan tingkat kesuburan tertinggi yang ditunjukkan oleh kandungan N, K, dan C-organik tanah, sedangkan tanah pesisir memiliki kandungan P tertinggi. Tekstur tanah pada rhizosfer gulma Siam lebih berpengaruh terhadap keanekaragaman organisme tanah dibandingkan kandungan hara tanah dan sifat tanah lainnya yang diteliti. Keragaman organisme tanah rhizosfer gulma Siam lebih besar di lahan pesisir dibandingkan di lahan karst dan vulkanik. Implikasi dari hasil tersebut adalah bahwa tanah dengan tekstur berpasir akan lebih bermanfaat bagi pertumbuhan gulma Siam dengan keanekaragaman organisme rhizosfer yang lebih tinggi.ORGANISM DIVERSITY IN THE RHIZOSPHERE OF SIAM WEEDS IN VOLCANIC, COASTAL AND KARST LANDThe research aims to compare the edaphic condition, the relationship between the edaphic condition and soil organism diversity, and the differences of soil organism diversity in different rhizosphere ecosystems of Siam weed growing in volcanic, coastal and karts areas. The research was conducted by taking soil samples from the Siam weed rhizosphere ecosystems in volcanic, karst and coastal areas of the Special Region of Yogyakarta and observing the composition of the soil organisms in the laboratory, including collembola, nematode, and mycorrhiza. The results find that soil from rhizosphere of Siam weed growing in karst area shows the highest level of fertility indicated by the N, K, and C-organic contents of the soil, whereas soil from coastal area has the highest P content. Soil texture in the rhizosphere of Siam weed has more effect on the diversity of soil organisms than the soil nutrient content and other soil properties investigated. The diversity of soil organisms of Siam weed rhizosphere is greater in coastal area than those in karst and volcanic areas. The implication of the results is that soil with a sandy texture will be more beneficial for the growth of Siam weed with a higher diversity of rhizosphere organisms
EXPLORING THE POTENTIAL ENERGY OF LODAGUNG MINI-HYDROPOWER PLANT IN BLITAR, WEST JAVA, INDONESIA Etty Susilowati; Raymond Valiant Ruritan
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.35248

Abstract

Renewable energy in Indonesia is not optimally developed. Most existing renewable energy power plants come from water and geothermal, but the utilized capacity is far away from significant. Lodagung mini-hydro power plant was built by The Public Corporation, Jasa Tirta I  with an installed capacity of 2x650 kW utilizing water from the Lodagung Irrigation Channel, Wlingi Dam to generate electricity in Blitar Regency, East Java. The plant has become the first to use Siphon technology in its operation. This study aims to analyse the potential power generated by Lodagung Mini-hydropower plant using primary and secondary data. The data processing was done by calculating the reliable discharge of the irrigation channel for three years since 2011 to 2013; calculating the water discharge in the siphon pipe; and calculating the estimated power generated by the plant. The findings indicate that the maximum discharge of the Lodagung irrigation channel is 13.78 m3/second, and the minimum discharge is 8.89 m3/second. The design discharge is 13.78 m3/second, and the maximum discharge in the siphon pipe is 3.96 m3/second/pipe. Based on the need, the expected power generated ranges from 16.48 kWh/day to 28.66 kWh/day.
POTENSI INFUSA DAUN NANGKA SEBAGAI OBAT KUMUR HERBAL Christ Alfianus Tosubu; Nunung Sulistyani; Nur Khikmah
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.38032

Abstract

Penelitian ini dilakukan untuk mengkaji potensi daun nangka (Artocarpus heterophyllus) sebagai obat kumur herbal dengan melihat jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Daun nangka berwarna hijau muda yang diperoleh dari Babadan, Banguntapan, Bantul dibuat simplisia. Penentuan potensi dilakukan dengan menentukan perbedaan jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Uji potensi infusa daun nangka sebagai obat kumur dilakukan dengan menghitung perbedaan jumlah koloni bakteri rongga yang diperoleh dengan melakukan swab pada pangkal lidah sebelum dan sesudah berkumur. Perhitungan koloni bakteri dilakukan menggunakan metode hitung cawan (total plate count) pada media plate count agar (PCA). Hasil penelitian menunjukkan rata-rata jumlah koloni bakteri sebelum dan sesudah berkumur dengan infusa daun nangka yaitu 1,78 x 107 CFU/mL dan 7,71 x 106 CFU/mL. Perlakuan pemberian obat kumur infusa daun nangka secara signifikan mampu menurunkan jumlah koloni bakteri rongga mulut. Infusa daun nangka berpotensi sebagai alternatif obat kumur herbal. THE POTENCY OF JACKFRUIT LEAF INFUSION AS A HERBAL MOUTHWASHThis study was conducted to examine the potential of jackfruit (Artocarpus heterophyllus) leaves as a herbal mouthwash by identifying the growth of bacterial colonies in the oral cavity before and after gargling with jackfruit leaf infusion. The light green jackfruit leaves obtained from Babadan, Banguntapan, Bantul were made simplicia. The determination of potency was done by determining the difference in the number of bacterial colony growth in the oral cavity before and after gargling with jackfruit leaf infusion. The potential test of jackfruit leaf infusion as a mouthwash was carried out by calculating the difference in the number of cavity bacterial colonies obtained by swab at the base of the tongue before and after gargling. Bacterial colonies were counted using the total plate count method on plate count agar (PCA) media. The results showed that the average number of bacterial colonies before and after rinsing with jackfruit leaf infusion was 1.78 x 107 CFU/mL and 7.71 x 106 CFU/mL. The treatment of giving jackfruit leaf infusion mouthwash was significantly able to reduce the number of bacterial colonies in the oral cavity. Jackfruit leaf infusion has the potential as an alternative to herbal mouthwash.
PENENTUAN AKTIVITAS EKSTRAK ETANOL CABAI MERAH DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Alfi Sapitri; Eva Diansari Marbun; Ulfayani Mayasari
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.39859

Abstract

Penelitian ini bertujuan untuk melakukan uji fitokimia terhadap ekstrak etanol Cabai merah (Capsicum annum L.) dan uji aktivitasnya terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian aktivitas antibakteri ini menggunakan metode difusi agar dengan menggunakan kertas cakram dengan tiga kali pengulangan. Ekstrak etanol cabai merah dibuat dengan konsentrasi 45, 60, 75, dan 90%. Uji aktivitas antibakteri dilakukan dengan metode Kirby Bauer. Media yang digunakan sebagai uji antibakteri pada penelitian ini yaitu Mueller-Hinton Agar (MHA). Hasil skrining fitokimia terhadap ekstrak etanol cabai merah menunjukkan adanya senyawa metabolit sekunder. Senyawa metabolit sekunder tersebut yaitu alkaloid, flavonoid, tanin, saponin, dan Steroid/triterpenoid. Steroid berfungsi sebagai antimikroba. Hasil penelitian ini juga mengungkapkan adanya kandungan senyawa capsaicin yang terkenal sebagai senyawa aktif antimikroba. Ekstrak etanol cabai merah memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli zona bening terendah pada konsentrasi 45% dan zona bening tertinggi pada konsentrasi 90%. Dapat disimpulkan bahwa ekstrak cabai merah memiliki aktivitas antibakteri terhadap penghambatan pertumbuhan bakteri.THE ABILITY OF RED CHILI ETHANOL EXTRACT IN INHIBITHING THE GROWTH OF BACTERIAThis study aimed to perform a phytochemical test on the ethanolic extract of red chili (Capsicum annum L.) and test its activity against the growth of Staphylococcus aureus and Escherichia coli bacteria. This antibacterial activity test used the agar diffusion method using disc paper with three repetitions. Red chili ethanol extract was made with concentrations of 45%, 60%, 75% and 90%. The antibacterial activity test was carried out using the Kirby Bauer method. The medium used as an antibacterial test in this study was Mueller-Hinton Agar (MHA). The results of phytochemical screening of red chili ethanol extract showed the presence of secondary metabolites. The secondary metabolites are alkaloids, flavonoids, tannins, saponins, and steroids/triterpenoids. Steroids serve as  antimicrobials. The results of this study also revealed the presence of capsaicin compounds which are known as active antimicrobial compounds. Red chili ethanol extract had antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the lowest clear zone at a concentration of 45% and the highest clear zone at a concentration of 90%. It can be concluded that red chili extract has antibacterial activity against bacterial growth inhibition.
PERBANDINGAN AKTIVITAS SITOTOKSIK EKSTRAK DAN MINYAK ATSIRI RIMPANG Curcuma mangga Val. TERHADAP SEL MCF-7 Purwanto Purwanto; Putri Khaerani Cahyaningrum; Retno Sunarminingsih Sudibyo
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.39977

Abstract

Rimpang Curcuma mangga Val. banyak digunakan sebagai obat herbal antikanker payudara. Penelitian aktivitas sitotoksik terhadap sel kanker payudara banyak dilakukan utamanya untuk minyak atsiri rimpang, dan hanya sedikit penelitian terhadap ekstraknya. Walaupun demikian belum ada yang membandingkan aktivitas sitotoksik dari ekstrak dan minyak atsiri tersebut terhadap sel kanker payudara; meskipun kandungan senyawa keduanya berbeda. Oleh karena itu penelitian ini bertujuan membandingkan aktivitas sitotoksik dari ekstrak dan minyak atsiri rimpang C. mangga Val. secara in vitro terhadap sel kanker payudara MCF7. Ekstrak rimpang dibuat secara maserasi menggunakan pelarut n-heksana; sedangkan minyak atsiri dibuat melalui destilasi uap irisan rimpang selama 5 jam. Uji aktivitas sitotoksik in vitro dilakukan menggunakan metoda MTT Assay. Rendemen minyak dari ekstrak n-heksana rimpang C. mangga Val. adalah 1,15 x 10-2 % sedangkan rendemen minyak atsiri adalah 6,3 x 10-2 %. Hasil uji sitotoksik menghasilkan IC50 ekstrak 106,414 µg/ml (R2=0,9677) dan minyak atsiri 198,557 µg/ml (R2=0,8037). Hal ini menunjukkan bahwa ekstrak rimpang C. mangga Val. lebih sitotoksik terhadap sel kanker payudara MCF-7 daripada minyak atsirinya, karena kandungan ekstrak mayoritas diterpenoid (53,18%) sedangkan minyak atsiri mayoritas monoterpenoid (51,34%).THE COMPARISON BETWEEN THE ACTIVITIES OF CYTOTOXIC EXTRACTS AND ESSENTIAL OILS OF RHIZOME Curcuma mango Val. TOWARD MCF-7 CELLSCurcuma mangga Val. rhizome has been used as herbal anti breast cancer. Researches on cytotoxic activity towards breast cancer cells have been done especially to the rhizome’s essential oil; and only few researches done to the extract. However there is no cytotoxic activity comparation of the extract and essential oil towards breast cancer cells; even tough their substance contents are different. Therefore, this study aimed to compare the cytotoxic activity in vitro of the extract and essential oil of C. mangga Val. rhizomes towards breast cancer cells of MCF-7. The rhizome extract was prepared by maceration using N-hexane; while the essential oil was prepared by steam distillation for 5 hours of the sliced rhizomes. The in vitro cytotoxic test was carried out using MTT Assay. The yield of oil from rhizome extract was 1.15 x 10-2 %; while the yield of essential oil was 6.3 x 10-2 %. The IC50 of extract oil was 106.414 µg/ml (R2=0.9677) and the IC50 of essential oil was 198.557 µg/ml (R2=0.8037). It shows that rhizome extract of C. mangga Val. was more cytotoxic towards MCF-7 than the oil because the majority content of extract were diterpenoids (53.18%) while the oil were monoterpenoids (51.34%).
KAJIAN POTENSI ANTIOKSIDAN ALAMI BERBAGAI LIMBAH TANAMAN Rukiah Lubis; Nur Hidayah; Nopriyeni Nopriyeni
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.39527

Abstract

Tujuan dari penelitian ini adalah untuk menganalisis kandungan antioksidan (antosianin, vitamin A, C, dan E) dari limbah kulit buah senduduk (Melastoma malabathricum L.), jantung pisang, jengkol. Penelitian dilaksanakan selama enam bulan. Analisa antosianin dan vitamin A, C, dan E dilakukan di Balai Besar Industri Agro, Bogor. Bahan yang digunakan pada penelitian ini terdiri dari kulit buah senduduk, jantung pisang, dan jengkol. Analisis kandungan vitamin A dilakukan pada sampel mengunakan alat High Performance Liquid Cromatography (HPLC). Penentuan  kadar  antosianin  dihitung menggunakan metode perbedaan pH. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa aktivitas antioksidan alami yang paling tinggi menurut analisis kandungan vitamin A, C, E dan antosianin dari ketiga limbah tanaman (buah senduduk, jantung pisang, kulit jengkol), terdapat pada kulit buah senduduk dan kulit jantung pisang yaitu kandungan vitamin C (111.33 mg/kg dan 263.33 mg/kg) dan kandungan antosianin (684.008 mg/100g dan 174.289 mg/100g). Dapat disimpulkan bahwa buah senduduk dan jantung pisang bisa dijadikan sebagai antioksidan alami yang baik berdasarkan hasil analisis kandungan vitamin A, C, E, dan kandungan antosianin.THE STUDY OF POTENTIAL NATURAL ANTIOXIDANTS FROM PLANT WASTEThis study was aimed at analyzing the content of antioxidants (anthocyanins, vitamins A, C, and E) from the rind of sanduduk’s fruit (Melastoma malabathricum L.), Blossom of the banana plant (Musa acuminata Colla), jengkol (Archidendron pauciflorum) fruit. The research was conducted for six months. Analysis of anthocyanins and vitamins A, C, and E was performed at Balai Besar Industri Agro, Bogor. The materials used in this research consisted of sanduduk rind, banana blossom, and jengkol fruit. Vitamin A content analysis was performed on the samples using High Performance Liquid Chromatography (HPLC). The determination of anthocyanin levels was calculated using the pH difference method. The data obtained were analyzed descriptively. The results showed that the highest natural antioxidant activity according to the analysis of vitamin A, C, E and anthocyanin content from the three wastes, found in sanduduk rind and banana blossom which contain vitamin C ( 111.33 mg/kg and 263.33 mg/kg) and anthocyanin (684.008 mg/100g and 174.289 mg/100g). It can be concluded that sanduduk fruit and banana blossom can be used as a good natural antioxidant based on the results of analysis of vitamin A, C, E, and anthocyanin content.
ANALISIS POTENSI ENERGI TERBARUKAN PEMBANGKIT LISTRIK TENAGA MIKROHIDRO DI MANOKWARI SELATAN Elias Kondorura Bawan; Antonius D. Palintin; Eric A. Patandianan
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.37311

Abstract

Tujuan dari penelitian ini adalah menganalisis potensi energi listrik di kabupaten Manokwari Selatan. Penelitian dilaksanakan pada bulan Juli 2019 di Kabupaten Manokwari Selatan pada tiga Distrik. Survei geologi dilakukan dengan pendekatan geologi regional Lembar Ransiki. Untuk analisis hidrologi dan kemiringan lereng dilakukan dengan menggunakan citra satelit, Digital Elevation Model (DEM) dan Peta Rupa Bumi Indonesia (RBI) dan aplikasi Sistem Informasi Geografis (SIG) serta pengukuran di lapangan untuk penentuan debit lapangan. Kondisi hidrologi yang menjadi kajian meliputi potensi debit dan curah hujan. Perhitungan debit air pada DAS menggunakan metode FJ. Mock. Data evapotranspirasi potensial dihitung dengan menggunakan metode Pennman Modifikasi. Penelitian ini menunjukkan bahwa debit andalan pada sungai Susmorof mencapai 1500 L/dt masing-masing untuk kepentingan pengembangan sistem. Total potensi PLTMH di dua lokasi yang dikaji adalah sebesar 30,4 kW di sungai Bengko kampung Sihu dan 73,4 kW. Untuk pengembangan PLTMH di Kampung Susmorof hal ini penting untuk ditindaklanjuti pada tahapan studi kelayakan dan penyusunan perencanaan detail desain.THE ANALYSIS OF RENEWABLE ENERGY POTENTIAL OF MICROHYDRO POWER PLANT IN SOUTH MANOKWARIThe purpose of this study was to analyze the potency of electrical energy in South Manokwari district. The research was conducted in July 2019 in South Manokwari Regency in three districts. The geological survey was carried out using the Ransiki Sheet regional geological approach. For hydrological and slope analysis, satellite imagery, Digital Elevation Model (DEM) and Indonesian Earth Map (RBI) and Geographic Information System (GIS) applications as well as measurements in the field were used to determine field discharge. The hydrological conditions under study include the potential for discharge and rainfall. Calculation of water discharge in the watershed uses the FJ Mock method. Potential evapotranspiration data were calculated using the Modified Pennman method. This study shows that the mainstay discharge in the Susmorof river reaches 1500 L/s each for the purpose of system development. The total potential for MHP in the two locations studied are 30.4 kW in the Bengko river, Sihu village and 73.4 kW. For the development of MHP in Susmorof Village, this is important to be followed up at the stage of feasibility studies and preparation of detailed design planning.   

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