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INDONESIA
LenteraBio
Published by Universitas Negeri Surabaya
ISSN : 22523979     EISSN : 26857871     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 10 Documents
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Search results for , issue "Vol 2, No 2 (2013)" : 10 Documents clear
KEKAYAAN JENIS KELELAWAR (CHIROPTERA) DI KAWASAN GUA LAWA KARST DANDER KABUPATEN BOJONEGORO Nurfitrianto, Hendrik; Budijastuti, Widowati; Faizah, Ulfi
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Bats are one of the animals that have the 2nd greatest diversity of species in the class of mammals after Rodentia. One of bats habitat is karst cave. East Java has more karst area like Tuban, Gresik and Bojonegoro. The bats of Lawa Cave at karst Dander have not studied yet. This study aimed to identify the species of bat in area Lawa Cave karst region Dander Bojonegoro. This research conducted during the months of February-March 2013. Bats collected directly from 4 stations in Lawa cave area using traps mistnet with size 3x10 m. Identification was done in the Taxonomy Laboratory Department of Biology UNESA. The results of this study obtained 5 species of bat: Rousettus amplexicaudatus, Hipposideros larvatus, Pipistrellus javanicus, Rhinolophus pusillus, Megaderma spasma.
AKTIVITAS ANTIFUNGI EKSTRAK DAUN KEDONDONG (SPONDIAS PINNATA) DALAM MENGHAMBAT PERTUMBUHAN ASPERGILLUS FLAVUS Fitriani, Silvia
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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People believe that stems, fruits, and leaves of kedondong plants (Spondias pinnata) are useful as a traditional medicine, such as the leaves and the fruit can be used as a cough medicine and the bark part can be used as a diarrhoea medicine. This research aimed to describe the antifungal activities kedondong leaves extract to inhibition on the growth of Aspergillus flavus and determine the effective concentration of kedondong leaves extract on inhibiting the A. flavus growth. The research were designed by randomized completely design with one factor treatment, namely concentration of the kedondong leaves extract with 4 replications. Antifungal activity test to be used solid dilution method. Kedondong leaves extract solution to be used with final concentration of 0%, 4%, 6%, 8%, 10% w /v. Observation was conducted after incubation for 7 days at room temperature. The parameters observed was the inhibition A. flavus growth with indicated size of the diameter mycelial colony. The results showed that the kedondong leaves extract had an inhibitory effect on mycelial colony A. flavus growth. Concentration 8% and 10% were effective concentration to inhibit the growth of mycelial colony A. flavus. Concentration 8% inhibition diameter mycelial colony A. flavus was 1.65 ± 0.2 cm with the inhibition potency of 77.82% and 10% concentration inhibtion diameter of mycelial colony A. flavus was 1.46 ± 0.2 cm with the inhibition potency of 80.37%. Research showed that the higher concentration of antifungal gave the smaller average diameter mycelial colonies and the greater percentage of the inhibition potency.
PATOGENITAS SPODOPTERA LITURA MULTIPLE NUCLEAR POLYHEDROSIS VIRUS (SPLTMNPV) YANG DILINDUNGI EKSTRAK KENCUR (KAEMPFERIA GALANGA) TERHADAP LAMA HIDUP LARVA SPODOPTERA LITURA Ainun, Irma Nur; Asri, Mahanani Tri; Rahayu, Yuni Sri
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Spodoptera litura is pest which causes leaf damage and distrub the process of photosynthesis that could result in loss of yields. This research aimed to describe the effect of adding the Kampferia galanga extract on SpltMNPV and to certain the effective concentration of Kampferia galanga extract as chemical protective material SpltMNPV of sun exposure to the length of life of S. litura larvae. The research design used was completely randomized design (CRD) with 5 treatments and 5 replications. The treatment was SpltMNPV+kaolin 1:4 which added with various concentrations of the Kampferia galanga extract 0%, 5%, 10%, 15%, and 20%. Each unit experiments used 10 S. litura larvaes. Data obtained in the form length of life of S. litura larvae were analyzed descriptively. The results showed that the treatment of SpltMNPV+kaolin 1:4 with various concentrations of Kampferia galanga extract (0%, 5%, 10%, 15%, and 20%) affected the length of life S. litura at 6 days, 6 days, 6 days, 5 days, and 5 days respectively with percentage of S. litura survive larvae at 28%, 22%, 12%, 12%, and 10% respectively. Treatment SpltMNPV + 1:4 kaolin + 15% Kampferia galanga extract was the most effective treatment in lenght of life of S. litura larvae.
PATOGENITAS SPODOPTERA LITURA MULTIPLE NUCLEOPOLYHEDROSIS VIRUS (SPLTMNPV) DENGAN BAHAN PEMBAWA TEPUNG BENGKUANG YANG TERPAPAR SINAR MATAHARI TERHADAP LAMA HIDUP LARVA SPODOPTERA LITURA Machfiroh, Aprilianti Nindya; Asri, Mahanani Tri; Rahayu, Yuni Sri
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Spodoptera litura Multiple Nucleopolyhedrosis Virus (SpltMNPV) is one of insects pathogens that infect armyworm so they can be as a natural armyworm enemies. The SpltMNPV are sensitive against the sun so that could reduce its pathogenicity level. This research aimed to determine the effective concentration of bengkuang flour to protect SpltMNPV against the sun exposure to maintain the length of life against armyworms. This research used a completely randomized design (CRD). The treatment consisted of one factors, namely bengkuang flour concentration with 4 treatments. The treatments were SpltMNPV + kaolin 1:4 added by bengkuang flour concentrations 0%, 1%, 2.5%, and 5% respectively. The result showed that SpltMNPV + kaolin 1:4 added by bengkuang flour concentrations of 0%, 1%, 2.5%, and 5% affected the length of armyworm life in 7 day, 7 day, 6 day, and 5 day respectively with percentage of armyworms 25%, 25%, 20%, and 5% respectively. The 5% concentration of bengkuang flour was the effective concentration to protect of SpltMNPV against the sun exposure.
PEMANFAATAN BAKTERI PSEUDOMONAS FLUORESCENS, JAMUR TRICHODERMA HARZIANUM DAN SERESAH DAUN JATI (TECTONA GRANDIS) UNTUK PERTUMBUHAN TANAMAN KEDELAI PADA MEDIA TANAM TANAH KAPUR Rohmah, Fuyudur; Rahayu, Yuni Sri; Yuliani, Yuliani
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

The need of area for agricultural activities and plant cultivation increase so required the using of area that has not been used effectively by farmers, such as the calcareous soil as a marginal land. This research aimed to know the effect of Pseudomonas fluorescens with various concentrations were added Trichoderma harzianum and teak leaves compost on the growth and P content of soybean plants on calcareous soil. This research method used one way analysis of variance (ANOVA), that was the P. fluorescens concentrations (0, 102, 105, 109 cfu/ml) with 6 replications. The growth parameter which measured include height, biomass, amount of leaf soybean plants. Data from growth of soybean plants was analyzed by ANOVA continued least significant difference test, then P content was analyzed by descriptively. The research result showed that P. fluorescens with various concentrations, which were added by T. harzianum and teak leaves compost, affected on the growth of soybean plants (height, biomass and amount of leaf). The concentration of 109 cfu/ml of P. fluorescens affected on the optimum growth of soybean plants. In addition, the optimum concentration of P. fluorescens on the P content of soybean plants was at 105 cfu/ml.
POLA PERTUMBUHAN POPULASI VORTICELLA GLOBOSA PADA MEDIA KULTUR AIR RENDAMAN ALANG-ALANG, BEKATUL DAN GEDEBOK PISANG DENGAN BERBAGAI KONSENTRASI Nugroho, Trias; Budijastuti, Widowati; Faizah, Ulfi
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Vorticella included in Protozoa that have an important role mainly on aquatic life, such as bioindicators, and heavy metal bioremediation agents on heavy metals contaminated waters with passive and active uptake process, therefore, necessary culture Vorticella enough to be used as bioremediator. This study aimed to describe the growth pattern of Vorticella globosa populations. This study used a completely randomized design with two treatment factors are the type of soaking culture medium of cogon grass, rice bran and banana pseudostem and concentration of 1%, 2% and 3% w/v and EL medium as well as control medium with 3 replications. The parameters measured were the Vorticella globosa population growth curve for 8 days. Vorticella globosa population growth curves were analyzed descriptively. The results showed Vorticella globosa population growth curves have the same pattern, there is a lag, log, stationary, and death phase, but the time for each phase varies.
MEDIA ALTERNATIF UNTUK PERTUMBUHAN SEL MIDGUT SPODOPTERA LITURA Aljauhari, Muhammad Ma?ruf; Asri, Mahanani Tri; Trimulyono, Guntur
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Cell culture is process when viable cell propagated in vitro in the medium. Midgut cell culture of S. litura is very useful as hosts for SpltMNPV virus that can control S. litura. This study aimed to determine the increase in the number of S. litura midgut cells and long incubation time of S. litura midgut cells in RPMI 1640, DMEM, and mixture of RPMI 1640 medium and DMEM as alternative medium with initial inoculum 4.0 x 105 cell/ml. This research used Completely Randomized Design (CRD) with 4 treatments and 3 repetitions so that there are 12 treatment unit. Data was analyzed using ANOVA (Analysis of Variance). Further tests are LSD (Least Significant Difference). The results showed that the highest increase of the number of S. litura midgut cells was obtained in Grace's medium (control) with 23125 times inoculum, the mixture of RPMI 1640 and DMEM medium was  21950 times inoculum, RPMI 1640 medium was  11725 times, DMEM medium was  10400 times inoculum. Spodoptera litura midgut cells form a monolayer in a 24-hour incubation in Grace's medium, the mixture of RPMI 1640 and DMEM medium, and DMEM, whereas the monolayer formation in RPMI 1640 medium is in a 48-hour incubation. The result showed that the mixture of RPMI 1640 and DMEM medium is an .optimum alternative medium that support the growth of Spodoptera litura midgut cell.
PENGARUH PEMBERIAN BERBAGAI KONSENTRASI KOLKHISIN TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN CABAI (CAPSICUM ANNUM) VARIETAS LADO F1 Syaifudin, Achmad; Ratnasari, Evie; Isnawati, Isnawati
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
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Pepper production in Indonesia is still very low. One effort that can be done is by using the plant breeding colchicine. Colchicine is one of the reagents for the mutation that causes polyploid, the organism has three or more sets of chromosomes in polyploid cells. The purpose of this study were to describe differences in growth and productivity in pepper (Capsicum annuum) caused by immersion in a solution colchicine sprouts at various concentrations and to determine the concentration of the most optimal colchicine?s solution influence on the growth and productivity of pepper plants as the risk of ploidisasi. The research was carried out experimentally using a randomized block design with one treatment factor is the concentration of a solution that includes 5 colchicine concentrations, ie 0 ppm (K1), 5 ppm (K2), 10 ppm (K3), 15 ppm (K4), and 20 ppm (K5). The treatment was repeated 5 times to obtain 25 units and placement experiments performed randomly in each block. Data includes pepper plant height, pepper plant biomass, pepper fruit number and papper fruit biomass statistically tested by one-way ANOVA test and if the result is significant continued using Duncan's test. The results obtained showed that the treatment of various concentrations colchicine on soaking sprouts make a difference on the growth and yield of pepper. Concentration of 15 ppm kolkhisin give optimal results on the growth and yield of pepper.
PATOGENISITAS CENDAWAN ENTOMOPATOGEN LECANICILLIUM LECANII (ZIMMERMAN) VIEGAS TERHADAP LARVA INSTAR III SPODOPTERA EXIGUA (LEPIDOPTERA: NOCTUIDAE) Wahyuni, Dianita Tri; Isnawati, Isnawati; Suparno, Gatot
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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This research aimed to describe the effect of the application of fungal L. lecanii concentration on mortality and time of death to the larvae of S. exigua. The method used were carried out an experimentally in the laboratorium and some parameters were analysed statistically using a single-factor completely randomized design with 6 concentration (105 conidia/ml, 106 conidia/ml, 107 conidia/ml, 108 conidia/ml, 109 conidia/ml and 0 conidia/ml) and 4 replications. The results showed that the application of various concentrations of an effect on mortality rates. At the concentration used was 105 conidia/ml, 106 conidia/ml, 107 conidia/ml, 108 conidia/ml and the concentration of 109 conidia/ml with a mortality rate respectively after application of 20%, 45%, 55%, 70 % and 75%. While the application of various concentrations of conidia showed that conidial concentration effect on the time of death. Each conidial concentration of 105?109 conidia/ml caused the death time is 212,4 hours, 187,2 hours, 159,6 hours, 141,6 hours and 126 hours.
PENGGUNAAN EKSTRAK DAUN SRIKAYA (ANNONA SQUAMOSA) SEBAGAI PENGENDALI JAMUR FUSARIUM OXYSPORUM SECARA IN VITRO Purwita, Ayu Anggun; Indah, Novita Kartika; Trimulyono, Guntur
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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This study aimed to determine the phytochemical profile of srikaya extract qualitatively and the antifungal activities srikaya leaves extract to inhibition on the growth of Fusarium oxysporum and find out the optimal concentration of srikaya leaves extract on inhibiting the F. oxysporum growth. The study were designed by randomized completely design with one factor treatments with 5 concentration.Antifungal activity test to be used solid dilution method. Concentration include 0% (control), 5%, 5.5%, 6%, and 6.5%. Each treatment was repeated with four replications. Observation was conducted after incubation for 7 days at room temperature. The parameters observed was inhibition F. oxysporum growth with indicated size of the diameter mycelial colony. The results showed that the srikaya leaves extract had an inhibitory effect on mycelial colony A. flavus growth. Optimum concentration in this study was 6.5% concentration because it can inhibit the growth of F. oxysporum with an average of 2,84cm ± 0,30 dimater colonies and the percentage inhibition is 60%.

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