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Biodiversitas Journal of Biological Diversity
Published by Universitas Sebelas Maret
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 14 Documents
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Search results for , issue "vol. 9 no. 1 (2008)" : 14 Documents clear
Synthesis of flavonoid-α-glicoside through transglycosylation by enzyme and its activities as antioxidant RINI HANDAYANI; JOKO SULISTYO
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090101

Abstract

Flavonoid-α- glycoside was synthesized using enzyme of CGT-ase (EC.2.4.1.19) which was isolated from cultivated of Aspergillus oryzae. CGT-ase enzyme has optimum capability at the temperature of 40°C, pH 7 yielded 1.87 unit/mL while at pH 6 was 1.11 unit/mL. The pretest of CGT-ase transfer activity was carried out using resorcinol as an acceptor and commercial starch solution as the glucosyl donor. Subsequently, acceptor was replaced by crude extract of ginger, and wheat starch as donor. The other product of hydrolysis was separated by column chromatography, monitored by TLC which was showed a single spot. The Rf value was compared with the Rf value of arbutin standard, the Rf values were about the same which were 0.85 for product and 0.87 for arbutin standard. The sugar total of product synthesis was determined by the Dubois method, which was 628.0 ppm. The value is equivalent to 0.032% of the original starch. The antioxidant activity was analyzed by β-carotene method discoloration. The result showed that the strong antioxidant activities were in the following order: BHT>product>arbutin>crude extract of ginger.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Aspergillus oryzae ,CGT-ase, flavonoid-α-glicosidea
Isolation and selection of local isolates of chitinolitic bacteria that potent to biocontrol of larva stadia of Aedes aegypti L. SRI PUJIYANTO; ENDANG KUSDIYANTINI; MOCHAMMAD HADI
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090102

Abstract

The dengue fever was the most dangerously epidemic in Indonesia, so it is important to prevent the population of Aedes aegypti L. The larva stages of Ae. aegypti had an exosceleton from chitin, so if its exosceleton had been degraded the larva would be died. Many bacteria had chitin degradation activities, and the bacteria had potential as a biocontrol for Ae. aegypti larva stages. This research was got many of the local chitinolitic bacteria that had been potential as a biocontrol for Ae. aegypti larva stages. The chitinolitic bacteria were isolated with selective agar medium. The sources of isolates were collected from Central Java and West Java water resources. Selected isolates was done to get the higher chitinolitic activity in mineral water medium. The isolate LMB1-5 was potential as a bioinsecticide for Ae. aegypti larva stages. This isolate decreased 86.7% of the larva population in 7 days.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: chitinolitic bacteria, dengue fever, Aedes aegypti L, biocontrol
The effect of maturated stages and soaking treatment of water and GA3 hormone on Brucea javanica (L.) Merr germination NINIK SETYOWATI; NING WIKAN UTAMI
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090104

Abstract

Research on the effect of maturated stages and soaking treatment of water and Giberrelic Acid hormone on Brucea javanica germination was carried out at Macropropagation Laboratory, Research Centre of Biology, LIPI, Cibinong-Bogor. The research study arranged by Factorial in Completely Randomized Design with 2 factors and 3 replications, each replication with 20 seeds sample. The first factor was maturated stage of fruits with 3 levels factor i.e. green, brown and black peels, and the second factor was soaking treatment with 6 levels factor i.e. 0 (control), 1, 3 days in water, 500, 1000, 1500 mg/L in GA3 solution. The results showed that the maturated stage had significant on seeds germination of B. javanica. The black peel of maturated stage of fruit was the best factor on chosen on B. javanica seedling, in comparing with brown and green peels. Soaking treatment in GA3 solution was effective on trigger of seeds germination of B. javanica. The combination treatment of black peel maturated stage with 1500 mg/L GA3 solution were the best result for all parameter observation i.e. on the first germination (4 days), rich germination 50% (10 days), germination percentage (93.33%) and germination value (7.074). But in the control were first germination (10 days), rich germination only 20% (no rich 50%), germination percentage (93.33%) and germination value (0.148).© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: maturity stages, soaking, water, GA3, seeds germination, Brucea javanica (L.) Merr
The growth of cassava (Manihot esculenta Crantz) on various alternative gelling agents DODY PRIADI; HANI FITRIANI; ENNY SUDARMONOWATI
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090103

Abstract

Gelling agents which is an important component in plant tissue culture media is considered expensive which causes high cost of plant micropropagation in developing countries. The objective of the study was to evaluate various commercial starches (hunkue, sago, tapioca, maize and arrowroot) and food agars for substitution of standard technical agar which commonly used in tissue culture medium. Young stem cuttings with five buds of cassava (Manihot esculenta Crantz. genotype Iding and Gebang) cultured on MS hormone-free media solidified with those starches and agars. Parameters observed were total and length of shoots and rate of contamination. Result of studyshowed that the highest total shoots (2.45) on genotype Iding obtained from Agar Swallow 0.8% (control), meanwhile on Gebang (2.85) obtained from tapioca 25%. The highest shoot length on genotype Iding (17.2 mm) obtained from maize, meanwhile on Gebang obtained from agar Sinar Kencana 2% (8.95 mm). Contamination rate of explants caused by bacteria or fungi on genotype Iding was 30-70%, meanwhile on Gebang was 20-60%. Further study needs to be done to evaluate more gelling agents from different sources and theircombinations.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: medium, gelling agents, agar, starch, cassava (Manihot esculenta Crantz).
Orchids inventory in Sintang Regency, West Kalimantan ESTI ENDAH ARIYANTI; PA’I PA’I
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090106

Abstract

Orchid is one of ornamental plants which have commercial value. Therefore most species are becoming threatened or even endangered because of over exploitation. In addition, its natural habitat is also decreasing. Conservation must be done urgently, both by in situ and ex situ conservation, which can be started by orchid inventory. The orchid inventory was done in TWA Bukit Kelam, TWA Baning and several places in Regency of Sintang, West Kalimantan. The result showed that there were 40 species belonged to 27 genera, which 32 species of them (20 genera) were epiphytic orchids and 8 species (7 genera) were terrestrial orchids.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: inventory, orchid, Sintang, West Kalimantan
Ecological study on mangrove forest in East Coast of North Sumatra ONRIZAL ONRIZAL; CECEP KUSMANA
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090107

Abstract

Ecological studies on mangrove forest in East Coast of North Sumatra have been carried out with field work in transect method and laboratory analyses. This study would be covered on floristic composition, abrasion, green belt, soil properties, and water quality of mangroves. Land system map and landsat TM imagery (year 1996 coverage) as main material in this study were used and overlay to determine training area. Based on vegetation inventory found that 20 mangrove species and by vegetation analyses, we known that Avicennia marina was as dominant tree species of seedling and sapling stage. Tree stage was not found in the area, yet. Environment properties of the mangrove area were suitable for mangrove growth and rehabilitation with the exception of pyrite content in the mangrove soil. Average of mangrove green belt was 25 m with range from 10 to 80 m in KJP (Kajapah) land system and 30 m with range 10 to 50 m in PTG (Putting) land system. Abrasion rate in the area was very high, i.e. 6 m per year in KJP land system, and 10 m per year in PTG land system.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: mangrove, abrasion, green belt, soil properties, water quality, North Sumatra
Alkaloid and Phenolic Compounds of Rafflesia hasseltii Suringar and its host Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. in Bukit Tigapuluh National Park, Riau: A Preliminary Study NERY SOFIYANTI; NINIK NIHAYATUL WAHIBAH; DEDEK PURWANTO; EDY SYAHPUTRA; KAMARUDIN MAT SALLEH
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090105

Abstract

Two alkaloid compounds (nicotine and caffeine) together with three phenolic compounds (catechin, proanthocyanidin and phenolic acid) were firstly detected in Rafflesia hasseltii and its host, Tetrastigma leucostaphylum in Bukit Tigapuluh National Park, Riau. The content of all compounds is higher in R. hasseltii than its host.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Rafflesia hasseltii, Tetrastigma leucostaphylum, alkaloids, phenolic compounds, HPLC.
Pollen viability and its effect on fruit set of oil palm (Elaeis guineensis Jacq.) ALFIN WIDIASTUTI; ENDAH RETNO PALUPI
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090109

Abstract

The research was aimed at studying (1) the decline of pollen viability during storage, and (2) the effect of pollen amount on fruit set of oil palm (Elaeis guineensis Jacq.). The experiment was conducted at PT. Dami Mas Sejahtera and PT. Sinar Mas Agro Resource and Technology (SMART) Tbk, Riau from February to August 2004. The first experiment was investigated up to six months storage period in the refrigerator, whereas in the second experiment a randomized complete block design with two factors was used: length of storage, i.e. 0, 1 and 2 months and amount of pollen, i.e. 0.022, 0.044, 0.066, 0.088, and 0.11 g mixed with powder to 10g to pollinate an inflorescence. The result showed that the viability of pollen started to decline three months after storage from about 92% to 83%, and declined to about 75% after six months of storage. Result of the second experiment showed that storage of pollen up to two months did not affect percentage ofnormal fruit, although the percentage of parthenocarpic fruits was decreased. This could be due to the high viability of pollen as the viability was remained high (about 90%) after being stored for two months in the refrigerator. Pollen with high viability could be used in a smaller amount to pollinate a female inflorescence without affecting fruit set of about 70-76%.SD037 had a higher reproductive success than SD038 and SD39.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: pollen storage, fruit set, amount of pollen, oil palm, Elaeis guineensis
The inventory of mollusc species and its potent on seagrass bed in Kei Kecil Islands, Southeast Moluccas AGUS KUSNADI; TEDDY TRIANDIZA; UDHI EKO HERNAWAN
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090108

Abstract

Inventory of mollusk species and its potential on sea grass bed in Kei Kecil islands, Southeast Moluccas have been conducted by using quadrate-transect line method. The study was carried out in February-August 2007. There were 103 species of mollusk observed, 80 species belong to gastropods and 23 belong to bivalves. From the total species, 72 of them were reported as the potential species and the others still unknown. Most of potential group was used as food resources (55 species) and other species were potential as decoration, souvenir, ornament, and accessories (31 species). Some species were potential for building material, knife tool, barter tool and bioactive compounds. The top shells (Trochus niloticus), the giant clams (Tridacna spp. and Hippopus hippopus) and the abalone (Haliotis spp.) were the major trading commodities for shellfish fisheries.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: inventory, mollusk, potency, Kei Kecil.
The use of miswak (Salvadora persica L.) extract to increase immune response of common carp (Cyprinus carpio L.) in cage HENNI SYAWAL; SYAFRIADIMAN SYAFRIADIMAN; SYAUQI HIDAYAH
Biodiversitas Journal of Biological Diversity Vol. 9 No. 1 (2008)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d090110

Abstract

The aim of this research was to increase immune response of common carp (Cyprinus carpio L.) in cage to microbial infection and environmental change. This research used completed randomized (RAL) one factor, four treatments and each of three replicates. This treatment was immersion extract miswak (Salvadora persica L.) dose 0, 4.5, 9, and 13.5 g/L. The cage 1 x 1 x 1.2 m, with density 50 fish/ cage (average initially 10-15 cm). Immersion was done once time a week along three weeks, l0 min, and after it fish reared in cage along 30 days. The response parameters assayed were survival rate, haematocrit, erythrocytes, leukocytes, and water quality. The results of experiment showed that the dose optimum 9 mg/L (P2) with survival rate 99.66% and P0 (70.66%). The level of haemotocrit was 18.93-34.36%, erythrocytes 3.597.000-6.112.000 cells/mL3, leukocytes 24.748-143.070 cells/mL3. It was concluded, which miswak extract at a dosage 9 mg/L can increase survival rate and immune response of common carp.© 2008 Jurusan Biologi FMIPA UNS SurakartaKey words: Salvadora persica L., Cyprinus carpio L., haematology.

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