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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 234 Documents
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Uji Potensi Ekstrak Daun Suren dalam Menghambat Pertumbuhan Jamur Colletotrichum Capcisi secara In Vitro Fiva Andriyani; Susiana Purwantisari
Jurnal Akademika Biologi Vol. 8 No. 1 Januari 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun suren terhadap penghambatan pertumbuhan jamur patogen Colletotrichum capsici. Penelitian dilakukan di Laboratorium Pengamatan Hama dan Penyakit Tanaman (PHP) Kedu Temanggung dan Laboratorium Terpadu Universitas Diponegoro, Semarang. Ekstrak daun suren dengan konsentrasi 0%, 10%, 20%, dan 30% diujikan terhadap pertumbuhan jamur C.capsici secara in vitro. Pengamatan penghambatan pertumbuhan jamur C.capsici dilakukan pada hari ke-5, ke-10, dan ke-15 setelah inokulasi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan setiap perlakuan masing-masing dilakukan tiga pengulangan. Data yang diperoleh ke dianalisis dengan ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun suren mampu menghambat pertumbuhan jamur C.capsici. Ekstrak daun suren dengan konsentrasi 30% menunjukkan penghambatan tertinggi dibandingkan dengan konsentrasi yang lain terhadap pertumbuhan jamur C. capsici. 
PEMBERIAN KOMBINASI PUPUK DAUN GANDASIL D DENGAN PUPUK NANO-SILIKA TERHADAP PERTUMBUHAN BIBIT MANGROVE (Bruguiera gymnorrhiza) Widi Hastuti; Erma Prihastanti; Sri Haryanti; Agus Subagyo
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Increased damage to mangrove forests are increasingly threatened mangrove making function is reduced. Sustainability of mangrove forest areas depend on seedlings. Mangrove Nutrition is important, especially during the breeding. The addition of fertilizer leaves by spraying the leaves can maximize fertilizer efficiency. Dry environmental conditions and high stress on the lives of mangroves to make silica used in improving the sustainability of mangrove. This research aim to analyze the effect of the combination of Gandasil D leaf fertilizer and nanosilica fertilizer and analyze the optimal concentration of combination Gandasil D leaf fertilizer and nanosilica fertilizer  in mangrove plant seeds (Bruguiera gymnorrhiza) growth. Research conducted with Complete Random Design with one factor. There are 6 treatments: control, leaf fertilizer 75% + 25% Nanosilica, leaf fertilizer 50% + 50%, leaf fertilizer 25% + 75% Nanosilica, Nanosilica 100%, leaf fertilizer 100%. The experiments  was conducted with 5 replicates. The parameters observed, amount of leaves, leaf area, high of plants, amount of chlorophyll and leaf color. Supporting parameters include temperature, pH, moisture and air. Analysis of the data used is ANOVA followed by further test of Duncan Multiple Range Test (DMRT) at the 95% significance level. The results indicate that the combination of leaf fertilizer and Nanosilica effect on growth (Bruguiera gymnorrhiza). The combination of optimum fertilization in increasing growth that is 50% leaf fertilizer and 50% Nanosilica. Keywords: mangrove, Bruguiera gymnorrhiza,  Gandasil D leaf fertilizer, nanosilika fertilizer
PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN SORGUM (Sorghum bicolor L. Moench) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Annisaa Widyasari; W Wijanarka; Budi Raharjo; Mamik Setyowati
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sorghum was a cereal crop that has many benefit such as food, feed, industrial, and bioenergy. Sorghum had a potency to be cultivated, but productivity of sorghum was still low both in quatity and quality. One way to increase production of sorghum  is using rhizobacteria as biofertilizer. The aim of this study is to get rhizobacteria that has the ability to produce IAA, solubility of phospat (P), Nitrogen (N) fixing, and analyze the effect of rhizobacteria inoculants for enhance sorgum plant growth. Isolation of rhizobacteria was done by diluting  rhizobacteria sorghum suspension from 10-1 to 10-5 and it were be platted on SEA medium. Isolates were screened by ability to produce IAA, solubility of P, and N fixing. Producing of IAA test was done by adding Salkowsky reagent on bacterial supernatant and measured absorbance at 530 nm wavelength. Solubility of P test was done by inoculating isolates in Pikovskaya media, while N fixing test was done on N fixing media (NFB). Isolates of rhizobacteria which had a potency to increase growth of plants were made inoculants to be applied in sorghum plants. The result of this study obtain 3 isolates i.e Sr 194.3; Sr 172.1; and Sr 209.1 which were considered effective for increase growth of sorghum. The conclusion  of this study isolates which showed the highest average plant height, root length, and dry weight Sr 194.3 isolate. The statistical analysis among the treatments showed that did not any significant differences on plant height, root length, and dry weight of sorghum age 28 days after farming. Keyword : Increase growth plants, Screening, Shorgum, Rhizophere.
Indeks Kuning Telur (IKT), Haugh Unit (HU) dan Bobot Telur pada Berbagai Itik Lokal di Jawa Tengah Dwi Purwati; Muhammad Anwar Djaelani; Enny Yusuf Wachidah Yuniwarti
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Ducks is one of poultry producer eggs that potential in Indonesia. Research about ducks eggs quality which includes egg yolk index (IKT), haugh unit (HU) and eggs weight local ducks in central java very important to know the genetic ability to ducks from each region and as an attempt to provide and improve the quality of  foodstuffs. The purpose of the research was analyze eggs quality from local breeds in Central Java that is Tegal ducks, Magelang ducks and Pengging ducks, viewed from the value IKT, HU and weight eggs. Ducks used in this research are raised in the hall breeding and raising ducks satker non ruminant Banyubiru, Ambarawa. The research was done by measuring egg quality from each ducks that was six month. Data  were  analyzed using analysis of variance (ANOVA) with the differentiating factor is the type of ducks. If the data were analyzed significantly different, then do a further test using the Least Significant Differences Test (LSDt) at 95% confidence level. The results showed that ducks eggs Magelang has the heaviest weight  copared with Tegal ducks and Pengging ducks. Eggs quality data include IKT and HU showed no  significantly different from each ducks. The research conclusion the eggs Magelang ducks is the best eggs.   Keywords: Local Ducks Cental Java, Eggs Quality, IKT, HU, Egg Weight
PERTUMBUHAN DAN PRODUKSI PIGMEN MERAH OLEH Serratia marcescens PADA BERBAGAI SUMBER KARBON Setiawan Wicaksono; Endang Kusdiyantini; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Serratia marcescens is one of the red pigment producing bacteria which is widely used as natural dye. This bacterium was isolated from the sediment of hot springs in Gedong Songo, Bandungan, Semarang. S. marcescens has potential as a natural pigment producer. This study was conducted to measure the growth and production of pigments in NB medium containing different carbon sources. The sources of carbon used were glucose, fructose, maltose, and lactose. The method used were growth measurement based on dry weight value of cell and value of ODλ=600nm, measurement of reducing sugar, measurement of the acidity of the growth medium, and measurement of red pigment concentration. The results obtained in this study indicated that the provision of carbon sources has no significant effect on the growth of S. marcescens. The best carbon source for red pigment production is lactose with pigment concentration of 0.451 mg/L achieved at 24 hours incubation time.Keywords: Serratia marcescens, Growth, Carbon Source, Red Pigment.
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI RUSIP IKAN TERI (Stolephorus sp.) Deverina Mergypta; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rusip is a traditional food from Bangka-Belitung made from fresh anchovy with salt and palm sugar that is brooded for seven days. Rusip fermentation process is caused by microbial activity mainly from lactic acid bacteria. The purpose of this study was to isolate lactic acid bacteria from rusip, to characterize isolates obtained based on common characteristic of lactic acid bacteria, and to carry out proximate analysis of rusip anchovy. Isolation from rusip fermented food obtained eight bacterial isolates, five isolates were circular and three isolates were rod-shaped. Characterization and identification of lactic acid bacterial were performed using tests that led to the common characteristic of lactic acid bacteria, i.e the gram staining, motility test, acid formation test, catalase test, and proteolytic test. The result showed that eight bacterial isolates were gram-positive, non-motile, catalase-negative, positive to produce acid, and had proteolytic activity. Rusip anchovy had 0,64% of total lactic acid and the pH 5,40. Nutrient content of rusip fermentation had 10,3644% moisture content; 50,0702% ash content; 0,9524% crude fat content; 0,3764% crude fiber content; 34,8603% crude protein content; and 14,1171% carbohydrate content. Keywords: rusip, isolation, characterization, lactic acid bacteria, proximate analysis
Pengaruh Penambahan Tepung Azolla Sp. dan Perbedaan Waktu Inkubasi Terhadap Produksi Inulinase Khamir Pichia manshurica DUCC Y-015 Pada Medium Tepung Umbi Dahlia (Dahlia Variabilis Willd.) Rahmawati Dewi; Wijanarka Wijanarka; Sri Pujiyanto
Jurnal Akademika Biologi Vol. 7 No. 4 Oktober 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulin merupakan polimer fruktosa berserat pangan tinggi yang salah satunya ditemukan pada umbi dahlia yang disekitar perakarannya  ditemukan Pichia manshurica. P. manshurica menghasilkan enzim inulinase yang dapat memecah inulin menjadi uni-unit fruktosa dan digunakan sebagai bahan bahan baku pembuatan sirup fruktosa. Berdasarkan manfaat yang dimiliki inulin tersebut, telah banyak dilakukan penelitian mengenai optimasi produksi enzim inulinase oleh P. manshurica DUCC Y-015 untuk mendapatkan produk yang optimal. Optimasi produksi enzim inulinase dapat dilakukan dengan memodifikasi kandungan nutrisi dalam medium, salah satunya dengan menambahkan sumber nitrogen pada medium produksi. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan tepung Azolla sp. sebagai sumber nitrogen dan perbedaan waktu inkubasi. Penelitian dilakukan secara eksperimental menggunakan Rancangan Acak Kelompok pola factorial, dengan tiga kali ulangan. Faktor pertama adalah variasi konsentrasi tepung Azolla sp., yaitu 0%; 0,14%; 0,28%; dan 0,42%. Faktor kedua adalah variasi waktu inkubasi yaitu 0 jam; 6 jam; 12 jam; 18 jam; dan 24 jam.  Hasil perhitungan Anova menunjukkan bahwa penambahan berbagai konsentrasi Azolla sp. pada medium produksi belum mempengaruhi aktivitas inulinase dan invertase P. manshurica DUCC Y-015, sedangkan waktu inkubasi berpengaruh nyata terhadap aktivitas inulinase dan  aktivitas invertase. Berdasarkan uji BNT, waktu inkubasi optimal aktivitas inulinase dan invertase adalah pada waktu inkubasi 6 jam. 
PERTUMBUHAN SEMAI R. mucronata PADA TAMBAK WANAMINA DENGAN LEBAR SALURAN INLET DAN KOMPOSISI MANGROVE YANG BERBEDA Ruli Nur Hidayanti; Rini Budi Hastuti; Endah Dwi Hastuti
Jurnal Akademika Biologi Vol. 5 No. 1 Januari 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Fishpond as aqua culture in certain regions are managed intensively to obtain optimum results. Rising the areas of fishpond induced decrease of mangroves areas due to land conversion. Managing sytem of fishpond in association with mangrove forests began to be developed and known as silvofishery.         R. mucronata is one type of mangrove which has an important role for the environment of fishpond. This research aims to know the growth of R. mucronata in duct inlet of silvofishery with different width and mangrove compositions. This research was carried out axperimentally using Random Design Group (RAK) factorial pattern 2x3. The first factor is the compositions of mangrove (single & mix) and the second factor is inlet width (1 m. 2m, and 3 m). The result of the ANOVA analysis showed that height, diameter growth on each inlet width and composition of different mangrove showed that there is no significant difference of growth during two months research. However on L3K1 (3 meters inlet width with a single mangrove composition) produce heigh and diameter growth were better than on the other inlet width and mixture composition of mangrove.  Key words : Growth, Seedling, R. mucronata, Silvofishery.
BRYOFLORA TERESTRIAL DI ZONA TROPIK GUNUNG UNGARAN, JAWA TENGAH Heti Ergiana; Erry Wiryani; Jumari J
Jurnal Akademika Biologi Vol. 2 No. 1 Januari 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The Ungaran mount  is a various kind area of biodiversity, either flora or fauna. One of the flora biodiversity there is bryoflora. Bryoflora are classified  into two groups, terrestrial and epiphytic. The bryoflora researchs has been widely applied in the java island, but the related  publication of terrestrial bryoflora in the Ungaran mount Central Java are rarely found. The purpose of the research is to explain the diversity of  terrestrial bryoflora in the tropical zone (at altitude 750 m asl, 980 m asl, and 1100 m asl). The method for bryoflora sampling used quadrate with purposive sampling method. The result on this research  in tropical zone of Ungaran mount found 50 spesies of bryoflora that were identified in to three division : Bryophyta, Marchantiophyta, and Anthocerophyta. The percentage of the Bryophyta that was found are: Bryophyta 56 % (34 species), Marchantiophyta 40 % (15 species), and Anthocerophyta 4 % (1 species). Based on the height of station were identified 26 species at altitude 750 m asl, 17 species at altitude 980 m asl, and 24 species at altitude 1100 m asl. Keywords: Terrestrial Bryoflora, Tropical Zone, Ungaran Mount
PRODUKSI INULINASE DAN KECEPATAN PERTUMBUHAN SPESIFIK ISOLAT KHAMIR DUCC Y-015 PADA BERBAGAI KONSENTRASI TEPUNG UMBI DAHLIA Dani Ika Purwaningsih; Arina Tri Lunggani; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulin is an abundant compound in the nature after starch. Hydrolytic enzyme called inulinase is able to catalyse hydrolysis reaction of inulin polysaccharides into fructose and fructooligosaccharide (FOS). Yeast isolateDUCC Y-015 is one of the microbes that is able to produceinulinase. The action of inulinase enzyme associated with the growth of the isolate. Specific growth rate is one of the measurement of growth parameters; itis defined as the increase in cell biomass per unit time in the growth phase of the microbes. This study aims to examine theinulinase production and specific growth rate of yeast isolate DUCC Y-015 on the medium with various concentrations of dahlia tuber flour. The measurement of growth was done by weighing the cell dry weight and inulinase activity test was done by calculating the reduction sugar using DNS method. The used concentrations of dahlia tuber flourin the medium were 2%, 3%, 4% and 5%. Inulinase activity in each treatment was 0,111 IU/ml, 0,147 IU/ml, 0,113 IU/ml and 0,119 IU/ml, respectively, whereas the specific growth rate was 0,0062 hour-1, 0,0081 hour-1, 0,0077 hour-1, 0,0052 hour-1, respectively. The resultswere tested using the Mann-Whitney Test and theKruskal-Wallis Test. According to the results, the most optimuminulinase production and specific growth rate (µ) of yeast isolate DUCC Y-015 on dahlia tuber flour medium was treated with 3% concentration of dahlia tuber flour.Keywords: Dahlia Tuber Flour, Yeast Isolate DUCC Y-015, Specific Growth Rate, Inulinase Activity

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