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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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Multiplex PCR for Rapid Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Isolated from Bandung, Indonesia IWAN SASKIAWAN; NUR HASANAH
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.1.3.4

Mycobacterium tuberculosis resistant to rifampin and isoniazid, known as multidrug-resistant M. tuberculosis (MDR-TB) strains, is an emerging problem of great importance to public health, with higher mortality rates than for drug-sensitive strains. Rifampin resistance is due to mutations on the hot spot region of the rpoB gene, especially at positions 526 and 531, and isoniazid resistance is due to mutation on katG at position 315. Mechanisms of resistance are an appropriate target for molecular genotyping diagnostic methods. Here we examined the multiplex PCR assays for the rapid detection targeting rpoB526, rpoB531, and katG mutations. Sixty-one M. tuberculosis strains were studied based on rpoB526, rpoB531, and katG315 assays employing multiplex PCR. Of the 61 strains, the susceptibility tests determined 42 isolates were MDR-TB strains, 10, 4, and 5 isolates were resistant to rifampin, isoniazid, and at least to six drugs which prescibed for TB, respectively. The mutation profiles of the 42 MDR strains assayed by multiplex PCR were 81 and 38.1% on rpoB and katG, respectively. Six rifampin-resistant isolates (60%) had a mutation on rpoB, 25% isoniazid-resistant isolates had mutation on katG, and 20% of the isolates that were sensitive to all drugs tested had a mutation on rpoB. Sequencing analysis revealed sensitivity of the multiplex PCR assay for rpoB was 98.4% and was 100% for katG. There was a 19% difference between phenotype and genotype properties of all isolates detected. In conclusion, the sensitivity of multiplex PCR method was sufficient for preliminary detection of rpoB and katG mutations, but resistance M. tuberculosis to rifampin and isoniazid were not always conferred by mutated alleles on rpoB and, especially, on katG.

Immunological Detection of Avian Influenza Virus in Infected Ducks by Monoclonal Antibodies Against AIV-H5N1 NYOMAN MANTIK ASTAWA; IDA BAGUS OKA WINAYA; LUH PUTU AGUSTINI; NINING HARTANINGSIH
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

In order to establish a detection method for avian influenza virus (AIV) infection in ducks, monoclonal antibodies (MAbs) against the virus were produced. The virus used for the production of the monoclonal antibodies was AIV-H5N1 of Indonesian origin. Immortal mouse myeloma were fused with the lymphocytes derived from the spleen of mice immunized with the virus. The MAbs were tested for their specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehyde inactivated virus and normal allantoic fluid as a negative control. Twelve MAbs which were specific against AIV were isolated and 8 of them were used for detecting of AIV antigen in duck’s tissues. AIV antigen was detected in paraffin embedded tissues of AIV-infected ducks by immunohistochemistry using MAbs. AIV antigen was not detected in ducks, which were confirmed to be AIV negative. In the infected ducks, high intensity of AIV infection was detected in proventricle gland and small intestine. The AIV antigen with a lesser intensity was also detected in lungs, spleen, and bursa of Fabricius, but hardly detected in muscle, brain, and several other issues. This study shows a clear evidence that MAbs produced in this study are applicable for use in immunological detection of AIV in infected duck tissues.

In Vitro Recombination of Poliovirus with Coxsackie A Virus Serotype 18 at Downstream Nonstructural Protein-Coding Regions ANDI UTAMA; HIROYUKI SHIMIZU
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Many genetic recombinations of poliovirus (PV) are to be found in excreted viruses, including viruses from vaccineassociated paralytic poliomyelitis (VAPP) as well as healthy vaccine recipients. Most recombinations were among different serotypes of PVs. However, recombination can also occur between PV and other enteroviruses. It was predicted that the hot spot of the recombination is in the nonstructural protein-coding regions, but the exact site is may be different in each recombination. We have demonstrated that the construct recombinant virus between PV and coxsackie A virus serotype 11 (CAV-11), or with CAV-17 with recombination site in the N-term of 2C-coding region, were viable. However, the recombination of PV with CAV-18 at this site was not viable. To determine if the recombination between PV and CAV-18 can occur at other sites, eight chimeric cDNAs (between PV [isolate PJ156] and CAV-18 [PJ156/CAV-18]), all having different recombination sites (2C-8, 2C-133, 2C-235, 2C-268, 2C-287, 2C-327, 3A-67, 3C-60) were constructed using the long-PCR method. The cDNA was then transcribed in vitro and then transfected into the HEp-2 cell-line. As expected, the recombinant virus PJ156/CAV-18, with recombination sites 2C-327, 3A-67, and 3C-60 were viable, while all the others were not. The recombinant viruses displayed a slightly smaller plaque size, but emonstrated quite similar growth as compared to the parental control PJ156. Since analysis for similarity has shown that the homology between PV and CAV-18 was high around these regions, these results supported the copy-choice mechanism of enterovirus recombination.

Selection and Identification of Cellulase-Producing Bacteria Isolated from the Litter of Mountain and Swampy Forest . WIZNA; HAFIL ABBAS; YOSE RIZAL; ABDI DHARMA; I PUTU KOMPIANG
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

The isolation and selection of cellulase-producing bacteria was conducted to identify the species of cellulolytic Bacillus. The bacteria were isolated from the litter of swampy forest in Pesisir Selatan and mountain forest in Lembah Anai Tanah Datar. These bacteria were cultivated in selective media to obtain bacteria from the genus Bacillus. Six Bacillus isolates were obtainedfrom swampy forest and three Bacillus isolates from mountain forest. These isolates were cultivated in agar medium with carboxymethylcellulose as the carbon source. Colonies which produced clear zones were assumed to be cellulolytic Bacillus. Based on biochemical and morphological examinations the result indicated that these two isolates were Bacillus coagulans and B. amyloliquefaciens. The cellulase activity of B. coagulans and B. amyloliquefaciens were 0.812 and 1 200 unit ml-1 to C1(b-exoglucanase) respectively, 0.368 and 0.488 unit ml-1 to Cx(b-endoglucanase) respectively.

Host Plant Mediated the Effect of Phosphorus on the Growth of External Hyphae of Gigaspora margarita AGUS ROHYADI
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.1.3.8

The effect of soil phosphorus (P) administered to the host plant on the growth of external hyphae of Gigaspora margarita was investigated in a glasshouse experiment using pots divided into three compartments i.e. one for donor plants (DPC), one for external fungal hyphae (EHC) and one for receiver plants (RPC) respectively. The DPC was filled with a sterilized soil/sand mix previously set up to have 5, 16 or 26 mg Bray-1 P kg-1 soil (P1, P2,or P3; categorized as low, intermediate or high level of P-availability) at pH 5.3 and inoculated with and without the fungal inoculums, while the RPC was filled with P3 without inoculation. Two pre-germinated seeds of cowpea were then grown there for 2 weeks before filling the EHC with the original sterilized soil/sand mix having pH 4.6 and 12 mg Al3+ kg-1 soil. These plants were harvested after further grown for 4-8 weeks. P fertilizer induced different growth conditions of host plants, which could control the production of external hyphae by the fungal partner. In supporting G. margarita to develop an optimum extent of external hyphae in acidic soils with a toxic level of Al3+, cowpea plants required soil P availabilities at about the intermediate level.

Isolation and Screening of Endophytic Microbes from Morinda citrifolia and their Ability to Produce Anti-Microbial Substances SHIRLY KUMALA; ENDRO BUDI SISWANTO
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Assaying for, and isolation and screening of endophytic microbes from the Morinda citrifolia plant and their ability to produce anti-microbial substances was carried out. Endophytic microbes are microorganisms that live asymptomatically within the living tissue of host plants. Microorganisms such as bacteria, fungi, and yeast can be associated with the host and produce secondary metabolites. These secondary metabolites may be enzymes and other bioactive substances with medicinal activity such as anti-arthritic, anti-cancer, and anti-microbial compounds. The aims of these experiments was to investigate the ability of endophytic microbes isolated from M. citrifolia to produce secondary metabolites which can act as anti-microbial agents. A direct seed inoculating technique was used by planting the plant sample onto the surface of Nutrient Agar and Potato-Dextrose Agar. Assessment of their ability to produce anti-microbial substances was conducted by growing the endophyte isolates in Muller Hinton Broth for bacterial isolates, and in Potato-Dextrose Yeast Broth for fungal isolates. The agar diffusion method using paper disk was applied to assay the anti-microbial activity of each substance. The results of the endophyte isolation in these experiments gave five bacterial isolates and eleven fungal isolates. All of the bacterial isolates showed a broad antimicrobial spectrum while ten of the fungal isolate demonstrated broad anti-microbial activity and four out of the ten fungal isolates had activity towards Candida albicans.

SHORT COMMUNICATION: On Scientific Publications EDUARDO AGUSTIN PADLAN
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Maybe we can make our local journals internationally “visible” and thus be included in international indices (the sign of international recognition). That may not be too difficult to achieve. If we could convince our more productive local scientists and our compatriots abroad, who are able toproduce internationally competitive results, to publish seminal papers or review papers in local journals and afterwards cite those (local) papers in their other (international) publications, then the internationalcommunity will become aware of our local science and our local journals.

The Plant – Pathogen Interactions CAHYA PRIHATNA
Microbiology Indonesia Vol. 3 No. 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

Interaction between plants and their pathogens is complex, involving multifaceted recognition of pathogens by the plants and, on the other hand, subtle evasion from the pathogens. Plants perceive pathogens through direct recognition of common molecular patterns in microbes and direct recognition of effectors or their perturbation on cellular components by the pathogens. Recognition of microbe- or pathogen-associated molecular patterns triggers innate immunity that renders plants resistant to most potential microbial pathogens. Recognition-dependant immunity in plants largely relies on polymorphism of resistance gene products that confer specificity towards host-specialised pathogens, which, in turn, induces more specific resistance that is effective against host-specialised pathogens. The deployment of effective resistance involves signalling of pathogen recognition through complex signalling cascades, transcriptional reprogramming, and defence-related genes, which all contribute to an arrest of pathogen growth. Our current insights into effector biology and to which the plants respond, provide a detailed information on the evolutionary arms race between plants and their pathogens. These will lead to an improvement of current strategies for crop improvement and protection.

Analysis of Rumen Microbial Population of Cattle Given Silage and Probiotics Using Terminal Restriction Fragment Length Polymorphism RONI RIDWAN1; YANTYATI WIDYASTUTI1; SRI BUDIARTI; ACHMAD DINOTO
Microbiology Indonesia Vol. 3 No. 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

Rumen ecology is an important observation in evaluating the effectivity of silage and probiotic additives relating to their roles in cattle productivity. The objective of this study was to examine the effects of silage and probiotics on ruminal ecosystems in vivo using a molecular approach. Terminal-restriction fragment-length-polymorphism (T-RFLP) analysis was used to detect changes of ecological communities based on 16S-ribosomal deoxyribonucleic acid (16S-rDNA). Two rumen canulated PO cattle were fed several diets i.e.; (R0) basal diet dry matter basis (Pennisetum purpureum 70% and commercial concentrate 30%), (R1) silage (basal diet fermented using Lactobacillus plantarum BTCC570), (R2) silage + probiotics (L. plantarium Str BTCC531), (R3) Basal diet + probiotics (L. plantarium Str BTCC531). Digesta samples were collected 3 h after feeding for pH and T-RFLP analysis. T-RFLP analysis was performed using the 16S-rDNA amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI, HaeIII and MspI. Results showed the effectivenes of silage and probiotics, given together, on the index of Smith and Wilson evenness applied to T-RFLP ecology data (Evar) with 0.89±0.04 being the highest. The diversity of rumen microorganisms is influenced by individual differences of each animal. T-RFLP analysis has a potency to be used for comparisons of complex bacterial communities, especially to detect changes in community structure in response to different variables and to show rumen bacteria diversity in the rumen.

Bacterial Community Profiles in the Fluid of Four Pitcher Plant Species (Nepenthes spp.) Grown in a Nursery ANDREE SIEGARA; . YOGIARA
Microbiology Indonesia Vol. 3 No. 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

Nepenthes is one of the Indonesian tropical carnivorous plants. The plant has a pitcher-like structure containing fluid for digesting insects. There are many microorganisms growing in the pitcher fluid. Different species of pitcher plants and planting sites could also contribute either to the diversity or the abundance of microorganism inhabiting the pitcher fluid. To assess the bacterial community variation in the fluid of pitcher plants grown in a nursery, amplified ribosomal DNA restriction analysis (ARDRA) was used. Four specimens of N. gracilis, N. truncata, N. veitchii and N. bicalcarata were obtained from Suska Nursery, Ciderum Village, Caringin, Bogor, Indonesia. A total of 191 positive clones were analyzed by using ARDRA. A sum of 124 phylotypes was obtained, comprising 17 in N. gracilis, 7 in N. truncata, 45 in N. veitchii and 55 in N. bicalcarata. It is interesting to note that each specimen harbored unique phylotypes, meaning that no phylotypes generated from one specimen were found in any of other specimens.

Page 20 of 40 | Total Record : 398

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Iman Rusmana

Contact Email
rusmana13@yahoo.com

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+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA