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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 5 Documents
 1 
Search results for , issue "Vol. 14 No. 4 (2020): December 2020" : 5 Documents clear
QUALITY OF AIR BACTERIA IN OPERATING THEATER IN SOME HOSPITALS IN JAKARTA AND SURROUNDINGS AREAS IN 2018-2019 CONNY RIANA TJAMPAKASARI; NABILA NAURA; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (828.971 KB) | DOI: 10.5454/mi.14.4.3

Abstract

Nosocomial infection is an infection obtained by a patient or hospital staff while in hospital. This infectionplays a role in causing morbidity and mortality in hospitals and can occur in various hospitals rooms, includingoperating theaters. Nosocomial infections can occur due to various factors, one of which is contamination fromairborne bacteria. In some countries, regulations are set to limit the concentration of airborne bacteria, both in theoperating theaters and other rooms in hospitals, hence the need for monitoring and supervision of air quality asareflection on the cleanliness conditions in hospitals. Based on this, it is necessary to know the bacteriological airquality in the operating theaters in several hospitals in Jakarta and surrounding areas as one of the steps to preventnosocomial infections. The method uses an air sampler with the principle of impaction. Air sampler works byseparating the particles from the air by utilizing the inertia of the particles to force the bacteria to settle to thesurface of the medium. A total of 217 examinations in the operating theaters were carried out in 17 hospitals inJakarta and surrounding areas during January 2018 to June 2019. The majority of the operating theaters inhospitals in Jakarta and surrounding areas have air quality that met appropriate quality standards. In 2018, 120 of137 (87.59%) examination in the operating theaters met the quality standar. Meanwhile in 2019, 70 of 80(87.50%) operating theaters met the standard determined by the Ministry of Health of the Republic of Indonesia. Key words: air sampler method, operating theater, quality of air bacteria
Trenching Construction in Peat Soil and the DGGE Analyses of nif Gene and Activity of Dehydrogenase HAPPY WIDIASTUTI; DARMONO TANIWIRYONO; IMAN RUSMANA; GALUH WENING PERMATASARI
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2068.239 KB) | DOI: 10.5454/mi.14.4.2

Abstract

Basal stem rot (BSR) is a major disease in oil palm crops which also possible happen on peat soils. Ganoderma boninense is the pathogen that causes this disease infects the plants through the root. Maintaining root health expects to reduce the intensity of the occurrence of BSR disease and its spread. In this research, trenching construction and maintenance is carried out which aims to break the spread and activity of Ganoderma. Trenching maintenance is carried out by giving empty bunches oil palm inoculated with decomposer, Azotobacter inoculants and humic acid. Four treatments tested were trenched (T1) and untrenched (T0) in combination with two level of Ganoderma attack, which are moderate (Mo) and severe (Se). The soil sample were retrieved from different point, which are in the trenching area (TR), harvest path zone (H), and frond stack zone (M). The soil characteristic including microbe abundance, dehydrogenase activity, and PCR-DGGE of microbe community in each treatment were analyzed to reveal the effect of trenching construction. The results showed that trenching implementation reveals major results related to the abundance of microbes and fungi population, supported by the enhancement of dehydrogenase activity at the block with moderate Ganoderma attack. In addition, the DGGE study effectively separates the microbial population of four separate treatment into two clusters, splitting the grouping depending on the Ganoderma level attack. This study shows enhancement of soil characteristics biologically and nutrients status of oil palm leaves especially P, as the results of trenching construction in peatland. Keywords: peatland; oil palm; trenching, DGGE; microbes
Lactic Acid Bacteria from Tempeh and Their Ability to Acidify Soybeans in Tempeh Fermentation TATI BARUS; GABRIELA GIOVANIA; BIBIANA W LAY
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (974.342 KB) | DOI: 10.5454/mi.14.4.4

Abstract

Tempeh is the most famous traditional fermented food in Indonesia. Tempeh fermentation consists of two stages. In the first stage, the acidification of soybeans used bacteria around 24 hours. Lactic acid bacteria are found in tempeh. Therefore, this study is aimed to investigate the diversity of LAB from tempeh based on 16S rRNA gene sequences and to study their function in tempeh fermentation. In this study, twenty-two LAB isolates were obtained from tempeh. The isolates were closely related to Lactobacillus agilis, Lactobacillus fermentum, Weissella confusa, and Lactobacillus delbrueckii. L. fermentum (13 isolates) were the most abundant in tempeh, followed by L. agilis (7 isolates). It was found LAB important for the acidification of soybeans which the pH of soybean soaking water decreased from pH 7 to pH 4.4-4.9. Key words:  diversity, LAB, Lactobacillus,t empeh, Weissella
Growth Kinetic Study of Blue-green Microalgae Arthrospira platensis Using Buffalo Manure as Alternative Media BRIAN SINAGA; Lianty Simangunsong; Andy Trirakhmadi; Monita Pasaribu; Merry Martgrita
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1403.054 KB) | DOI: 10.5454/mi.14.4.5

Abstract

North Sumatera is one of the provinces in Indonesia with the highest buffalo population, which is responsible for the high accumulation of buffalo manure that can cause environmental and aesthetic problems if left untreated. One of the possible alternatives for solving this issue is by implementing buffalo manure as growth media for microorganisms, e.g. microalgae. In this research, buffalo manure was used as alternative media for Arthospira platensis cultivation. Buffalo manure was taken from Sitoluama village, Laguboti, Toba Regency of North Sumatra Province. Research steps included media and culture preparation, cultivation, sampling, sample analysis and verification of constructed models and validation. Buffalo manure concentration in media is varied from 1 g.L-1 to 8 g.L-1 which is analogous to nitrogen content of 0.002 mg.L-1 to 0.018 mg.L-1. Growth data was used for growth kinetic modelling, which was most satisfactory for Monod model (µmax = 0.5915 day-1, Ks = 0.421 g.L-1).  
The ethanol Production Activity of Indigenous Thermotolerant Yeast Pichia kudriavzevii 1P4 DAROJATUL ULYA; RIKA INDRI ASTUTI; ANJA MERYANDINI
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1227.645 KB) | DOI: 10.5454/mi.14.4.1

Abstract

Pichia kudriavzevii 1P4 is a thermotolerant-ethanologenic yeast potential for application in ethanol industry. In this study we evaluated the stress tolerance phenotype of P. kudriavzevii 1P4 in dealing with fermentation related-stresses, including high temperature stress, high sugar content, ethanol content and the fermentation capacity of the particular isolate. Based on spot assay, 1P4 showed stress tolerance phenotype against high sugar concentration for up to 30% sugar content and 10% ethanol stress. In addition, 1P4 was capable to show temperature-stress tolerance phenotype for up to 42oC, suggesting that 1P4 belong to thermotolerant yeast isolate. Fermentative activity was measured by using glucose consumption and ethanol production assay. We evaluated the fermentative and growth rate of 1P4 at various temperature condition which were 27oC, 37oC and 42oC using YPD media (at initial glucose of 2%, 10% and 20%). Interestingly, 1P4 consumed the highest glucose in 20% of concentration at 37oC (15.29%), simultaneously with the highest concentration of ethanol (32.05 g/L ethanol and 0.67 g/L/h ethanol productivity). Cell growth analysis showed that growth of 1P4 isolate increased with higher initial glucose condition yet decreased as temperature during fermentation was raised. The growth rate of 1P4 was found high in 20% initial glucose at 37oC than 2% and 10% at same temperature. In addition, 1P4 exhibited short lag phase at high-temperature fermentation. Our data indicate that 1P4 can potentially be applied as fermentation agent especially in high-temperature ethanol fermentation. Key words: bioethanol production, fermentation related-stress, thermotolerant yeast

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