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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol. 3 No. 3 Juli 2014" : 8 Documents clear
BIOPROSPEKSI RHIZOBAKTERI PENGHASIL IAA (Indole Acetic Acid) DARI TANAMAN JAGUNG (Zea maysL.) DI AREA PERTANIAN SEMI ORGANIK DESA BATURKEC. GETASAN KAB. SEMARANG Khoirul Huda; Anto Budiharjo; Budi Raharjo
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Microorganisms in nature have a rich diversity and have an important role in human lives, especially in agriculture. Some types of bacteria live in the area of plant roots called rhizobacteria. Some rhizobacteria has the ability to stimulate the growth of crops such as produce IAA (Indole Acetic Acid). This study aims to find rhizobacteria in maize (Zea mays L.) which has the ability to produce IAA (Indole Acetic Acid) that can be used as a reference in rhizobacteria resource utilization to increase agricultural production in a sustainable and environmentally friendly.This study was conducted with bacterial isolation, characterization of bacterial isolates in colony and cell morphology, The test of rhizobacteria’s ability to produce IAA (Indole Acetic Acid), the molecular identification of the isolates were able to produce IAA rhizobacteria and confirmatory tests. The results of isolation obtained seventeen isolates of rhizobacteria where there is a one of isolate (J.6) is able to produce 20.5 ppm IAA in TSB (Tryptic Soy Broth) supplemented with 100 ppm of L-tryptophan. The Result of the molecular identification show that isolate which has the ability to produce IAA has 97% similarity with Bacillus safensis Strain A-2. Isolate “J.6” have the same characteristic features with Bacillus safensis. They are a gram-positive, rod-shaped, capable of forming endospores, motile, catalase positive, capable of fermenting glucose and hydrolyzing a starch. Key word : Rhizobacteria, Indole Acetic Acid, Bacillus safensis Strain A-2
PENGARUH PERBEDAANKADAR LOGAM BERATKROMIUM (Cr) TERHADAPPERTUMBUHAN POPULASISpirulina platensis (Gomont) Geitler DALAM SKALA LABORATORIUM Ahmad Yusuf Afandi; Tri Retnaningsih Soeprobowati; Riche Hariyati
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Industrial products such as metal and paint had generate hazardous wastes, one of then is a heavy metal Chromium (Cr). Industrial wastes containing Cr may cause many negative effects when directly discharged to environment without any prior processing. Therefore, recovery actions (remediation) is one of many ways to solve the problem. Phycoremediation is one of remediation that is simple, efficient and safe in process by using the microalgae to remediate the environment, and one of the microalgae is Spirulina platensis (Gomont) Geitler. This study was conducted to determine the effect and the removal efficiency of heavy metals Cr on Spirulina platensis culture media. The results showed there was significant influence between the administration of heavy metal Cr 1 mg/l, 3 mg/l and 5 mg/l Cr on the population growth of S. platensis. Heavy metal Cr concentration of 1 mg/l increased the growth of S. platensis, while the opposite happened when administrated with 3 mg/l and 5 mg/l. Higher concentration of heavy metal Cr on culture media decreased population of S. platensis. The culture added with heavy metal Cr concentration of 1 mg/l, 3 mg/l and 5 mg/l on the 7 days treatment were able to decrease heavy metal Cr level respectively 35%, 14% and 8%. Thus, the longer treatment days the bigger decrease percentage concentration of heavy metal Cr. Keywords: Cr, phycoremediation, microlagae, Spirulina platensis
POTENSI RHIZOBAKTERI DARI TANAMAN KUBIS (Brassica oleracea var. capitata L.) DAERAH GETASAN SEMARANG SEBAGAI AGEN BIOBAKTERISIDA TERHADAP PATOGEN Xanthomonas campestris Maya Fitriana Ilul Fahmi; Anto Budiharjo; Agung Suprihadi
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rhizobacteria are bacteria that live around plant roots but do not cause negative effects on their host and are known to act as a biobactericide agent. The use of chemical-based pesticide against plant pathogen can be replaced with rhizobacteria. The objectives of this research were to isolate rhizobacteria from cabbage, explored from Getasan, Semarang  and to determine the ability of the isolates to inhibit pathogens Xanthomonas campestris causing black rot on cabbage in vitro. The research concisted of  rhizobacterial isolation, morphologic bacterial characterization, antibacterial test, and molecular and biochemical identification of the isolates. The isolation obtained seventeen rhizobacterial isolates. Four isolates (K.1, K.3, K.9 and K.12) showed potency as an biobactericide agent against pathogenic X. campestris. K.9 had the best ability to inhibit the growth of X.campestris by 12,6 mm. Based on molecular identification K.9 was Bacillus cereus strains BF15. Morphology and Biochemistry test showed that isolates K.9 is gram positive bacteria shaped bacilli, able to form an endospore, positive in hydrolysing starch , fermentation glucose, motile, aerobic and negative in the fermentation of manitol and arabinosa. Keywords : Antibacterial, Rhizobacteria, Cabbages, black rot disease, Xanthomonas. campestris
POTENSI RIZOBAKTERI PEMBENTUK ENDOSPORA DARI TANAMAN PADI SEBAGAI BIOKONTROL FITOPATOGEN Xanthomonas oryzae Maerani Sumarno; Anto Budiharjo; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Xanthomonas oryzae is phytopathogen causing bacterial leaf blight which decreases in agricultural product reaching 20-70 % in Asia. Bacterial leaf blight symptoms is characterized by the formation of lines in the leaf blade turnings yellow, then white, causing the plant to wither and die. Endospore-forming rhizobacteria are soil microbes potential as biocontrol to inhibit phytopathogen growth. The aims of this study were to isolate endospore-forming rhizobacteria from rice plant and determine its ability as biocontrol against X. oryzae. The methods used consisted of isolation, antibacterial activity test, molecular identification, and biochemical characterization. Twenty isolates of endospore-forming rhizobacteria were obtained from the isolation of the rice crop. Isolate P-10 had the greatest ability against X. oryzae with inhibition zone of 18.89 mm. Molecular identification using 16S rRNA gene showed that isolates P-10 had 98 % homology with Bacillus pumilus. Biochemical characterization showed the isolate P-10 had a rod-shaped with center of endospores, gram-positive, catalase positive, are motile, negative in starch hydrolyze, not forming gas on glucose, these characteristics fitted with B. pumilus character. Keywords : Rice plant, antibacterial, Xanthomonas oryzae, endospore-forming, rhizobacteria
Interaksi antara Tingkat Ketersediaan Air dan Varietas, Terhadap Kandungan Prolin serta Pertumbuhan Tanaman Kedelai (Glycine max L. Merr) Eko Wahono; Munifatul Izzati; Sarjana Parman
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The Soybean production in Indonesia has decreased due to erratic weather conditions. It is necessary to plant soybeans that are tolerant to drought stress to overcome this problem. Soybean adapt to drought stress by accumulating proline to protect cells from damage. The purpose of this study was to determine the effect of water availability on growth and proline content in soybean. This study was conducted in Greenhouse Gombel Lama and Biology Laboratory Diponegoro University. The design used was a completely randomized design (CRD) with 2 factors these are the level of water availability and soybean plant variety.  This research had 6 treatments and 3 replication. Data were analyzed using ANOVA, followed by Duncan's multiple range real difference test. Parameters measured were proline content and plant growth (plant height, fresh and dry weight of shoot and roots). The results show that the level of water availability affect the content of proline and growth. The lowest water availability had the highest prolin content, that was Grobogan variety with 2,15 µmol/gr of proline content while Wilis variety had 2.30 µmol/gr of proline content. The soybean plant variety not significantly affect the content of proline and plant growth, but significantly affect on plant height and number of leaves trifoliat. Grobogan variety was more adapted to drought stress than Wilis variety.Key words : proline, water availabyility, soybean, Glycine max
EKSPLORASI RHIZOBAKTERI INDIGENOUS TANAMAN CABAI RAWIT (Capsicum frustescens Linn.) DARI PERTANIAN SEMI ORGANIK DESA BATUR KABUPATEN SEMARANG SEBAGAI AGEN HAYATI PENGENDALI PERTUMBUHAN JAMUR Fusarium oxysporum f.sp capsici M Eka Prastya; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rhizobacteria is a group of bacteria that live in the around of plant roots. This type of bacteria known have the ability to stimulate plant growth by producing growth hormone, as well as to inhibit the growth of plant pathogens synthesize compounds with antibiotics or extracellular enzymes. The purpose of this study was to obtain and describe the morphological, biochemical and genetic isolates rhizobacteria of semi-organic farmland Semarang District village Batur which has the ability as a biological control agent of fungal pathogens Fusarium oxysporum f.sp capsici. The results obtained fifteen isolates the majority rhizobacteria bacilus shaped gram-positive and classified. Rhizobacteria inhibition test capabilities against pathogenic fungi was performed using dual culture test and test biomass. Dual culture test results showed that the inhibition of isolates E1 has a 3.77%, 1.88% isolates E3 and E15 isolates 22%. The biomass tests show E15 isolates capable of inhibiting the growth of pathogenic fungi best with the smallest weight of fungal biomass 0.0386 grams. The results of the molecular characterization based on 16S rRNA gene sequences to known that E15 isolates has similar with Bacillus cereus strain ATCC 14579 with similarity of 97%. The results of the biochemical characterization of isolates E15 has similarities with B. cereus species that is catalase positive, motile, have endospores, is able to hydrolyze starch and ferment glucose.Keywords: rhizobacteria, Fusarium oxysporum f.sp capsici, gen 16S rRNA, Bacillus cereus
PEMBERIAN KOMBINASI MIKROMINERAL (Cu, Fe, Zn, Co) DAN VITAMIN (A, B1, B12, C) MELALUI DRINKING WATER TERHADAP PERTUMBUHAN PUYUH ( Coturnix-coturnix japonica L) Sofatin Fitriyani; Koen Praseno; Silvana Tana
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

This aim of this research was to determine the potential of giving micromineral solution (Cu, Fe, Zn, Co) and vitamins (A, B12, B1, C) as drinking water by somatometrik. The method used is a combination gift mikromineral (Cu, Fe, Zn, Co) and vitamins (A, B12, B1, C) with normal doses, twice the normal dose, four times the normal dose, and which is a combination mikromineral and vitamins. The parameters in this research were body weight and somatometrik. This research used Completely Randomized Design (CRD). The data were analyzed with Analysis of variants (ANOVA) with the help of the SAS program, the results of which showed significant, then proceed to further test, using Duncan test. The analysis showed significant difference in body weight gain, femur length, tibia length of tarsus and metatarsus Tarso length, and do not differ significantly in the length of the beak, wing length, feed consumption and water consumption. Giving combination mikromineral (Cu, Fe, Zn, Co) and vitamins (A, B12, B1, C) as a drinking water has the potential to be used as an alternative in the management of quail with respect to maintenance doses given.Key words : vitamins, microminerals, growth, quail (Coturnix coturnix japonica L.)
EFEKTIVITAS PENGAWETAN SECARA OZONISASI DAN IDENTIFIKASI TERHADAP KONTAMINASI KAPANG PADA KACANG TANAH (Arachis hypogaea L.) Syawal Nurangga Kodhatin; Endang Kusdiyantini; Arina Tri Lunggani
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Peanut (Arachis hypogaea L.) is a potential plant protein source with a relatively cheap price, so that it can be consumed by almost all the community. Post-harvest handling of peanuts especially at the stage of the marketing needs to be done properly, because improper treatment can lead to contamination by fungi. As a result, it can reduce the quantity and quality of the product and can endanger the health of consumers. This study aims to determine the Ozonisation Preservation and Identification of Mold Contamination In Peanuts. Mold was isolated using the direct method (direct method) and dilution. Identification of molds were done macroscopically and microscopically. Identification was done by using Potato Dextrose Agar (PDA) and Malt Extract agar (MEA) as selective media. The result of Ozonisation Preservation with 3.5 ppm concentration showed that peanuts that had been exposed with ozone on first day containing the amount of molds lower than on the day 7, 14, 21, and 28. The result of mold identification on peanuts consisted of one genus Aspergillus with predominant isolate was A. flavus as many as 25 colonies. Keywords: peanut (Arachis hypogaea L.), ozonation, identification, mold

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