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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol. 6 No. 1 Januari 2017" : 11 Documents clear
KUALITAS MADU LOKAL DARI LIMA WILAYAH DI KABUPATEN WONOSOBO Rofiqotul Khasanah; Sarjana Parman; Sri Widodo Agung Suedy
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Honey is sweet natural substance produced by honey bees (Apis sp.) from the nectar or other parts of plant. The nutritional content influences its quality. The contents are sugar, then water, and also some of organic acids. The purpose of this research is to analyse the honey quality from its water content, acidity, and sugar content in local honey from Wonosobo Regency. The samples were taken in the five locations, i.e Topengan village (TP), Pringapus (PA), Kalikuning (KK), Krinjing (KJ), and Mutisari (MS). The data analysis with ANOVA test (α = 5%). The Standardisation's reference of honey quality based on Indonesia National Standardisation (SNI) 2013 and EC Directive in 2001 year. The Results were water content was 22.17 - 23.67%, acidity was 34.08 - 39.37 ml NaOH/ kg, and sugar content was 74.83% - 76.17%. All samples were in low quality, except PA that was in standard with water content was 22,17%, acidity was 34,08 ml NaOH/kg, and sugar content was 76,17oBx. Keywords: honey quality, water content, acidity, and sugar content
AKTIVITAS ANTAGONISTIK KAPANG ENDOFIT DUWET (Syzygium cumini (L.) Skeels) TERHADAP Alternaria porri PENYEBAB BERCAK UNGU PADA BAWANG MERAH (Allium ascalonicum L.) SECARA IN-VITRO Ristia Rachmatunnisa; MG. Isworo Rukmi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Endophytic fungi has been capable in producing secondary metabolites similar to those produced by its host. Secondary metabolites in some parts of duwet tree showed an antifungal activity. The aims of this study were to determine the ability of duwet endophytic fungi in inhibiting A. porri fungus, a pathogenic agent for  purple blotch disease on onion. The experiment conducted using CRD with eight endophytic fungi isolates as treatment with three replications. Antagonistic activity observed using dual culture method. The endophytic fungal isolates were conventionally identified to genus level. The results showed that endophytic fungi were identified as five isolates of Penicillium and one isolate of Aspergillus, Fusarium and mycelia sterilia. The antagonistic acitivity of duwet endophytic fungi against A. porri were varied between 18.1% - 47.3%. The highest antagonistic activity showed by Fusarium JD1 (47,3%). Keywords: Alternaria porri, purple blotch, onion disease, antagonistic fungi, endophytic fungi.
OPTIMASI PERTUMBUHAN DAN POTENSI ANTAGONISTIK Bacillus pumilus TERHADAP PATOGEN Xanthomonas campestris SERTA IDENTIFIKASI MOLEKULER GEN PENYANDI PKS DAN NRPS Laila Nur Faizah; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Xanthomonas campestris is one of the broccoli pathogen that difficult to be eradicated. B. pumilus is a prospective biocontrol agent due to its ability to produce antibiotical subtances that inhibit the growth of pathogenic bacteri and fungi. Beside that, B. pumilus have a great endurance to extreme environment by its ability to form spores. Some species of Bacillus genus have been reported to have PKS (Polyketide Synthetase) and NRPS (Non-Ribosomal Peptide Synthetase) coding gene which is a mega shyntetase that have a role in the production of antibiotic subtances. This reseach aims to test the inhibitory potency of B. pumilus toward X. campestris, identify the PKS and NRPS coding gene and to knwing the optimum growth of B. pumilus on the medium with different pH and carbon source. Reseach is conducted with Completely Randomized Design (CRD) with three replications on two growth parameters that is pH and carbon source. pH treatment is conducted with pH 5, 6, 7, and 8, while carbon source treatment is conducted with glucose, fructose, and sucrose as the carbon source. The obtained result is analized with ANOVA with significant level of 95%. The result shows B. pumilus ability to inhibits the growth of X. campestris producing inhibitory zone with 1,84 mm of diameter. The reseach showed negative result in NRPS coding gene detection and a positive result in PKS coding gene. PKS coding gene identification which is analized with BLAST shows 98% homology with PKS coding gene of Bacillus subtilis HNS005. Growth optimization shows the optimum groth of B. pumilus at pH 6 and glucose as the carbon source.Keywords: biocontrol, B. pumilus, PKS, NRPS, growth optimization
UJI ANTAGONIS KAPANG ENDOFIT DUWET (Syzigium cumini (L.) Skeels) TEHADAP KAPANG Fusarium oxysporum PENYEBAB PENYAKIT MOLER PADA BAWANG MERAH (Allium ascalonicum L.) SECARA IN-VITRO Luthfian Nur Afifi; MG. Isworo Rukmi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Endophytic fungi inhabit plant hosts for all or part of their life cycle in plant tissues without doing harm to its host. Utilization of endophytic fungi as controlling plant pathogens has been widely studied in in-vivo and in-vitro. The aims of this study was to determined the antagonistic activities of 8 endophytic fungal isolates from some parts of duwet (Syzigium cumini (L.) Skeels.) tree against F. oxysporum, a pathogenic fungi causing bulb rot disease on red onion. This study was conducted using Completely Randomized Design (CRD), with 8 endophytic fungal isolates as treatment, done in triplicates. The antagonistic activity were examined using dual culture method, by determined the percentage of inhibition. The mycelial plugs (4 mm diameter) of endophytic fungi and pathogenic fungi F. oxysporum were placed in same dish 3 cm each other. The endophytic fungal isolates were conventionally identified to genus level. The identification results showed that endophytic fungi came from 1 isolates of Aspergillus, 5 isolates of Penicillium, 1 isolate of Fusarium, and 1 isolate of Mycelia sterilia. All endophytic fungal isolates showed capability on inhabiting the growth of F. oxysporum with the percentage of inhibition varied between 1.1 to 36.0%. Fusarium JD1 isolates showed the highest antagonist activity against F. oxysporum (36.0%). Keywords : Endophytic fungi, duwet, Fusarium oxysporum, growth inhibition.
KEANEKARAGAMAN JENIS ANGGOTA LAURACEAE DAN PEMANFAATANNYA DI CAGAR ALAM DUNGUS IWUL KABUPATEN BOGOR JAWA BARAT Seviana Mulia; M Murningsih; J Jumari; Lade Alhamd
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lauraceae is a group of flower plant that have a significant economic value. In the Natural Preserve of Dungus Iwul has been identified the existence of the Lauraceae family. The purpose of this research is to determine the diversity of Lauraceae species, understand Lauraceae density and frequency in Natural Preserve of Dungus Iwul, and understanding it’s utilization. The observation was done on 1 ha plot sample, which include identification, inventarization, and the collection of the Lauraceae species, the research was done on May until June 2016. There are 48 individual members of the Lauraceae species which is consist of nine species and 4 genera, e.g. Cryptocarya, Litsea, Actinodaphne, Cinnamomum. The highest density value were Litsea racemosa C.T White with 29,79% as relative density and the lowest density were Cinnamomum javanicum Blume and Litsea resinosa Blume with 2,13% as relative density. The species with the highest frequency were Litsea racemosa C.T White with 24,32% as relative frequency value and the species with the lowest frequency were Cinnamomum javanicum Blume dan Litsea resinosa Blume with 2,70% as relative frequency value. The Lauraceae family have benefits such as animal feed, building materials, cover crops, ornamental plants, industrial raw materials, essential oil, and act as a medicine.Keywords :     Lauraceae, Natural Preserve of DungusIwul, species diversity,                                             density and frequency plants utilization.
PENGARUH LAMA PENYIMPANAN TERHADAP PERUBAHAN MORFOLOGI DAN KANDUNGAN GIZI PADA UMBI TALAS BOGOR (Colocasia esculenta (L.) Schott) Jean Cafriany Suryana Putri; Sri Haryanti; Munifatul Izzati
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Storage of agricultural products is an important thing to do in post-harvest handling. Bogor Taro (Colocasia esculenta (L.) Schott) is a perishable agricultural products at harvest time, so it takes a good handling to be able to maintain its quality as a functional food ingredient (instead of rice). This study aims to determine the long effect of storage on morphology changes, proximate and vitamin B1 content of the tuber Bogor taro. The study design used Completely Randomized Design (CRD) for storage time of 5 days (K.I), 10 days (K.II) and 15 days (K.III) with three replications. The main parameters of the study are the morphological changes (weight loss, shrinkage diameter, tuber damage, the presence of buds) and the chemical changes (proximate and vitamin B1 content). The parameters also play a role in the form of environmental storage conditions (temperature, humidity and light intensity). The analysis of the data was done using Analysis of Variance (ANOVA) followed by further test of Duncan’s Multiple Range Test (DMRT) at the level of 95%. The result showed the long effect of different storage significantly affect weight loss, but didn’t significantly affect shrinkage diameter and cause tuber damage and growth of shoots. The long effect of different storage significantly affect water content and vitamin B1, but didn’t significantly affect the ash content, crude lipid content, crude protein content and crude fiber content. The duration of storage for 5 days (K.I) showed the best results in maintaining their nutritional value. Keywords: Bogor taro (Colocasia esculenta (L.) Schott), storage, proximate vitamin B1
PERTUMBUHAN SEMAI RHIZOPHORA MUCRONATA PADA LUAS SALURAN TAMBAK WANAMINA YANG BERBEDA Hadyani M Hanifa; Endah Dwi Hastuti; Rini Budi Hastuti
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The mangrove forest is a natural resource that is vital to the life of coastal communities, and has experienced the threat of relegation very fast. Seedling growth is important in mangrove conservation in wanamina (silvofishery). This study aimed to examine the growth of mangrove seedlings R. mucronata on different wide of channel ponds. Planting mangrove seedlings carried on channel ponds wanamina (Silvofishery) with a 5 m of length and varies width, that is 1 m; 2 m and 3 m. This research used experimental method with Randomized Design (RBD) conducted for 4 months with the observation period once every two weeks. Mangrove growth was observed are the number of leaves, number of branches, seedling height and stem diameter. Data analysis was performed using Analysis of Variance (ANOVA). The results showed that there was a tendency of better seedling growth in the outlet channel and the channel with 1 m width.  Key words: growth, mangrove, silvofishery, seedlings.
PRODUKSI SELULASE OLEH KAPANG Aspergillus sp. HASIL ISOLASI DARI LIMBAH PENGOLAHAN SAGU (Metroxylon sp.) DENGAN VARIASI KONSENTRASI INOKULUM PADA FERMENTASI TERENDAM STATIS Anisa Rachma Sari; Endang Kusdiyantini; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sago processing waste is one of agro-industry waste, which contains cellulose 19,55% and lignin 25%. Genus Aspergillus has been widely known as cellulase-producing fungi. Isolation of Aspergillus from sago processing waste is expected to get a potential isolates indegenous cellulolytic fungi. Cellulase (EC 3.2.1.4) is an enzyme that can break down cellulose into glucose monomer form, with consists of three components that work in synergy are endoglucanase, eksoglucanase and β-glucoside. This study aims to obtain isolates of Aspergillus sp. cellulolitic potential from sago processing waste and cellulase production of the cellulolytic isolates in various concentrations of inoculum. The study was conducted using completely randomized design (CRD) 1 factor of 4 levels of inoculum concentration treatments were 0%, 2,5%; 5%; and 10% with three replications. The observed variables are biomass, CMCase activity and FPase activity. Data were analyzed by ANOVA with a significance level 95%. The result of isolation, two isolates of Aspergillus namely A. flavus and A. paradoxus with cellulolitic index respectively 2,63 and 2,06. Cellulase activity isolate of A. flavus either endoglucanase (CMCase) or total cellulase (FPase) are not affected by the concentration of inoculum.Keywords: cellulase, sago waste, Aspergillus, inoculum
ISOLASI DAN IDENTIFIKASI BAKTERI GENUS Sphingomonas DARI DAUN PADI (Oryza sativa) DI AREA PERSAWAHAN CIBINONG Gabriela Christy Sabbathini; Sri Pujiyanto; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The unique ability of  the genus Sphingomonas bacteria as degrade the contaminants refractory contaminants, to serve as the antagonists bacteria to phytopathogenic fungi, and capable to secrete  hidhly useful exopolysaccharide gellan make these bacteria may play an important role in various industrial fields. Exploitation of the metabolic capabilities by genus Sphingomonas bacteria can provide significant commercial advantages for biotechnology.The species of Sphingomonas are often found associated with the rice plant as one of the endophytic bacteria that can be cultured. This study aims to isolate the local bacteria that can produce gellan gum from the leaves of the rice plant (Oryza sativa). The isolation process is done with a spread plate method suspension of rice leaves on Nutrient Dextrose Agar (NDA) media. Single colonies of bacteria that can be isolated then identified by colony PCR method to proceed at sequencing process. Sequencing followed by equalization sequences on the BLAST program shows four isolates of the genus Sphingomonas which isolates XA1, XA2, XA6, XA12 with the results are Sphingomonas sp. Fse41, Sphingomonas sp. Fse41, Sphingomonas sanguinis L4-317 strain and Sphingomonas sp. MLB01Keywords: endophytic bacteria, padi, Sphingomonas
IDENTIFIKASI MOLEKULER TANAMAN PISANG RAJALAWE BERDASARKAN GEN INTERNAL TRANCRIBED SPACER (ITS) Firly Putri Fardilla; Hermin Pancasakti Kusumaningrum; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Banana is one type of horticultural commodities in a group of fruits that have a socio-economic value is high enough for the people of Indonesia. Bananas have different varieties, one banana type Rajalawe found in Central Java. Rajalawe molecular identification has not been done before. This study aims to determine the result of the identification of the molecular basis of  Rajalawe based on genes Internal transcribed spacer (ITS), in search of identity and kinship Rajalawe. The study was conducted by isolating DNA using a method Rajalawe Doyle & Doyle, followed by ITS gene amplification and sequencing analysis. The results of gene amplification ITS produce PCR product of 643 bp. The base sequence of the sequencing results are used for the construction of phylogenetic trees. Sequence similarity analysis Rajalawe show 95% homology with Musa balbisiana and alkaline difference of 1%. Phylogenetic tree analysis showed Rajalawe have a close relationship with Musa balbisiana. However, bananas Rajalawe has several different characters with Musa balbisiana with different base sequences by 5% whereas the base sequence homology between the banana Musa balbisiana and Rajalawe with 95%.Keywords: Molecular Identification, Pisang Rajalawe, Universal Primer ITS, Musa balbisiana.

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