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Pengaruh Estradiol-17? dan Kolagen Tipe IV Terhadap Ekspresi Gen PIK3CA untuk Menginduksi Ekspresi c-erbB2 pada Lini Sel Kanker Ovarium SKOV-3 Martgrita, Merry Meryam; Tan, Marselina Irasonia
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2312

Ovarian cancer cell metastasis is induced by signaling pathway activated by the binding of type IV collagen to ?1 integrinreceptor on the surface of cancer cells and estrogen binding to estrogen receptor. However, the role of estradiol-17? andtype IV collagen on the development of ovarian cancer have not been clearly understood. Therefore, this research wasconducted to observe the differential gene expression in SKOV-3 ovarian cancer cells cultured on type IV collagen andtreated with estradiol-17?, and incubated for 12, 24 and 72 hours. Differential display RT-PCR was used to express thedifferential expression gene after treatment. cDNA fragment that expressed differentially was isolated and sequenced.Sequencing result on one of the cDNA fragment showed that PI3K is one of the gene expressed in SKOV-3 ovarian cancercell. To verify this result, cDNA was amplified using PIK3CA specific primer. The increasing level of PIK3CA is inducedby three kinds of receptor activities, those are c-erbB2 receptor bound to estradiol-17?, homodimer receptor of c-erbB2, andthe activity of integrin receptor bound to type IV collagen. The increasing level and activity of PIK3CA can also increasethe expression of c-erbB2 gene. In SKOV-3 cells cultured on type IV collagen for 72 hours, the increasing of PIK3CA andc-erbB2 expression level is very low. The conclusion is that estradiol-17? gives a more significant effect than type IVcollagen to induce the increasing expression of PIK3CA and c-erbB2 genes.Keywords: ovarian cancer, SKOV-3, type IV collagen, estradiol-17?, PIK3CA, c-erbB2

PENGARUH ESTRADIOL-17? DAN KOLAGEN TIPE IV TERHADAP EKSPRESI GEN PIK3CA UNTUK MENGINDUKSI EKSPRESI C-ERBB2 PADA LINI SEL KANKER OVARIUM SKOV-3 Martgrita, Merry Meryam; Tan, Marselina Irasonia
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2312

Ovarian cancer cell metastasis is induced by signaling pathway activated by the binding of type IV collagen to ?1 integrinreceptor on the surface of cancer cells and estrogen binding to estrogen receptor. However, the role of estradiol-17? andtype IV collagen on the development of ovarian cancer have not been clearly understood. Therefore, this research wasconducted to observe the differential gene expression in SKOV-3 ovarian cancer cells cultured on type IV collagen andtreated with estradiol-17?, and incubated for 12, 24 and 72 hours. Differential display RT-PCR was used to express thedifferential expression gene after treatment. cDNA fragment that expressed differentially was isolated and sequenced.Sequencing result on one of the cDNA fragment showed that PI3K is one of the gene expressed in SKOV-3 ovarian cancercell. To verify this result, cDNA was amplified using PIK3CA specific primer. The increasing level of PIK3CA is inducedby three kinds of receptor activities, those are c-erbB2 receptor bound to estradiol-17?, homodimer receptor of c-erbB2, andthe activity of integrin receptor bound to type IV collagen. The increasing level and activity of PIK3CA can also increasethe expression of c-erbB2 gene. In SKOV-3 cells cultured on type IV collagen for 72 hours, the increasing of PIK3CA andc-erbB2 expression level is very low. The conclusion is that estradiol-17? gives a more significant effect than type IVcollagen to induce the increasing expression of PIK3CA and c-erbB2 genes.Keywords: ovarian cancer, SKOV-3, type IV collagen, estradiol-17?, PIK3CA, c-erbB2

Antioxidant and Antibacterial Activities Enhancement of Solid-state Fermented Candlenut Kernels by Aspergillus oryzae GRACE DOLOROSA LIMBONG; LEVY NATHANAEL NABABAN; ADELINA MANURUNG; MERRY MERYAM MARTGRITA
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

According to several studies, solid state fermentation (SSF) can enhance antioxidant and antibacterial activity of natural sources, and microorganism that is widely used in this kind of research is Aspergillusoryzae. Therefore, this study employed SSF by A. oryzae to enhance antioxidant and antibacterial activity of candlenut kernel. Candlenut kernel powder, that has been moistened with 60% water, was inoculated with 10% (w/w) of 5-day-culture ofA. oryzae, and was fermented for 9 days (until exponential phase; sample-1)and 12 days(until stationary phase; sample-2). The fermented candlenut kernels was extracted by ethanol and concentrated using rotary evaporator. Total phenolic content of control (unfermented extract), sample-1, and sample-2 are 0.183 mg GAE/g, 2.761 mg GAE/g, and 4.194 mg GAE/g, respectively. This results supported the IC50 value determined by DPPH method, those are 617.11 Î¼g/ml, 260.23 Î¼g/ml, and 45.29 Î¼g/ml. These results revealed a very strong antioxidant activity (< 50 Î¼g/ml) in the sample fermented until stationary phase.Antibacterial assay to Staphylococcus aureus resulted diameter of inhibition zone 7.17 mm, 13.51 mm, and 18.51 mm, respectively. And to Pseudomonas aeruginosa resulted diameter of inhibition zone 6.52 mm, 11.786 mm, and 15.269 mm, respectively. From this result, SSF until stationary phase enhanced higher antioxidant and antibacterial activity compared the other treatments.Â

Citric Acid Production From Toba Banana Peel (Musa acuminata Colla) Through Submerged Fermentation Using Aspergillus niger MEVA GUSTINA E. SIDAURUK; SURYA NINGSIH HUTAURUK; MERRY MERYAM MARTGRITA; ADELINA MANURUNG
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Toba banana peel waste is derived from Toba banana fruit (Musa acuminata Colla) processing. Local people utilized banana peel waste usually as livestock feed. The waste also can make an environmental problem if it is not handling well. Banana peel waste has a high content of carbohydrate that can be fermented to produce a more valuable product, one of which is citric acid. Citric acid is an organic acid that is consumed globally and produced in large quantities. In food and beverages industries, citric acid is used for various purposes due to its high solubility, non-toxic and good taste characteristics. The objective of this research is to determine the optimum conditions of submerged fermentation of banana peel to produce citric acid using Aspergillus niger. The treatments were various banana peel concentrations (5%, 10% and 15% w/v) added with 5% sucrose or 5% glucose (w/v). During the fermentation, pH was measured to determine pH changes indicated the production of citric acid. The results showed that the variation concentration of banana peel substrate and type of sugars affect citric acid production. The optimum condition of submerged fermentation by Aspergillus niger was obtained at 15% substrate concentration by adding 5% sucrose to produce 0.651% (w/v) of citric acid.

Growth Kinetic Study of Blue-green Microalgae Arthrospira platensis Using Buffalo Manure as Alternative Media BRIAN SINAGA; Lianty Simangunsong; Andy Trirakhmadi; Monita Pasaribu; Merry Martgrita
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

North Sumatera is one of the provinces in Indonesia with the highest buffalo population, which is responsible for the high accumulation of buffalo manure that can cause environmental and aesthetic problems if left untreated. One of the possible alternatives for solving this issue is by implementing buffalo manure as growth media for microorganisms, e.g. microalgae. In this research, buffalo manure was used as alternative media for Arthospira platensis cultivation. Buffalo manure was taken from Sitoluama village, Laguboti, Toba Regency of North Sumatra Province. Research steps included media and culture preparation, cultivation, sampling, sample analysis and verification of constructed models and validation. Buffalo manure concentration in media is varied from 1 g.L-1 to 8 g.L-1 which is analogous to nitrogen content of 0.002 mg.L-1 to 0.018 mg.L-1. Growth data was used for growth kinetic modelling, which was most satisfactory for Monod model (Âµmax = 0.5915 day-1, Ks = 0.421 g.L-1). Â

SCALE-UP OF PHENOLIC COMPOUNDS PRODUCTION FROM Styrax paralleloneurum LEAVES AND EXTRACT PURIFICATION USING AMBERLITE XAD-2 Martgrita, Merry Meryam; Sianipar, Imelda Febrika; Manurung, Adelina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.7431

The current study aimed to determine the total phenolic compound content, antioxidant activity, and antibacterial activity of fermented Styrax leaf extract in 2000 mL volume. Fermentation by Aspergillus niger was carried out for 64 hours. After extraction, purifi-cation was conducted using Amberlite XAD-2 resin in ion exchange chromatography. The total phenolic content of the unpurified extract was 92.75 mg GAE.g-1, while the purified extract was 146.67 mg GAE.g-1. Antioxidant activity expressed in IC50 values was 31.71 ppm for the unpurified extract and 14.11 ppm for the purified extract. The antibacterial activity tested on Staphylococcus aureus and Pseudomonas aeruginosa showed an inhibition zone diameter of 31.43 mm and 28.2 mm, respectively for the unpurified extract, while for the purified extract 33.96 mm and 35.5 mm, respectively. It can be concluded that the purification of fermented Styrax leaf extract caused a stronger antioxidant activity and a very potent antibacterial activity.

Effect of controlling the number of natural microorganisms on the taste and aroma of tuak (North Sumatra traditional beverage) Hutasoit, Sumy Ester; Turnip, Nina Juliana Roberta; Agnesia, Yoli; Martgrita, Merry Meryam
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 8, No 2 (2025)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2025.008.02.3

Tuak is a traditional North Sumatran beverage produced through the fermentation of nira, the quality of which is significantly affected by the microorganisms involved in fermentation. This study aimed to enhance the processing of nira into tuak through the addition of a controlled number of natural microorganisms that play an important role in the taste and aroma of tuak and to reach conclusions about the most liked tuak. A total of 10 mL of each starter with a concentration of 0.007 g/mL was added to a container containing sterile nira. Into C1 was added 10 mL of yeast starter, C2 was added 10 mL of LAB (lactic acid bacteria), and C3 was added 10 mL of consortia, with a volume ratio of AAB (acetic acid bacteria): LAB : yeast respectively 2:4:4, then fermented for 10 hours. The results of pH and %ethanol measurements for C1 were 4.29 and 5.94; C2 were 4.25 and 5.49; and C3 were 4.12 and 4.93. Organoleptic data were analyzed using the Friedman test and confirmed that the strategy affected the taste of tuak (p-value <0.05), but not the aroma. Furthermore, Wilcoxon test results clearly indicated that C2 was significantly different from C1 and C3. C2 was rated by panelists as the best in terms of taste and aroma profile, but C1 was also liked almost equally as a product (overall liked) compared to C3. The taste profile of C2 was sweet, with a slightly pungent sour aroma.

IDENTIFICATION OF PLANT GROWTH PROMOTING PROPERTIES OF BACTERIA ISOLATED FROM WATER HYACINTH (Eichhornia Crassipes) ROOTS Putri, Theodora Mega; Tambun, Rizka Arta Rossa; Martgrita, Merry Meryam
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.15121

Plant growth-promoting bacteria (PGPB) play an important role in enhancing plant growth and productivity through various biological mechanisms, such as phosphate solubilization and nitrogen fixation. This study aims to isolate and identify PGPB from the roots of water hyacinth (Eichhornia Crassipes) growing in Lake Toba, and to evaluate its potential in supporting the growth of lettuce (Lactuca sativa). Three rhizosphere bacteria were obtained and identified morphologically and biochemically as Bacillus thuringiensis (EGR1), Bacillus megaterium (EGR2), and Azotobacter vinelandii (EGR3). All three isolates showed the ability to solubilize phosphate, while only EGR3 was able to fix nitrogen. Bioassay tests using lettuce showed that EGR2 increased plant height, while EGR3 significantly increased fresh weight, number of leaves, and length of roots. These findings indicate the potential of EGR3 as a promising natural biofertilizer candidate to support sustainable agriculture.

Kegiatan Del Biology Competition 2022 Dalam Meningkatkan Kemampuan Pengetahuan Siswa Toba Jaya Santoso; Merry Meryam Martgrita; Asido Saragih; Sari Muthia Silalahi; Ana Muliyana
Jurnal Pengabdian Masyarakat Mandira Cendikia Vol. 3 No. 6 (2024)
Publisher : YAYASAN PENDIDIKAN MANDIRA CENDIKIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kegiatan pengabdian melalui kerjasama dan kolaborasi dibangun dan direalisasikan dalam bentuk kegiatan Olimpiade Biologi yang diselenggarakan oleh Institut Teknologi Del tahun 2022. Kegiatan olimpiade biologi untuk tingkat SMA/SMK ini difasilitasi oleh UPT Sains dan Matematika, Institut Teknologi Del, dengan harapan dapat meningkatkan mutu pendidikan sains khususnya bidang biologi melalui penumbuhkembangan budaya belajar, kreativitas, sikap disiplin, kerja keras untuk menguasai ilmu pengetahuan dan teknologi, dan motivasi meraih prestasi terbaik melalui kompetisi yang sehat serta menjunjung nilai-nilai sportivitas. Selain itu juga untuk meningkatkan kecerdasan bangsa dan kesadaran ilmiah untuk mempersiapkan generasi muda dalam menghadapi masa yang akan datang. Pada pelaksanakan kegiatan ini peserta yang telah mendaftarkan diri untuk ikut dalam tahap awal olimpiade biologi sebanyak 65 siswa yang berasal dari 27 sekolah SMA/SMK dimana babak final diikuti oleh 38 siswa yang keseluruhan berasal dari 12 sekolah. Pada kegiatan ini piala bergilir diberikan kepada sekolah yang meraih gelar juara umum yaitu SMA Unggul Del.

SCALE-UP OF PHENOLIC COMPOUNDS PRODUCTION FROM Styrax paralleloneurum LEAVES AND EXTRACT PURIFICATION USING AMBERLITE XAD-2 Martgrita, Merry Meryam; Sianipar, Imelda Febrika; Manurung, Adelina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.7431

The current study aimed to determine the total phenolic compound content, antioxidant activity, and antibacterial activity of fermented Styrax leaf extract in 2000 mL volume. Fermentation by Aspergillus niger was carried out for 64 hours. After extraction, purifi-cation was conducted using Amberlite XAD-2 resin in ion exchange chromatography. The total phenolic content of the unpurified extract was 92.75 mg GAE.g-1, while the purified extract was 146.67 mg GAE.g-1. Antioxidant activity expressed in IC50 values was 31.71 ppm for the unpurified extract and 14.11 ppm for the purified extract. The antibacterial activity tested on Staphylococcus aureus and Pseudomonas aeruginosa showed an inhibition zone diameter of 31.43 mm and 28.2 mm, respectively for the unpurified extract, while for the purified extract 33.96 mm and 35.5 mm, respectively. It can be concluded that the purification of fermented Styrax leaf extract caused a stronger antioxidant activity and a very potent antibacterial activity.

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