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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 8 Documents
 1 
Search results for , issue "vol. 4 no. 3 (2012)" : 8 Documents clear
Determination of ethanol in acetic acid-containing samples by a biosensor based on immobilized Gluconobacter cells ANATOLY N. RESHETILOV; ANNA E. KITOVA; ALENA V. ARKHIPOVA; VALENTINA A. KRATASYUK; MAHENDRA K. RAI
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040301

Abstract

Abstract. Reshetilov AN, Kitova AE, Arkhipova AV, Kratasyuk VA, Rai MK. 2012. Determination of ethanol in acetic acid containing samples by a biosensor based on immobilized Gluconobacter cells. Nusantara Bioscience 4: 97-100. A biosensor based on Gluconobacter oxydans VKM B-1280 bacteria was used for detection of ethanol in the presence of acetic acid. It was assumed that this assay could be useful for controlling acetic acid production from ethanol and determining the final stage of the fermentation process. Measurements were made using a Clark electrode-based amperometric biosensor. The effect of pH of the medium on the sensor signal and the analytical parameters of the sensor (detection range, sensitivity) were investigated. The residual content of ethanol in acetic acid samples was analyzed. The results of the study are important for monitoring the acetic acid production process, as they represent a method of tracking its stages.
In vitro rapid multiplication of Stevia rebaudiana: an important natural sweetener herb SHIVAJI DESHMUKH; RAVINDRA ADE
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040303

Abstract

Abstract. Deshmukh S, Ade R. 2012. In vitro rapid multiplication of Stevia rebaudiana: an important natural sweetener herb. Nusantara Bioscience 4: 105-108. Stevia rebaudiana Bertoni, belonging to family Asteraceae and natural sweet plant, but due to poor seed viability, fertility, and vigor, Stevia cultivation is a challenging task. In the present study in vitro, rapid multiplication method was established for S. rebaudiana by inoculating explants on M.S. medium, supplemented with different combination of phytoharmone. The maximum number of shoots (18.3±0.8) was obtained on M.S. medium supplemented with BAP + KIN (1.5 + 0.5 mg/L). The highest rooting percentage (95.25) was observed with (IAA 0.1 mg/L). The rooted plants were successfully established firstly in soil with coco peat (1:1) and then directly in ordinary soil.
Eco-friendly synthesis and potent antifungal activity of 2-substituted coumaran-3-ones PRABHA SOLANKI; PRACHI SHEKHAWAT
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040302

Abstract

Abstract. Solanki P, Shekhawat P. 2012. Eco-friendly synthesis and potent antifungal activity of 2-substituted coumaran-3-ones. Nusantara Bioscience 4: 101-104. 3-halochromones (IIa-c and IIIa-c) have been synthesized by treating 1- (2-hydroxyphenyl)-3-methyl-1,3-propanediones (Ia-c) with bromine or sulphuryl chloride in dioxane respectively. These chromones were employed in the synthesis of 2-acetyl-coumaran-3-ones (IVa-f). These were subjected to Knoevenagel condensation to give 2-cinnamoyl coumaran-3-ones. In vitro assay and field trials of these compounds against Fusarium oxysporum were carried out to study the antifungal effect of target compounds. Compound Va was the most effective growth inhibitor of the pathogen, whereas Vc showed a little tendency and Vb, Vd, Ve and Vf hardly inhibit the growth.
Tagetes erecta mediated phytosynthesis of silver nanoparticles: an eco-friendly approach UMESH P. DHULDHAJ; SHIVAJI D. DESHMUKH; ANIKET K GADE; MADHU YASHPAL; MAHENDRA K. RAI
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040304

Abstract

Abstract. Dhuldhaj UP, Deshmukh SD, Gade AK, Yashpal M, Rai MK. 2012. Tagetes erecta mediated phytosynthesis of silver nanoparticles: an eco-friendly approach. Nusantara Bioscience 4: 109-112. Nanotechnology is a multidisciplinary field having applications in various fields like medicine, pharmacy, engineering, and biotechnology. An important step in nanotechnology is to develop simple and eco-friendly method for the nanomaterial synthesis. Here we describe simple and eco-friendly method for synthesis of silver nanoparticles by extract of Tagetes erecta plant leaves. The phytosynthesis (synthesis by plant) of silver nanoparticles was detected by color change from light-green to dark-brown. Synthesis of silver nanoparticles was confirmed by UV-Vis spectrophotometry, further characterization includes nanoparticle tracking analysis system (NTA) (LM20) and transmission electron microscopy (TEM). TEM analysis confirms the synthesis of the polydispersed spherical silver nanoparticles of 20-50 nm, with an average size of 30 nm.
Seed viability of Jatropha curcas in different fruit maturity stages after storage BAMBANG BUDI SANTOSO; ARIS BUDIANTO; I.D.P. MULIARTA ARYANA
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040305

Abstract

Santoso BB, Budianto A, Aryana IGPM. 2012.Seedviability ofJatropha curcasindifferentfruitmaturitystagesafterstorage.Nusantara Bioscience 4:113-117.Theeffect of fruit maturity stages and seed storage period to seed viability was investigated. Seedsamples ofWest Lombok, West Nusa Tenggara genotype ofJatropha curcas were collected fromastandof two-year-old trees at anexperimental field. The seed samples obtained were in four different stages of fruit maturity involving early maturity (green fruit),physiological maturity (yellow fruit), over maturity (brownies fruit), and senescence (black-dry fruit). The results showed that fruitmaturityand storage periodhad an influence on the seed viability ofJ. curcas. Thebest fruit maturity stage for seed viability includingseed oil content was found inyellow fruit and brownies fruit.For germination to be preserved, seeds could be stored in the ambientroom storage for at least five months. For the purpose of oil extraction, seed should preferably be stored not more than four monthsunder ambient room conditions.
Physiological response of Moringa oleifera to stigmasterol and chelated zinc ABDALLA EL-MOURSI; IMAN MAHMOUD TALAAT; MOHAMED ABDEL-GHANY BEKHETA; KARIMA GAMAL EL-DIN
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040306

Abstract

Abstract. El-Moursi A, Talaat IM, Bekheta MA, Gamal El-Din K. 2012. Physiological response of Moringa oleifera to stigmasterol and chelated zinc. Nusantara Bioscience 4: 118-123. Two pot experiments were carried out in the screen of the National Research Centre, Dokki, Giza, Egypt, during two successive seasons (2009/2010 and 2010/2011), respectively to study the effect of foliar spray with chelated zinc (100, 200 and 300 mg/L) and stigmasterol (50, 100 and 150 mg/L) on growth and chemical constituents of moringa plants (Moringa oleifera). The results indicated that treatment of plants with 300 mg/L chelated zinc or 150 mg/L stigmasterol significantly influenced the vegetative growth of moringa plants. The same treatments also significantly increased total sugars%, total protein%, total phosphorous and microelements contents in the leaves. The changes in the pattern of protein electrophoresis (SDS-PAGE) extracted from the newly formed leaves of moringa plants treated with different concentrations of chelated Zinc (Zn) or stigmasterol showed beneficial influences for improving plant growth, leaves quality and quantity.
Review: Biological fertilization and its effect on medicinal and aromatic plants KHALID ALI KHALID
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040307

Abstract

Abstract. Khalid KA. 2012. Review: Biological fertilization and its effect on medicinal and aromatic plants. Nusantara Bioscience 4: 124-133. The need to increase food production in most developing countries becomes the ultimate goal to meet the dramatic expansion of their population. However, this is also associated many cases with a reduction of the areas of arable land which leaves no opinion for farmers but to increase the yield per unit area through the use of improved the crop varieties, irrigation, and fertilization. The major problem facing the farmer is that he cannot afford the cost of these goods, particularly that of chemical fertilizers. Moreover, in countries where fertilizer production relies on imported raw materials, the costs are even higher for farmer and for the country. Besides this, chemical fertilizers production and utilization are considered as air, soil, and water polluting operations. The utilization of bio-fertilizers is considered today by many scientists as a promising alternative, particularly for developing countries. Bio-fertilization is generally based on altering the rhizosphere flora, by seed or soil inoculation with certain organisms, capable of inducing beneficial effects on a compatible host. Bio-fertilizers mainly comprise nitrogen fixes (Rhizobium, Azotobacter, Azospirillum, Azolla or blue-green algae), phosphate dissolvers or vesicular-arbuscular mycorrhizas and silicate bacteria. These organisms may affect their host plant by one or more mechanisms such as nitrogen fixation, production of growth promoting substances or organic acids, enhancing nutrient uptake or protection against plant pathogens. Growth characters, yield, essential oil and its constituents, fixed oil, carbohydrates, soluble sugars and nutrients contents of medicinal and aromatic plants were significantly affected by adding the biological fertilizers compared with recommended chemical fertilizers.
Short Communication: Effects of temperature on growth, pigment composition and protein content of an Antarctic Cyanobacterium Nostoc commune RANJANA TRIPATHI; UMESH P. DHULDHAJ; SURENDRA SINGH
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040308

Abstract

Abstract. Tripathi R, Dhuldhaj UP, Singh S. 2012. Short Communication: Effects of temperature on growth, pigment composition and protein content of an Antarctic Cyanobacterium Nostoc commune. Nusantara Bioscience 4: 134-137. Effect of temperature variation on biomass accumulation, pigment composition, and protein content was studied for the cyanobacterium Nostoc commune, isolated from Antarctica. Results confirmed the psychrotrophic behavior (optimum growth temperature 250C) of the cyanobacterium. Low temperature increased the duration of lag phase and exponential growth phase. Maximum increase in biomass was recorded on 24th day at 250C and on 12th day at 50C. The downshift from 25 to 50C had almost negligible effect on chl a content. Maximal protein content was recorded for cultures growing at 50C on 12th day. The carotenoids/chl a ratio was maximum (2.48) at 50C on 9th day. It remained almost constant for cultures growing at 5 and 350C. There was an induction in protein synthesis following downshift in temperature from 25 to 5?C.

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