cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Eko Didik Widianto
Contact Email
rumah.jurnal@live.undip.ac.id
Phone
+6281390576830
Journal Mail Official
jurnalbiologi@live.undip.ac.id
Editorial Address
Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang
Location
Kota semarang,
Jawa tengah
INDONESIA
Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 234 Documents
 14   15   16   17   18 
IDENTIFIKASI ISOLAT MONASCUS SP. HASIL ISOLASI ANGKAK BERDASARKAN GEN INTERNAL TRANSCRIBED SPACER (ITS) DAN PENGUKURAN KANDUNGAN PIGMEN Mia Tri Wardani; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.786 KB)

Abstract

The Identification of Monascus based on their morphological characteristic, this methods is very problematic due to several  specimens share similarity on morphology and colours. Nowadays, the identification of molecular based organisms has been done to complement morphological identification, for example mold identification using the Internal Transcribed Spacer (ITS) gene. The objectives of study were to identify of Monascus isolates the result of angkak isolation using Internal Transcribed Spacer (ITS) gene, phylogenetic analyzing,  determination cell growth and determination of production pigment content. Sequences of  ITS Monascus were amplified using PCR and the molecular phylogenetic  analyses was using Neighbor Joining (NJ) method. Based on homology search by Basic Local Alignment Search Tool (BLAST) program and phylogenetic tree analyses, mold of isolates were identified as the Monascus purpureus (100%). The amplified DNA fragments were about 553 bp. Determination of Monascus mold cell growth were carried out by the method of dry weight cell. Determination of pigment by using spectrophotometer at 390 nm wavelength for yellow pigment and 500 nm for red pigment. The result of determination of Monascus mold pigment content show that have increased during fermentation time until day 13. The amount of yellow extracellular pigment production is 37.358 U / g; While the red color pigment of 2.6545 U / g. The amount of red intracellular pigment production is 7.4175 U / g, while the yellow pigment is 30.176 U / g.Keywords: Monascus, angkak, molecular phylogenetic, pigment
Pertumbuhan Mikroalga Botryococcus braunii Sebagai Penghasil Lipid Pada Medium Campuran Antara Air Kelapa Dan Air Laut Bintoro Rudi Saputro; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.612 KB)

Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions  for bioenergy.  Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be subtituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of  P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using haemocytometer. The results showed that 2.5% coconut water subtitution exhibited the best growth rate and lipid production, ie the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control. Keywords: Growth, Botryococcus braunii, Lipid, Coconut water, Seawater.
AKTIVITAS ENZYM SELULASE YANG DIHASILKAN OLEH BAKTERI Serratia marcescens PADA SUBSTRAT JERAMI Khrisna Lazuardi Budi; W Wijanarka; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.554 KB)

Abstract

Cellulose (EC 3.2.1.4) is enzyme complex consisting of some enzymes which together decomposing cellulose into glucose by hydrolizes the β-1,4 bond in cellulose. The purpose of this study is to determine cellulose activity which produced by Serratia marcescens in different substrate concentration and at the time of incubation T4, T8, T12. This research uses Randomized Block Design (RBD) factorial pattern with two factors. The first factor was variation of straw substrate which had been delignificated (V0, V1, V2, V3). The second factor is the variation of time incubation (T4, T8, T12). Each factor is repeated 3 times. The data obtained were analyzed using Analysis  of  Variance (ANOVA)  (α  =  0.05). The result  shown that  variation concentration  of straw,  and the interaction (combination) between the straw substrate and the incubation time substrate was not significantly different. The result treatment of incubation time was significantly different of the cellulase activity. The result of anova analyzed is obtained that F count(α = 0.05) value from straw substrate, interaction (combination) between the straw substrate and the incubation time substrate, and incubation time was 0.53; 2.18; 8.00. F table(α = 0.05) value of straw substrate, interaction (combination) between the straw substrate and the incubation time substrate, and incubation time was 2.99; 2.20; 3.39. The result of anova, is continued by BNT 5% test. The result of BNT test shown that the highest incubation time of cellulase activity was in incubation time 12 hours with the average value 0.26 U/mL. Key Word : cellulose,  Serratia marcescens,  straw substrate, incubation time
ISOLASI DAN KARAKTERISASI ISOLAT KONSORSIUM BAKTERI LAHAN PERTANIAN SEBAGAI POTENSI DEGRADASI PESTISIDA PROPOXUR Ahmad Qi Sahlan; Endang Kusdiyantini; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.545 KB)

Abstract

Propoxur pesticide is a pesticide used widely enough by the Indonesian agricultural community. This pesticide is used as a systemic pesticide in agricultural areas to deal with pests as well as insecticides, herbicides, and bactericide. Study of the degradation of propoxur has long performed using the bacterial bioremediation agent. Bacterial consortium is a combination of several bacteria in a medium which that has a complementary function in the environment. The purpose of this study was the isolation and characterization of a consortium of farms that use propoxur pesticide. Isolation was done by spread plate and was observed characteristics of colony morphology. The obtained isolates were tested against propoxur and its potential role in the consortium. Degradation of propoxur observations using High Performance Liquid Cromatography (HPLC). Isolation results obtained 8 (eight) bacterial isolates and one that have an important role in the consortium is K1 isolate. K1 isolate were able to work up a consortium to degrade Propoxur in 2 isolates with K2 isolate. The interaction of these bacteria are natural and have a tendency to vary in nature after a process of isolation. K1 isolate are not able to work alone in a mineral medium, and worked with synergistic and complementary that in the bacterial consortium. KSR consortium optimum growth occurs in an environment with temperature 35 °C and pH 6.Keywords: Isolation, Propoxur, Bioremediation, Bacterial Consortium, HPLC
IDENTIFIKASI KANDUNGAN SENYAWA FITOKIMIA MINYAK BIJI MIMBA (Azadirachta Indica, A. Juss) Dyah Palupi; Endang Kusdiyantini; Rully Rahadian; A Heru Prianto
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.397 KB)

Abstract

Neem seeds oil (Azadirachta indica, A. Juss) have phytochemical compounds which act as antifeedant, repellent, antibacterial, and antifungal. The neem seeds oil were important identified their phytochemical compounds. Identifications were done with screening phytochemical and infrared spectrophotometer. Neem seed oils were obatained with pressing method. The group of compounds tested were alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. The results of phytochemical showed that neem seeds oil consist of alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. This results were strengthned with spectrum in infrared spectrophotometer that showed the functional groups of each those compounds. Keywords: neem seeds oil, phytochemical compounds, pressing method, functional groups
LAJU PERTUMBUHAN PUYUH (Coturnix coturnix japonica) SETELAH PEMBERIAN TEPUNG KUNYIT (Curcuma longa) PADA PAKAN Sylvia Frida Jamelah; Koen Praseno; Tyas Rini Saraswati
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.703 KB)

Abstract

Research on The Growth Rate of The Japanese Quail (Coturnix coturnix japonica) After the Addition of Turmeric Powder (Curcuma longa) on Feed the animal test subjects were the Japanese quails (Coturnix coturnix japonica) which grouped into several treatment groups i.e. P0 (group of quails fed with standard feeding), P1 (group of quails fed with standard feeding which then added with turmeric powder 54 mg/head/day on age 14 to 44 days old), and P2 (group of quails fed with standard feedingwhich then added with turmeric powder 54 mg/head/day on age 14 to 120 days old), while parameters measurement was carried out for 4 months. The main observed parameter was weight increment which measured every once a week starting from 7 days old quails in each treatment group, while proponent parameters were feed and water intake. Measurement of weight was done using scaleon each quail in battery cages. The growth rate was obtained by calculating the difference between last week weight with first week weight per unit time, and was done on each quail in battery cages. Obtained data was analyzed using Analysis of variance (ANOVA) on the basis of a completely randomized design (CRD). If there was a difference among the treatments then proceed with further testing, using Duncan Test at a significance level of 95%. Based on the analysis of variance, it showed that there was a significant difference on feed intake while the growth rate didn’t indicate significant difference. Based on these results, it can be concluded that the turmeric powder as feed additive has no potential to increase the weight and the growth rate of the Japanese quail. Key words: Growth Rate, Quail (Coturnix coturnix japonica), turmeric powder (Curcuma domestica)
ISOLASI DAN IDENTIFIKASI BAKTERI GENUS Sphingomonas DARI DAUN PADI (Oryza sativa) DI AREA PERSAWAHAN CIBINONG Gabriela Christy Sabbathini; Sri Pujiyanto; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.972 KB)

Abstract

The unique ability of  the genus Sphingomonas bacteria as degrade the contaminants refractory contaminants, to serve as the antagonists bacteria to phytopathogenic fungi, and capable to secrete  hidhly useful exopolysaccharide gellan make these bacteria may play an important role in various industrial fields. Exploitation of the metabolic capabilities by genus Sphingomonas bacteria can provide significant commercial advantages for biotechnology.The species of Sphingomonas are often found associated with the rice plant as one of the endophytic bacteria that can be cultured. This study aims to isolate the local bacteria that can produce gellan gum from the leaves of the rice plant (Oryza sativa). The isolation process is done with a spread plate method suspension of rice leaves on Nutrient Dextrose Agar (NDA) media. Single colonies of bacteria that can be isolated then identified by colony PCR method to proceed at sequencing process. Sequencing followed by equalization sequences on the BLAST program shows four isolates of the genus Sphingomonas which isolates XA1, XA2, XA6, XA12 with the results are Sphingomonas sp. Fse41, Sphingomonas sp. Fse41, Sphingomonas sanguinis L4-317 strain and Sphingomonas sp. MLB01Keywords: endophytic bacteria, padi, Sphingomonas
Perbedaan Somatometri Itik Tegal, Itik Magelang Dan Itik Pengging Desi Wulandari; S Sunarno; Tyas Rini Saraswati
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.356 KB)

Abstract

Local ducks in Indonesia there are several kinds, for example Tegal ducks, Magelang ducks and Pengging duck each having characteristics different somatometri. Research on somatometri ducks that includes body weight, leg length, beak length, wing length, body length, and long-neck ducks important to know somatometri of each duck. This study aimed to analyze the differences somatometri at Tegal ducks, Magelang ducks and Pengging duck, which includes weight, leg length, beak length, wing length, body length, and a long neck. Ducks were used in this study maintained in the Central Breeding and Raising Ducks Work Unit Non Ruminant Livestock Banyubiru, Ambarawa. The study was conducted by measuring somatometri of each six monthold ducks. Duck samples taken from three different types of ducks that Tegal duck, Pengging duck and Magelang duck, of each type of sample taken many ducks as 6 tails. Data were analyzed using analysis of variance (ANOVA) with the differentiating factor is the type of duck. If the data were analyzed contained a real difference, then do a further test using the test Least Significant Differences (LSD) at 95% confidence level. The results showed that there was no significant difference in all parameters of the study. Conclusion of the study three types of ducks will have somatometri not unlike that includes, weight, leg length, beak length, wing length, body length and the length of the neck. It was because give feed and feed same time. Keywords: Local Ducks in Central Java, Somatometri, Tegal ducks, Magelang ducks, Pengging duck
PRODUKSI ENZIM INULINASE Pichia manshurica DUCC-Y015 DENGAN PENAMBAHAN SUBSTRAT TEPUNG BENGKOANG (Paschyrhizus erosus) Adzar Rofiqoh; W Wijanarka; Susiana Purwantisari
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.657 KB)

Abstract

Bengkoang (Pachyrhizus erosus) tubers has a high inulin content. Inulin bengkoang flour can be used as substrate to produce inulinase enzyme. The inulinase enzyme can be produced by Pichia manshurica DUCC-Y015. This research aims to determine the ability of Pichia mansurica DUCC-Y015 in producing inulinase enzyme with the addition of several variations of substrate concentration of bengkoang flour in its production medium. Determination of inulinase activity was done by DNS method. This research used a Completely Randomized Design (RAL) with 4 treatments: B0 (control), B1 (1 g bengkoang flour), B2 (3 g bengkoang flour) and B3 (5 g bengkoang flour). Each treatment was repeated 3 times. The inulinase activity of each treatment was 0.029 IU/mL, 0.033 IU/mL, 0.053 IU/mL and 0.015 IU/mL. The addition of variation substrate concentration bengkoang flour in the production medium did not affect the inulinase activity of Pichia manshurica DUCC-Y015Kata Kunci: Pachyrhizus erosus, inulinase, Pichia manshurica DUCC-Y015.
EKSPLORASI RHIZOBAKTERI INDIGENOUS TANAMAN CABAI RAWIT (Capsicum frustescens Linn.) DARI PERTANIAN SEMI ORGANIK DESA BATUR KABUPATEN SEMARANG SEBAGAI AGEN HAYATI PENGENDALI PERTUMBUHAN JAMUR Fusarium oxysporum f.sp capsici M Eka Prastya; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.277 KB)

Abstract

Rhizobacteria is a group of bacteria that live in the around of plant roots. This type of bacteria known have the ability to stimulate plant growth by producing growth hormone, as well as to inhibit the growth of plant pathogens synthesize compounds with antibiotics or extracellular enzymes. The purpose of this study was to obtain and describe the morphological, biochemical and genetic isolates rhizobacteria of semi-organic farmland Semarang District village Batur which has the ability as a biological control agent of fungal pathogens Fusarium oxysporum f.sp capsici. The results obtained fifteen isolates the majority rhizobacteria bacilus shaped gram-positive and classified. Rhizobacteria inhibition test capabilities against pathogenic fungi was performed using dual culture test and test biomass. Dual culture test results showed that the inhibition of isolates E1 has a 3.77%, 1.88% isolates E3 and E15 isolates 22%. The biomass tests show E15 isolates capable of inhibiting the growth of pathogenic fungi best with the smallest weight of fungal biomass 0.0386 grams. The results of the molecular characterization based on 16S rRNA gene sequences to known that E15 isolates has similar with Bacillus cereus strain ATCC 14579 with similarity of 97%. The results of the biochemical characterization of isolates E15 has similarities with B. cereus species that is catalase positive, motile, have endospores, is able to hydrolyze starch and ferment glucose.Keywords: rhizobacteria, Fusarium oxysporum f.sp capsici, gen 16S rRNA, Bacillus cereus

Page 16 of 24 | Total Record : 234

 14   15   16   17   18 

Filter by Year

2012 2021


Reset
Filter By Issues
All Issue Vol. 10 No. 1 Januari 2021 Vol. 9 No. 2 Juli 2020 Vol. 9 No. 1 Januari 2020 Vol. 8 No. 2 Juli 2019 Vol. 8 No. 1 Januari 2019 Vol. 7 No. 4 Oktober 2018 Vol. 7 No.3 Juli 2018 Vol. 7 No.2 April 2018 Vol. 7 No. 1 Januari 2018 Vol. 6 No. 4 Oktober 2017 Vol. 6 No. 3 Juli 2017 Vol. 6 No. 2 April 2017 Vol. 6 No. 1 Januari 2017 Vol. 5 No. 4 Oktober 2016 Vol. 5 No. 3 Juli 2016 Vol. 5 No. 2 April 2016 Vol. 5 No. 1 Januari 2016 Vol. 4 No. 4 Oktober 2015 Vol. 4 No. 3 Juli 2015 Vol. 4 No. 2 April 2015 Vol. 4 No. 1 Januari 2015 Vol. 3 No. 4 Oktober 2014 Vol. 3 No. 3 Juli 2014 Vol. 3 No. 2 April 2014 Vol. 3 No. 1 Januari 2014 Vol. 2 No. 4 Oktober 2013 Vol. 2 No. 3 Juli 2013 Vol. 2 No. 2 April 2013 Vol. 2 No. 1 Januari 2013 Vol. 1 No. 1 Oktober 2012 More Issue