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Nusantara Bioscience
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 7 Documents
Search results for , issue "vol. 1 no. 2 (2009)" : 7 Documents clear
Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment MARTI HARINI; OKID PARAMA ASTIRIN
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010201

Abstract

Abstract. Harini M, Astirin OP. 2009. Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment. Nusantara Bioscience 1: 53-58. This study aims to determine the treatment effect of VCO on blood cholesterol levels in hypercholesterolemic white rats (Rattus norvegicus L.). This study used 25 male rats of Wistar strain divided into five treatment groups, namely: control, simvastatin (1.3 mL/270 g BW), cholesterol (9:1 lard), VCO 1 (1 mL/270 g BW), and VCO 2 (1.3 mL/270 g BW). Treatment was given orally. Total cholesterol, LDL, and HDL cholesterol levels were measured on days 1, day 14, and 28. Cholesterol data (total cholesterol, LDL, and HDL) were analyzed by ANOVA and followed by a contrast test at a significance level of 5%. The results showed that treatment of VCO at different doses significantly affected the decrease in blood total cholesterol blood LDL levels, increasing blood HDL in the hypercholesterolemic white rat.
Effect of manure and NPK to increase soil bacterial population of Azotobacter and Azospirillus in chili (Capsicum annum) cultivation MUJIYATI MUJIYATI; SUPRIYADI SUPRIYADI
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010202

Abstract

Mujiyati, Supriyadi. 2009. Effect of manure and NPK to increase soil bacterial population of Azotobacter and Azospirillum in chili (Capsicum annum) cultivation. Nusantara Bioscience 1: 59-64. The objectives of this research were to find out the increase number of two bacterials populations, Azotobacter and Azospirillum, due to the use of manure fertilizer. The exsperiment was conducted using group randomly designed with two treatments. The plant populations were treated (i) whithout fertilizer as the control, (ii) with manure fertilizer, and (iii) with NPK fertilizer. Data was experimentally collected by planting chili in several plots treated by manure, with three replications. The field experiment was conducted in Gathak Village, Karangnongko Sub-district, Klaten District, Central Java. The data collected consist of the total population of Azotobacter and Azospirillum, nitrogen content in soil and the chili yield. The primary data of research were analyzed using ANOVA test and followed by LSD test, with the degree of significance by 95% .The results showed that the manure fertilizer can increase the population of bacteria as many as 0.02% (Azotobacter) and 0.46% (Azospirillum) when they were compared to the control one. So that it can increase the soil fertility when they were used in long time. Therefore increasing the nutrient availability in the soil was occurred. Application of manure fertilizer could increase the total nitrogen content in the soil and it is very useful for the fertilizing of plants.
Characterization of white grub (Melolonthidae; Coleoptera) in salak plantation based on morphology and protein banding pattern KRISNANDARI TITIK MARYATI; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010204

Abstract

Abstract. Maryati KT, Sugiyarto. 2010. Characterization of white grub (Melolonthidae; Coleoptera) in salak plantation based on morphology and protein banding pattern. Nusantara Bioscience 1: 72-77. This research aims to find out the white grub (Melolonthidae; Coleoptera) variability based on the morphological characteristic and protein banding pattern found in "salak pondoh" farm in Regencies of Sleman, Yogyakarta and Magelang, Central Java. Each area has five sampling points. Morphological analysis on white grub was conducted using descriptive method and analysis on protein banding pattern was conducted using qualitative analysis based on the presence or absence of band pattern on the gel, and qualitatively based on the relative mobility value (Rf) of protein. The result indicated that the white grub in Sleman and Magelang, based on morphology characteristic is only one species, namely Holothricia sp. Based on the protein banding pattern, the white grub sample has differences in protein band number and molecular protein weight.
Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties DWI HASTUTI; SURANTO SURANTO; PRABANG SETYONO
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010205

Abstract

Hastuti D, Suranto, Setyono P. 2009. Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties. Nusantara Bioscience 1: 78-83. The aim of this research to find out the Adenium obesum variation from six varieties, namely: obesum, cery, red lucas, red fanta , white bigben and harry potter based on morphology, karyotype, as well as protein banding pattern. The chromosome preparation was made using semi-permanent squash method from the tip of root plant; while protein banding pattern was made using SDS-PAGE method. Qualitative data included shape and color of the leave and flower described from each variety. Data were presented in morphometry and analyzed using ANOVA and then followed by DMRT with 5% of confidence levels, indicated significance difference. Protein banding pattern, the root, stem, leave and all organs were analyzed using Hierarchical Cluster Analysis method with Average Linkage (between Groups) using SPSS 10.0. The result of research shows that the six A. obesum varieties have morphological character with no variation of light green to dark green leave, not hairy, smooth leave bone, meanwhile for light red to dark red flower crown color although some of them are white and the same funnel color, yellow. All varieties of A. obesum have same number of chromosome, 2n = 22 and shows the difference ranging from 2.56 to 5.13 μm. In the banding pattern formed qualitatively, there is variation among the six varieties.
Variability analysis of Sukun durian plant (Durio zibethinus) based on RAPD marker ISMI PUJI RUWAIDA; SUPRIYADI SUPRIYADI; PARJANTO PARJANTO
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010206

Abstract

Abstract. Ruwaida IP, Supriyadi, Parjanto. 2009. Variability analysis of Sukun durian plant (Durio zibethinus) based on RAPD marker. Nusantara Bioscience 1: 84-91. The purpose of the study is to assess the diversity of the durian varieties of Sukun, Sunan, Kani, Monthong, and Petruk; and Sukun durian variety is grown in different regions based on RAPD markers. Materials research is durian leaves of Sukun, Sunan, Kani, Monthong and Petruk from Ranukutri Garden Seeds, Karanganyar, and also Sukun durian leaf from Gempolan Karanganyar, Jepara and Salatiga. Then performed DNA analysis on samples of leaves that began the isolation of DNA, test of DNA quantity and quality, primer selection, and amplification with PCR. Visualization of PCR performed by electrophoresis using EtBr that generate DNA bands. DNA bands were then analyzed with NTSYS program to get durian diversity among varieties of durian and between Sukun durian at different planting areas. Six RAPD polymorphic primer was used, namely OPA-01, OPA-02, OPA-07, OPA-16, OPA-18, and OPA-19. Banding pattern of DNA amplification show the existence of diversity among varieties of Sukun durian, Sunan, Kani, Monthong and Petruk and show the diversity of Sukun durian grown in different areas. Dendrogram indicated that five durian varieties tend to segregate, whereas Sukun, Sunan, Monthong, and Petruk in one group, which split with Kani. Dendrogram also indicated that Sukun planted in different regions resulted in two groups, Sukun from Gempolan Karanganyar and Salatiga in one group, while the second group consisted of Sukun from Ranukutri seed garden Karanganyar and Jepara. generate DNA bands. DNA bands were then analyzed with NTSYS program to get durian diversity among varieties of durian and between Sukun durian at different planting areas. Six RAPD polymorphic primer was used, namely OPA-01, OPA-02, OPA-07, OPA16, OPA-18, and OPA-19. Banding pattern of DNA amplification show the existence of diversity among varieties of Sukun durian, Sunan, Kani, Monthong and Petruk and show the diversity of Sukun durian grown in different areas. Dendrogram indicated that five durian varieties tend to segregate, whereas Sukun, Sunan, Monthong and Petruk in one group, which split with Kani. Dendrogram also indicated that Sukun planted in different regions resulted in two groups, Sukun from Gempolan Karanganyar and Salatiga in one group, while the second group consisted of Sukun from Ranukutri seed garden Karanganyar and Jepara.
Diversity of Sonneratia alba in coastal area of Central Java based on isozymic patterns of esterase and peroxidase AHMAD DWI SETYAWAN
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010207

Abstract

Abstract. Setyawan AD. 2009. Diversity of Sonneratia alba in coastal area of Central Java based on isozymic patterns of esterase and peroxidase. Nusantara Bioscience 1: 92-103. The study was intended to observe the diversity and the relationship of Sonneratia alba in southern and northern coast of Central Java Province based on isozymic patterns of esterase and peroxidase. This research was conducted in July until December 2003, at six sites, i.e. Wulan (WUL), Juwana (JUW), Pasar Bangi (PAS), in the northern coast, and Bogowonto (BOG), Motean (MOT), and Muara Dua (MUA) in the southern coast. The laboratory assay was conducted in Central Laboratory of Mathematics and Natural Sciences, Sebelas Maret University (UNS) Surakarta. The seedling plant samples that were collected from six mangrove habitats transplanted in greenhouse in the laboratory. There were 20 individual samples each site. The result indicated that the individual of S. alba of the same or near location has the same genetic diversity in common, because the genetic exchange on the same population was higher than on a different population. Therefore, the populations of S. alba from northern coast had higher similarity each other than southern coast one, on the other way the populations from southern coast had higher similarity each other than northern coast one.
Nitrogen content, nitrate reductase activity, and biomass of kimpul (Xanthosoma sagittifolium) on shade and nitrogen fertilizer variation ISNAINI CHOIRUL LATIFA; ENDANG ANGGARWULAN
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010203

Abstract

Abstract. Latifa IC, Anggarwulan E. 2009. Nitrogen content, nitrate reductase activity, and biomass of kimpul (Xanthosoma sagittifolium) on shade and nitrogen fertilizer variation. Nusantara Bioscience 1: 65-71. The aims of this research were to study the effect of shade and nitrogen fertilizer variation and also their interaction to nitrogen content, nitrate reductase activity, and biomass of kimpul (Xanthosoma sagittifolium (L.) Schott.). This research was arranged in randomized complete block design (RCBD) with two factor, they were shade variation which contained 3 levels (0%, 50%, and 75% of shade) and nitrogen fertilizer dosage variation which contained 4 levels (0; 0.0625; 0.125; dan 0.25 g ZA/kg of soil of the dosage of ZA fertilizer). The variables of the research were nitrogen content, nitrate reductase activity, fresh weight, dry weight, and shoot/root ratio. The collected data were analyzed with General Linear Model Univariate, and continued with Duncan's Multiple Range Test (DMRT) on 5% significantly levels. The result showed that the shade treatment affected nitrogen content, fresh weight, and shoot/root ratio. The fertilizer application and the interaction between shade and fertilizer application were affected to all variables. The treatment of 75% shade and 0.25 g ZA/kg of soil of ZA fertilizer dosage increased nitrogen content (5.32%) and fresh weight (420.88 g). The treatment without shade and 0.25 g ZA/kg of soil, dosage of ZA fertilizer increased nitrate reductase activity (260.58 µ mol NO2-/g/hour) and dry weight (53.92 g). The treatment of 50% shade and 0 g ZA/kg of soil of ZA fertilizer dosage increased shoot/root ratio (0.98).

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