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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol. 4 No. 3 Juli 2015" : 8 Documents clear
Fermentasi Kopi Robusta (Coffea canephora) Menggunakan Isolat Bakteri Asam Laktat Dari Feces Luwak Dengan Perlakuan Lama Waktu Inkubasi Doni Usman; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Coffee is one of Indonesian main corps commodity. Caffeine content of robusta coffee is 1.6%-2.4% higher than arabica coffee that was 0.9-1.2%. Coffee fermentation was one of method to decrease caffeine content. Luwak coffee is coffee that produces by luwak use their digest system in luwak body and defecates as faeces. Lactic acid bacteria (LAB) exploration from luwak faeces that fed by robusta coffee is one of method to get lactic acid bacteria. This research aimed was lactic acid bacteria isolation from luwak faeces and determine best incubation time on robusta coffee fermentation. Bacteria isolation was resulted 27 isolate. Biochemical test done by Gram Staining Test, Catalase Test, Motility Test, and Acid Producing Test and resulted 13 LAB isolate. Characterization test done to all of LAB isolate and resulted best 3 isolate. Isolate M6 was xylanolitic potency, M8 was Proteolytic potentcy and m16 was cellulolytic potency. Robusta coffee fermentation done used mixed of M8, M6 and M16 as inoculum. This research use Complete Random Design (RAL), the treatment was fermentation time 8 hour (D1), 16 hour (D2) and 24 hour (D3), each treatment replays 3 times. Kontrol of this fermentation is robusta coffee without fermentation treatment (control 1) and luwak robusta coffee  (control 2). Data analysed by Analysis of Variance (ANOVA) continuing by Duncan Test with significance rate was 95%. This research result shown that fermentation time duration was cause decrease of caffein content, coffee beans weight and fermentation fluid pH. Highest caffeine content on D1 was 0.95%, and D3 was 0.72 %.Keyword: Luwak faeces, lactic acid bacteria, gram staining, fermentation, exploration
Identifikasi Isolat Aspergillus sp. KRM 43 dari Madura dan Produksi Enzim Protease dengan Variasi pH dan Waktu Inkubasi Nopvita Windi Astuti; MG. Isworo Rukmi; w wijanarka
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Proteases is an enzyme that has a high economic value, because they widely used for application in the field of industry.Protease can be generated from microorganisms, one of protease producer is derived from the genus Aspergillus. Aspergillus sp. KRM 43 that has isolated from alkaline soil can be predicted capable to produce alkaline protease. The purpose of this study was  to know the optimum of pH and incubation time for enzyme production from the mould. This research was conducted using a  factorial Randomized Complete Block Design with two factor i.e. a pH of 7, 8, 9 and incubation time of 5, 6, 7 days. Data collected were analyzed using Anova (α 0,05). The mould isolate were identified by observing its macroscopic and microscopic morphology. The result showed that Aspergillus sp. KRM 43 was identified as Aspergillus parasiticus. In this study showed pH treatment hadn't been  able to affect the production of protease. The highest proteases alkaline production of A. parasiticus KRM 43, found at 7 days incubation with proteases activity 2.24 U/mL and the specific activity 7.23 U/mg.Key words: Proteases, Aspergillus parasiticus, pH, incubation time
Kualitas Air Rawa Jombor Klaten, Jawa Tengah Berdasarkan Komunitas Fitoplankton Ayu Ambar Alina; Tri Retnaningsih Soeprobowati; Fuad Muhammad
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Phytoplankton have an important role in the aquatic ecosystem as a primary producer.   Phytoplankton sensitive to the environmental changes, therefore might be used as bioindicators of water quality. Rawa Jombor water conditions have changed as a result of the direct influence of the surrounding community activities or indirect effect such a change of land use. The purpose of this  research was to examine the composition, abundance, diversity, and evenness of phytoplankton in Rawa Jombor, assess the level of water pollution Rawa Jombor based on saprobic index and assess water quality Rawa Jombor by phytoplankton as a bioindicator. Determination of sampling points purposive random sampling. Sampling using plankton net no.25, and observation of the sample under the microscope 400 magnification. Temperature, pH, brightness,   turbidity   and   conductivity   is   still   relatively   good   conditions   for phytoplankton, while the value of the DO indicates the quality of waters classified as heavily polluted. The species composition of phytoplankton in Rawa Jombor are 21 types of 5 divisions (Bacillariophyte, Chlorophyte, Cyanophyte, Chrysophyte, and Euglenophyte), and is dominated by the division Cyanophyte. Species found in the entire       station  is          Anabaena             sp,          Anabaena          flos-aquae,           Anabaena     spiroides, Cylindrospermopsis raciborskii, Microcystis aeruginosa and Gonium pectorale. The highest abundance at STA 5 with 21,137 Ind/L and the lowest at STA 7 with 8,846Ind/L. Shannon-Wiener diversity index ranged from 0.84 to 1.47, evenness index ranged  from  0.35  to  0.59,  dominance  index  ranged  from  0.34  to  0.63,  and  the saprobic index ranges  between  (-2.48) - (- 2.87). Thoses value indicates that Rawa Jombor relatively heavy pollution. Keywords: Water quality, Community, Phytoplankton, Rawa Jombor.
Kadar Proksimat Telur Itik Pengging, Itik Tegal, Itik Magelang di Balai Pembibitan dan Budidaya Ternak Non Ruminansia (BPBTNR), Ambarawa Tika Andriani; Muhammad Anwar Djaelani; Tyas Rini Saraswati
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Local duck native germplasm Indonesia which has the genetic quality and potential to be developed as a prolific egg producer. Each type has a different chemical composition duck on her eggs. This study aims to transform and analyze proximate Pengging duck eggs, Tegal duck, and Magelang ducks that includes protein, fat, water, ash and carbohydrates. Egg samples were taken from three different types of ducks, namely: Magelang ducks, Tegal duck and Pengging duck six months old to be tested proksimatnya levels. This study consisted of 3 treatments and 6 repetitions on each type of duck eggs. Data were analyzed using analysis of variance (ANOVA), followed by a further test using the Least Significant Differences (LSD) at 95% confidence level. The results showed that levels of the protein and no significant water on three types of duck eggs, while the levels of fat, ash and carbohydrates showed significantly different results. It can be concluded that the difference in levels proximate duck eggs from Magelang ducks, Tegal ducks and Pengging ducks revealed that there is a difference of potential metabolites of the three types of ducks.Keywords: Duck Egg, Proximate, Least Significant Differences
Komposisi dan Kemelimpahan Fitoplankton di Telaga Menjer, Wonosobo. Dyah Ayu Kumalasari; Tri Retnaningsih Soeprobowati; Sapto Purnomo Putro
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Phytoplankton are the dominant primary producers in aquatic ecosystems, Phytoplankton are sensitive  to   environment  as  changes.  Therefore,  the  phytoplankton  is  often  used  for bioindicators of water quality.  The purpose of this study was to obtain information on the abundance, diversity, evenness, and dominance of phytoplankton in Menjer Lake and examine the condition of the water quality and its effects on phytoplankton abundance. Research on the composition and abundance of phytoplankton in Menjer Lake was conducted in May 2015. Phytoplankton samples were taken using a plankton net size 25 µk and identification of phytoplankton using SRC and the microscope. The composition of phytoplankton in Menjer Lake consist of  40 species from six divisions in the following order: Bacillariophyte (17 species),  Chlorophyte  (15   species),   Cyanophyte  (3  species),  Chrysophyte  (1  species), Chryptophyte (1 species  ) and  Dinoflagellate  (3 species).  Phytoplankton abundance was highest at the net culture (11.884 ind/ l), then the abundance of phytoplankton low at the outlet station (8.476 ind/ l). The result of analysis on Phytoplankton diversity of stasions at Menjer lake the value 2,27-2,62 that means of high diversity. Uniformity index (e) at Menjer lake the value 0,84-0,92, that means the condition of phytoplankton community include into stable. Dominance index  (D) was highest at the outlet station phytoplankton species to dominate Aulacoseira granulata was indicated of its water is eutrophic. Keywords: Menjer Lake, bioindicators, phytoplankton
Perbedaan Somatometri Itik Tegal, Itik Magelang Dan Itik Pengging Desi Wulandari; S Sunarno; Tyas Rini Saraswati
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Local ducks in Indonesia there are several kinds, for example Tegal ducks, Magelang ducks and Pengging duck each having characteristics different somatometri. Research on somatometri ducks that includes body weight, leg length, beak length, wing length, body length, and long-neck ducks important to know somatometri of each duck. This study aimed to analyze the differences somatometri at Tegal ducks, Magelang ducks and Pengging duck, which includes weight, leg length, beak length, wing length, body length, and a long neck. Ducks were used in this study maintained in the Central Breeding and Raising Ducks Work Unit Non Ruminant Livestock Banyubiru, Ambarawa. The study was conducted by measuring somatometri of each six monthold ducks. Duck samples taken from three different types of ducks that Tegal duck, Pengging duck and Magelang duck, of each type of sample taken many ducks as 6 tails. Data were analyzed using analysis of variance (ANOVA) with the differentiating factor is the type of duck. If the data were analyzed contained a real difference, then do a further test using the test Least Significant Differences (LSD) at 95% confidence level. The results showed that there was no significant difference in all parameters of the study. Conclusion of the study three types of ducks will have somatometri not unlike that includes, weight, leg length, beak length, wing length, body length and the length of the neck. It was because give feed and feed same time. Keywords: Local Ducks in Central Java, Somatometri, Tegal ducks, Magelang ducks, Pengging duck
Biodegradasi Senyawa Hidrokarbon Oleh Strain Bacillus cereus(VIC) Pada Kondisi Salinitas Yang Berbeda Reza Auliarahman Bhaktinagara; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacillus cereus has been noted as hydrocarbonoclastic microbe that has ability to degrade hydrocarbons in non-saline conditions and some often to be found on high salinity environtment conditions. The purpose of this research is to determine the ability of strains Bacillus cereus (VIC) were isolated from non-saline environment to degrade hydrocarbons in crude oil on the different salinity condition. Bacillus cereus (VIC) was inoculated on the medium that has contaminated by crude oil with salinity level of 0,3 , 9,4 , dan 19,6 and incubated for 15 days. Determination of microbial growth is by using Total Plate Count (TPC) method along with determination of Total Petroleum Hydrocarbon (TPH) percentage using gravimetry method for every 5 day. The determination of microbial growth showed that Bacillus cereus (VIC) has the tolerancefor salinity level up to 19,6 because it is able to grow to a density of 6.9 x 106 CFU/ml on the 15th day. Bacillus cereus (VIC) is also able to degrades hydrocarbons on crude oil pollutants that indicated from degradation of TPH percentage in the medium up to 21% during 15 days incubation period on the medium with salinity level of 19,6 . Biodegradation using Bacillus cereus (VIC) can increase TPH degradation on the medium up to 19,8% than TPH degradation because of weathering. Keywords: Biodegradation, Bacillus cereus(VIC), Total Petroleum Hydrocarbon (TPH), Salinity.
Morfoanatomi Polen Flora Mangrove Di Pantai Banjir Kanal Timur, Semarang Titi Nurul Qodriyyah; Sri Widodo Agung Suedy; Sri Haryanti
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Indonesia has the largest mangrove area in the world that covering an area of 2.5-5 million ha. Indonesia's vast mangrove area has been declined by 50%. Locations that becoming sampling stations, namely East Flood Canal (BKT) undergo abrasion that caused water-logging in mangrove forest areas that affect the diversity of plants in it. Plant diversity can be learned through the pollen of plants. Morphology-anatomy can be used as additional criteria for identifying plant species. The aim of this study was to determine the diversity of plants and morphology-anatomy pollen of plants on the coast BKT. Sampling was conducted using roaming at both locations. Data were analyzed by making the description and classification. The results showed that mangrove forests in BKT have a diversity of plants as many as 9 species. Morphology-anatomy pollen of different species have a variety of shapes and sizes. Keywords: mangrove, morphology-anatomy, pollen, Semarang

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