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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 7 Documents
 1 
Search results for , issue "vol. 1 no. 1 (2009)" : 7 Documents clear
Effect of VCO to leucocyte differential count, glucose levels and blood creatinine of hyperglycemic and ovalbumin sensitized Mus musculus Balb/c NOOR SOESANTI HANDAJANI; RUBEN DHARMAWAN
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010101

Abstract

Abstract. Handajani NS, Dharmawan R. 2009. Effect of VCO to leucocyte differential count, glucose levels and blood creatinine of hyperglycemic and ovalbumin sensitized Mus musculus Balb/c. Nusantara Bioscience 1: 1-8. Chemical medicines and insulin can decrease glucose blood levels in hyperglycemic patients with macrovascular side effects. The quality and quantity of leucocytes influence diabetes and allergy incidences. Lauric acid within VCO reports decreased glucose blood level of diabetes and some allergy incidents. The purpose of the study is to know the effect of VCO on the glucose blood level, differential leucocyte count, and creatinine blood level on hyperglycemic and normoglycemic ovalbumin-sensitized mice. Forty-five (45) males (mice) of Mus musculus Balb/c with an average weight of 35 g are divided into nine groups with five repetitions; those are four non-alloxan groups and five alloxan-induced hyperglycemic groups. From the 22nd day to the 36th day, they are sensitized to ovalbumin as an allergen. A blood sample was obtained by orbital vena using heparin as an anticoagulant to measure glucose blood level by GOD method to 6 times, on 1st, 4th, 18th, 22nd, 32nd, and 37th days, then are tested by ANOVA followed by DMRT 0.05. On the 37th day, differential leucocytes are determined, the blood level is counted, and then compared to the normal value. This study showed that within differential leucocytes count of hyperglycemic mice, neutrophile percentage was much lower than the normal value (3.22%), and lymphocyte percentage was much higher than the normal value (94.54%). Consumed 0.003 mL/35 g VCO more 18 days decreased glucose blood level on hyperglycemic mice, decreased basophile percentage of ovalbumin-sensitized mice, normalized neutrophile percentage no increased creatinine blood level.
Body weight and statistic vital of Texel sheep in Wonosobo District by giving the ramie hay as an additional woof AGUS KUNTJORO; SUTARNO SUTARNO; OKID PARAMA ASTIRIN
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010102

Abstract

Abstract. Kuntjoro A, Sutarno, Astirin OP. 2009. Body weight and statistics vital of Texel sheep in Wonosobo District by giving the ramie hay as an additional woof. Nusantara Bioscience 1: 23-30. This research is aimed to observe the body weight and vital statistic measurement of 50 Texel sheep. Sheep are classified into five treatments of giving woof P0 (giving greenish tree woof without concentrate), P1 (giving greenish woof and concentrate without adding the ramie hay/0%) concentrate), P2 (giving greenish woof and concentrate by adding 10%) ramie hay), P3 (giving greenish woof and concentrate by adding 20%) ramie hay), P4 (giving greenish woof and concentrate by adding 30%) ramie hay), every treatment was repeated 10 times. The result shows that even though it can’t replace the concentrate function, adding ramie hay as much as 10%), 20%) and 30%) on sheep woof can increase the body weight’s growth, respectively 186.67 g/day, 153.34 g/day dan 103.34 g/day. The addition of ramie hay 10%), 20%) and 30%) can increase the addition of statistic vital’s measurement on breast of sheep livestock 1.20 cm); 0.95 cm) and 0.90 cm); the addition of statistic vital measurement on the body length of sheep livestock 0.05 cm); 1.00 cm) and 0.75 cm) and also the addition of breast width is 1.50 cm); 0.15 cm) and 0.3 cm). Meanwhile, the addition of ramie hay on livestock woofs can only increase the addition of vital statistical measurement on the breast at giving 30%) as big as 0.15 cm). It is needed to know further about giving ramie hay by comparing hay of different concentrations of leaf and stems.
Characterization of white grubs (Melolonthidae: Coleoptera) at salak pondoh agroecosystem in Mount Merapi based on isozymic banding patterns SRI WARDANI; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010103

Abstract

Abstract. Wardani S, Sugiyarto. 2009. Characterization of white grubs (Melolonthidae: Coleoptera) at salak pondoh agroecosystem in Mount Merapi based on isozymic banding patterns. Nusantara Bioscience 1: 38-42. The aim of this research was to know the characteristics of white grubs (Melolonthidae: Coleoptera) based on isozyme banding patterns. This research was conducted at Sleman, Yogyakarta, and Magelang-Central Java for morphological purposes. The sample was taken from 5 places with different heights in which five samples were taken from each location. The method used in this research was polyacrylamide gel electrophoresis (PAGE) using the vertical type. The enzyme system used in this research were peroxidase and esterase to detect the isozyme banding patterns. The results showed a variation in isozyme banding patterns of white grubs (Melolonthidae: Coleoptera) at salak pondoh agroecosystem in Mount Merapi’s slope (peroxidase in station II and IV while esterase in station III and V). It means that genetic variation on the white grubs population at salak pondoh agroecosystem in Mount Merapi’s slope was found. The environmental condition also influenced the isozyme banding pattern’s appearance because each location had a different state.
Review: Effect of global warming on plant evolution and diversity; lessons from the past and its potential recurrence in the future AHMAD DWI SETYAWAN
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010104

Abstract

Abstract. Setyawan AD. 2009. Effect of global warming on plant evolution and diversity; lessons from the past and its potential recurrence in the future. Nusantara Bioscience 1: 43-52. Lessons from the past show that global warming and glaciation are repeated natural cycles; the trigger factor is not always the same. Still, global warming is always accompanied by elevated levels of CO2 and greenhouse gases in the atmosphere, which causes the other rising global temperatures. Present and destruction of various plants and other living things continue to happen from time to time. Every era has its own life form, as a mirror of global environmental conditions at the time. Biodiversity is not always the same between one period of global warming and the subsequent global warming or one period of glaciation with the next glaciation. However, new breeds always show traces of the evolution of their ancestors. Man is one of the agents of global warming that began with developing agricultural systems since 8,000 years ago. The impact of climate change due to global warming should continue to be wary of. Based on past experience, global warming is always followed by mass extinctions, but various life forms will still survive even though their shape is almost certainly not the same as before. Living organisms that can survive will evolve into new taxa different from their parental taxa. Humans who were present at that time probably were not men present at this time, given Homo sapiens may have been extinct for not being able to adapt or otherwise has evolved into a new man who may no longer show characteristics of human wisdom.
Biomass, chlorophyll and nitrogen content of leaves of two chili pepper varieties (Capsicum annum) in different fertilization treatments SUHARJA SUHARJA; SUTARNO SUTARNO
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010102

Abstract

Abstract. Suharja, Sutarno. 2009. Biomass, chlorophyll and nitrogen content of leaves of two chili pepper varieties (Capsicum annum) in different fertilization treatments. Nusantara Bioscience 1: 9-16. This study aims to determine the influence of various fertilization treatments on biomass, chlorophyll, and nitrogen content of leaves from two varieties of chili, Sakti (large chili) and Fantastic (curly chili). The study was conducted in the village of Gatak, Karangnongko sub-district, Klaten District, Central Java, from September 2006 to March 2007. The study used a complete block design with two factorials, chili varieties, and fertilizer treatment. Fertilization treatments includes no fertilizer (control) (P1); manure 2 kg/plant (P2), manure (1 kg/plant) + chemical fertilizer (ZA, SP-36, KCl = 2: 1: 1) + NPK (P3); and manure (1 kg/plant) + chemical fertilizer (SP-36: KCl = 1:1) + liquid organic fertilizer (P4). According to Harborne (1987), chlorophyll content was measured, whereas leaf nitrogen concentration was measured with the Kjeldahl method. Data were analyzed using ANOVA followed by DMRT. The results showed that the Fantastic chili fertilizer treatment affected the biomass and chlorophyll-a but did not affect chlorophyll b, total chlorophyll, and leaf nitrogen. On the curly chili, fertilizer treatment affected fresh plant weight, chlorophyll a, and total chlorophyll but did not affect dry weight, fresh fruit weight, chlorophyll b, and leaf nitrogen. It is, therefore, recommended to use the formulation of manure + chemical fertilizer (SP-36: KCl = 1: 1) + liquid organic fertilizer in the cultivation of chili.
Effect of IAA and GA3 toward the growing and saponin content of purwaceng (Pimpinella alpina) DASIYEM FATHONAH; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010103

Abstract

Abstract. Fathonah D, Sugiyarto. 2009. Effect of IAA and GA3 toward the growing and saponin content of purwaceng (Pimpinella alpina). Nusantara Bioscience 1: 17-22. The aims of this research are to examine (i) the effect of IAA and GA3 in different concentrations on the growth of the plants and (ii) the saponin contained inside the P. alpina leaves. The research was done in Sikunang Village, Kejajar Subdistrict, Wonosobo District, Central Java, from July to November 2007. The Completely Random Design used the experimental methods with two factors to analyze this experiment. The first treatment gives IAA and GA3; the second provides different IAA and GA3 concentrations. These experiments were repeated three times. Variables measured in this research were plant growth consisting of the number of leaves, their height, width, wet weight, and dry weight. The chemical compound of the secondary metabolite in the form of leave saponin was employed. Analysis of Variance (ANOVA) analyzed the result, then continued to Duncan Multiple Range Test in 5% level to analyze the real difference between those treatments. The result showed that giving IAA and GA3 differently affects the growth of P. alpina. In variable of the height, the optimal wet weight and dry weight in GA3 treatment was 50 ppm; an optimum number of leaves in GA3 treatment was 50 ppm, whereas the leaves width in IAA treatment was 200 ppm and GA3 treatment was 75 ppm. Optimum saponin treatment was IAA 200 ppm and GA3 25 ppm.
Protein expression on Cr resistant microorganism using electrophoresis method UMI FATMAWATI; SURANTO SURANTO; SAJIDAN SAJIDAN
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010105

Abstract

Abstract. Fatmawati U, Suranto, Sajidan. 2009. Protein expression on Cr resistant microorganism using electrophoresis method. Nusantara Bioscience 1: 31-37. Hexavalent chromium (Cr(VI)) is known as toxic heavy metals, so the need is reduced to Cr(III) is much less toxicity. Pseudomonas aeruginosa, Pseudomonas putida, Klebsiella pneumoniae, Pantoea sp., and Saccharomyces cerevisiae are resistant Cr(VI) microorganisms and can reduce Cr(VI). The aim of this research is to know ability of the microorganism to reduce Cr(VI) and to know the protein band pattern between Cr(VI) resistant microorganisms and non-resistant microorganisms inoculated on LB broth. SDS-PAGE was used to identify protein expression. At the same time, Cr(VI) concentration was identified by the 1.5 diphenyl carbazide method. The quantitative data were analyzed by two factorial ANOVA that continued with DMRT at a 1% level test. The qualitative data, i.e., protein expression analyzed by relative mobility (Rf). The results showed that the ability of microorganisms to reduce Cr(VI) at an initial concentration of 0.5 ppm, one ppm, five ppm, and 10 ppm might vary; the average percentage of the ability of each microorganism in reducing Cr(VI) is P. putida (65%) > S. cerevisiae (64.45%) >. P. aeruginosa (60.73%) > Pantoea sp. (50.22%) > K. pneumoniae (47.82%) > without microorganisms (34.25%).ListenRead phonetically The adding microorganisms have significantly influenced toward reduction of Cr(VI). The SDS-PAGE shows that protein expression between resistant and not resistant microorganisms are no different, but resistant microorganisms have more protein (protein band is thicker).

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