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LenteraBio
Published by Universitas Negeri Surabaya
ISSN : 22523979     EISSN : 26857871     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 281 Documents
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PENGARUH PEMBERIAN KOMBINASI REBUSAN BIJI ALPUKAT (PERSEA AMERICANA) DAN BIJI PEPAYA (CARICA PAPAYA) TERHADAP KADAR GLUKOSA DARAH MENCIT SHOLIHAH, ALFIATUS
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

It has been done a research about the administrationof  decoction of avocado seeds (Persea americana), papaya seeds (Carica papaya) and a combination of both which aimed to decrease blood glucose levels in mice were given Dextrose monohydrate. Thirty mice were divided into 6 groups. Each group was given treatments namely: K1 (aquades), K2 (avocado seeds 100%), K3 (papaya seeds 100%), P1 (combine 40%), P2 (combine 50%) and P3 (combine 60%),  as much as 1 ml/20 g BW. Furthermore, all groups of mice were given Dextrose monohydrate 40% as much as 0,5 mL/20 g BW. Recorded data were level of fasting  blood glucose, 30 minutes post-treatment blood glucose levels, and post-Dextrose blood glucose levels. The results showed that administration of decoction combination of avocado seeds and papaya seeds can decrease blood glucose levels of mice with a Fhit 2,520 > Ftab 1.63 with p < 0.05. The lowest levels of blood glucose in mice are resulted from the administration of avocado seeds decoction treatment.   Key words: avocado seeds; papaya seeds; blood glucose levels
EFEKTIVITAS EKSTRAK DAUN CEREMAI (PHYLLANTHUS ACIDUS) TERHADAP MORTALITAS LARVA AEDES AEGYPTI CATUR PRATIWI, YULIDA
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

Aedes aegypti is an arbovirus disease vector i.e. dengue hemorrhagic fever, yellow fever, encephalitis, and chikungunya. One of the vector controlling is using botanical larvicide from Phyllanthus acidus leaves. This research aimed to describe the secondary metabolite of Phyllanthus acidus leaves extract; to describe the effect of Phyllanthus acidus leaves extract on the mortality of Aedes aegypti larvae; and to determine the lethal concentration of Phyllanthus acidus leaves extract for 50% and 90% mortality (LC50 and LC90) at 24, 48, and 72 hours after treatment. It was laboratory experimental by completely random design. The experimental object was 3rd instars of Aedes aegypti larvae that got into liquid of Phyllanthus acidus leaves extract: 0.4%; 0.6%; 0.8%; 1.0%; 1.2%; and 0% as control. There were 4 replications and every unit treatments consisted of 20 larvae. Mortality counting was held every 24 hours for 3 days, and then it was analyzed by One Way ANOVA, Tukey (HSD), and Probit. The phytochemical testing showed that Phyllanthus acidus leaves extract contain of flavonoid, tannin, and saponin. Phyllanthus acidus leaves extract significantly affected on the mortality of Aedes aegypti larvae at 24, 48, and 72 hours after treatment. LC50 and LC90 were: 0.505% and 0.922% at 24 hours after treatment; 0.432% and 0.732% at 48 hours after treatment; 0.421% and 0.682% at 72 hours after treatment.   Key words: Aedes aegypti; Phyllanthus acidus leaves; mortality of larvae; LC50; LC90
UJI BIOAKTIVITAS EKSTRAK DAUN SUREN (TOONA SINENSIS) TERHADAP MORTALITAS LARVA PLUTELLA XYLOSTELLA PADA TANAMAN SAWI HIJAU KURNIAWAN, NISA
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

The research aimed to determine the content of secondary metabolites in the leaves suren using phytochemical profile test and to determine the effect of various concentrations of leaf suren extract on mortality instar II of Plutella xylostella larvae. This research conducted in green house biology majors FMIPA UNESA with used Randomized Block Design (RBD). Suren leaves extract concentrations used were 6%, 8%, and 10%. Research data were phytochemical profiles that were analyzed descriptively, whereas the mortality of larvae of Plutella xylostella was tested by normality test prior to the test of one-way ANOVA followed by LSD test (Least Significant Difference). Phytochemical profiles obtained secondary metabolite alkaloids, flavonoids, steroids/triterpenoids and saponins, whereas mortality data showed that treatment with a concentration of 10% gave the highest influence on the mortality of Plutella xylostella of 86.3%.   Key words: bioactivity; suren leaf extracts, mortality; Plutella xylostella; green mustard
PEMANFAATAN BIJI MIMBA (AZADIRACHTA INDICA) SEBAGAI LARVASIDA NYAMUK CULEX SP. RAHMAWATI, ELMA
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

Environmentally friendly plant-based insecticides can be used incontrolling and preventing diseases caused by the Culex sp. mosquito. One of the alternative natural insecticide materialsare neem seeds (Javanese: Mimba). This study aimed to: 1). describe the effect of neem seed extract on the mortality of mosquito larvae Culex sp. 2). describe the concentrations of neem seed extract that cause mortality of mosquito larvae Culex sp.: 50% and 90%. This research was an experimental study. The larvae of Culex sp. of third instar obtained from ITD Airlangga University in Surabaya. This study used of completely randomized design with four treatments of neem seed extract concentration of 0.01%, 0.02%, 0.1%, 0.2%, 0.4% and controls with five times the repeatitions. Each treatment consisted of 20 larvae. Data were analyzed using Probit Regresion and Pearson Correlation. The results showed that the concentration of exctract that caused 50% mortality of mosquito larvae Culex sp. was 0.07172%, while the concentration of extract that caused 90% mortality larvae was 0.1927%. Pearson correlation analysis obtained the value of r = 0.922, which means that there was significant correlation between the concentrations of neem seed extract against mosquito larvae mortality of third instar Culex sp.   Key words: neem seed extract (Azadirachtaindica); mortality; Culex sp. 
PEMANFAATAN KOMPOS DAUN LAMTORO (LEUCAENA LEUCOCEPHALA) DAN DAUN ANGSANA (PTEROCARPUS INDICUS) SEBAGAI MEDIA KULTUR PERTUMBUHAN POPULASI CHAETOCEROS CALCITRANS SAFITRI, ANI
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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This study aimed to determine the effect of compost made ??from lamtoro leaves and Angsana leaves in culture medium on the growth of population C. calcitrans at variety of doses and determine the right doses compost for optimal growth of C. calcitrans. The method used in this study was experimental with completely randomized design (CRD). Research using one factor was the administration of compost treatment consisted of A (control) in the form of urea 10 mg + 5 mg TSP; B (63 mg), C (94 mg), and D (126 mg). Each treatment carried out four times, so the total sample of 16. Observational study was conducted over 8 days. Parameters measured were growth in the number of cell C. calcitrans. Data were analyzed descriptively. Based on the results obtained, it could be concluded that the mix of compost made ??from lamtoro leaves and angsana leves into the culture medium revealed the differences in the growth of C. calcitrans and the optimal doses compost that affect the population growth of C. calcitrans was treatment D (2 times the doses of compost which was 126 mg / ml).   Key words: Compost of lamtoro leaves and angsana leaves; culture medium; growth of population; Chaetoceros calcitrans
PENGARUH PEMANASAN FILTRAT RIMPANG KUNYIT (CURCUMA LLONGA) TERHADAP PERTUMBUHAN KOLONI BAKTERI COLIFORM SECARA IN VITRO NANANG FITONI, CHOLIB
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

Turmeric (Curcuma longa) is one of the potential medicinal plants, because it is useful as antioxidant and antibacterial. The purpose of this research was to describe the effect of heating turmeric on the growth of colonies of coliform bacteria in vitro. The parameter was the number of coliform bacteria colonies that grown on EMB Agar media that was used in the research. The research was carried out experimentally using a completely randomized design with 3 treatments. The first treatment was turmeric filtrate without heating. The second treatment was turmeric filtrate with temperature 90oC heating for 10 minutes. The third treatment was not using turmeric filtrate as a control. All three treatments were repeated six times to obtain the 18 treatment combinations. Parameters measured were the number of coliform bacteria colonies that grown on EMB Agar media that have given various treatments. Data analysis was using descriptive analysis. The results showed that addition of turmeric filtrate could hinder the growth of colonies of coliform bacteria. This shown by a decrease in the number of coliform bacteria colonies growing on treatment with the addition of turmeric filtrate compared to the control treatment. Giving filtrate turmeric without heating provides optimal results in inhibiting the growth of total coliform bacteria colonies.   Key words: coliform bacteria; filtrate turmeric; heating temperature
PENGARUH PEMBERIAN FILTRAT DAUN KATUK (SAUROPUS ANDROGYNUS) TERHADAP GAMBARAN HISTOPATOLOGI PARU MENCIT YANG TERPAPAR ASAP ROKOK YANTI TOGATOROP, ELZASS
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

Research has been conducted to prove that the katuk leaves filtrate can reduce the level of damage lung tissue. Sixty male mice as a subject divided into 5 groups; K0 (normal control), K1 (exposed by cigarette smoke control), P1 (exposed by cigarrete smoke and given katuk leaves filtrate 2 ml/100 gBW/day), P2 (exposed by cigarette smoke and given katuk leaves filtrate 2.5 ml/100 gBW/day), and P3 (exposed by cigrette smoke and given katuk leaves filtrate 3 ml/100 gBW/day). The cigarette smoke exposed by 1 piece smoke/1,5 hour/day. The results of the study showed the level of lung tissue damage with a value of significance (p-value<α 0,05) congestion (0,058), inflammation  (0,314), degeneration (0,366), necrosis (0,731), and total damage (0,788). It can be conclude the katuk filtrate leaves can’t repair lung tissue damage of exposure to cigarette smoke.   Key words: cigarette smoke; katuk leaves; antioxidant; lung tissue damage
PENGARUH KONSENTRASI DAN LAMA PERENDAMAN TELUR DALAM LARUTAN KOLKHISIN TERHADAP LAJU PERTUMBUHAN IKAN PATIN (PANGASIUS PANGASIUS) PRISTIA R, PRANANTA
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Catfish is one of popular fish being consumed. Therefore it requires an effort to improve fish quality productions. Colchicine is one of the reagents for mutation that causes polyploid, organisms that have three or more sets of chromosomes in their cells. Hence, they are sterile. The purpose of this study was to determine the effect of concentration of colchicine and immersion duration of eggs in colchicine solution to the growth rate of catfish. The research was conducted experimentally using a completely randomized design (CRD) with two treatment factors. The first factor was the concentration of colchicine of 0 ppm, 0.5 ppm and 1 ppm. The second was the immersion duration of eggs in the colchicine solution for 15 minutes, 20 minutes and 25 minutes. Each combination of factors was repeated three times so that there were 27 samples of treatments. The data obtained were the specific growth rate (SGR) and analyzed descriptively. The results showed that concentration of colchicine solution and egg immersion duration influence the growth rate of catfish. The highest specific growth rate was obtained by the combination of 1 ppm of colchicine concentration and 25 minutes of immersion duration.   Key words: Colchicine concentration; eggs immersion duration; specific growth rate (SGR) of catfish 
EFEKTIVITAS EKSTRAK DAUN BINAHONG (ANREDERA CORDIFOLIA) TERHADAP PENGHAMBATAN PERTUMBUHAN BAKTERI SHIGELLA FLEXNERI DENGAN METODE SUMURAN AINURROCHMAH, ANIS
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

Shigella flexneri is one of pathogenic bacteria that cause dysentery. Disease caused by Shigella sp. can be cured using antibiotics, but the excessive use of antibiotics causes bacterial resistance. One of antibiotics alternate is secondary metabolites of binahong (Anredera cordifolia) leaves. This research aimed to determine the growth inhibitory of S. flexneri strain BW 1201 and the optimal concentration of binahong leaf extracts to inhibit the growth of S. flexneri strain BW 1201. Binahong leaves extracts was prepared by maceration method, using 96% ethanol. The filtrate of binahong leaves was evaporated by vacuum rotary evaporator to obtain a viscous extract. Binahong leaf extracts tested concentration were 0% (distilled water/control), 40%, 60%, 80% dan 100% with 4 repetitions. Data obtained were the diameter of clear zone (mm) formed in the culture medium of S. flexneri strain BW 1201. Data were analyzed using one way variant analysis test and Duncan's test. Results showed that the binahong leaves extracts had inhibitory effects on the growth of S. flexneri strain BW 1201 and optimal clear zone of 27,2 mm against the test bacterial at the concentration of 100%.   Key words: Shigella flexneri; binahong leaf extracts; growth inhibitory of bacterial; well difussion method
PENGARUH PEMBERIAN EKSTRAK ETANOL BIJI MAHONI (SWIETENIA MAHAGONI) TERHADAP PENGHAMBATAN PERTUMBUHAN ESCHERICHIA COLI DENGAN METODE DIFUSI CAKRAM AGNESSYA ESA OKTAVIA, GEBBY
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 3 (2013):
Publisher : Universitas Negeri Surabaya

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Abstract

Escherichia coli is commonly known as nonpathogenic bacteria, but some strains are pathogenic and cause diarrhea, urinary tract infections, and meningitis. Escherichia coli is initially sensitive to antibiotics, but lately began resistant. Alternative antibacterial can be obtained from extracts of certain plants, such as mahogany (Swietenia mahogany). This research aimed to determine the effect of various concentrations of ethanolic extract of mahogany seeds to growth inhibition of  Escherichia coli FNCC 0091 and determine the concentration that could optimally inhibited the growth of Escherichia coli FNCC 0091. Mahogany seeds extract was prepared by maceration method with 96% ethanol. Antibacterial activity was done by disc diffusion method. Data obtained were diameter of clear zone. Data were analyzed using one-way analysis of variance followed by Duncan's test. Results showed that the ethanolic extract mahogany seeds could inhibited the growth of Escherichia coli FNCC 0091. The best treatment was ethanolic extract of mahagony seeds at concentration 100% and 80% with clear zone of 2.33 mm and 2.13 mm.     Key words: Escherichia coli FNCC 0091; ethanolic extract of mahogany seed; discs diffusion method 

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