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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 4 Documents
 1 
Search results for , issue "Vol. 15 No. 4 (2021): December" : 4 Documents clear
Anti Fungal Activity of Chitinolytic Bacteria Lysinibacillus fusiformis and Brevibacillus reuszeri Against The Fungal Pathogens Rhizoctonia solani and Fusarium oxysporum Mashuri Masri; Eka Sukmawaty; As Awalia Amir
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.924 KB) | DOI: 10.5454/mi.15.4.3

Abstract

Abstract. Chitinolytic bacteria can produce chitinase, reported as a biocontrol agent against plants. This research aims to see chitinolytic activity in inhibiting the growth of Rhizoctonia solani and Fusarium oxysporum. Anti fungal testing in dual culture test by growing each of the chitinolytic bacteria, Lysinibacillus fusiformis and Brevibacillus reuszeri, with the pathogenic fungi, F. oxysporum and R. solani, in Petri dishes containing Chitin Agar Media facing a distance of 3 cm. The results showed that chitinolytic bacterial isolates were capable inhibit the fungus by having the activity of each index inhibition of L. fusiformis isolates (30%), B. reuszeri (77%) against F. oxysporum, and R. solani fungi isolates (100%) for each chitinolytic bacterial isolate. Keywords : Anti fungal, Chitinolytic bacteria, Pathogenic fungi.
Antibacterial and Antioxidant Activities of Ginger Essential Oils Mega Ferdina Warsito; Febriana Untari; Anggia Prasetyoputri; Fauzy Rachman; Eris Septiana; Asep Bayu; Akhirta Atikana; Linda Sukmarini; Masteria Yunovilsa Putra
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1605.595 KB) | DOI: 10.5454/mi.15.4.1

Abstract

Ginger is a rhizomatous perennial herb that grows abundantly in tropical areas. It has been used around the world as a spice, flavoring agent, and ingredient in traditional medicine. Ginger essential oils (GEOs) are derivatives of ginger that can be found in various products used in daily life, such as food, pharmaceutical, and cosmetics. The present study analyzed the chemical compositions, antioxidant, and antibacterial activities of three commercially available GEOs. The compositions of GEOs were identified using the gas chromatography method. The antioxidant activity was evaluated using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2’-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay methods. The antibacterial activity was determined using a disc diffusion assay based on the diameter of the inhibition zone (DIZ). The main compounds identified from the samples were zingiberene, α-curcumene, β-sesquiphellandrene, camphene, α-farnesene, β-bisabolene, α-pinene, and 3-carene. The IC50 values were found to be 5.3023 and 1.4504 mg/mL for GEO1; 0.9249 and 0.5276 mg/mL for GEO2; and 10.4463 and 3.3535 mg/mL for GEO3 when evaluated using DPPH and ABTS assay methods, respectively. All samples showed antibacterial activity against Staphylococcus aureus ATCC 13420 and Bacillus subtilis (collection of Indonesian Institute of Sciences), while only GEO2 and 3 displayed inhibitory effect against Escherichia coli ATCC 9637.
Relationship and structural diversity of bacterial manganese superoxide dismutases and the strategy for its application in therapy and cosmetics Debbie S. Retnoningrum; Aluicia Anita Artarini; Wangsa T. Ismaya; Abykhair Muhammad; Muhammad D. Fadilah; Ratna A. Utami
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2349.93 KB) | DOI: 10.5454/mi.15.4.2

Abstract

Manganese superoxide dismutase (MnSOD) from bacteria shares high amino acid sequence homology and nearly identical structure. Despite of that, their characteristics are diverse, which likely due to their bacterial origin and adaptation to the environment. Most importantly, their structural similarity extends to eukaryotic MnSOD, i.e. human. Therefore, structural study of bacterial MnSOD is relevant to its human SOD and henceforth for its use in human as a therapeutic agent or a cosmetic ingredient. Further, eukaryotic MnSOD occurs as a tetramer while almost all of the prokaryotic are dimeric. In this review, relationship between the amino acid sequences and structures of MnSOD as well as their origin and evolution is discussed. The structures of FeSOD and cambialistic SOD, which are MnSOD closest homologs, are visited as the comparison. This study provides an insight to potential safe application of bacterial MnSOD, including necessary modifications to obtain desired characteristics for applications in human.
Antibacterial Activity of Ethanol Extract of Portulaca oleracea L. Herb from Various Extraction Methods Against Salmonella typhimurium MAULITA CUT NURIA; AULIA EVERESTINA PUJAKA; ERIKA INDAH SAFITRI
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1020.259 KB) | DOI: 10.5454/mi.15.4.4

Abstract

Salmonella typhimurium bacteria could cause gastroenteritis and its growth could be controlled by the active compounds from natural products, which is Portulaca oleracea L. herb. Portulaca oleracea contained tannin, saponins and flavonoids compounds which had different characteristics towards temperature extraction. This study aims to determine the antibacterial activity of ethanol extract of the Portulaca oleracea herb from various extraction methods against S. typhimurium bacteria. Extraction of Portulaca oleracea herb was carried out with four variations methods which were the cold method (maceration and percolation) and heat method (soxhlet and refluxs) using 96% ethanol solvent. The four types of extract were tested for their antibacterial activity by disk diffusion at concentrations of 30%, 35%, 40%, 45% and 50% (b/v). The positive control was chloramphenicol 30 µg/disk, while the negative control was DMSO solvent. The results of antibacterial activity test in the form of zone of inhibition were statistically analyzed by Two Way Anova. The results showed that the ethanol extract of the Portulaca oleracea herb from various extraction methods had antibacterial activity against S. typhimurium. There was a significantly difference in the antibacterial activity of ethanol extract of the Portulaca oleracea herb obtained from the reflux method with other methods (maceration, percolation and soxhlet) against S. typhimurium. Keywords: ethanol extract of Portulaca oleracea L. herb, antibacterial, Salmonella typhimurium, various extraction methods

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